scholarly journals Liquid-Assisted Electrospinning Three-Dimensional Polyacrylonitrile Nanofiber Crosslinked with Chitosan

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Xiaoli Yang ◽  
Xue Chen ◽  
Jingyi Zhao ◽  
Wenlu Lv ◽  
Qilu Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Pore Structure ◽  
Antibacterial Effect ◽  
Fiber Diameter ◽  
Three Dimensional ◽  
Special Structure ◽  
Tissue Engineering Scaffolds ◽  
Antimicrobial Effectiveness ◽  
Pan Nanofiber

Electrospinning has become a popular nanotechnology for the fabrication of tissue engineering scaffolds, which can precisely regulate fiber diameter and microstructure. Herein, we have prepared a three-dimensional polyacrylonitrile (PAN) nanofiber by liquid-assisted electrospinning. The spacing between PAN nanofibers can reach to 15-20 μm, as the uniform internally connected pore structure can be formed, through the regulation of parameters. Furthermore, the chitosan attached to the as-prepared nanofibers gives the material antibacterial effect and increases its biocompatibility. Meanwhile, the special structure of chitosan also provides the possibility for further loading drugs in dressings in the future. This newly developed nanocomposite seems to be highly suitable for wound healing due to its unique properties of biodegradability, biocompatibility, and antimicrobial effectiveness.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

Preparation of Fibrous Scaffolds Containing Calcium and Silicon Species

2011 ◽  
Vol 493-494 ◽  
pp. 840-843
Author(s):  
Akiko Obata ◽  
Hiroki Ozasa ◽  
Julian R. Jones ◽  
Toshihiro Kasuga
Keyword(s):  
Tissue Engineering ◽  
Hybrid Materials ◽  
Fiber Diameter ◽  
Fiber Spinning ◽  
High Porosity ◽  
Cotton Wool ◽  
Tissue Engineering Scaffolds ◽  
Large Defect ◽  
Nanofibrous Structure ◽  
Versatile Technique

Materials for bone defect filling should have 3D macroporous structure and be flexible to be packed into complex defects with limited entrance space. Tissue engineering scaffolds should also mimic the structure and morphology of the host tissue. Electrospinning is a versatile technique to produce materials with micro/nanofibrous structure, large surface area and high porosity. Electrospun materials are very promising for tissue engineering due to the possibility of mimicking the fibrous structure of natural extra cellular matrix (ECM). Siloxane-containing vaterite (SiV)/poly (L-lactic acid) (PLLA) hybrids (SiPVH) with controlled silicate and calcium ions releasing ability has been produced in our group. They have also demonstrated good cell infiltration into the electrospun hybrid materials that had fiber diameters greater than 10 μm. However, these electrospun hybrid materials were planar (2D) and are not suitable for large defect regeneration. In this work, the development of a fabrication technique for the production of 3D cotton wool-like structures with fiber diameter in the range of 10 μm was performed. SiPVH cotton wool-like structure containing 0, 30 and 60 wt % SiV were prepared by blowing air in the direction perpendicular to fiber spinning. Si-vaterite particles and small pores were found on the surface of the fibers. The fiber diameter of the samples were found to be in the range of 10 ~ 20 μm. Stretch tests showed more than 50 % extension for the SiPVH cotton wool-like material containing 30 wt % SiV (SiPVH30). This extension was similar to that observed for the PLLA cotton wool-like material. The results suggest that the SiPVH30 cotton wool-like material are good candidates for bone tissue engineering scaffolds.

scholarly journals Three-Dimensional Microfluidic Tissue-Engineering Scaffolds Using a Flexible Biodegradable Polymer

2006 ◽  
Vol 18 (2) ◽  
pp. 165-169 ◽  
Author(s):  
C. J. Bettinger ◽  
E. J. Weinberg ◽  
K. M. Kulig ◽  
J. P. Vacanti ◽  
Y. Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biodegradable Polymer ◽  
Three Dimensional ◽  
Tissue Engineering Scaffolds

Mineralized Nanofibers for Bone Tissue Engineering

2018 ◽  
pp. 461-475 ◽  
Author(s):  
Ozan Karaman
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Bone Grafts ◽  
Tissue Engineering Scaffolds ◽  
Promising Alternative ◽  
Biomimetic Scaffolds

The limitation of orthopedic fractures and large bone defects treatments has brought the focus on fabricating bone grafts that could enhance ostegenesis and vascularization in-vitro. Developing biomimetic materials such as mineralized nanofibers that can provide three-dimensional templates of the natural bone extracellular-matrix is one of the most promising alternative for bone regeneration. Understanding the interactions between the structure of the scaffolds and cells and therefore the control cellular pathways are critical for developing functional bone grafts. In order to enhance bone regeneration, the engineered scaffold needs to mimic the characteristics of composite bone ECM. This chapter reviews the fabrication of and fabrication techniques for fabricating biomimetic bone tissue engineering scaffolds. In addition, the chapter covers design criteria for developing the scaffolds and examples of enhanced osteogenic differentiation outcomes by fabricating biomimetic scaffolds.

The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

2018 ◽  
Vol 934 ◽  
pp. 129-133 ◽  
Author(s):  
Chao Fan Lv ◽  
Li Ya Zhu ◽  
Jian Ping Shi ◽  
Zong An Li ◽  
Wen Lai Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Inkjet Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Tissue Engineering Scaffolds ◽  
Printing Technology ◽  
3D Printed ◽  
Inkjet Printing Technology ◽  
Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

scholarly journals Hybrid Tissue Engineering Scaffolds by Combination of Three-Dimensional Printing and Cell Photoencapsulation

2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Marica Markovic ◽  
Jasper Van Hoorick ◽  
Katja Hölzl ◽  
Maximilian Tromayer ◽  
Peter Gruber ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fused Deposition Modeling ◽  
Structural Parameters ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Tissue Engineering Scaffolds ◽  
Ecm Remodeling ◽  
Initial Cell ◽  
Fused Deposition ◽  
Hydrogel Matrix

Three-dimensional (3D) printing offers versatile possibilities for adapting the structural parameters of tissue engineering scaffolds. However, it is also essential to develop procedures allowing efficient cell seeding independent of scaffold geometry and pore size. The aim of this study was to establish a method for seeding the scaffolds using photopolymerizable cell-laden hydrogels. The latter facilitates convenient preparation, and handling of cell suspension, while distributing the hydrogel precursor throughout the pores, before it is cross-linked with light. In addition, encapsulation of living cells within hydrogels can produce constructs with high initial cell loading and intimate cell-matrix contact, similar to that of the natural extra-cellular matrix (ECM). Three dimensional scaffolds were produced from poly(lactic) acid (PLA) by means of fused deposition modeling. A solution of methacrylamide-modified gelatin (Gel-MOD) in cell culture medium containing photoinitiator Li-TPO-L was used as a hydrogel precursor. Being an enzymatically degradable derivative of natural collagen, gelatin-based matrices are biomimetic and potentially support the process of cell-induced remodeling. Preosteoblast cells MC3T3-E1 at a density of 10 × 106 cells per 1 mL were used for testing the seeding procedure and cell proliferation studies. Obtained results indicate that produced constructs support cell survival and proliferation over extended duration of our experiment. The established two-step approach for scaffold seeding with the cells is simple, rapid, and is shown to be highly reproducible. Furthermore, it enables precise control of the initial cell density, while yielding their uniform distribution throughout the scaffold. Such hybrid tissue engineering constructs merge the advantages of rigid 3D printed constructs with the soft hydrogel matrix, potentially mimicking the process of ECM remodeling.

scholarly journals A Nanofiber Mat With Dual Bioactive Components and a Biomimetic Matrix Structure for Improving Osteogenesis Effect

2021 ◽  
Vol 9 ◽  
Author(s):  
Yadi Han ◽  
Xiaofeng Shen ◽  
Sihao Chen ◽  
Xiuhui Wang ◽  
Juan Du ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bone Repair ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Bioactive Components ◽  
Tissue Engineering Scaffolds ◽  
Cell Growth And Migration ◽  
Nanofiber Mats

The challenge of effectively regenerating bone tissue through tissue engineering technology is that most tissue engineering scaffolds cannot imitate the three-dimensional structure and function of the natural extracellular matrix. Herein, we have prepared the poly(L-lactic acid)–based dual bioactive component reinforced nanofiber mats which were named as poly(L-lactic acid)/bovine serum albumin/nanohydroxyapatite (PLLA/BSA/nHAp) with dual bioactive components by combining homogeneous blending and electrospinning technology. The results showed that these nanofiber mats had sufficient mechanical properties and a porous structure suitable for cell growth and migration. Furthermore, the results of cell experiments in vitro showed that PLLA/BSA/nHAp composite nanofiber mat could preferably stimulate the proliferation of mouse osteoblastic cells (MC3T3 cells) compared with pure PLLA nanofiber mats. Based on these results, the scaffolds developed in this study are considered to have a great potential to be adhibited as bone repair materials.

scholarly journals Antibacterial cellulose-based aerogels for wound healing application: A review

2020 ◽  
Vol 7 (10) ◽  
pp. 4032-4040
Author(s):  
Esam Bashir Yahya ◽  
Marwa Mohammed Alzalouk ◽  
Khalifa A. Alfallous ◽  
Abdullah F. Abogmaza
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Wound Healing ◽  
Wound Dressing ◽  
Biomedical Applications ◽  
Inorganic Materials ◽  
Medical Applications ◽  
Tissue Engineering Scaffolds

Aerogels have been steadily developed since its first invention to become one of the most promising materials for various medical and non-medical applications. It has been prepared from organic and inorganic materials, in pure forms or composites. Cellulose-based aerogels are considered one of the promising materials in biomedical applications due to their availability, degradability, biocompatibility and non-cytotoxicity compared to conventional silica or metal-based aerogels. The unique properties of such materials permit their utilization in drug delivery, biosensing, tissue engineering scaffolds, and wound dressing. This review presents a summary of aerogel development as well as the properties and applications of aerogels. Herein, we further discuss the recent works pertaining to utilization of cellulose-based aerogels for antibacterial delivery.

Thermal Processing of Tissue Engineering Scaffolds

2010 ◽  
Vol 133 (3) ◽  
Author(s):  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Thermal Processing ◽  
Three Dimensional ◽  
Processing Parameters ◽  
High Pressure Processing ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Thermally Induced ◽  
Advantages And Disadvantages ◽  
Particulate Leaching

Tissue engineering requires complex three-dimensional scaffolds that mimic natural extracellular matrix function. A wide variety of techniques have been developed to create both fibrous and porous scaffolds out of polymers, ceramics, metals, and composite materials. Existing techniques include fiber bonding, electrospinning, emulsion freeze drying, solvent casting/particulate leaching, gas foaming/particulate leaching, high pressure processing, and thermally induced phase separation. Critical scaffold properties, including pore size, porosity, pore interconnectivity, and mechanical integrity, are determined by thermal processing parameters in many of these techniques. In this review, each tissue engineering scaffold preparation method is discussed, including recent advancements as well as advantages and disadvantages of the technique, with a particular emphasis placed on thermal parameters. Improvements on these existing techniques, as well as new thermal processing methods for tissue engineering scaffolds, will be needed to provide tissue engineers with finer control over tissue and organ development.

Sign in / Sign up

Export Citation Format

Share Document