Diagnosis of Hypersensitivity Induced by Antituberculosis Drugs

Objective. To explore the clinical value of the specific plasma cell detection and specific T lymphocyte detection test in diagnosing hypersensitivity caused by antituberculosis drugs. Methods. A total of 266 patients with pulmonary tuberculosis who developed hypersensitivity during the treatment of primary pulmonary tuberculosis in our hospital and 266 patients without hypersensitivity during the treatment of pulmonary tuberculosis in our hospital were selected as the control group. The admission time is from January 2013 to June 2020. The specific plasma cell test and specific T lymphocyte test were used as the criteria to determine which drugs induced hypersensitivity, and the diagnostic value of these two methods in the diagnosis of hypersensitivity induced by four first-line antituberculosis drugs (isoniazid (INH), ethambutol (EMB), rifampicin (RFP), and pyrazinamide (PZA)) was analyzed. Results. The sensitivity of the specific plasma cell test in the diagnosis of hypersensitivity induced by INH, EMB, RFP, and PZA was 63.42%, 51.20%, 47.81%, and 56.37%, respectively, and the specificity was 95.33%, 99.87%, 96.52%, and 99.99%, respectively. The sensitivity of the specific T lymphocyte test in the diagnosis of hypersensitivity induced by INH, EMB, RFP, and PZA was 66.47%, 52.88%, 49.91%, and 58.54%, respectively, and the specificity was 97.28%, 99.99%, 98.38%, and 100.00%, respectively. Conclusion. The specific plasma cell test and specific T lymphocyte test have high specificity in the diagnosis of hypersensitivity caused by antituberculosis drugs, and the specific T lymphocyte test is better than the specific plasma cell test. It is of great significance to guide the clinical application of antituberculosis drugs.

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Significance of peripheral blood indexes in differential diagnoses of SARS-CoV-2 and New Bunia virus

Scientific Reports ◽

10.1038/s41598-021-93519-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Wentao He ◽

Xiaoyi Liu

Keyword(s):

Differential Diagnosis ◽

T Lymphocyte ◽

Roc Curves ◽

Diagnostic Value ◽

Blood Parameters ◽

Control Group ◽

Clinical Value ◽

T Lymphocyte Subsets ◽

Reactive Protein ◽

Tnf Α

AbstractWe aimed to provide a laboratory basis for differential diagnosis of COVID-19 and severe fever with thrombocytopenia syndrome (SFTS). Clinical data were collected from 32 COVID-19 patients (2019-nCoV group), 31 SFTS patients (SFTS group) and 30 healthy controls (control group). For each group of hospitalized patients, a retrospective analysis was performed on specific indices, including cytokines, T-lymphocyte subsets, routine blood parameters, C-reactive protein (CRP) and procalcitonin (PCT), and receiver operating characteristic (ROC) curves for the indices revealed the differences among groups. Compared with the 2019-nCoV group, the SFTS group had a significantly and greatly decreased counts of WBC, absolute lymphocyte, PLT and absolute CD4+ T lymphocyte (P < 0.05); the IL-6, TNF-α, D-D and PCT levels of the SFTS group were higher than those of the 2019-nCoV group (P < 0.05). Compared with those of the SFTS group, the CRP and FIB levels of the 2019-nCoV group were greatly increased (P < 0.05). The ROC curves showed that area under the curves (AUCs) for FIB, PLT and TNF-α were greater than 0.85, demonstrating high diagnostic value. At the initial stage of SARS-CoV-2 or SFTS virus infection, PLT, FIB and TNF-α have definitive clinical value for the early and differential diagnosis of these two infections.

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Diagnostic value of serum IgG by Eliza to detecting Mycobacterium tuberculosis, Original Article

Journal of Research in Clinical Medicine ◽

10.34172/jrcm.2021.029 ◽

2021 ◽

Vol 9 (1) ◽

pp. 29-29

Author(s):

Mehdi Haghdoost ◽

Parisa Alizadeh Nazmi ◽

Hamid Owaysee Osquee

Keyword(s):

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Immunoglobulin G ◽

Diagnostic Value ◽

World Health ◽

Control Group ◽

Reference Laboratory ◽

Predictive Values ◽

Serum Igg ◽

Significant Difference

Introduction: In developing countries, which is an endemic region in terms of tuberculosis, there is an urgent need for fast, accurate, and inexpensive serological testing. The aim of this study was to determine the diagnostic value of patient serum IgG antibodies by ELISA in the diagnosis of Mycobacterium tuberculosis. Method: This case-control study was performed on patients with pulmonary tuberculosis in 2017-2020. After selecting the case (n = 30) and control (n = 30) subjects according to inclusion criteria, their blood samples were obtained and analyzed in the reference laboratory by standard kits for immunoglobulin G against 16, 36, and 40 kDa antigens of mycobacterium tuberculosis. Results: The mean age of the subjects was 47.07 (15.57%). The majority of participants were 46 (51.1%) women. There was no significant difference between the two groups regarding sex and age. serological examination of patients with pulmonary tuberculosis showed 25 positive results and only 4 of the control group had a positive result. Sensitivity, specificity, positive and negative predictive values of serology test were 83.3%, 86.67%, 86.20% and 87.88% respectively. Conclusion: Despite the acceptable sensitivity of the serologic immunoglobulin G test, according to the statement of World health organization (WHO), it did not possess an acceptable specificity. It is recommended that a a wider range of different antigens to be studied also it is essential to evaluate the diagnostic value of the other immunoglobulins inpatient in different stages of the disease.

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MicroRNA-183-5p acts as a potential diagnostic biomarker for atherosclerosis and regulates the growth of vascular smooth muscle cell

10.21203/rs.2.14613/v2 ◽

2019 ◽

Author(s):

Bin Sun ◽

Zhengkun Shan ◽

Guoyu Sun ◽

Xiaolong Wang

Keyword(s):

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Roc Analysis ◽

High Specificity ◽

Diagnostic Value ◽

Clinical Value ◽

Specificity And Sensitivity ◽

And Migration ◽

Vsmcs Proliferation ◽

Proliferation And Migration

Abstract Background Atherosclerosis (AS) is a multifactorial chronic disease, and vascular smooth muscle cells (VSMCs) plays an important role in the pathology of AS. MicroRNAs regulate multiple cellular biological processes. This study aimed to investigate the clinical value of miR-183-5p in AS patients, and further explored the effects of miR-183-5p on the proliferation and migration of VSMCs. Methods qRT-PCR was used to test the level of miR-183-5p. The diagnostic value of miR-183-5p for AS patients was assessed by a receiver operating characteristic (ROC) analysis. Cell proliferation and migration were determined via CCK-8 and Transwell assay. Results MiR-183-5p was highly expressed in AS patients compared with the healthy group. Serum miR-183-5p expression was positively associated with CIMT and CRP in AS patients. The ROC analysis suggested that miR-183-5p had quality to be used as a biomarker with high specificity and sensitivity for AS detection. Overexpression of miR-183-5p promoted the proliferation and migration of VSMCs. Downregulation of miR-183-5p attenuated ox-LDL stimulated VSMCs proliferation and migration. Conclusion MiR-183-5p is highly expressed in AS patients, and downregulation of miR-183-5p attenuated ox-LDL stimulated VSMCs proliferation and migration. MiR-183-5p may be a key molecular for the diagnosis and treatment of AS in the future.

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S235 – Diagnostic Test Validation: CHAMP in Endolymphatic Hydrops

Otolaryngology ◽

10.1016/j.otohns.2008.05.410 ◽

2008 ◽

Vol 139 (2_suppl) ◽

pp. P154-P154

Author(s):

Eveling Y Rojas ◽

Leonardo Ordoñez ◽

Victor Hernandez ◽

Jose E Guzman ◽

Juan Izquierdo ◽

...

Keyword(s):

Diagnostic Test ◽

Endolymphatic Hydrops ◽

False Negative ◽

High Specificity ◽

Diagnostic Value ◽

Clinical Value ◽

Negative Results ◽

False Negative Results ◽

The Relationship

Objectives The Cochlear Hydrops Analysis Masking Procedure (CHAMP) has been reported with a high sensibility and specificity for the diagnosis of endolymphatic hydrops (ELH). Nevertheless, a complete validation of CHAMP with precise methods has not been done. Our objectives: To determine whether CHAMP has a diagnostic value in patients with definite, probable/possible diagnosis of Meniere's disease or endolymphatic hydrops, based on the criteria of the AAO-HNS. Methods Prospective validation study for a diagnostic test: phase II. Latency of wave V was assessed using CHAMP. We classified subjects in 5 groups: 1-Definite ELH, 2-Probable ELH, 3-Possible ELH, 4-subjects with other otovestibular symptoms, 5-normal hearing subjects. We included cases (groups 1–4) with an episode of vertigo with duration equal or longer than 20 minutes, with or without other otovestibular symptoms. Results We included 67 cases (132 ears). 38 cases (76 ears) completed the follow up and their results are presented. In group 1, the sensibility of CHAMP was 43% and its specificity was 100%. In groups 2–3, the sensitivity was 20% and the specificity 100%, with 0% false positive and 80% false negative results. Other indicators of validity for this diagnostic test are presented. Conclusions CHAMP has a high specificity and low sensibility for the diagnosis of ELH when the delay in wave V latency is used. Future studies may assess the relationship between wave amplitudes to improve the clinical value of CHAMP.

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Diagnostic value of three simple and rapid dry eye tests: Lid parallel conjunctival folds, tear meniscus height and tear ferning

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh190829024d ◽

2021 ◽

pp. 24-24

Author(s):

Bojana Dacic-Krnjaja ◽

Milan Hadzi-Milic ◽

Jelena Potic ◽

Danijela Raonic ◽

Milenko Stojkovic

Keyword(s):

Dry Eye ◽

High Specificity ◽

Diagnostic Value ◽

Control Group ◽

Ocular Surface Disease Index ◽

Mean Values ◽

Meniscus Height ◽

Tear Meniscus ◽

Tear Meniscus Height ◽

Low Sensitivity

Introduction/Objective. The objective of this paper was to assess the diagnostic value of three simple dry eye (DE) tests: lid parallel conjunctival folds (LIPCOF), tear meniscus height (TMH), and tear ferning (TF). Methods. Diagnostic DE tests LIPCOF, TMH and TF tests were performed in 100 patients. Eighty of them were referred to us by rheumatologists and general practitioners either during evaluation for Sj?gren?s syndrome, or because of DE symptoms. Control group was made of 20 patients, with no DE relating symptoms. Ocular Surface Disease Index (OSDI) questionnaire was used for DE symptoms? evaluation. Results of LIPCOF, TMH and TF tests were compared with results of Copenhagen criteria (CC) DE tests i.e., tear fluorescein break up time, Schirmer I and Rose Bengal tests. Ability of tests to recognize DE in various grades according to Dry Eye Work Shop (DEWS) report score system was assessed. Results. Compared to CC, sensitivity of LIPCOF and TMH was high: 92.8% and 83.5%, while specificity was low: 34.4% and 49.2%, respectively. TF had low sensitivity of 59.1% but high specificity of 82.7%. Mean values of both LIPCOF and TMH differed significantly (F = 7.222, p < 0.001 and F = 11.802, p < 0.001) between control group and all DEWS DE grades, but not among different grades of DE. Conclusions. Diagnostic tests TMH and LIPCOF showed high sensitivity which makes them excellent screening DE tests. Low sensitivity of TF suggests that it is not truly a good screening test on its own, but its high specificity is of definite value.

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A predictive model to calculate the probability of placental abruption in patients with initially low obstetric risk

GYNECOLOGY ◽

10.26442/20795696.2020.4.200150 ◽

2020 ◽

Vol 22 (4) ◽

pp. 6-10

Author(s):

Nataly I. Frolova ◽

Tatiana E. Belokrinitskaya

Keyword(s):

Predictive Model ◽

Predictive Models ◽

Placental Abruption ◽

High Specificity ◽

Diagnostic Value ◽

Individual Risk ◽

Control Group ◽

Quality Model ◽

History Of ◽

Pai 1

Aim. To develop a predictive model to calculate the probability of placental abruption development in patients with initially low obstetric risk by using a multidimensional mathematical analysis of anamnestic, clinical, and genetic data. Materials and methods. This logistic model is based on 212 observations. This study included two groups of healthy women aged 1835 years without a history of spontaneous abortion, preterm labor, stillbirth, preeclampsia, or other complications. The first group was comprised of 112 women with a history of a placental abruption (patient group), and the second group consisted of 100 women with non-complicated pregnancy (control group). Gene polymorphisms were detected using the polymerase chain reaction-real time technique. The data was analyzed using binary and multifactorial statistical mathematics. Our analysis of the predictive models was performed by using logistic regression. To determine the diagnostic value of the predictive models used, the ROC-curve is followed by determining the area under it (AUC). Results. The 24 anamnestic, clinical, and genotypic characteristics were examined as placental abruption predictors using multifactorial analysis. We were able to determine what the nine most informative predictors of placental abruption were according to Wald Chi-Square criteria (2, р): fetal growth restriction (13.143; р0.001), placental insufficiency (9.629; р=0.002), male sex of the fetus (6.965; р=0.008), preeclampsia (6.779; р=0.009), combination of genotypes PAI-1-5G4G/FV-1691GA (6.613; р=0.010) or PAI-1-5G4G/MTHFR-677CT (6.158; р=0.013), pathology of amnion (4.497; р=0.034), infectious diseases in pregnancy (4.277; р=0.039), tobacco smoking (3.963; р=0.047). ROC analysis identified a high specificity (82.64%) and sensitivity (77.42%) of the model, and the integral index of the effectiveness of predictive markers (AUC=0.836), according to the expert scale of values which is indicative of a very high quality model. Conclusion. It is recommended to use this elaborated predictive model in clinical practice for the purpose of individual risk assessment of placental abruption in healthy patients with initially low obstetric risk.

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Clinical Diagnostic Value for Colorectal Cancer Based on Serum CEA, CA24-2 and CA19-9

10.21203/rs.3.rs-19403/v2 ◽

2020 ◽

Author(s):

Hui Rao ◽

Heming Wu ◽

Qingyan Huang ◽

Zhikang Yu ◽

Qunji Zhang ◽

...

Keyword(s):

Colorectal Cancer ◽

Tumor Markers ◽

Diagnostic Value ◽

Carbohydrate Antigen ◽

Control Group ◽

Sensitivity Index ◽

Depth Of Invasion ◽

Clinical Value ◽

Braf Mutations ◽

Serum Cea

Abstract Background To explore the clinical value of a combined detection of serum concentration of carcinoembryonic antigen (CEA), carbohydrate antigen 24-2 (CA24-2), and carbohydrate antigen 19-9 (CA19-9) for colorectal cancer (CRC). Methods The levels of serum tumor markers (CEA, CA24-2 and CA19-9) and clinical characteristics in patients with colorectal cancer were evaluated. In addition, KRAS/NRAS/PIK3CA/BRAF mutations were detected in some patients with colorectal cancer. Results A total of 2,281 patients were recruited in the study, included 1,578 colorectal cancer patients and 703 controls. The levels of CEA, CA24-2 and CA19-9 in colorectal cancer group was significantly higher than control group. The sensitivities of three individual markers were lower than 30%, which individual sensitivity of the tumor markers sorted in descending order was CEA>CA19-9>CA24-2. The specificities of three individual markers were more than 92%, and the specificity sorted in descending order was CA24-2>CA19-9>CEA. The combination of CEA+CA19-9+CA24-2 ranked the highest in sensitivity index and specificity index for colorectal cancer diagnosis. The prediction equation excluding the risk of colorectal cancer was. Probability (normal) = Exp (-5.47 - 0.28CEA - 0.11 CA242 + 0.001 CA199)/ (1+ Exp (-5.47 - 0.28CEA - 0.11 CA242 + 0.001 CA199)). There were no significant differences in age, gender, histology type, differentiation, depth of invasion and TNM stage between mutations in KRAS/NRAS, BRAF and PIK3CA genes or not, respectively. Conclusions Serum CEA, CA24-2, and CA19-9 are valuable noninvasive indicators for prediction the risk of colorectal cancer. We need to look for other, more sensitive tumor markers.

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MicroRNA-183-5p acts as a potential diagnostic biomarker for atherosclerosis and regulates the growth of vascular smooth muscle cell

10.21203/rs.2.14613/v3 ◽

2020 ◽

Author(s):

Bin Sun ◽

Zhengkun Shan ◽

Guoyu Sun ◽

Xiaolong Wang

Keyword(s):

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Roc Analysis ◽

High Specificity ◽

Diagnostic Value ◽

Clinical Value ◽

Specificity And Sensitivity ◽

And Migration ◽

Vsmcs Proliferation ◽

Proliferation And Migration

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Value of radiographic measurements in depiction of glenohumeral and acromial anatomical changes in patients with subacromial impingement syndrome and rotator cuff tears

Medical Visualization ◽

10.24835/1607-0763-2018-3-98-108 ◽

2018 ◽

pp. 98-108

Author(s):

V. E. Gazhonova ◽

Е. M. Bachurina ◽

M. V. Emelianenko ◽

I. E. Popova ◽

T. A. Soina ◽

...

Keyword(s):

Rotator Cuff ◽

Mr Arthrography ◽

Diagnostic Value ◽

Rotator Cuff Tears ◽

Subacromial Impingement Syndrome ◽

Control Group ◽

Clinical Value ◽

Mean Values ◽

Radiographic Measurements ◽

And Control

The purpose.Optimization of radiographic study of the shoulder in patients with SIS and estimation of the clinical value of radiographic measurements in SIS diagnosis and rotator cuff tears (RCT).Materials and methods.128 patients with a painful shoulder (67 women, 61 men), mean age 57.3 ± 12.8 y.o. and 35 patients without shoulder pain mean age 56 ± 10.4 y.o. underwent a complex study including radiography, MRI, MDCTor MR-arthrography, arthroscopy. Special angles and distances were estimated on a Radiographic Work Station by 2 radiologists (acromion tilt (AT), acromion slope, inferior acromion protrusion, acromio-humeral distance, acromio-humeral index, critical shoulder angle (CSA), lateral acromion angle, transverse acromion angle). Mean values of these parameters were compared between patients with SIS and control group, and between patients with RCT and without . Test-retest reproducibility and inter rater agreement were calculated with Kohen’s kappa. Diagnostic value of the most informative parameters was compared with estimation of AUC under the ROC curve.Results.CSA and AT demonstrated independence with age and excellent test-retest reproducibility. In SIS patients mean values of CSA were statistically higher (35.78 ± 4.64°), AT values – lower (25.90 ± 4.27°), then in controls. In all patients with CSA more then 35, the frequency of RCT was 74%. In patients with RCT were detected higher values of CSA (36.70 ± 2.61°) and lower values of AT (23.67 ± 3.07°), then in patients without tears. Lateral acromion angle (acromion type) demonstrated correlation with patient’s age and moderate reproducibility in test-retest studies.Conclusion.AT (measured on the outlet X-ray view) and CSA (measured in direct views) could be recommended for clinical use for evaluation of patients with SIS and prognosing RCT.

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Clinical Diagnostic Value for Colorectal cancer Based on Serum CEA, CA24-2 and CA19-9

10.21203/rs.3.rs-19403/v1 ◽

2020 ◽

Author(s):

Hui Rao ◽

Heming Wu ◽

Qingyan Huang ◽

Zhikang Yu ◽

Qunji Zhang ◽

...

Keyword(s):

Colorectal Cancer ◽

Tumor Markers ◽

Diagnostic Value ◽

Prediction Equation ◽

Carbohydrate Antigen ◽

Control Group ◽

Depth Of Invasion ◽

Clinical Value ◽

Braf Mutations ◽

Serum Cea

Abstract Background To explore the clinical value of a combined detection of serum concentration of carcinoembryonic antigen (CEA), carbohydrate antigen 24-2 (CA24-2), and carbohydrate antigen 19-9 (CA19-9) for colorectal cancer (CRC). Methods The levels of serum tumor markers (CEA, CA24-2 and CA19-9) and clinicopathologic features in patients with colorectal cancer in Meizhou People's Hospital were evaluated. In addition, KRAS/NRAS, PIK3CA and BRAF mutations were detected in some patients with colorectal cancer. Results A total of 2,281 patients (1,420 males and 861 females) were recruited in the study, included 1,578 colorectal cancer patients and 703 controls. The levels of CEA, CA24-2 and CA19-9 in colorectal cancer group was significantly higher than control group. The sensitivities of three individual markers were lower than 30%, which individual sensitivity of the tumor markers sorted in descending order were CEA> CA19-9> CA24-2. The specificities of three individual markers were more than 92%, and the specificity sorted in descending order were CA24-2> CA19-9> CEA. The prediction equation excluding the risk of colorectal cancer was. Probability (normal) = Exp (-5.47 - 0.28CEA - 0.11 CA242 + 0.001 CA199)/ (1+ Exp (-5.47 - 0.28CEA - 0.11 CA242 + 0.001 CA199)). There were no significant differences in age, gender, histology type, differentiation, depth of invasion and TNM stage between mutations in KRAS/NRAS , BRAF and PIK3CA genes or not, respectively. Conclusions Serum CEA, ca24-2, and ca19-9 are valuable noninvasive indicators for the detection and screening of patients with early colon cancer. We need to look for other, more sensitive tumor markers.

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