scholarly journals Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Sihan Li ◽  
Youcheng He ◽  
Haiou Zhang ◽  
Rong Zheng ◽  
Ruoying Xu ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Symbiotic Relationships ◽  
Study Results ◽  
Rrna Sequencing ◽  
Pcr Technology

AbstractIn this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the intestinal flora. We found that the relative abundance of Firmicutes with constipation was significantly higher accounted for 86.7%, while the gut microbiota was significantly changed after taking a certain dose of Chinese medicine, greatly increased the relative abundance of Lactobacillus accounted for 23.1%, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.

scholarly journals Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

2020 ◽  
Author(s):  
Minghan Huang ◽  
Sihan Li ◽  
Youcheng He ◽  
Haiou Zhang ◽  
Ruoying Xu ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Control Group ◽  
Symbiotic Relationships ◽  
Study Results ◽  
Significant Difference ◽  
Abundance And Diversity

Abstract In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the abundance and diversity of intestinal flora in rats. We found that the relative abundance of Firmicutes in the intestinal flora of rats with constipation was significantly higher than that in the control group, and the Bacteroides was significantly lower than that in the control group, while the relative abundance of Firmicutes was significantly changed after taking a certain dose of Chinese medicine, and reduced the relative abundance of Lachnospiraceae, and greatly increased the relative abundance of Lactobacillus, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. There was no significant difference in the total copies of intestinal bacteria between the constipated rats and the control group. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.

scholarly journals Effect of a Traditional Chinese Medicine Formula (CoTOL) on Serum Uric Acid and Intestinal Flora in Obese Hyperuricemic Mice Inoculated with Intestinal Bacteria

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Yan Gao ◽  
Jing Sun ◽  
Yi Zhang ◽  
Tiejuan Shao ◽  
Haichang Li ◽  
...  
Keyword(s):  
Uric Acid ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Mice Model ◽  
Blank Group ◽  
Gene Target ◽  
Streptococcus Faecalis ◽  
Beneficial Effects

CoTOL is a traditional Chinese medicine (TCM) formula in clinics for treating gout and hyperuricemia, especially in obese patients with recurrent attacks. However, fewer studies have investigated how CoTOL impacts the intestinal flora in reducing uric acid. In the present, we analyze the bacteria targeted by ingredients of CoTOL and evaluate the effects of CoTOL on uric acid and intestinal flora in a mice model of obese hyperuricemia inoculated with xanthine dehydrogenase- (XOD-) producing bacteria, Streptococcus faecalis. Firstly, ingredients of herbs in CoTOL and gene target by these ingredients were retrieved from TCMID 2.0, and these genes were screened by DAVID Bioinformatics Resources 6.8, deciphered to retrieve the bacteria. Then, 3-4-week-old male C57bl/6j mice were randomly divided into 6 groups and fed with high fat diet for 8 weeks up to obesity standard. The mice were inoculated intragastrically with 5 × 109 CFU Streptococcus faecalis 3 times at the 5th, 6th, and 7th week and intragastrically administrated with uricase inhibitor, potassium-oxonate (PO, 250 mg/kg), to induce hyperuricemia at the 8th week, once a day for 7 consecutive days, respectively (IB model). IB model plus CoTOL (0.4 ml/20g) and allopurinol (40 mg/kg) were administrated by gavage at the 5th week, once a day for 4 weeks. The feces and blood in each group were sampled at the 4th and 8th week. With no bacteria inoculation, CoTOL, allopurinol, and blank group were treated with CoTOL and allopurinol or water, respectively. 44 species of bacteria (i.e., Enterococcus faecalis, Streptococcus, etc.) genes were targeted by 6 ingredients of 6 herbs in CoTOL. Inoculation with Streptococcus faecalis significantly caused the elevation of uric acid and the change of intestinal flora structure, whereas treatment with CoTOL significantly increased the abundance of Akkermansia and those of Bacteroides and Alloprevotella decreased. Furthermore, CoTOL exhibited a unique effect on reducing weight unobserved in allopurinol intervention. The present study, for the first time, demonstrated that CoTOL has beneficial effects on hyperuricemia and overweight, which may be attributed to regulating material metabolism and improving the structure or function of intestinal flora. Thus, CoTOL may be a promising therapy for hyperuricemia and overweight in chronic gout management and can be integrated with conventional treatments.

scholarly journals Nigella A ameliorates inflammation and intestinal flora imbalance in DSS induced colitis mice

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xingjiang Hu ◽  
Nana Xu ◽  
Xi Yang ◽  
Xi Hu ◽  
Yunliang Zheng ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Inflammatory Response ◽  
Therapeutic Effect ◽  
Antioxidant Activities ◽  
Intestinal Flora ◽  
Signs And Symptoms ◽  
Microbial Composition ◽  
Bacterial Genus

Abstract Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and Sulfasalazine (SASP) could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.

The impact of Traditional Chinese Medicine on mitophagy in disease models

2021 ◽  
Vol 27 ◽  
Author(s):  
Li-Ping Yu ◽  
Ting-Ting Shi ◽  
Yan-Qin Li ◽  
Jian-Kang Mu ◽  
Ya-Qin Yang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Molecular Mechanisms ◽  
Human Diseases ◽  
Regulatory Mechanisms ◽  
Disease Models ◽  
Relationship Of ◽  
The Impact ◽  
The Relationship

: Mitophagy plays an important role in maintaining mitochondrial quality and cell homeostasis through the degradation of damaged, aged, and dysfunctional mitochondria and misfolded proteins. Many human diseases, particularly neurodegenerative diseases, are related to disorders of mitochondrial phagocytosis. Exploring the regulatory mechanisms of mitophagy is of great significance for revealing the molecular mechanisms underlying the related diseases. Herein, we summarize the major mechanisms of mitophagy, the relationship of mitophagy with human diseases, and the role of traditional Chinese medicine (TCM) in mitophagy. These discussions enhance our knowledge of mitophagy and its potential therapeutic targets using TCM.

scholarly journals Zhang Hongxing’s Experience in Exogenous Cough Treatment

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Renjie Zhou ◽  
Hongxing Zhang
Keyword(s):  
Clinical Practice ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Clinical Symptom ◽  
Clinical Diagnosis ◽  
Diagnosis And Treatment ◽  
Chief Physician

Cough is a common clinical symptom, throughout history the medical experts have different discussions on the diagnosis and treatment of cough and put forward different theories on the treatment of cough. Chief physician Zhang Hongxing is a famous old doctor of traditional Chinese medicine in Dezhou city with rich experience in clinical practice and unique academic thoughts. In the treatment of exogenous cough, Director Zhang stressed that the differentiation of syndromes should be focused on ‘wind’ and pay attention to the role of liver ‘wind’ in cough. The prescription of medicines should emphasize on dispelling the ‘wind’ first, to dispel the external ‘wind’, but also to calm the internal ‘wind’, and making good use of Uncaria in medicine. Valuable experience for clinical diagnosis and treatment of exogenous cough was provided.  

The role of the main representatives of the anaerobic intestinal flora in health and disease

2001 ◽  
Vol 82 (2) ◽  
pp. 149-151
Author(s):  
V. A. Anokhin ◽  
U. A. Tyurin
Keyword(s):  
Clinical Significance ◽  
Intestinal Microflora ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Obligate Anaerobic ◽  
Health And Disease ◽  
Normal Human

Normal intestinal microflora includes tens and hundreds of species, and their total number in an adult reaches 1014 microorganisms per 1 g of feces [7]. The basis of normal human microflora are obligate-anaerobic bifidobacteria, lactobacilli and bacteroids, the number of which is several orders of magnitude higher than the content of aerobic intestinal bacteria. In recent years, representatives of other anaerobic groups - Anaerovibrio, Butyrivibrio - have been found in the normal intestinal microflora, the biological and clinical significance of which is under study [7].

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