scholarly journals Antioxidant Properties of Fullerene Derivatives Depend on Their Chemical Structure: A Study of Two Fullerene Derivatives on HELFs

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Vasilina Sergeeva ◽  
Olga Kraevaya ◽  
Elizaveta Ershova ◽  
Larisa Kameneva ◽  
Elena Malinovskaya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chemical Structure ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Lung Fibroblasts ◽  
Human Embryonic Lung ◽  
Fullerene Derivatives ◽  
The Past ◽  
Wide Range ◽  
Short Times

Oxidative stress is a major issue in a wide number of pathologies (neurodegenerative, cardiovascular, immune diseases, and cancer). Because of this, the search for new antioxidants is an important issue. One of the potential antioxidants that has been enthusiastically discussed in the past twenty years is fullerene and its derivatives. Although in aqueous solutions fullerene derivatives have shown to be antioxidants, their properties in this regard within the cells are controversially discussed. We have studied two different water-soluble fullerene C60 and C70 derivatives on human embryonic lung fibroblasts at a wide range of concentrations. Both of them cause a decrease in cellular ROS at short times of incubation (1 hour). Their prolonged action, however, is fundamentally different: derivative GI-761 causes secondary oxidative stress whereas derivative VI-419-P3K keeps ROS levels under control values. To gain a better understanding of this effect, we assessed factors that could play a role in the response of cells to fullerene derivatives. Increased ROS production occurred due to NOX4 upregulation by GI-761. Derivative VI-419-P3K activated the transcription of antioxidant master regulator NRF2 and caused its translocation to the nucleus. This data suggests that the antioxidant effect of fullerene derivatives depends on their chemical structure.

Antioxidant Properties of a New Water-Soluble Fullerene C70 Derivative

2020 ◽  
Vol 854 ◽  
pp. 223-229
Author(s):  
Ekaterina A. Savinova ◽  
Elizaveta S. Ershova ◽  
Olga A. Kraevaya ◽  
Pavel A. Troshin ◽  
Svetlana V. Kostyuk
Keyword(s):  
Antioxidant Properties ◽  
Water Soluble ◽  
Lung Fibroblasts ◽  
Fullerene Derivative ◽  
Oxygen Species ◽  
Embryonic Lung ◽  
Human Embryonic Lung ◽  
Fullerene Derivatives ◽  
Biosafety Assessment ◽  
Toxic Concentrations

The search for effective antioxidants is an urgent medical and scientific problem, since oxidative stress causes a number of diseases and accelerates aging of human body. In this paper, we analyzed the ability of a new water-soluble fullerene C70 (F350) derivative to bind reactive oxygen species (ROS) when it was added to human embryonic lung fibroblasts (HELF). We found its non-toxic concentrations for cells, since the fullerene derivatives biosafety assessment is important for the potential application of the nanocomposites in medicine and biology. We also investigated the ability of this fullerene derivative to pass the cell membrane.

Genotoxic Effect of a New Water-Soluble Fullerene Derivative C70 and its Effect on the Transcriptional Activity of Genes that Regulate the Cell Cycle, DNA Repair and Apoptosis

2021 ◽  
Vol 1031 ◽  
pp. 222-227
Author(s):  
Ekaterina A. Savinova ◽  
Elizaveta S. Ershova ◽  
Olga A. Kraevaya ◽  
Pavel A. Troshin ◽  
S.V. Kostyuk
Keyword(s):  
Cell Cycle ◽  
Transcriptional Activity ◽  
Water Soluble ◽  
Lung Fibroblasts ◽  
Fullerene Derivative ◽  
Embryonic Lung ◽  
Dna Breaks ◽  
Human Embryonic Lung ◽  
Fullerene Derivatives ◽  
Cellular Dna

It is important to take into consideration the new fullerene derivatives genotoxicity. In the present is study, we analyzed the new water-soluble fullerene C70 (F350) effects on the human embryonic lung fibroblasts (HELF) oxidative damage and DNA breaks. We found that the studied compound causes cellular DNA damage and affects the transcriptional activity of cell cycle and cell apoptosis regulating genes.

scholarly journals Solid-state studies and antioxidant properties of the γ-cyclodextrin·fisetin inclusion compound

2017 ◽  
Vol 13 ◽  
pp. 2138-2145 ◽  
Author(s):  
Joana M Pais ◽  
Maria João Barroca ◽  
Maria Paula M Marques ◽  
Filipe A Almeida Paz ◽  
Susana S Braga
Keyword(s):  
Inclusion Compound ◽  
Antioxidant Properties ◽  
Physicochemical Analysis ◽  
Water Soluble ◽  
Solid Inclusion ◽  
Human Consumption ◽  
X Ray Diffraction ◽  
Wide Range ◽  
Soluble Solid ◽  
Cp Mas Nmr

Fisetin is a natural antioxidant with a wide range of nutraceutical properties, including antidiabetic, neuroprotecting, and suppression or prevention of tumors. The present work describes the preparation of a water-soluble, solid inclusion compound of fisetin with gamma-cyclodextrin (γ-CD), a cyclic oligosaccharide approved for human consumption. A detailed physicochemical analysis of the product is carried out using elemental analysis, powder X-ray diffraction (PXRD), Raman, infrared and 13C{1H} CP-MAS NMR spectroscopies, and thermal analysis (TGA) to verify fisetin inclusion and to present a hypothetical structural arrangement for the host–guest units. The antioxidant activity of the γ-CD·fisetin inclusion compound is evaluated by the DPPH assay.

Oxygen and environmental stress in plants: and evolutionary context

1994 ◽  
Vol 102 ◽  
pp. 155-165 ◽  
Author(s):  
George A. F. Hendry
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Environmental Stress ◽  
Angiosperm Evolution ◽  
Selective Pressures ◽  
The Past ◽  
Wide Range ◽  
Evolutionary Context ◽  
Radical Processes ◽  
Oxidative Attack

SynopsisContemporary plant species show a wide range of responses to oxidative attack. Much of this variation may reflect the different environmental selective pressures operating at different geological periods over the course of angiosperm evolution. Evidence is provided to show that the wide range of contemporary responses to oxidative stress may directly reflect the persistence of genes controlling free radical processes under environments of the past.

Curcuma longa extract reduces the risk of oxidative stress during intense physical exertion

2020 ◽  
pp. 43-49
Author(s):  
A. A. Khisamova ◽  
O. A. Gizinger
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Literature Review ◽  
Curcuma Longa ◽  
Antioxidant Properties ◽  
Physical Exertion ◽  
The Body ◽  
Irreversible Processes ◽  
Oxygen Species ◽  
Wide Range

Increased physical exertion is a catalyst for oxidative stress and the production of reactive oxygen species, which entails irreversible processes in the body, leading to chronic diseases and disability. This article contains a literature review of studies that prove the effect of the antioxidant properties of Curcuma longa on cells under oxidative stress. To search for data, a wide range of literature and databases was explored: Pubmed, Google.Scholar, and Embase.

scholarly journals Polyhydroxyfullerene Binds Cadmium Ions and Alleviates Metal-Induced Oxidative Stress in Saccharomyces cerevisiae

2014 ◽  
Vol 80 (18) ◽  
pp. 5874-5881 ◽  
Author(s):  
Arunava Pradhan ◽  
José Paulo Pinheiro ◽  
Sahadevan Seena ◽  
Cláudia Pascoal ◽  
Fernanda Cássio
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Interactive Effects ◽  
Membrane Disruption ◽  
Dependent Manner ◽  
Extracellular Medium ◽  
Cadmium Ions ◽  
Ph Dependent

ABSTRACTThe water-soluble polyhydroxyfullerene (PHF) is a functionalized carbon nanomaterial with several industrial and commercial applications. There have been controversial reports on the toxicity and/or antioxidant properties of fullerenes and their derivatives. Conversely, metals have been recognized as toxic mainly due to their ability to induce oxidative stress in living organisms. We investigated the interactive effects of PHF and cadmium ions (Cd) on the model yeastSaccharomyces cerevisiaeby exposing cells to Cd (≤5 mg liter−1) in the absence or presence of PHF (≤500 mg liter−1) at different pHs (5.8 to 6.8). In the absence of Cd, PHF stimulated yeast growth up to 10.4%. Cd inhibited growth up to 79.7%, induced intracellular accumulation of reactive oxygen species (ROS), and promoted plasma membrane disruption in a dose- and pH-dependent manner. The negative effects of Cd on growth were attenuated by the presence of PHF, and maximum growth recovery (53.8%) was obtained at the highest PHF concentration and pH. The coexposure to Cd and PHF decreased ROS accumulation up to 36.7% and membrane disruption up to 30.7% in a dose- and pH-dependent manner. Two mechanisms helped to explain the role of PHF in alleviating Cd toxicity to yeasts: PHF decreased Cd-induced oxidative stress and bound significant amounts of Cd in the extracellular medium, reducing its bioavailability to the cells.

Automated Method for Determining Amylase Activity in Serum and Urine

1973 ◽  
Vol 19 (10) ◽  
pp. 1187-1192 ◽  
Author(s):  
A Mazzuchin ◽  
C Weggel ◽  
C J Porter
Keyword(s):  
Amylase Activity ◽  
Water Soluble ◽  
Standard Curve ◽  
The Past ◽  
Automated Method ◽  
Wide Range ◽  
On Line ◽  
Starch Substrate ◽  
Suspended Substrate

Abstract We describe an automated procedure for determination of α-amylase activity in serum or urine by use of a water-insoluble chromogenic starch substrate (commercially available). "AutoAnalyzer" (Technicon Corp.) equipment is used in which the sample and suspended substrate are introduced simultaneously into the flow system. After incubation at 37 °C the water-soluble blue-starch breakdown products are filtered on-line across a cellulose nitrate membrane. Absorbance is measured at 630 nm, and the α-amylase activity read from a standard curve. The automated procedure has been in use for the past year and has been found to be an operationally simple, reproducible, and reliable method, which can be applied over a wide range of enzyme activity. Results of comparative studies with other α-amylase methods are also described.

Curcumin-C3 Complexed with α-, β-cyclodextrin Exhibits Antibacterial and Antioxidant Properties Suitable for Cancer Treatments

2020 ◽  
Vol 20 (12) ◽  
pp. 988-1001 ◽  
Author(s):  
Desu N. K. Reddy ◽  
Ramya Kumar ◽  
Shao-Pin Wang ◽  
Fu-Yung Huang
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Diseases ◽  
Curcuma Longa ◽  
Antioxidant Properties ◽  
Preventive Treatment ◽  
Entrapment Efficiency ◽  
Cancer Treatments ◽  
Molar Ratios ◽  
Transmission Electron ◽  
Wide Range

Background: The curcumin-C3 (cur-C3) complex obtained from Curcuma longa rhizome is a combination of three curcuminoids, namely, curcumin, dimethoxycurcumin, and bisdemethoxycurcumin. Cur and curcuminoids have been extensively researched for their wide range of therapeutic properties against inflammatory diseases, diabetes, and cancer. Objective: In spite of their extensive medicinal properties, cur and curcuminoids have poor solubility and bioavailability due to their hydrophobicity. This limitation can be overcome by complexing cur-C3 with natural cyclic oligosaccharides, such as Cyclodextrin (CD). Methods: In this study, cur-C3 and CD (α, β) inclusion complexes (ICs) were prepared with different molar ratios and characterized by nuclear magnetic resonance, Fourier transform infrared spectroscopy, X-ray diffraction, and transmission electron microscopy. Results: The cur-C3 cyclodextrin ICs showed an increased entrapment efficiency of 97.8% and improved antioxidant activity compared to cur and can be used as an antioxidant to reduce cancer-related oxidative stress. Additionally, α- CD ICs of curcumin-C3 caused an increase in growth inhibition of Staphylococcus aureus. Conclusion: Our findings suggest that both α- and β-CDs are suitable carriers for cur-C3 and can be used as an effective treatment for cancer-associated oxidative stress and as a preventive treatment for nosocomial infections and pneumonia.

scholarly journals Glycol Chitosan: A Water-Soluble Polymer for Cell Imaging and Drug Delivery

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4371 ◽  
Author(s):  
Fengming Lin ◽  
Hao-Ran Jia ◽  
Fu-Gen Wu
Keyword(s):  
Antimicrobial Agents ◽  
Tumor Imaging ◽  
Water Soluble ◽  
Glycol Chitosan ◽  
Water Soluble Polymer ◽  
The Past ◽  
Wide Range ◽  
Microbial Infections ◽  
Functional Biomaterials ◽  
Shed Light

Glycol chitosan (GC), a water-soluble chitosan derivative with hydrophilic ethylene glycol branches, has both hydrophobic segments for the encapsulation of various drugs and reactive functional groups for facile chemical modifications. Over the past two decades, a variety of molecules have been physically encapsulated within or chemically conjugated with GC and its derivatives to construct a wide range of functional biomaterials. This review summarizes the recent advances of GC-based materials in cell surface labeling, multimodal tumor imaging, and encapsulation and delivery of drugs (including chemotherapeutics, photosensitizers, nucleic acids, and antimicrobial agents) for combating cancers and microbial infections. Besides, different strategies for GC modifications are also highlighted with the aim to shed light on how to endow GC and its derivatives with desirable properties for therapeutic purposes. In addition, we discuss both the promises and challenges of the GC-derived biomaterials.

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