scholarly journals Association of High Cardiovascular Fitness and the Rate of Adaptation to Heat Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Małgorzata Żychowska ◽  
Alicja Nowak-Zaleska ◽  
Grzegorz Chruściński ◽  
Ryszard Zaleski ◽  
Jan Mieszkowski ◽  
...  
Keyword(s):  
Heat Stress ◽  
Cardiorespiratory Fitness ◽  
Adaptive Response ◽  
Interleukin 10 ◽  
Cardiovascular Fitness ◽  
Blood Samples ◽  
Qrt Pcr ◽  
Genes Expression ◽  
Before And After ◽  
Adaptation To Heat

This study aimed to compare changes in genes expression associated with inflammation and apoptosis in response to heat stress caused by sauna between people with varying cardiorespiratory fitness levels. We hypothesis that high cardiorespiratory level caused higher positive changes after four weeks of sauna bathing. Blood samples were taken at rest before and after the first and last sauna sessions and 48 hours after the last sauna session and used to assay HSP70 (HSPA1A), HSP27 (HSPB1), interleukin 6 (IL6), and interleukin 10 (IL10) genes expression in blood with quantitative real-time qRT-PCR. Overall, small decreases in rest values of HSPA1A and IL6 mRNA, increase in HSPB1 mRNA, and a significant increase in IL10 mRNA were observed after four weeks of exposure to heat stress. Our findings suggest that an adaptive response to heat stress (an anti-inflammatory response) occurs faster in people with higher cardiorespiratory fitness.

Angiogenic biomaker study in osteosarcoma

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e21507-e21507
Author(s):  
P. C. Pavoni-Ferreira ◽  
A. S. Petrilli ◽  
M. T. Alves ◽  
R. Jesus-Garcia Filho ◽  
S. R. Toledo
Keyword(s):  
Metronomic Chemotherapy ◽  
High Dose Chemotherapy ◽  
Marker Genes ◽  
High Dose ◽  
Lower Grade ◽  
Vegf Receptor ◽  
Blood Samples ◽  
Genes Expression ◽  
Before And After ◽  
Prospective Assessment

e21507 Background: This study represents a prospective assessment of angiogenesis genes mRNA expression in tumors and blood from patients treated with pre- and post-operative Brazilian osteosarcoma protocol (GCBTO 2006) that introduce metronomic chemotherapy (anti-angiogenic) in order to try to increase survival of osteosarcoma patients. Methods: Tumor samples from 27 patients were analyzed before and after chemotherapy to determine VEGFA, VEGFR1, VEGFR2, PDGFC, SDF1 and TSP1 genes expression profile by Quantitative Real Time PCR. Also, blood samples of these patients were investigated pre- and post-chemotherapy and at the end of high-dose chemotherapy trying to evaluate potential for proangiogenic factors and antiangiogenic factor (TSP1) which could be used to monitor treatment activity. Results: Of all six genes studied pre- and post- chemotherapy, in tumor samples, only SDF1 and VEGFR2 were underexpressed. SDF1 gene has the lowest expression at all. In tumor samples, TSP1 and VEGFA expression was higher than SDF1, VEGFR2 and PDGFC expression in biopsies and surgeries (P=0.001). VEGFR1 expression was higher than VEGFR2 expression (P=0.001). PDGFC and VEGFR1 overexpression were associated with necrosis grade I and II (Huvos score) (P=0.005). VEGFA and TSP1 were overexpressed in 96% and 92% of surgery samples, respectively. In blood samples from biopsy, surgery and end of treatment there were no statistically significant changes in the marker genes expression. Conclusions: The study suggests an association between PDGFC and VEGFR1 overexpression and lower grade necrosis. TSP1 and VEGFA were the most expressed genes in all tumor samples but TSP1 was lower than VEGFA in biopsies and VEGFA was lower than TSP1 in surgery (P=0.001). Although VEGFR2 is the primary receptor of VEGF, VEGFR1 was the most expressed VEGF receptor. No significant financial relationships to disclose.

scholarly journals The Impact of Dehydration and Hyperthermia on Circulatory Glutathione Metabolism after Exercise in the Heat with Insights into the Role of Erythrocytes

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1144
Author(s):  
Denise de Melo-Marins ◽  
Juliano Boufleur Farinha ◽  
Franccesco Pinto Boeno ◽  
Alexandra Ferreira Vieira ◽  
Samuel Vargas Munhoz ◽  
...  
Keyword(s):  
Heat Stress ◽  
Redox Balance ◽  
Water Bath ◽  
Glutathione Metabolism ◽  
Blood Samples ◽  
Before And After ◽  
Different Temperatures ◽  
The Impact

Background: Reduced glutathione (GSH) is one of the main thiols involved in antioxidant defense. Changes in circulatory levels of GSH during exercise are associated with hyperthermia and dehydration. The mechanisms by which these alterations occur are not entirely known. We hypothesize that erythrocytes could be an important source of circulatory GSH during heat stress conditions. We performed two separate experiments to address this hypothesis. Methods: In the first experiment, we sought to investigate the impact of exercise in the heat and dehydration on erythrocyte levels of GSH. A total of 10 men performed 60 min of cycling at 60% VO2peak in the heat (38.0 ± 0.9 °C) or in a control temperate environment (23.0 ± 1.0 °C), both with and without dehydration. Relative humidity ranged from 50 to 70%. Blood samples were taken before and after exercise to measure GSH and oxidized (GSSG) glutathione. In the second experiment, erythrocytes were isolated from blood samples taken at rest and heated in vitro to determine the impact of heat on erythrocyte glutathione content. Tubes with erythrocytes were exposed to water baths at different temperatures; one tube was exposed to a water bath at 35 °C and the other tube to a water bath at 41 °C for a period of 30 min. After exposure to heat, plasma and erythrocytes were extracted for GSH and GSSG analyses. Results: Dehydration decreased circulatory GSH, regardless of ambient temperature (temperate and heat decreased 15.35% and 30.31%, respectively), resulting in an altered redox balance. Heat increased GSH levels in vitro. Conclusion: Our data suggest that dehydration decreases circulatory GSH levels regardless of environmental temperature. In addition, in vitro data suggests that erythrocytes may contribute to the release of GSH during exposure to heat stress.

Dietary Changes in Fasting Levels of Factor VII Coagulant Activity (FVII: C) Are Accompanied by Changes in Factor VII Protein and other Vitamin K-dependent Proteins

1995 ◽  
Vol 73 (02) ◽  
pp. 239-242 ◽  
Author(s):  
E M Bladbjerg ◽  
T Tholstrup ◽  
P Marckmann ◽  
B Sandström ◽  
J Jespersen
Keyword(s):  
Fatty Acids ◽  
Vitamin K ◽  
Protein C ◽  
Saturated Fatty Acids ◽  
Factor Vii ◽  
Dietary Regimen ◽  
Blood Samples ◽  
Dietary Changes ◽  
Coagulant Activity ◽  
Before And After

SummaryThe mechanisms behind dietary effects on fasting coagulant activity of factor VII (FVII: C) are not clarified. In the present study of 15 young volunteers, two experimental diets differing in composition of saturated fatty acids (C18:0 [diet S] or C12:0 + C14:0 [diet ML]) were served for 3 weeks each. Fasting blood samples were collected before and after the dietary regimen and analysed for triglycerides, FVII:C, and protein concentrations of FVII, FII, FX, protein C, CRP, albumin, fibrinogen, and F1+2. FVII:C was significantly reduced on diet S compared with diet ML. This was accompanied by a decrease in FVII protein, F1+2 and the vitamin K-dependent proteins FII, FX, and protein C. In contrast, no changes were observed in triglycerides, FVII:C/FVII: Ag, albumin and CRP. Fibrinogen was increased on diet S compared with diet ML. Our findings suggest that the change in fasting FVII:C was part of a general change in concentrations of vitamin K-dependent proteins.

ADRENAL 17-HYDROXYCORTICOSTEROID SECRETION IN THE DOG IN RESPONSE TO ETHANOL

1972 ◽  
Vol 70 (4) ◽  
pp. 736-740 ◽  
Author(s):  
T. Suzuki ◽  
R. Higashi ◽  
T. Hirose ◽  
H. Ikeda ◽  
K. Tamura
Keyword(s):  
Venous Blood ◽  
Secretion Rate ◽  
Conscious Dogs ◽  
Blood Samples ◽  
Before And After

ABSTRACT Conscious dogs were infused intravenously with ethanol in doses of 0.7 and 1.0 g/kg. The adrenal venous blood samples were collected before and after the infusion of ethanol and analysed for 17-hydroxycorticosteroids (17-OHCS). After the infusion of 0.7 g/kg (subanaesthetic dose) of ethanol the adrenal 17-OHCS secretion rate showed either a slight increase or no change. After the infusion of 1.0 g/kg (anaesthetic dose) of ethanol the adrenal 17-OHCS secretion rate increased markedly and reached 1.21±0.15 (mean±sem) μg/kg/min, while it was 0.09±0.023 μg/kg/min before the infusion.

STUDY OF CXCL12, CCR4, EGFR GENE EXPRESSION IN MIGRATING MYELOMONOBLASTIC LEUKEMIA CELLS BEFORE AND AFTER CHEMOTHERAPY

2019 ◽  
Vol 1 (1) ◽  
pp. 100-104
Author(s):  
A. B. Filina ◽  
O. A. Svitich ◽  
Yu. I. Ammur ◽  
A. K. Golenkov ◽  
E. F. Klinushkina ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Mononuclear Cells ◽  
Boyden Chamber ◽  
External Stimulation ◽  
Healthy Donors ◽  
Genes Expression ◽  
Before And After ◽  
Chemokine Cxcl12 ◽  
And Migration ◽  
Cell Chemotaxis

Аim. A study of CXCL12 effect on the migration of mononuclear cells isolated from healthy patients, from patients with myelomonoblastic leukemia before and after chemotherapy and the study of CCR4, EGFR and CXCL12 genes expression after exposure to CXCL12. Materials and methods. The chemotaxis of mononuclear cells (MNCs) of healthy donors and patients with myelomonoblastic leukemia was studied in a Boyden chamber, followed by isolation of RNA, reverse transcription and PCR-RV. Results. A significant increase in myelomonoblasic cell chemotaxis towards CXCL12 after chemotherapy was demonstrated, as well as a decrease in the expression of this chemokine in tumor cells before chemotherapy after exposure to CXCL12. Сonclusion. Presumably, the tumor cells themselves produce CXCL12 in large amounts, which is necessary for the disturbance of intercellular interactions and further intravasation, whose production may decrease with external stimulation by the same chemokine. CXCL12 also helps to increase the expression level of EGFR and CCR4, which leads to increased tumor proliferation and migration of tumor cells.

scholarly journals Effects of cooled floor pads combined with chilled drinking water on behavior and performance of lactating sows under heat stress

2021 ◽  
Vol 99 (3) ◽  
Author(s):  
Y Zhu ◽  
L J Johnston ◽  
M H Reese ◽  
E S Buchanan ◽  
J E Tallaksen ◽  
...  
Keyword(s):  
Drinking Water ◽  
Heat Stress ◽  
Respiration Rate ◽  
Rectal Temperature ◽  
Time Budget ◽  
Video Recording ◽  
Lactating Sows ◽  
Before And After ◽  
And Performance ◽  
Drinking Frequency

Abstract This study was conducted to evaluate whether cooled floor pads combined with chilled drinking water could alleviate negative impacts of heat stress on lactating sows. Thirty sows (Landrace × Yorkshire, Parity = 1 to 6) were housed in individual farrowing stalls in two rooms with temperatures being controlled at 29.4°C (0700–1900 hours) and 23.9°C (1900–0700 hours). Sows in one room (Cool), but not in the other room (Control) were provided cooled floor pads (21–22°C) and chilled drinking water (13–15°C). Behavior of sows (15 sows/treatment) was video recorded during farrowing, and days 1, 3, 7, 14, and 21 after farrowing. Videos were viewed continuously to register the birth time of each piglet, from which total farrowing duration and birth intervals were calculated. The number of drinking bouts and the duration of each drinking bout were registered for each sow through viewing videos continuously for 2 h (1530–1730 hours) each video-recording day. Postures (lying laterally, lying ventrally, sitting, and standing) were recorded by scanning video recordings at 5-min intervals for 24 h each video-recording day, and time budget for each posture was calculated. Rectal temperature and respiration rate were measured for all sows the day before and after farrowing, and then once weekly. Sow and litter performance was recorded. Data were analyzed using the Glimmix procedure of SAS. The cooling treatment did not affect sow behavior or litter performance. Sows in the Cool room had lower rectal temperature (P = 0.03) and lower respiration rate (P < 0.001), consumed more feed (P = 0.03), tended to have reduced weight loss (P = 0.07), and backfat loss (P = 0.07) during lactation than sows in the Control room. As lactation progressed, sows increased drinking frequency (P < 0.001) and time spent lying ventrally (P < 0.0001), standing (P < 0.001), and sitting (P < 0.0001), and decreased time spent lying laterally (P < 0.0001) in both Cool and Control rooms. While cooled floor pads combined with chilled drinking water did not affect sow behavior, they did alleviate heat stress partially, as indicated by decreased rectal temperature, respiration rate, weight, and backfat loss, and increased feed intake in lactating sows.

scholarly journals Correction to: cardiorespiratory fitness in adolescents before and after the COVID-19 confinement: a prospective cohort study

2021 ◽  
Author(s):  
Rubén López-Bueno ◽  
Joaquín Calatayud ◽  
Lars Louis Andersen ◽  
José Casaña ◽  
Yasmín Ezzatvar ◽  
...  
Keyword(s):  
Cohort Study ◽  
Cardiorespiratory Fitness ◽  
Prospective Cohort Study ◽  
Prospective Cohort ◽  
Before And After

Exercise Training and the Speed of Self-Selected Walking Pace in Men at Retirement

1986 ◽  
Vol 5 (1) ◽  
pp. 19-26 ◽  
Author(s):  
D. A. Cunningham ◽  
P. A. Rechnitzer ◽  
A. P. Donner
Keyword(s):  
Exercise Training ◽  
Training Program ◽  
Walking Speed ◽  
Exercise Group ◽  
Cardiovascular Fitness ◽  
Blue Collar ◽  
Intervention Trial ◽  
Before And After ◽  
Exercise Endurance ◽  
One Year

ABSTRACTAn intervention trial with regular physical activity was carried out to test the hypothesis that walking speed is related to the level of cardiovascular fitness (maximal oxygen uptake). Earlier research has demonstrated that when VO2 max is held constant in a multiple regression analysis the association between walking speed and age (29–65 y) is eliminated. To describe the association between self-selected speeds of walking, and level of cardiovascular fitness, 63 men age 60 to 65 were studied before and after a 1 year program of exercise training. VO2 max was determined on a motor driven treadmill. A test of self-selected walking pace was administered over a 240 m indoor course. The men were asked to walk at 3 paces considered by the subjects to be rather slow, normal, and fast, respectively. They were randomly assigned with stratification for white and blue collar occupation to a program of exercise training (n = 33) or control (n = 27). The training program consisted of walking or jogging for 30 min three times per week for one year. After the training program the exercise group increased their VO2 max (9.5%) and their normal walking speed (1.29 to 1.43 m.s−1) significantly compared to the controls. A program of exercise endurance training will result in an increase in the speed of self-selected pace.

The comparative performance of tropical crossbred and local shorthorn beef cattle on native pasture at Katherine, NT

1967 ◽  
Vol 7 (26) ◽  
pp. 217 ◽  
Author(s):  
MJT Norman
Keyword(s):  
Heat Stress ◽  
Body Temperature ◽  
Peanut Meal ◽  
Average Weight ◽  
Wet Season ◽  
Comparative Performance ◽  
Native Pasture ◽  
Weight Gains ◽  
Adaptation To Heat ◽  
Dressing Percentage

The liveweight performance and carcase characteristics of Brahman x Hereford, Africander x Hereford and Africander x Shorthorn F, crossbred spayed heifers were compared with those of local Shorthorn spayed heifers on native pasture at Katherine, N.T., between 1962 and 1965. The breed groups were divided for planes of winter nutrition, viz., with and without 2 lb a day of peanut meal from early June until the start of the wet season. Without supplement, the average weight gains of B x H, A x H, A x S, and Shorthorn cattle between June 1962 and May 1965 were 0.49, 0.37, 0.36, and 0.23 lb a day respectively. With supplement, cattle reached slaughter weight a year earlier ; the average weight gains of B x H, A x H, and Shorthorn cattle between June 1962 and May 1964 were 0.61, 0.63, and 0.50 lb a day respectively. (There was no A x S supplemented group). Without supplement, the dressing percentage and estimated proportion of fat of B x H cattle was higher and the estimated proportion of muscle and bone lower than those of other breeds. With supplement, there were no significant differences in dressing percentage or carcase composition between breeds. Measurements made of thermoregulatory attributes indicated that the local Shorthorn cattle, through body temperature control, showed adaptation to heat stress.

INFLUENCE OF PHOTOPERIOD ON THYROTROPIN RELEASING HORMONE-INDUCED PROLACTIN RELEASE IN EWES

1983 ◽  
Vol 63 (1) ◽  
pp. 67-73 ◽  
Author(s):  
B. E. HOWLAND ◽  
D. SONYA ◽  
L. M. SANFORD ◽  
W. M. PALMER
Keyword(s):  
Thyrotropin Releasing Hormone ◽  
Day Length ◽  
Constant Light ◽  
Serum Prolactin ◽  
Prolactin Release ◽  
Blood Samples ◽  
Releasing Hormone ◽  
Before And After ◽  
Release Curve ◽  
Short Days

The influence of photoperiod on serum prolactin levels and prolactin release induced by thyrotropin releasing hormone (TRH) was determined in ewes maintained under the following lighting regimes: Room 1, lighting mimicked natural changes in photoperiod; Room 2, annual photoperiod changes condensed into 6 mo with short days in June; Room 3, same as Room 2 except photoperiod changed abruptly from 16.5 to 8.0 h on 21 Mar. and back to 16.5 h on 21 June; Room 4, constant light. Weekly blood samples were obtained from February to August. Additionally, blood samples were collected before and after treatment with 10 μg TRH on 19 May, 13 June, 27 June and 19 July. Prolactin levels were elevated in ewes exposed to long days or constant light. The mean of all pre-TRH samples was significantly correlated with stress-induced elevations in prolactin (highest pre-TRH value) (r = 0.72) and area under the TRH-induced release curve (r = 0.56). The prolactin release in response to TRH was greatest in ewes exposed to long days or constant light. Abrupt increase of day length elevated pretreatment prolactin levels (P < 0.01) and increased area under the response curve (P < 0.05). Key words: Photoperiod, TRH, prolactin, ewes

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