Effect of a Traditional Chinese Medicine Formula (CoTOL) on Serum Uric Acid and Intestinal Flora in Obese Hyperuricemic Mice Inoculated with Intestinal Bacteria

CoTOL is a traditional Chinese medicine (TCM) formula in clinics for treating gout and hyperuricemia, especially in obese patients with recurrent attacks. However, fewer studies have investigated how CoTOL impacts the intestinal flora in reducing uric acid. In the present, we analyze the bacteria targeted by ingredients of CoTOL and evaluate the effects of CoTOL on uric acid and intestinal flora in a mice model of obese hyperuricemia inoculated with xanthine dehydrogenase- (XOD-) producing bacteria, Streptococcus faecalis. Firstly, ingredients of herbs in CoTOL and gene target by these ingredients were retrieved from TCMID 2.0, and these genes were screened by DAVID Bioinformatics Resources 6.8, deciphered to retrieve the bacteria. Then, 3-4-week-old male C57bl/6j mice were randomly divided into 6 groups and fed with high fat diet for 8 weeks up to obesity standard. The mice were inoculated intragastrically with 5 × 109 CFU Streptococcus faecalis 3 times at the 5th, 6th, and 7th week and intragastrically administrated with uricase inhibitor, potassium-oxonate (PO, 250 mg/kg), to induce hyperuricemia at the 8th week, once a day for 7 consecutive days, respectively (IB model). IB model plus CoTOL (0.4 ml/20g) and allopurinol (40 mg/kg) were administrated by gavage at the 5th week, once a day for 4 weeks. The feces and blood in each group were sampled at the 4th and 8th week. With no bacteria inoculation, CoTOL, allopurinol, and blank group were treated with CoTOL and allopurinol or water, respectively. 44 species of bacteria (i.e., Enterococcus faecalis, Streptococcus, etc.) genes were targeted by 6 ingredients of 6 herbs in CoTOL. Inoculation with Streptococcus faecalis significantly caused the elevation of uric acid and the change of intestinal flora structure, whereas treatment with CoTOL signiﬁcantly increased the abundance of Akkermansia and those of Bacteroides and Alloprevotella decreased. Furthermore, CoTOL exhibited a unique effect on reducing weight unobserved in allopurinol intervention. The present study, for the first time, demonstrated that CoTOL has beneficial effects on hyperuricemia and overweight, which may be attributed to regulating material metabolism and improving the structure or function of intestinal flora. Thus, CoTOL may be a promising therapy for hyperuricemia and overweight in chronic gout management and can be integrated with conventional treatments.

Download Full-text

Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

Microbial Cell Factories ◽

10.1186/s12934-020-01473-3 ◽

2020 ◽

Vol 19 (1) ◽

Author(s):

Sihan Li ◽

Youcheng He ◽

Haiou Zhang ◽

Rong Zheng ◽

Ruoying Xu ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Relative Abundance ◽

Intestinal Flora ◽

Intestinal Bacteria ◽

Symbiotic Relationships ◽

Study Results ◽

Rrna Sequencing ◽

Pcr Technology

AbstractIn this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the intestinal flora. We found that the relative abundance of Firmicutes with constipation was significantly higher accounted for 86.7%, while the gut microbiota was significantly changed after taking a certain dose of Chinese medicine, greatly increased the relative abundance of Lactobacillus accounted for 23.1%, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.

Download Full-text

Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

10.21203/rs.3.rs-36144/v1 ◽

2020 ◽

Author(s):

Minghan Huang ◽

Sihan Li ◽

Youcheng He ◽

Haiou Zhang ◽

Ruoying Xu ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Relative Abundance ◽

Intestinal Flora ◽

Intestinal Bacteria ◽

Control Group ◽

Symbiotic Relationships ◽

Study Results ◽

Significant Difference ◽

Abundance And Diversity

Abstract In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the abundance and diversity of intestinal flora in rats. We found that the relative abundance of Firmicutes in the intestinal flora of rats with constipation was significantly higher than that in the control group, and the Bacteroides was significantly lower than that in the control group, while the relative abundance of Firmicutes was significantly changed after taking a certain dose of Chinese medicine, and reduced the relative abundance of Lachnospiraceae, and greatly increased the relative abundance of Lactobacillus, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. There was no significant difference in the total copies of intestinal bacteria between the constipated rats and the control group. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.

Download Full-text

Nigella A ameliorates inflammation and intestinal flora imbalance in DSS induced colitis mice

AMB Express ◽

10.1186/s13568-020-01114-3 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Xingjiang Hu ◽

Nana Xu ◽

Xi Yang ◽

Xi Hu ◽

Yunliang Zheng ◽

...

Keyword(s):

Ulcerative Colitis ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Inflammatory Response ◽

Therapeutic Effect ◽

Antioxidant Activities ◽

Intestinal Flora ◽

Signs And Symptoms ◽

Microbial Composition ◽

Bacterial Genus

Abstract Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and Sulfasalazine (SASP) could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.

Download Full-text

Hypouricemic Effects of Phenylpropanoid Glycosides Acteoside ofScrophularia ningpoensison Serum Uric Acid Levels in Potassium Oxonate-Pretreated Mice

The American Journal of Chinese Medicine ◽

10.1142/s0192415x08005667 ◽

2008 ◽

Vol 36 (01) ◽

pp. 149-157 ◽

Cited By ~ 20

Author(s):

Cai Guo Huang ◽

Yan Jun Shang ◽

Jun Zhang ◽

Jian Rong Zhang ◽

Wen Jie Li ◽

...

Keyword(s):

Uric Acid ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Xanthine Oxidase ◽

Serum Uric Acid ◽

Mouse Liver ◽

Xanthine Dehydrogenase ◽

Phenylpropanoid Glycoside ◽

Phenylpropanoid Glycosides ◽

Potassium Oxonate

Phenylpropanoid glycoside acteoside was extracted from the traditional Chinese medicine Scrophularia ningpoenis Hemsl. In the present study, we investigated the effects of acteoside administration on serum uric acid levels in mice rendered hyperuricemic with the uricase inhibitor potassium oxonate. When administered orally for 3 days at doses of 50, 100 and 150 mg/kg, acteoside reduced serum uric acid levels by 15.2, 23.8 and 33.1%, respectively, relative to vehicle-treated hyperuricemic mice. Importantly, in non-hyperuricemic mice, the serum uric acid levels were not affected by acetoside treatment. Acteoside also inhibited mouse liver xanthine dehydrogenase XDH and xanthine oxidase XO activity at all three doses. These results suggest that the hypouricemic action of acteoside may be attributable to its inhibition of XDH/XO activity.

Download Full-text

Pemberian Akupresur Untuk Menurunkan Nyeri Dan Kadar Asam Urat Pada Klien Asam Urat Di Poncowarno Kecamatan Kalirejo Lampung Tengah

JURNAL KREATIVITAS PENGABDIAN KEPADA MASYARAKAT (PKM) ◽

10.33024/jkpm.v4i3.2842 ◽

2021 ◽

Vol 4 (3) ◽

pp. 515-519

Author(s):

Teguh Pribadi ◽

Ainur Rahma ◽

Rika Yulendasari

Keyword(s):

Uric Acid ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Healing Process ◽

Nutritional Therapy ◽

Joint Disease ◽

Herbal Therapy ◽

Keywords Pain ◽

Nonpharmacological Therapy

ABSTRAKPada tahun 2018, prevalensi penyakit sendi di Lampung berada pada urutan ke-12 di Indonesia yaitu sebesar 7,2%. Terapi nonfarmakologi untuk mempercepat proses penyembuhan pada pasien hiperurisemia yaitu terapi herbal, hemeopati, akupuntur, akupresur, terapi nutrisi, refleksologi, terapi garam, dan yoga. Akupresur merupakan salah satu teknik pengobatan tradisional Cina ang dapat digunakan untuk menurunkan nyeri, mengobati penyakit dan cidera. Tujuan setelah penyuluhan dan demonstrasi, diharapkan pemberian akupresur dapat untuk menurunkan kadar asam urat dan rasa nyeri pada klien Asam urat tinggi. Adapun kegiatan yang dilakukan berupa penyuluhan menggunakan leaflet dan demonstrasi akupresur. Terdapat penurunan kadar asam urat dan nyeri pada klien asam urat tinggi setelah pemberian akupresur selama 7 hari di Poncowarno Kecamatan Kalirejo, Lampung Tengah. Dengan demikian, pemberian akupresur pada klien asam urat sangat efektif dalam menurunkan kadar asam urat dan nyeri. Kata Kunci: Nyeri, Akupresur, Asam Urat ABSTRACTIn 2018, the prevalence of joint disease Lampung ranks 12th at Indonesia (7.2%). Nonpharmacological therapy to accelerate the healing process in hyperuricemia's patients that is herbal therapy, hemeopathy, acupuncture, acupressure, nutritional therapy, reflexology, salt therapy, and yoga. Acupressure is one of the traditional Chinese medicine techniques which can be used to reduce pain, treat illnesses and injuries. The purpose after counseling and demonstration, it is expected that the provision of acupressure can reduce uric acid levels and pain in high uric acid's client. The activities carried out in the form of counseling using leaflets and acupressure demonstrations. There was a decrease in uric acid levels and pain in high uric acid's clients after giving acupressure for 7 days at Poncowarno, Kalirejo District, Central Lampung. Thus, giving acupressure to uric acid clients is very effective in reducing uric acid levels and pain. Keywords: Pain, Acupressure, Gout

Download Full-text

Role of intestinal flora characteristics in traditional Chinese medicine-based diagnosis and treatment of spleen and stomach diseases

World Chinese Journal of Digestology ◽

10.11569/wcjd.v27.i10.605 ◽

2019 ◽

Vol 27 (10) ◽

pp. 605-610

Author(s):

Yun-Shan He ◽

Hua-Ying Hui ◽

Zhou-Jin Tan

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Intestinal Flora ◽

Diagnosis And Treatment ◽

Stomach Diseases

Download Full-text

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Frontiers in Pharmacology ◽

10.3389/fphar.2021.604435 ◽

2021 ◽

Vol 12 ◽

Author(s):

Shimaa M. A. Sayed ◽

Karsten Siems ◽

Christian Schmitz-Linneweber ◽

Walter Luyten ◽

Nadine Saul

Keyword(s):

Caenorhabditis Elegans ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Eucommia Ulmoides ◽

Pumping Rate ◽

C Elegans ◽

Beneficial Effects ◽

Physiological Fitness ◽

Pharyngeal Pumping ◽

Cuscuta Chinensis

To uncover potential anti-aging capacities of Traditional Chinese Medicine (TCM), the nematode Caenorhabditis elegans was used to investigate the effects of Eucommia ulmoides and Cuscuta chinensis extracts, selected by screening seven TCM extracts, on different healthspan parameters. Nematodes exposed to E. ulmoides and C. chinensis extracts, starting at the young adult stage, exhibited prolonged lifespan and increased survival after heat stress as well as upon exposure to the pathogenic bacterium Photorhabdus luminescens, whereby the survival benefits were monitored after stress initiation at different adult stages. However, only C. chinensis had the ability to enhance physical fitness: the swimming behavior and the pharyngeal pumping rate of C. elegans were improved at day 7 and especially at day 12 of adulthood. Finally, monitoring the red fluorescence of aged worms revealed that only C. chinensis extracts caused suppression of intestinal autofluorescence, a known marker of aging. The results underline the different modes of action of the tested plants extracts. E. ulmoides improved specifically the physiological fitness by increasing the survival probability of C. elegans after stress, while C. chinensis seems to be an overall healthspan enhancer, reflected in the suppressed autofluorescence, with beneficial effects on physical as well as physiological fitness. The C. chinensis effects may be hormetic: this is supported by increased gene expression of hsp-16.1 and by trend, also of hsp-12.6.

Download Full-text

Intestinal Flora: A Pivotal Role in Investigation of Traditional Chinese Medicine

The American Journal of Chinese Medicine ◽

10.1142/s0192415x21500130 ◽

2021 ◽

pp. 1-32

Author(s):

Xiao Li ◽

Dan Wu ◽

Jingjie Niu ◽

Yanping Sun ◽

Qiuhong Wang ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Human Health ◽

Life Science ◽

Science And Technology ◽

Intestinal Flora ◽

Pivotal Role ◽

Unique Role ◽

The Core ◽

Host Health

Intestinal flora is essential for maintaining host health and plays a unique role in transforming Traditional Chinese Medicine (TCM). TCM, as a bodyguard, has saved countless lives and maintained human health in the long history, especially in this COVID-19 pandemic. Pains of diseases have been removed from the effective TCM therapy, such as TCM preparation, moxibustion, and acupuncture. With the development of life science and technology, the wisdom and foresight of TCM has been more displayed. Furthermore, TCM has been also inherited and developed in innovation to better realize the modernization and globalization. Nowadays, intestinal flora transforming TCM and TCM targeted intestinal flora treating diseases have been important findings in life science. More and more TCM researches showed the significance of intestinal flora. Intestinal flora is also a way to study TCM to elucidate the profound theory of TCM. Processing, compatibility, and properties of TCM are well demonstrated by intestinal flora. Thus, it is no doubt that intestinal flora is a core in TCM study. The interaction between intestinal flora and TCM is so crucial for host health. Therefore, it is necessary to sum up the latest results in time. This paper systematically depicted the profile of TCM and the importance of intestinal flora in host. What is more, we comprehensively summarized and discussed the latest progress of the interplay between TCM and intestinal flora to better reveal the core connotation of TCM.

Download Full-text