scholarly journals The Influence of Different Oregano Species on the Antioxidant Activity Determined Using HPLC Postcolumn DPPH Method and Anticancer Activity of Carvacrol and Rosmarinic Acid

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Juste Baranauskaite ◽  
Asta Kubiliene ◽  
Mindaugas Marksa ◽  
Vilma Petrikaite ◽  
Konradas Vitkevičius ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cell Line ◽  
Triple Negative ◽  
Anticancer Activities ◽  
Origanum Vulgare ◽  
Dpph Method ◽  
Ethanol Extracts ◽  
Mb231 Cell

The aim of this study was to evaluate concentration-dependent antioxidant and anticancer activities of CA and RA in ethanol extracts of three different Oregano species (Origanum onites L., Origanum vulgare L., and Origanum vulgare ssp. hirtum). The study revealed the highest RA antioxidant activity in O. vulgare ssp. hirtum (9550±95 mmol/g) and the lowest in O. vulgare L. (2605±52 mmol/g) (p<0.05). The highest CA amount was present in O. onites L., which was 1.8 and 4.7 times higher (p<0.05) than in O. vulgare ssp. hirtum and O. vulgare L., respectively. The anticancer activity was evaluated on human glioblastoma (U87) and triple-negative breast cancer (MDA-MB231) cell lines in vitro. RA anticancer activity was negligible. CA and the extracts were about 1.5–2 times more active against MDA-MB231 cell line (p<0.05) compared to U87 cell line. The anticancer activities of three tested extracts were similar against U87 cell line (p>0.05) but they had different activities against MDA-MB231 cell line.

scholarly journals Antioxidant and Anticancer Activity of Novel Derivatives of 3-[(4-Methoxyphenyl)amino]propanehydrazide

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 2980 ◽  
Author(s):  
Ingrida Tumosienė ◽  
Kristina Kantminienė ◽  
Arnas Klevinskas ◽  
Vilma Petrikaitė ◽  
Ilona Jonuškienė ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cell Line ◽  
Triple Negative ◽  
Radical Scavenging ◽  
13C Nmr Spectroscopy ◽  
Molecular Structures ◽  
Mass Spectrometry Data ◽  
Derivatives Of ◽  
Dpph Radical Scavenging

Series of novel 3-[(4-methoxyphenyl)amino]propanehydrazide derivatives bearing semicarbazide, thiosemicarbazide, thiadiazole, triazolone, triazolethione, thiophenyltriazole, furan, thiophene, naphthalene, pyrrole, isoindoline-1,3-dione, oxindole, etc. moieties were synthesized and their molecular structures were confirmed by IR, 1H-, 13C-NMR spectroscopy and mass spectrometry data. The antioxidant activity of the synthesized compounds was screened by DPPH radical scavenging method. The antioxidant activity of N-(1,3-dioxoisoindolin-2-yl)-3-((4-methoxyphenyl)amino)propanamide and 3-((4-methoxyphenyl)amino)-N’-(1-(naphthalen-1-yl)-ethylidene)propanehydrazide has been tested to be ca. 1.4 times higher than that of a well-known antioxidant ascorbic acid. Anticancer activity was tested by MTT assay against human glioblastoma U-87 and triple-negative breast cancer MDA-MB-231 cell lines. In general, the tested compounds were more cytotoxic against U-87 than MDA-MB-231 cell line. 1-(4-Fluorophenyl)-2-((5-(2-((4-methoxyphenyl)amino)ethyl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio)ethanone has been identified as the most active compound against the glioblastoma U-87 cell line.

scholarly journals Aktivitas Antioksidan serta Penghambatan HMG CoA dan Lipase dari Kombinasi Ekstrak Daun Binahong-Rimpang Temu Lawak

2019 ◽  
pp. 89-96
Author(s):  
Nanang Yunarto ◽  
Nurul Aini ◽  
Intan Sari Oktoberia ◽  
Indah Sulistyowati ◽  
Arifayu Addiena Kurniatri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Hmg Coa Reductase ◽  
Activity Test ◽  
Dpph Method ◽  
Curcuma Xanthorrhiza ◽  
Ethanol Extracts ◽  
Coa Reductase ◽  
Better Than

TThe prevalence of dyslipidemia in Indonesia continues to increase. Anredera cordifolia and Curcuma xanthorrhiza are empirically used in the community to overcome dyslipidemia disorders. Flavonoids as the main compounds in A. cordifolia and curcumin in C. xanthorrhiza have potential as antioxidant activity. This study aims to determine the in vitro mechanism of A. cordifolia and C. xanthorrhiza to reduce cholesterol associated with antioxidant activity. The testing was carried out on a combination of ethanol extracts of A. cordifolia leaves with C. xanthorrhiza rhizomes (1:1) and their single extract. The antioxidant activity test was conducted using DPPH method by making serial dilution of samples and ascorbic acid with adding DPPH. The inhibitory activity of HMG CoA and lipase enzymes was carried out enzymatically using ELISA as well as simvastatin as the comparison. The results showed that the highest antioxidant activity is in C. xanthorrhiza extract samples IC50 16.21 ± 1.74 µg/mL, but was less potential compared to ascorbic acid IC50 7,63±0,85 µg/mL. The most potential inhibition of HMG CoA and lipase is in C. xanthorrhiza samples IC50 8.35 ± 0.02 and 22.35 ± 1.26 µg/mL. The combination of A. cordifolia and C. xanthorrhiza (1: 1) is better than A. cordifolia extract but lower than C. xanthorrhiza extract in antioxidant activity as well as the inhibition of HMG CoA reductase and lipase. The result showed a correlation that the higher antioxidant activity, the higher inhibitory of HMG CoA reductase and lipase respectively.

scholarly journals Evaluation of in vitro Antioxidant and Cytotoxic Effect of Methanol Extract from aerial parts of Myristica beddomei King ssp. ustulata W.J. de Wilde

2020 ◽  
pp. 115-121
Author(s):  
Neethu Joy ◽  
Mahesh Mohan
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cytotoxic Effect ◽  
Total Phenolic ◽  
Ovarian Cancer Cells ◽  
Cytotoxicity Test ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Dpph Method

Plants are traditionally used for pharmacological activities because of its ability to produce bioactive compounds. Myristica beddomei King ssp. ustulata W.J. de Wilde is an ethnomedicinal plant and it is seen in South Western Ghats of Kerala, India. The present study assessed the phenolic content, flavonoid concentration, in vitro antioxidant and cytotoxic effect of different parts of Myristica beddomei King. The total phenolic contents in the extracts ranged from 96.29 (pericarp) to 314.47 (bark) mg g-1 gallic acid equivalent. The concentration of flavonoids in different plant part extracts ranged from 1.81   to 2.76 mg g-1 equivalent to quercetin. All the parts exhibited potential antioxidant activity with an IC50 value of 2.87 to 9.67 μg ml-1 when compared to the standard ascorbic acid with an IC50 value of 2 μg ml-1 in 1,1-diphenyl-2- picryl-hidrasil (DPPH) method. Bark showed highest activity in terms of DPPH radical scavenging (IC50 value of 2.87 µg ml-1), phosphomolybdenum test (2261.33 ± 1.65 mg g-1 trolox equivalent) and ferric ion reducing antioxidant power (FRAP) (113.1 ± 0.28 µmol Fe2+ µg-1) while pericarp showed low antioxidant activity. The in vitro screening results revealed that the seeds exhibited promising anticancer activity compared to PA1 (Ovarian Cancer) cells (50 % inhibition) were observed at a concentration 100.68 µg ml-1.  In cytotoxicity test L929 (Fibroblast) cell line compared to the other parts pericarp, mace and seed needed higher concentration (>240 µg ml-1) for LC50 value. It is a promising plant for further development of antioxidant agent as it got high content of phenolic compounds and potential antioxidant and anticancer activity.

scholarly journals Biotransformation of oleic acid and antimicrobial and anticancer activities of its biotransformatıon extracts

2019 ◽  
Vol 51 (2) ◽  
pp. 200-205
Author(s):  
Ö. Özşen Batur ◽  
Ö. Atlı ◽  
İ. Kıran
Keyword(s):  
Oleic Acid ◽  
Anticancer Activity ◽  
Cell Line ◽  
Clinical Isolate ◽  
Alternaria Alternata ◽  
Edible Oils ◽  
Biological Activities ◽  
Anticancer Activities ◽  
Ic50 Value

Oleic acid is an unsaturated fatty acid found in significant quantities in various edible oils. Scientific studies have shown that oleic acid and its derivatives exhibit a variety of biological activities including antimicrobial and anticancer activities. In the present work, biotransformation of oleic acid was carried out initially using 27 different microbial strains. Extracts obtained from biotransformation with Alternaria alternata (clinical isolate) and Aspergillus terreus var. africanus (clinical isolate) were used in antimicrobial and anticancer activity studies. The in vitro antimicrobial activities of the extracts were evaluated against 9 different pathogenic microorganisms. The results indicated that the microbial extracts were more active than oleic acid itself and showed good inhibitory activity against all tested microorganisms. In in vitro anticancer activity studies, extract 2 obtained from biotransformation with Alternaria alternata exhibited notable anticancer activity against A549 cell line with an IC50 value of 62.5 μg/ml whereas positive control cisplatin showed an IC50 value of 43.5 μg/ml. The anticancer activity of extract 2 was also found to be selective according to its higher IC50 value (122.7 μg/ml) obtained against the healthy cell line, mouse embryonic fibroblasts, NIH3T3. Due to its anticancer effect, extract 2 is considered to participate in further research.

scholarly journals SEAWEED EXTRACTS EXHIBIT ANTICANCER ACTIVITY AGAINST HeLa CELL LINES

2016 ◽  
Vol 9 (1) ◽  
pp. 114 ◽  
Author(s):  
Ashwini S. ◽  
Suresh Babut V. ◽  
Saritha . ◽  
Manjula Shantara Shantaram
Keyword(s):  
Anticancer Activity ◽  
Potential Candidate ◽  
Anticancer Activities ◽  
Vitro Assay ◽  
Anticancer Properties ◽  
Seaweed Extracts ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
Ethanol Extracts

Objective: This study was conducted to examine the anticancer activities in the extracts of marine seaweeds Gracilariacorticata.Methods: The acetone, chloroform, ethanol, methanol and aqueous extracts of collected seaweeds were tested for their anticancer properties in vitro against HeLa cancer cell lines.Results: The anticancer activity of the seaweed extracts was observed at 24hours, 48 hours and 72 h in which chloroform and ethanol extracts of G. corticata showed a greater activity with an IC50 value of 341.82 µg/ml and 244.7 µg/ml respectively for 48hours. P-values were determined by two-way analysis of variance (ANOVA). The morphology of the treated cells showed a great variation when compared to the control cells. Thus, the in vitro assay indicates that the extracts of seaweeds are the significant source of a noble anticancer agent.Conclusion: This study also infers that G. corticata could be a potential candidate for cancer therapy in the near future.

scholarly journals In-Vitro Study of Antioxidant Activities from Ethanol Extracts of Akar Kuning (Arcangelisia flava)

2019 ◽  
Vol 4 (2) ◽  
pp. 66-71
Author(s):  
Suratno Suratno ◽  
Muhammad Ikhwan Rizki ◽  
Mohammad Rizki Fadhil Pratama
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Ic50 Values ◽  
Stem Extract ◽  
Ethanol Extracts

This study aims to determine the antioxidant activity of akar kuning (Arcangelisia flava) stem extract from Central Kalimantan using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. This research includes extraction of simplicia and test of antioxidant activity by DPPH method. Based on the analysis of antioxidant activity on ethanol extract from yellow root plant stem IC50 values of 136.81 ppm were obtained. This IC50 value indicates the antioxidant strength of the yellow root plant stems including in the moderate category. Further research on antioxidant activity in the fraction of akar kuning stems needs to be done to determine the composition of the antioxidant compounds in each solvent.

scholarly journals Synthesis and Anticancer Activity of Novel Benzofurancarboxamides

2020 ◽  
Vol 10 (6) ◽  
pp. 6597-6609
Keyword(s):  
Anticancer Activity ◽  
Cell Line ◽  
National Cancer Institute ◽  
Efficient Synthesis ◽  
Anticancer Activities ◽  
Developmental Therapeutics Program ◽  
Activity Evaluation ◽  
Developmental Therapeutics

In our present work, we presented an efficient synthesis and anticancer activity evaluation of some novel benzofurancarboxamides. Our proposed approaches provide the possibility to design benzofurans diversity with a considerable chemical novelty. The synthesized substances were selected by the National Cancer Institute (NCI) Developmental Therapeutics Program for the in vitro cell line screening to investigate their anticancer activity. The compounds with significant levels of anticancer activities have been found that can be used for further optimization.

scholarly journals The Potential of Dietary Antioxidants from a Series of Plant Extracts as Anticancer Agents against Melanoma, Glioblastoma, and Breast Cancer

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1115
Author(s):  
Mindaugas Liaudanskas ◽  
Vaidotas Žvikas ◽  
Vilma Petrikaitė
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Plant Extracts ◽  
Anticancer Agents ◽  
Leaf Extract

In modern society, cancer is one of the most relevant medical problems. It is important to search for promising plant raw materials whose extracts have strong antioxidant and anticancer effects. The aim of this study was to determine the composition of phenolic compounds in plant extracts, to evaluate their antioxidant and anticancer activity, and to find the correlations between those activities. Extracts of calendula, sage, bearberry, eucalyptus, yarrow, and apple were selected for the study. The phenolic compounds of these extracts were determined by the UPLC-ESI-MS/MS method and the antioxidant activity was evaluated in vitro by four different UV-VIS spectrophotometric methods (ABTS, DPPH, CUPRAC, FRAP). The anticancer activity of extracts was tested against melanoma IGR39, glioblastoma U-87, and triple-negative breast cancer MDA-MB-231 cell lines in vitro by MTT assay. The highest content of identified and quantified phenolic compounds was found in sage leaf extract and the lowest in ethanol eucalyptus leaf extract. The highest antioxidant activity was determined by all applied methods for the acetone eucalyptus leaf extract. The majority of extracts were mostly active against the melanoma IGR39 cell line, and possessed the lowest activity against the glioblastoma U-87 cell line. Acetone extract of eucalyptus leaf samples exhibited the highest anticancer activity against all tested cell lines. Strong and reliable correlation has been found between antioxidant and anticancer activity in breast cancer and glioblastoma cell lines, especially when evaluating antioxidant activity by the FRAP method.

The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

2018 ◽  
Vol 16 (2) ◽  
pp. 127-137
Author(s):  
Paula Sofia Coutinho Medeiros ◽  
Ana Lúcia Marques Batista de Carvalho ◽  
Cristina Ruano ◽  
Juan Carlos Otero ◽  
Maria Paula Matos Marques
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Breast Adenocarcinoma ◽  
Coumaric Acid ◽  
Human Breast ◽  
The Impact

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells. </P><P> Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line. Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability. Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

Synthesis, Cytotoxicity and Antioxidant Activity of New Analogs of RC-121 Synthetic Derivatives of Somatostatin

2019 ◽  
Vol 18 (10) ◽  
pp. 1417-1424 ◽  
Author(s):  
Emilia Naydenova ◽  
Diana Wesselinova ◽  
Svetlana Staykova ◽  
Ivan Goshev ◽  
Ljubomir Vezenkov
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Synthetic Derivatives ◽  
Derivatives Of

Background: Based on the structure of RC-121 (D-Phe-c (Cys-Tyr-D-Trp-Lys-Val-Cys)-Thr-NH2, - synthetic derivatives of somatostatin), some analogs were synthesized and tested for in vitro cytotoxic and antioxidant activity. Objectives: The new analogs were modifyed at position 5 with Dap (diaminopropanoic acid), Dab (diaminobutanoic acid) and Orn and at position 6 with the unnatural amino acids Tle (t-leucine). Methods: The in vitro cytotoxic effects of the substances were investigated against a panel of human tumor cell lines HT-29 (Human Colorectal Cancer Cell Line), MDA-MB-23 (Human Breast Cancer Cell Line), Hep G-2 (Human Hepatocellular Carcinoma Cell Line) and HeLa (cervical cancer cell line). The antioxidant capacities were tested by ORAC (Oxygen Radical Antioxidant Capacity) and HORAC (Hydroxyl Radical Averting Capacity) methods. Results: All substances expressed significantly higher antioxidant capacity by comparison with galic acid and Trolox. All substances showed considerable antioxidant capacity as well. Compound 2T (D-Phe-c(Cys-Tyr-DTrp- Dap-Tle-Cys)-Thr-NH2)had the highest antioxidant effect. The compound 4T (D-Phe-c(Cys-Tyr-D-Trp- Orn-Tle-Cys)-Thr-NH2) displayed antiproliferative effect on HeLa cells with IC50 30 µM. The peptide analog 3T (D-Phe-c(Cys-Tyr-D-Trp-Lys-Tle-Cys)-Thr-NH2) exerted the most pronounced inhibition on the cell vitality up to 53%, 56% and 65% resp. against MDA-MB-23, Hep G-2, HeLa in the higher tested concentration. Conclusion: The somatostatin analogs showed moderate influence on the vitality of different tumor cells and could be used in changing their pathology.

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