scholarly journals Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Edgar Ricardo Vázquez-Martínez ◽  
Claudia Bello-Alvarez ◽  
Ana Lorena Hermenegildo-Molina ◽  
Mario Solís-Paredes ◽  
Sandra Parra-Hernández ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Content ◽  
Progesterone Receptors ◽  
Reproductive Age ◽  
Eutopic Endometrium ◽  
Protein Levels ◽  
Progesterone Resistance ◽  
Gynecological Diseases ◽  
Membrane Progesterone Receptors ◽  
Ectopic Endometrium

Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by nongenomic mechanisms via membrane progesterone receptors (mPRs) that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of mPR members in the eutopic and ectopic endometrium of women with endometriosis. Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 (mPRα), PAQR8 (mPRβ), and PAQR6 (mPRδ) at mRNA and protein levels was evaluated by RT-qPCR and Western blot, whereas PAQR5 (mPRγ) gene expression was evaluated by RT-qPCR. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey’s multiple comparisons test with a confidence interval of 95 %. The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.

scholarly journals Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis

2020 ◽  
Author(s):  
EDGAR RICARDO VAZQUEZ-MARTINEZ ◽  
Claudia Bello-Álvarez ◽  
Ana Lorena Hermenegildo-Molina ◽  
Mario Solís-Paredes ◽  
Sandra Parra-Hernández ◽  
...  
Keyword(s):  
Protein Content ◽  
Progesterone Receptors ◽  
Reproductive Age ◽  
Eutopic Endometrium ◽  
Protein Levels ◽  
Progesterone Resistance ◽  
Gynecological Diseases ◽  
Membrane Progesterone Receptors ◽  
One Way Anova ◽  
Ectopic Endometrium

Abstract Background: Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by non-genomic mechanisms via membrane progesterone receptors that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of PAQR family members in the eutopic and ectopic endometrium of women with endometriosis. Methods: Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 ( mPRα ) , PAQR8 , (mPRβ) PAQR5 ( mPRγ ) , and PAQR6 ( mPRδ ) at mRNA and protein levels was evaluated by RT-qPCR and western blot. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey's multiple comparisons test with a confidence interval of 95%. Results: The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Conclusions: Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.

scholarly journals Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis

2020 ◽  
Author(s):  
EDGAR RICARDO VAZQUEZ-MARTINEZ ◽  
Claudia Bello-Alvarez ◽  
Ana Lorena Hermenegildo-Molina ◽  
Mario Solís-Paredes ◽  
Sandra Parra-Hernandez ◽  
...  
Keyword(s):  
Protein Content ◽  
Progesterone Receptors ◽  
Reproductive Age ◽  
Eutopic Endometrium ◽  
Protein Levels ◽  
Progesterone Resistance ◽  
Gynecological Diseases ◽  
Membrane Progesterone Receptors ◽  
One Way Anova ◽  
Ectopic Endometrium

Abstract Background: Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by non-genomic mechanisms via membrane progesterone receptors that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of PAQR family members in the eutopic and ectopic endometrium of women with endometriosis. Methods : Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 ( mPRα ) , PAQR8 , (mPRβ) PAQR5 ( mPRγ ) , and PAQR6 ( mPRδ ) at mRNA and protein levels was evaluated by RT-qPCR and western blot. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey's multiple comparisons test with a confidence interval of 95%. Results : The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Conclusions : Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.

scholarly journals Differential Expression of MicroRNAs between Eutopic and Ectopic Endometrium in Ovarian Endometriosis

2010 ◽  
Vol 2010 ◽  
pp. 1-29 ◽  
Author(s):  
Nicoletta Filigheddu ◽  
Ilaria Gregnanin ◽  
Paolo E. Porporato ◽  
Daniela Surico ◽  
Beatrice Perego ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Expression ◽  
Posttranscriptional Regulation ◽  
Noncoding Rna ◽  
Critical Role ◽  
Regulation Of Gene Expression ◽  
Differentially Expressed ◽  
Ovarian Endometriosis ◽  
Eutopic Endometrium ◽  
Ectopic Endometrium

Endometriosis, defined as the presence of endometrial tissue outside the uterus, is a common gynecological disease with poorly understood pathogenesis. MicroRNAs are members of a class of small noncoding RNA molecules that have a critical role in posttranscriptional regulation of gene expression by repression of target mRNAs translation. We assessed differentially expressed microRNAs in ectopic endometrium compared with eutopic endometrium in 3 patients through microarray analysis. We identified 50 microRNAs differentially expressed and the differential expression of five microRNAs was validated by real-time RT-PCR in other 13 patients. We identifiedin silicotheir predicted targets, several of which match the genes that have been identified to be differentially expressed in ectopicversuseutopic endometrium in studies of gene expression. A functional analysis of the predicted targets indicates that several of these are involved in molecular pathways implicated in endometriosis, thus strengthening the hypothesis of the role of microRNAs in this pathology.

Vitamin D3 induces the expression of membrane progesterone receptors (mPRs) on naive CD4+ T lymphocyte cells in women of reproductive age

2019 ◽  
Vol 72 ◽  
pp. 55-61
Author(s):  
Mitra Rafiee ◽  
Mohsen Naseri ◽  
Maryam Akbari-Fakhrabadi ◽  
Narges Motamedi ◽  
Ataollah Ghahiri ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Vitamin D3 ◽  
Progesterone Receptors ◽  
Reproductive Age ◽  
Women Of Reproductive Age ◽  
Membrane Progesterone Receptors

scholarly journals GRN, NOTCH3, FN1, and PINK1 expression in eutopic endometrium – potential biomarkers in the detection of endometriosis – a pilot study

2020 ◽  
Vol 37 (11) ◽  
pp. 2723-2732
Author(s):  
Isabell Holzer ◽  
Amanda Machado Weber ◽  
Anne Marshall ◽  
Alexander Freis ◽  
Julia Jauckus ◽  
...  
Keyword(s):  
Gene Expression ◽  
Laparoscopic Surgery ◽  
Protein Expression ◽  
Molecular Mechanisms ◽  
Reproductive Age ◽  
Tissue Samples ◽  
Protein Levels ◽  
Clinical Biomarkers ◽  
Mrna Gene ◽  
Severity Of The Disease

Abstract Purpose Endometriosis (EM) is a common gynecological disease affecting 10–15% of women of reproductive age. However, molecular mechanisms and pathogenesis are still not completely understood. Furthermore, due to the absence of a reliable clinical biomarker, the only viable method for the often-delayed definitive diagnosis is laparoscopic surgery. Our objective was to analyze molecular differences of selected endometrial proteins and genes of women suffering from different stages of EM compared with healthy women to evaluate potential clinical biomarkers. Methods We analyzed eutopic endometrial tissue samples from women undergoing a laparoscopic surgery (n = 58). mRNA gene expression of progranulin (GRN), neurogenic locus notch homolog protein (NOTCH3), fibronectin (FN1), and PTEN-induced kinase 1 (PINK1) was analyzed using qRT-PCR. Protein expression was determined using ELISA and immunohistochemistry. Results Significant differences in gene expression between the different stages of the disease were noted for GRN, NOTCH3, FN1, and PINK1 (p < 0.05). The endometrium of women with minimal EM (ASRM I) showed the highest mRNA expression. Protein levels of GRN and FN1 on the other hand were significantly decreased in the endometrium of women with EM compared with those of healthy controls. Furthermore, for GRN and FN1, we could detect a correlation of protein expression with the severity of the disease. Conclusion Our findings suggest a potential use of GRN and FN1 as clinical biomarkers to detect endometriosis. In addition, GRN, NOTCH3, FN1, and PINK1 could potentially be useful to differentiate between the underlying stages of the disease. However, a validation with a larger study population is needed.

scholarly journals Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Ruihua Zhong ◽  
Aying Ma ◽  
Jianping Zhu ◽  
Guoting Li ◽  
Shuwu Xie ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Protein Expression ◽  
Rat Model ◽  
Expression Profiles ◽  
Serum Levels ◽  
Dependent Manner ◽  
Western Blot Assay ◽  
Eutopic Endometrium ◽  
Protein Levels ◽  
Ectopic Endometrium

We evaluated the effectiveness of Kuntai Capsule (KTC) for treating endometriosis using rat model and investigated its preliminary mechanism of action involved. SD rats were implanted with endometrial tissues and treated with KTC for three weeks. Then, laparotomy was performed to examine volume changes of the autografts. The serum levels of TNF-α, IL-6, COX-2, E2, and P4 were measured through ELISA. TUNEL was performed to analyze the apoptosis on ectopic endometrium. Protein levels of caspases 8, 9, and 3 and cytochrome c in the ectopic and eutopic endometrium were measured by western blotting. Results showed that KTC significantly decreased the volumes of ectopic endometrium. The level of TNF-αincreased and E2decreased in the KTC treatment groups. TUNEL and western blot assay showed that KTC could induce apoptosis of endometriotic tissues, accompanied with the increased protein expression of caspases 8 and 9, activated caspase-3, and cytochrome c in a dose-dependent manner. However, these protein expression profiles were not affected in eutopic endometrium. Our findings suggest that KTC could inhibit the growth of ectopic endometrial tissue through upregulating the level of TNF-αand its downstream signaling, including caspases and cytochrome c.

scholarly journals Effect of progesterone on the MGMT gene expression in MCF7, HEp-2 and 293 cells

2021 ◽  
Vol 18 (1-2) ◽  
pp. 16-21
Author(s):  
Z. M. Nidoieva ◽  
A. P. Latsyshyna
Keyword(s):  
Gene Expression ◽  
Dna Methyltransferase ◽  
Gene Expression Regulation ◽  
Expression Patterns ◽  
Progesterone Receptors ◽  
Protein Isolation ◽  
Mgmt Gene ◽  
Methylguanine Dna Methyltransferase ◽  
Protein Levels ◽  
293 Cells

Aims: to investigate the steroid hormone progesterone effect on the human MGMT gene expression at the mRNA and protein levels in cell lines with different expression patterns of the nuclear progesterone receptors and membrane receptor PGRMC1. Methods: cell culture, RNA / protein isolation, cDNA synthesis, real-time polymerase chain reaction, Western blot analysis. Results: We observe the MGMT gene upregulation by progesterone at both mRNA and protein levels. Conclusions the effect of progesterone on MGMT expression is more complex than direct regulation through the classical nuclear receptor.Keywords: O6-methylguanine-DNA methyltransferase (MGMT), progesterone, nuclear progesterone receptors (nPR), progesterone receptor membrane component 1 (PGRMC1), gene expression regulation.

scholarly journals Gene expression analysis of membrane progesterone receptors in women with recurrent spontaneous abortion: a case control study

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Reyhane Rahnama ◽  
Mitra Rafiee ◽  
Saloomeh Fouladi ◽  
Maryam Akbari-Fakhrabadi ◽  
Ferdos Mehrabian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spontaneous Abortion ◽  
Progesterone Receptors ◽  
Recurrent Spontaneous Abortion ◽  
Case Group ◽  
Relative Expression ◽  
Significant Difference ◽  
History Of ◽  
Membrane Progesterone Receptors ◽  
Fertile Women

Abstract Objective Recurrent spontaneous abortion (RSA) is a condition which is defined as three consecutive pregnancy losses prior to 20 weeks from the last menstrual period. Progesterone is a steroid hormone that has an essential role in the implantation and maintenance of pregnancy. The progesterone signaling is performed by nuclear progesterone receptors (NPRs) and membrane progesterone receptors (mPR). The aim of this study was to analyze gene expression of mPR-α, mPR-β and NPR in the endometrium of patients with a history of RSA compared to normal fertile women. Results In this study, endometrial samples were obtained from 10 women with a history of RSA and 10 fertile women during days 10–14 of menstrual cycle. Relative expression of mPR-α, mPR-β and NPR genes were studied by a quantitative real time polymerase chain reaction (qRT-PCR) and compared between the two groups. The mean relative expression of mPR-β gene was significantly lower in the case group compared to the fertile women (p < 0.05). However, the gene expression of mPR-α and NPR showed no significant difference between two groups. The findings suggest a reduction of endometrial gene expression of mPR-β in RSA patients may play an important role in pathogenesis of RSA.

scholarly journals PPARy Expression in Eutopic and Ectopic Endometrium of Reproductive Age Women with Endometriosis

2016 ◽  
Author(s):  
Adya F Dilmy ◽  
Muharam Natadisastra ◽  
Kanadi Sumapradja
Keyword(s):  
Obstet Gynecol ◽  
Cross Sectional Study ◽  
Reproductive Age ◽  
Cross Sectional ◽  
Eutopic Endometrium ◽  
Reproductive Age Women ◽  
Keywords Endometriosis ◽  
Two Samples ◽  
Qpcr Method ◽  
Ectopic Endometrium

Objective: To evaluate the expression of PPARy receptor and to compare its expression in eutopic and ectopic endometrium in women with endometriosis. Method: This is a cross sectional study. Ten female subjects with endometriosis that underwent laparoscopy or laparotomy who fulfilled the inclusion criteria were recruited by consecutive sampling. Two samples were taken, eutopic endometrium and ectopic endometrium from endometriosis cyst wall during surgery of each subject. PPARy expression was examined by two-step RT-qPCR. Our data was statistically examined using the paired t-test and Pearson’s correlation test. Result: PPARy was found to be expressed in eutopic and ectopic endometrium of women with endometriosis using the RT-qPCR method. The expression of PPARy was not statistically different in eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative fold; p=0.26). By Pearson’s correlation there was a weak positive correlation between PPARy expression of eutopic and ectopic endometrium (r=0.16). Conclusion: PPARy was detected by two-step RT-qPCR in eutopic and ectopic endometrium of women with endometriosis. Semiquantification of PPARy expression showed that there was no significant difference between PPARy expression in eutopic and ectopic endometrium of women with endometriosis. There was a weak positive correlation between PPARy expression in eutopic and ectopic endometrium of women with endometriosis. [Indones J Obstet Gynecol 2015; 3-4: 200-205] Keywords: endometriosis, PPARy, two-step RT-qPCR

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