scholarly journals Analysis of Bioactive Components in the Fruit, Roots, and Leaves of Alpinia oxyphylla by UPLC-MS/MS

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Li Ying ◽  
Deli Wang ◽  
Guankui Du
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Acids ◽  
Economic Value ◽  
Network Pharmacology ◽  
Bioactive Components ◽  
Comprehensive Utilization ◽  
Alpinia Oxyphylla ◽  
Medicinal Value ◽  
Whole Plant ◽  
Disease Related Genes

Alpinia oxyphylla (A. oxyphylla) fruit has long been used in traditional Chinese medicine. In our study, the bioactive components of its roots, fruit, and leaves were investigated, and their potential medical value was predicted. The root, fruit, and leaf samples were analyzed using a UPLC-MS/MS system. The mass spectrometry outcomes were annotated by MULTIAQUANT. The “compound-disease targets” were used to construct a pharmacology network. A total of 293, 277, and 251 components were identified in the roots, fruit, and leaves, respectively. The fruit of A. oxyphylla had a higher abundance of flavonols. The roots of A. oxyphylla were enriched in flavonols and phenolic acids. The leaves of A. oxyphylla exhibited high contents of flavonols, phenolic acids, and tannins. Furthermore, network pharmacology analysis showed that flavonoids are the most important effectors in the fruit of A. oxyphylla and phenolic acids are the most important effectors in the roots and leaves. Moreover, the results suggested that the tissues of A. oxyphylla might play a role in the regulation of disease-related genes. The whole plant of A. oxyphylla is rich in natural drug components, and each tissue has high medicinal value. Therefore, comprehensive utilization of A. oxyphylla can greatly improve its economic value.

scholarly journals Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhichen Cai ◽  
Xunhong Liu ◽  
Huan Chen ◽  
Rong Yang ◽  
Jiajia Chen ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Salt Stress ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Vital Role ◽  
Gray Relational Analysis ◽  
Bioactive Constituents ◽  
Multivariate Statistical ◽  
Medicinal Value ◽  
Quality Formation

AbstractLonicerae Japonicae Flos (LJF) is an important traditional Chinese medicine for the treatment of various ailments and plays a vital role in improving global human health. However, as unable to escape from adversity, the quality of sessile organisms is dramatically affected by salt stress. To systematically explore the quality formation of LJF in morphology, physiology, and bioactive constituents' response to multiple levels of salt stress, UFLC-QTRAP-MS/MS and multivariate statistical analysis were performed. Lonicera japonica Thunb. was planted in pots and placed in the field, then harvested after 35 days under salt stress. Indexes of growth, photosynthetic pigments, osmolytes, lipid peroxidation, and antioxidant enzymes were identified to evaluate the salt tolerance in LJF under different salt stresses (0, 100, 200, and 300 mM NaCl). Then, the total accumulation and dynamic variation of 47 bioactive constituents were quantitated. Finally, Partial least squares discrimination analysis and gray relational analysis were performed to systematically cluster, distinguish, and evaluate the samples, respectively. The results showed that 100 mM NaCl induced growth, photosynthetic, antioxidant activities, osmolytes, lipid peroxidation, and multiple bioactive constituents in LJF, which possessed the best quality. Additionally, a positive correlation was found between the accumulation of phenolic acids with antioxidant enzyme activity under salt stress, further confirming that phenolic acids could reduce oxidative damage. This study provides insight into the quality formation and valuable information to improve the LJF medicinal value under salt stress.

scholarly journals Study on the Sleep-Improvement Effects of Hemerocallis citrina Baroni in Drosophila melanogaster and Targeted Screening to Identify Its Active Components and Mechanism

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 883
Author(s):  
Yuxuan Liang ◽  
Riming Huang ◽  
Yongchun Chen ◽  
Jing Zhong ◽  
Jie Deng ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
High Resolution Mass Spectrometry ◽  
Sleep Time ◽  
Network Pharmacology ◽  
Dependent Manner ◽  
Bioactive Components ◽  
Active Components ◽  
Edible Plant ◽  
Hemerocallis Citrina ◽  
Ultrahigh Performance Liquid Chromatography

Hemerocallis citrina Baroni (HC) is an edible plant in Asia, and it has been traditionally used for sleep-improvement. However, the bioactive components and mechanism of HC in sleep-improvement are still unclear. In this study, the sleep-improvement effect of HC hydroalcoholic extract was investigated based on a caffeine-induced insomnia model in Drosophila melanogaster (D. melanogaster), and the ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-ESI-Orbitrap-MS) and network pharmacology strategy were further combined to screen systematically the active constituents and mechanism of HC in sleep-improvement. The results suggested HC effectively regulated the number of nighttime activities and total sleep time of D. melanogaster in a dose-dependent manner and positively regulated the sleep bouts and sleep duration of D. melanogaster. The target screening suggested that quercetin, luteolin, kaempferol, caffeic acid, and nicotinic acid were the main bioactive components of HC in sleep-improvements. Moreover, the core targets (Akt1, Cat, Ple, and Sod) affected by HC were verified by the expression of the mRNA of D. melanogaster. In summary, this study showed that HC could effectively regulate the sleep of D. melanogaster and further clarifies the multi-component and multi-target features of HC in sleep-improvement, which provides a new insight for the research and utilization of HC.

scholarly journals Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3770
Author(s):  
Željka Peršurić ◽  
Sandra Kraljević Pavelić
Keyword(s):  
Mass Spectrometry ◽  
Wound Healing ◽  
Chemical Composition ◽  
Extracellular Vesicles ◽  
Royal Jelly ◽  
Geographical Origin ◽  
Bioactive Components ◽  
Animal Cells ◽  
Health Promoting ◽  
High Diversity

In recent years, interest has surged among researchers to determine compounds from bee products such as honey, royal jelly, propolis and bee pollen, which are beneficial to human health. Mass spectrometry techniques have shown that bee products contain a number of proven health-promoting compounds but also revealed rather high diversity in the chemical composition of bee products depending on several factors, such as for example botanical sources and geographical origin. In the present paper, we present recent scientific advances in the field of major bioactive compounds from bee products and corresponding regenerative properties. We also discuss extracellular vesicles from bee products as a potential novel bioactive nutraceutical component. Extracellular vesicles are cell-derived membranous structures that show promising potential in various therapeutic areas. It has been extensively reported that the use of vesicles, which are naturally formed in plant and animal cells, as delivery agents have many advantages. Whether the use of extracellular vesicles from bee products represents a new solution for wound healing remains still to be elucidated. However, promising results in specific applications of the bee products in wound healing and tissue regenerative properties of extracellular vesicles provide a good rationale to further explore this idea.

Chromatographic Methods Coupled to Mass Spectrometry Detection for the Determination of Phenolic Acids in Plants and Fruits

2014 ◽  
Vol 38 (3) ◽  
pp. 353-370 ◽  
Author(s):  
Anna Laura Capriotti ◽  
Chiara Cavaliere ◽  
Patrizia Foglia ◽  
Susy Piovesana ◽  
Salvatore Ventura
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Acids ◽  
Mass Spectrometry Detection ◽  
Chromatographic Methods

Molecular Mechanism of The Effect of Huanglian Jiedu Decoction On Ulcerative Colitis Based On Network Pharmacology And Molecular Docking

2021 ◽  
Author(s):  
Jing Yang ◽  
Chao-Tao Tang ◽  
Ruiri Jin ◽  
Bixia Liu ◽  
Peng Wang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Molecular Docking ◽  
Molecular Mechanism ◽  
Target Genes ◽  
Signal Pathway ◽  
Intestinal Barrier Function ◽  
Network Pharmacology ◽  
Bioactive Components ◽  
Ppi Network ◽  
Active Components

Abstract Huanglian jiedu decoction (HLJDD) is a heat-clearing and detoxifying agent composed of four kinds of Chinese herbal medicine. Previous studies have shown that HLJDD can improve the inflammatory response of ulcerative colitis (UC) and maintain intestinal barrier function. However, its molecular mechanism is not completely clear. In this study, we verified the bioactive components (BCI) and potential targets of HLJDD in the treatment of UC by means of network pharmacology and molecular docking, and constructed the pharmacological network and PPI network. Then the core genes were enriched by GO and KEGG. Finally, the bioactive components were docked with the key targets to verify the binding ability between them. A total of 54 active components related to UC were identified. Ten genes are considered to be very important to PPI network. Functional analysis showed that these target genes were mainly involved in the regulation of cell response to different stimuli, IL-17 signal pathway and TNF signal pathway. The results of molecular docking showed that the active components of HLJDD had good affinity with Hub gene. This study systematically elucidates the "multi-component, multi-target, multi-pathway" mechanism of anti-UC with HLJDD for the first time, suggesting that HLJDD or its active components may be candidate drugs for the treatment of ulcerative colitis.

scholarly journals A Network Pharmacology Approach to Predict the Proangiogenesis Mechanism of Huangqi-Honghua Herb Pair after Cerebral Ischemia

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Jinyi Cao ◽  
Lu Lei ◽  
Kai Wang ◽  
Jing Sun ◽  
Yi Qiao ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Signaling Pathway ◽  
Target Genes ◽  
Blood Stasis ◽  
Blood Stasis Syndrome ◽  
Network Pharmacology ◽  
Western Blot Assay ◽  
Medicinal Value ◽  
Pharmacological Method

Objective. Huangqi-Honghua herb pair is known for its medicinal value to treat Qi deficiency and blood stasis syndrome with a long history in clinical practice. To understand its possible mechanism in a systematic study, a network pharmacological method was addressed. Methods. Detailed information on the HH compounds was obtained from two public databases, and oral bioavailability (OB) and drug-like (DL) of the compounds were evaluated. A correlation between HH compounds, its potential targets, and known targets was extrapolated, and the herb-compound-target-disease (H-C-T-D) network was established. Next, the pathway enrichment and essential genes were analyzed. Then, three key genes (VEGFA, VEGFR2, and eNOS), highly associated with angiogenesis, were screened and verified through western blot assay. Results. Out of 276 compounds, 21 HH compounds and 78 target genes regulating the major pathways associated with CI in the network are analyzed. The bioactive compounds in HH were active in various signal transduction pathways such as the toll-like receptor signaling pathway, VEGF signaling pathway, TNF signaling pathway, and HIF-1 signaling pathway are important pathways that may regulate anti-inflammatory, antiapoptotic, immune correlation, and antioxidative effects. The core genes are PTGS2, TNF, NOS2, IL6, BCL2, IL1B, SOD2, NOS3, SOD1, MMP9, and VEGFA. The in vitro results suggested that HH treatment could significantly elevate the expression of proangiogenic genes such as VEGFA, VEGFR2, and eNOS compared with OGD groups. Conclusions. Our results predict that HH may regulate the expression of VEGFA, VEGFR2, and eNOS via the VEGF and HIF-1 signaling pathway to promote angiogenesis and alleviate cerebral ischemia injury.

scholarly journals Chloroplast Genome Analysis of The Famous Chinese Herbal Medicine Yizhiren (Zingiberaceae)

2021 ◽  
Author(s):  
JiaoYi Pan ◽  
Sun Shiyun ◽  
Xia Qiong ◽  
Lv Xvhan ◽  
Yang Xinxian ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chloroplast Genome ◽  
Species Identification ◽  
Chinese Herbal Medicine ◽  
Gc Content ◽  
Single Copy ◽  
Likelihood Method ◽  
Chinese Herbal ◽  
Alpinia Oxyphylla ◽  
Medicinal Value

Abstract Background: Yizhiren is the fruit of Alpinia oxyphylla(Zingiberaceae) ,a well-known Chinese herbal medicine from China. The complete chloroplast genome of Alpinia oxyphylla was studied in this paper, which laid the foundation of the further study of genetic information and data of Alpinia oxyphylla.Methods: The complete chloroplast sequences of 19 the family Zingiberaceae species were aligned using MEGAX software.The phylogenetic tree was constructed by the Maximum-Likelihood method and edited by the Evolview online.Results: The chloroplast gene group is a typical tetragonal structure, which is formed by 161,351 base pairs. Each genome has a large single-copy region (LSC) of 87,248 bp, a small single-copy region (SSC) of 16,175 bp and a pair of inverted-repeat regions (IRs) of 28,964 bp in each. The complete nucleotide composition of chloroplast genome is: 31.5% A, 32.4% T, 18.2% C, 17.9% G, and the total GC content is 36.2%. Among them 28 exons and 15 introns. A total of 137 genes were annotated, which included 92 protein coding genes (PCGs), 37 metastatic RNA (tRNAs) and 8 ribosomal RNA (rRNAs).Conclusions: The phylogenetic ML tree shown the conclusion that Alpinia oxyphylla is closely related to Alpinia chinensis on genetic position relationship. This result is of great value to the study of biological inheritance, species identification and medicinal value. Meanwhile, it provides references for the study of biological inheritance, species identification and medicinal value.

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