scholarly journals Salvia spinosa L. Protects against Diabetes-Induced Nephropathy by Attenuation of Mitochondrial Oxidative Damage in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Milad Jeshan ◽  
Fatemeh Yousefbeyk ◽  
Hiva Rahmati ◽  
Amir Hosein Shoormeij ◽  
Mitra Rezazadeh ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Mitochondrial Function ◽  
Weight Coefficient ◽  
Diabetic Control ◽  
Histological Evaluation ◽  
Control Group ◽  
Diabetic Mice ◽  
Promising Alternative ◽  
Oxidative Markers ◽  
Mitochondrial Oxidative Damage

Mitochondrial oxidative damage is a crucial factor in the pathogenesis of diabetic nephropathy (DN), which is among the most prevalent problems of diabetes, and there hasn’t been an effective treatment for DN yet. This study planned to investigate the effects of Salvia spinosa L. on mitochondrial function along with its protection against streptozotocin-induced nephropathy in diabetic mice. After the injection of streptozotocin (STZ) and verification of the establishment of diabetes, mice (n = 30) were randomly divided into the following groups: control group, diabetic-control, S. spinosa-treated diabetic (50, 100, and 200 mg/kg), and metformin-treated diabetic group (500 mg/kg). After four weeks of treatment, the mice were weighed. Blood and kidney tissues were examined for biochemical and histological evaluation. Hematoxylin and eosin staining was used for evaluating renal pathologic damage. Oxidative damage in the kidney was assessed by the evaluation of lipid peroxidation and glutathione oxidation. Furthermore, differential centrifugation was used to obtain the isolated mitochondria, and mitochondrial toxicity endpoints (mitochondrial function and mitochondrial oxidative markers) were determined in them. S. spinosa remarkably reduced the blood urea and creatinine concentrations, and also normalized kidney weight/body weight coefficient in the diabetic mice. S. spinosa ameliorated the incidence of glomerular and tubular pathological changes in histological analyses. Moreover, the oxidative and mitochondrial damages were notably attenuated in renal tissues of S. spinosa-treated mice. These results indicate that the methanolic extract of S. spinosa modulates the nephropathy in the diabetic mice by the amelioration of oxidatively induced mitochondrial damage and provides a reliable scientific base, suggesting S. spinosa as a promising alternative remedy against DN.

scholarly journals Evaluation of solubility and histological effect of 11-keto-β-Boswellic acid on the diabetic mice liver using FTIR-PCA analysis and microscopy

2021 ◽  
Author(s):  
I.S. Al-Amri ◽  
F. Mabood ◽  
I.T. Kadim ◽  
A.Y. Alkindi ◽  
A. Al-Harrasi ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Liver Tissue ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Mice ◽  
Boswellic Acid ◽  
Ft Ir ◽  
Mice Liver ◽  
Ft Ir Spectroscopy

ABSTRACTThis study was designed to develop a rapid, sensitive, accurate, and inexpensive Fourier Transform Infrared Reflectance (FT-IR) Spectroscopy coupled with Principle Component Analysis (PCA) as a detection technique to evaluate the solubility of 11-Keto-β-Boswellic acid (KBA), from the gum resin extracted from the Omani frankincense, (Boswellia sacra) in the liver of STZ induced diabetic mice. This study also investigated the effect of KBA on the histological changes of hepatocytes of diabetic mice. Liver tissue samples from three groups of mice included normal control group, diabetic control group and diabetic group treated IP with KBA were scanned with FT-IR spectrophotometer in the reflection mode. FT-IR Spectra were collected in the wavenumber range from 400 to 4000cm-1 using ATR accessorry. The results of FT-IR Spectra were analyzed by using multivariate method Principle Component Analysis. The PCA score plot is an exploratory multivariate method indicated that there was a complete segregation among the three groups of liver samples based on change in variation of position of wavenumber in FT-IR spectra, which revealed that there is a clear effect of KBA solubility on treatments. The histological features showed an improvement in the liver tissues with normal structures of hepatocytes with exhibiting mild vacuolations in their cytoplasm. In conclusion, reflectance FT-IR spectroscopy coupled with PCA could be deployed as a new detection method for rapid, low cost and non-destructive method for evaluating of treatment effects in diseased liver tissue based on the solubility of KBA. Histological findings demonstrated the protective effective of KBA on improving the morphology of liver tissue in diabetic mice which resulted in complete recovery to the damage observed in diabetic control group.Summary StatementReflectance FT-IR spectroscopy coupled with PCA has been deployed as a new rapid, inexpensive and non-destructive detection method to examine the solubility of 11-keto-β-Boswellic acid (KBA) in streptozotocin (STZ) induced-diabetes mice liver tissue following intraperitoneal treatment. Moreover, microscopic study of liver tissue histopathology revealed that KBA has a protecting effect against STZ damage.

scholarly journals Methoxsalen and Bergapten Prevent Diabetes-Induced Osteoporosis by the Suppression of Osteoclastogenic Gene Expression in Mice

2019 ◽  
Vol 20 (6) ◽  
pp. 1298 ◽  
Author(s):  
Ju Ham ◽  
Ra-Yeong Choi ◽  
Hae-In Lee ◽  
Mi-Kyung Lee
Keyword(s):  
Underlying Mechanism ◽  
Volume Density ◽  
Diabetic Control ◽  
Bone Alkaline Phosphatase ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Diabetic Mice ◽  
Mineral Density ◽  
Activated T Cells ◽  
Diabetic Osteoporosis

This study evaluated whether bergapten and methoxsalen could prevent diabetes-induced osteoporosis and its underlying mechanism. For 10 weeks, bergapten or methoxsalen (0.02%, w/w) was applied to diabetic mice that were provided with a high-fat diet and streptozotocin. Bone mineral density (BMD) and microarchitecture quality were significantly reduced in the diabetic control group; however, both bergapten and methoxsalen reversed serum osteocalcin, bone-alkaline phosphatase and femur BMD. These coumarin derivatives significantly increased bone volume density and trabecular number, whereas they decreased the structure model index of femur tissue in diabetic mice. Conversely, tartrate-resistant acid phosphatase 5 (TRAP) staining revealed that these derivatives reduced osteoclast numbers and formation in diabetic bone tissue. Additionally, both bergapten and methoxsalen tended to downregulate the expression of osteoclast-related genes such as receptor activator of nuclear factor kappa-B ligand (RANKL), nuclear of activated T-cells, cytoplasmic 1 (NFATc1) and TRAP in diabetic femurs, with NFATc1 and TRAP expression showing significant reductions. Our data suggest that both bergapten and methoxsalen prevent diabetic osteoporosis by suppressing bone resorption.

scholarly journals Myricitrin Ameliorates Hyperglycemia, Glucose Intolerance, Hepatic Steatosis, and Inflammation in High-Fat Diet/Streptozotocin-Induced Diabetic Mice

2020 ◽  
Vol 21 (5) ◽  
pp. 1870
Author(s):  
Do Yeon Kim ◽  
Sang Ryong Kim ◽  
Un Ju Jung
Keyword(s):  
Mrna Expression ◽  
Hepatic Steatosis ◽  
Glucose Intolerance ◽  
High Fat Diet ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Mice ◽  
High Fat ◽  
Expression And Activity

To test the hypothesis that myricitrin (MYR) improves type 2 diabetes, we examined the effect of MYR on hyperglycemia, glucose intolerance, hepatic steatosis, and inflammation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice. Male C57BL/6J mice were randomly divided into three groups: non-diabetic, diabetic control, and MYR (0.005%, w/w)-supplemented diabetic groups. Diabetes was induced by HFD and STZ, and MYR was administered orally for 5 weeks. Myricitrin exerted no significant effects on food intake, body weight, fat weight, or plasma lipids levels. However, MYR significantly decreased fasting blood glucose levels, improved glucose intolerance, and increased pancreatic β-cell mass compared to the diabetic control group. Myricitrin administration also markedly increased glucokinase mRNA expression and activity as well as lowered glucose-6-phosphatase and phosphoenolpyruvate carboxykinase mRNA expression and activity in the liver. In addition, liver weight, hepatic triglyceride content, and lipid droplet accumulation were markedly decreased following MYR administration. These changes were seemingly attributable to the suppression of the hepatic lipogenic enzymes—fatty acid synthase and phosphatidate phosphohydrolase. Myricitrin also significantly lowered plasma MCP-1 and TNF-α levels and the mRNA expression of hepatic pro-inflammatory genes. These results suggest that MYR has anti-diabetic potential.

scholarly journals Evaluation of the solubility of 11-keto-β-boswellic acid and its histological effect on the diabetic mice liver using a novel technique

2021 ◽  
pp. 1797-1803
Author(s):  
Issa Al Amri ◽  
Fazal Mabood ◽  
Isam T. Kadim ◽  
Abdulaziz Alkindi ◽  
A. Al-Harrasi ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Diabetic Control ◽  
Reflectance Spectroscopy ◽  
Reflectance Spectra ◽  
Control Group ◽  
Diabetic Mice ◽  
Boswellic Acid ◽  
Data Set ◽  
Diabetic Control Group ◽  
Liver Tissues

Background and Aim: The literature is scant on the effect of 11-keto-β-boswellic acid (KBA) on the liver of diabetes-induced mice. This study was designed to develop a rapid, sensitive, accurate, and inexpensive detection technique for evaluating the solubility of KBA obtained from the gum resin of Omani frankincense (Boswellia sacra) in the liver of streptozotocin-induced diabetic mice using Fourier transform infrared (FTIR) reflectance spectroscopy coupled with principal components analysis (PCA). It also aimed to investigate the effect of KBA on histological changes in the hepatocytes of diabetic mice. Materials and Methods: Eighteen mice were assigned to the healthy control group, the diabetic control group, or the KBA-treated diabetic group. Liver tissue samples from all groups were scanned using an FTIR reflectance spectrophotometer in reflection mode. FTIR reflectance spectra were collected in the wavenumber range of 400-4000 cm-1 using an attenuated total reflectance apparatus. Results: FTIR reflectance spectra were analyzed using PCA. The PCA score plot, which is an exploratory multivariate data set, revealed complete segregation among the three groups' liver samples based on changes in the variation of wavenumber position in the FTIR reflectance spectra, which indicated a clear effect of KBA solubility on treatments. Histological analysis showed an improvement in the liver tissues, with normal structures of hepatocytes exhibiting mild vacuolation in their cytoplasm. Conclusion: KBA improved the morphology of liver tissues in the diabetic mice and led to complete recovery of the damage observed in the diabetic control group. FTIR reflectance spectroscopy coupled with PCA could be deployed as a rapid, low-cost, and non-destructive detection method for evaluating treatment effects in diseased liver tissue based on the solubility of KBA.

scholarly journals Potential Antidiabetic Activity of Extracts and Isolated Compound from Adenosma bracteosum (Bonati)

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 201 ◽  
Author(s):  
Ngoc Hong Nguyen ◽  
Quang Thang Pham ◽  
Thi Ngoc Han Luong ◽  
Hoang Khai Le ◽  
Van Giau Vo
Keyword(s):  
Inhibitory Activity ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Positive Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Aqueous Extracts ◽  
Diabetic Mice ◽  
Mice Model ◽  
Standard Drug

Adenosma bracteosum Bonati. (A. bracteosum) has been used in traditional and modern medicine in Vietnam for curing hepatitis. In this study, ethanol and aqueous extracts of A. bracteosum were evaluated for their α-glucosidase inhibitory activities and anti-hyperglycemic effects on glucose loaded hyperglycemic and streptozotocin (STZ) induced diabetic mice. The α-glucosidase inhibition of the extracts was evaluated by colorimetric assays, and the anti-diabetic activity was tested on a STZ-induced diabetic mice model. The ethanol and aqueous extracts showed a significant α-glucosidase inhibitory activity, which was more effective than acarbose at the same concentration. In the STZ-induced diabetic mice, both extracts showed a strong anti-hyperglycemic activity, with the group receiving 50 mg/kg of ethanol extract and the group receiving 50 mg/kg of aqueous extract presenting 64.42% and 57.69% reductions, respectively, in the blood glucose levels when compared with the diabetic control group, on day 21 (p > 0.05). Isoscutellarein-8-O-β-D-glucopyranoside (IG) was identified from the ethanol extract, which showed a strong inhibitory activity against α-glucosidase, with a ten times higher potency compared with the positive control acarbose. The anti-hyperglycemic effect of IG was effectively similar to the standard drug, glibenclamide, at the same dose of 10 mg/kg (p > 0.05). These results indicated that A. bracteosum has a great antidiabetic potential.

scholarly journals Immunomodulatory and Antidiabetic Effects of a New Herbal Preparation (HemoHIM) on Streptozotocin-Induced Diabetic Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jong-Jin Kim ◽  
Jina Choi ◽  
Mi-Kyung Lee ◽  
Kyung-Yun Kang ◽  
Man-Jeong Paik ◽  
...  
Keyword(s):  
White Blood Cells ◽  
Diabetic Control ◽  
Control Group ◽  
Herbal Preparation ◽  
Diabetic Mice ◽  
Angelica Gigas ◽  
Diabetic Control Group ◽  
Plasma Insulin Levels ◽  
Insulin Staining ◽  
Lymphocyte Populations

HemoHIM (a new herbal preparation of three edible herbs:Angelica gigasNakai,Cnidium officinaleMakino, andPaeonia japonicaMiyabe) was developed to protect immune, hematopoietic, and self-renewal tissues against radiation. This study determined whether or not HemoHIM could alter hyperglycemia and the immune response in diabetic mice. Both nondiabetic and diabetic mice were orally administered HemoHIM (100 mg/kg) once a day for 4 weeks. Diabetes was induced by single injection of streptozotocin (STZ, 200 mg/kg, i.p.). In diabetic mice, HemoHIM effectively improved hyperglycemia and glucose tolerance compared to the diabetic control group as well as elevated plasma insulin levels with preservation of insulin staining in pancreaticβ-cells. HemoHIM treatment restored thymus weight, white blood cells, lymphocyte numbers, and splenic lymphocyte populations (CD4+T and CD8+T), which were reduced in diabetic mice, as well as IFN-γproduction in response to Con A stimulation. These results indicate that HemoHIM may have potential as a glucose-lowering and immunomodulatory agent by enhancing the immune function of pancreaticβ-cells in STZ-induced diabetic mice.

scholarly journals Ameliorative Effect of Chlorophyllin on Oxidative Stress in Experimental Model of Diabetes

2017 ◽  
Vol 8 (4) ◽  
pp. 506 ◽  
Author(s):  
Abani K Patar ◽  
Surya Bhan ◽  
Donkupar Syiem ◽  
Anupama Sharma
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Diabetic Control ◽  
Protein Carbonyl ◽  
Control Group ◽  
Diabetic Mice ◽  
Ultrastructural Studies ◽  
Ameliorative Effect ◽  
Group A ◽  
Group B

The aim of this present study was to investigate the effect of chlorophyllin (CHL) on oxidative stress in Streptozotocine (STZ) induced diabetic mice. For the study, mice were divided into Group A: normal control, Group B: diabetic control, Group C: diabetic mice treated with the ascorbic acid, and Group D: diabetic mice treated with CHL. Levels of Reactive Oxygen Species (ROS), lipid peroxidation, protein carbonyl, superoxide dismutase (CuZn SOD &Mn-SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were examined in kidney and heart tissues of different experimental groups. Histological and ultrastructuralstudies were also carried out to evaluate any changes in tissues as well as sub-cellular organelles. ROS, lipid peroxidation, and protein carbonyl levels have been significantly decreased with concomitant increased of CuZn SOD, Mn-SOD, CAT, GPx, and GR activity in CHLtreated diabetic mice. The histological and ultrastructural studies showed that CHL attenuates the detrimental effect of oxidative stress and alleviated tissue injuries in STZ induced diabetic mice. These results suggested that CHL possesses antioxidative activity and has the potential to amelioratediabetes-associated oxidative stress in mice.

scholarly journals Antidiabetic and Wound Healing Effects of Smeathxanthone A

2016 ◽  
Vol 02 ◽  
pp. 5 ◽  
Author(s):  
David Emery Tsala ◽  
Alain Meli Lannang ◽  
Theophile Dimo ◽  
Solomon Habtemariam ◽  
Jean Arnaud Ekanga ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Breaking Strength ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Tissue Healing ◽  
Diabetic Control Group

The purpose of this study was to investigate whether smeathxanthone A isolated from Garcinia smeathmaniiimproves incisional wound healing in diabetic mice. Male albino alloxan-induced diabetic mice (n= 20) were divided into five groups: normal control, diabetic control, 2.5 mg/kg glibenclamide given orally, 0.05 and 0.1 mg/kg smeathxanthone A given subcutaneously. Animals were euthanized on postoperative day 10 after wounding; body weight, blood glucose, breaking strength, and histologic examination were reviewed. Smeathxanthone A significantly increased skin tensile strength (24% higher than diabetic control group when given at 0.1 mg/kg), stimulated hair growth, and reduced signs of inflammation in the scar sections. Smeathxanthone A also reduced blood glucose levels in diabetic mice (45% higher than diabetic control group when given at 0.1 mg/kg). The present study demonstrates that administration of smeathxanthone A after laparotomy expedites wound healing in mice. We suggest that it could confer benefits to tissue healing by significantly enhancing tissue collagen deposition and controlling blood glucose levels.

scholarly journals Effect of Alcoholic Catechin Extract on Hyperglycemia, Hyperlipidemia and Liver Functions in Alloxan Diabetic Mice

2014 ◽  
Vol 11 (3) ◽  
pp. 1192-1200
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Glucose ◽  
Diabetic Control ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Diabetic Mice ◽  
Diabetic Control Group ◽  
Liver Functions ◽  
Group A ◽  
Serum Biochemical ◽  
Group B

The objective of this study is to estimate the effect of the hydro-ethanolic catechin extract toward blood glucose, lipid profile and liver functions in alloxan diabetic mice. 50 healthy mice (25-30 g) were divided into five groups of ten animals for each. Group A received normal saline as normal control group. To induce diabetes, alloxan (150 mg/kg), intraperitoneal (i.p.) single dose was injected to groups B, C, D and E. Group B represents diabetic control group. Groups C, D and E received ethanolic catechin extract (30 mg/kg and 40 mg/kg) for different periods of 1, 2 and 3 weeks as treated groups. Blood glucose, serum lipids [Total Cholesterol (TC), Triglycerides (TGs) and High Density Lipoproteins (HDL)], asparagine transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were estimated after one, two and three weeks. Group B showed a significant increase in blood glucose, TC, TGs, AST, ALT and ALP as compared to group A. Groups C, D, and E showed a significant decrease in mentioned serum biochemical parameters in comparison to group B. In contrast, groups C, D and E showed significant increase in serum HDL as compared to B group. The results clearly revealed that ethanolic catechin extract possesses significant antihyperglycemic and antihyperlipidemic activities together with its ability to improve liver functions in alloxan diabetic mice.

Camel milk ameliorates hyperglycaemia and oxidative damage in type-1 diabetic experimental rats

2016 ◽  
Vol 83 (3) ◽  
pp. 412-419 ◽  
Author(s):  
Sunita Meena ◽  
Yudhishthir S Rajput ◽  
Amit K Pandey ◽  
Rajan Sharma ◽  
Raghvendar Singh
Keyword(s):  
Oxidative Damage ◽  
Density Lipoprotein ◽  
Insulin Level ◽  
Diabetic Control ◽  
Buffalo Milk ◽  
Diabetic Rats ◽  
Camel Milk ◽  
Control Group ◽  
Kidney Liver

This study was designed to assess anti-diabetic potential of goat, camel, cow and buffalo milk in streptozotocin (STZ) induced type 1 diabetic albino wistar rats. A total of 48 rats were taken for the study where one group was kept as non-diabetic control group (8 rats) while others (40 rats) were made diabetic by STZ (50 mg/kg of body weight) injection. Among diabetic rats, a control group (8 rats) was kept and referred as diabetic control whereas other four groups (8 rats each) of diabetic rats were fed on 50 ml of goat or camel or cow or buffalo milk for 4 weeks. All the rats (non-diabetic and diabetic) were maintained on standard diet for four weeks. STZ administration resulted in enhancement of glucose, total cholesterol, triglyceride, low density lipoprotein, HbA1cand reduction in high density lipoprotein in plasma and lowering of antioxidative enzymes (catalase, glutathione peroxidase and superoxide dismutase) activities in pancreas, kidney, liver and RBCs, coupled with enhanced levels of TBARS and protein carbonyls in pancreas, kidney, liver and plasma. OGTT carried out at the end of 4 week milk feeding indicated that all milks helped in early maintenance of glucose level. All milks reduced atherogenic index. In camel milk fed diabetic group, insulin concentration enhanced to level noted for non-diabetic control while goat, cow and buffalo milk failed to restore insulin level. HbA1clevel was also restored only in camel milk fed diabetic group. The level of antioxidative enzymes (catalase, GPx and SOD) in pancreas enhanced in all milk fed groups. Camel milk and to a reasonable extent goat milk reduced formation of TBARS and PCs in tissues and blood. It can be concluded that camel milk ameliorates hyperglycaemia and oxidative damage in type-1 diabetic experimental rats. Further, only camel milk completely ameliorated oxidative damage in pancreas and normalised insulin level.

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