scholarly journals Investigation of Dried Blood Spot Cards for Fatty Acid Analysis Using Porcine Blood

2021 ◽  
Vol 2021 ◽  
pp. 1-4
Author(s):  
Jordan Wood ◽  
Larry J. Minter ◽  
Michael K. Stoskopf ◽  
Doug Bibus ◽  
Dempsy Ange ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Whole Blood ◽  
Fatty Acid Analysis ◽  
Dried Blood Spot ◽  
Nervonic Acid ◽  
Blood Collection ◽  
Omega 3 ◽  
Blood Samples ◽  
Blood Spot

Fatty acids, especially omega-3 and omega-6 fatty acids, are important for reproductive and cardiovascular health in animals. While monitoring fatty acids is traditionally conducted using frozen blood fractions such as serum and plasma, advancements in analytical technology have developed a method of collecting microsamples of dried whole blood on Ahlstrom 226 grade filter paper that can provide information on long-term fatty acid status of animals. Blood samples were collected from five male pigs in both the traditional frozen method and on dried blood spot cards (DBS). The DBS samples were collected with untreated syringes and tubes, and approximately 320 μL of blood was placed on each card with approximately 80 μL per spot (4 spots). Statistical analysis was performed to compare the two sample groups to each other using the Mann–Whitney U-test and determine if DBS samples were similar to traditional whole blood samples. Of the 30 fatty acids and fatty acid groups with measurable concentrations, only four individual fatty acids, behenic acid, omega-3 docosapentaenoic acid, nervonic acid, and adrenic acid, had statistical differences. Most of these differences were minor and could be due to analytical errors or contamination. Comparisons between sample types found similar concentrations of key omega fatty acids and PUFAs and support the use of DBS collection as a less invasive method of blood collection and fatty acid analysis.

scholarly journals Comparison of African savanna elephant (Loxodonta africana) fatty acid profiles in whole blood, whole blood dried on blood spot cards, serum, and plasma

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12650
Author(s):  
Jordan Wood ◽  
Larry J. Minter ◽  
Doug Bibus ◽  
Michael K. Stoskopf ◽  
Vivek Fellner ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Whole Blood ◽  
Health Assessment ◽  
Field Research ◽  
Blood Collection ◽  
Fatty Acid Profiles ◽  
Omega 3 ◽  
African Elephants ◽  
Free Ranging

Background African elephants in managed care have presented differences in the balance between omega-3 and omega-6 fatty acids, a situation primarily thought to be due to dietary differences between the managed animals and their free-ranging counterparts. Because of this, circulating fatty acid status is included in routine monitoring of elephant health. A method of blood collection that requires only a few drops of whole blood, dried on filter paper (DBS) and can be used for analyzing full fatty acid profiles offers advantages in clinical application. Methods This study compared the use of whole blood, and whole blood DBS, serum or plasma for use in evaluating circulating fatty acid composition in African savannah elephants. Samples from six African elephants (two males and four females) were collected during the same week at the NC Zoo, Asheboro, NC. Results Results found only 2 of 36 individual fatty acids and none of the 10 fatty acid groupings were different when comparing the four blood fraction sample types to each other with Mann-Whitney U-Test pairwise comparisons. Myristic acid (14:0) was lower in the DBS samples than in whole blood, serum, and plasma and pentadecaenoic acid (15:1) was slightly more concentrated in DBS and whole blood. Discussion Results indicate that fatty acid profile of serum, plasma, whole blood, and DBS are comparable in African elephants. The DBS method offers advantages in acquisition and handling and may be preferable to other methods in both routine health assessment of captive animals and field research on free ranging animals.

Development and validation of a dried blood spot–HPLC assay for the determination of metronidazole in neonatal whole blood samples

2010 ◽  
Vol 397 (2) ◽  
pp. 687-693 ◽  
Author(s):  
Maysa Faisal Suyagh ◽  
Godwill Iheagwaram ◽  
Prashant Laxman Kole ◽  
Jeff Millership ◽  
Paul Collier ◽  
...  
Keyword(s):  
Whole Blood ◽  
Dried Blood Spot ◽  
Hplc Assay ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Development And Validation ◽  
Blood Spot

Characterization of fatty acids and nutrient composition of SAU Perilla-1 [(Perilla frutescens (L.) Britton] seeds grown with agro climatic conditions in Bangladesh

2021 ◽  
Vol 27 (02) ◽  
pp. 2307-2314
Author(s):  
M. A. K. Mojumdar ◽  
H. M. M. T. Hossain ◽  
A. F. M. J. Uddin ◽  
Meherunnessa
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Essential Fatty Acids ◽  
Fatty Acid Analysis ◽  
Climatic Conditions ◽  
Omega 3 ◽  
Plant Seed ◽  
Omega 6 ◽  
Crop Variety

SAU Perilla-1 (Golden perilla BD) is a newly edible oil seed crop variety introduced by Sher-e-Bangla Agricultural University in Bangladesh. Its seed oil is a rich source of unsaturated fatty acid (91%), of which more than 50% is α-linolenic acid (type of omega-3 fatty acid). The estimated ratio of saturated, monounsaturated and polyunsaturated fatty acids was found 1: 2.26: 8.95. Moreover, the α-linolenic acid was detected (50.52%) as the most dominating polyunsaturated fatty acid, which was 4-four times higher than monounsaturated fatty acids in the oil of the crop variety. Fatty acid analysis of oil revealed a ratio (1:2.22) of Omega 6 to Omega 3 fatty acids, which lies within a healthy range as documented by the global scientific community. Compared to other plant seed oils, SAU Perilla-1 oil consists of Linoleic acid (Omega 6 fatty acid, 22.71%) – a component associated with obesity, which is far below the regular oils from soybean, sunflower and corn. Therefore, our findings indicated that SAU Perilla-1 seed is one of the best edible sources of plant oils rich in essential fatty acids conducive to human health.

The influence of a basic military training diet on whole blood fatty acid profile and the Omega-3 Index of Australian Army recruits.

2021 ◽  
Author(s):  
Gregory E. Peoples ◽  
Penelope Larsen ◽  
Heather M Bowes ◽  
Jarrin Coombes ◽  
Jace R Drain ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Eicosapentaenoic Acid ◽  
Fatty Acid Profile ◽  
Whole Blood ◽  
Military Training ◽  
Omega 3 ◽  
Blood Samples ◽  
Finger Prick ◽  
Omega 6 ◽  
Army Recruits

This study described the whole blood fatty acid profile and Omega-3 Index (O3I) of Australian Army recruits at the commencement and completion of basic military training (BMT). Eighty (80) males (17-34 y, 77.4±13.0 kg, 43.5±4.3 mL/kg/min) and 37 females (17-45 y, 64.3±8.8 kg, 39.3±2.7 mL/kg/min) volunteered to participate (N=117). Whole blood samples of each recruit were collected using a finger prick in weeks 1 and 11 (n=82) and analysed via gas chromatography for the relative proportions of each fatty acid (mean [95% CI]). The macronutrient characteristics of the diet offerings was also determined. At commencement there was a low omega-3 status (sum of omega-3; 4.95% [4.82-5.07]) and O3I (5.03% [4.90-5.16]) and no recruit recorded an O3I >8% (desirable). The omega-6/omega-3 (7.04 [6.85-7.23]) and arachidonic acid / eicosapentaenoic acid (AA/EPA) (18.70 [17.86-19.53]) ratios for the cohort were also undesirable. The BMT mess menu provided a maximum of 190 mg/day of eicosapentaenoic acid (EPA) and 260 mg/day of docosahexaenoic acid (DHA). The O3I of the recruits was lower by week 11 (4.62% [4.51-4.78], p<0.05), the omega-6/omega-3 increased (7.27 [7.07-7.47] p<0.05) and the AA/EPA remained elevated (17.85 [16.89-18.81]). In conclusion, Australian Army recruits’ omega-3 status remained undesirable during BMT and deserves nutritional attention. Novelty Bullets • Australian Army recruits’ Omega-3 Index, at the commencement of BMT, was reflective of the Western-style diet. • The BMT diet offered minimum opportunity for daily EPA and DHA consumption. • Every recruit experienced a further reduction of their Omega-3 Index during BMT.

On-line desorption of dried blood spot: A novel approach for the direct LC/MS analysis of μ-whole blood samples

2009 ◽  
Vol 49 (4) ◽  
pp. 1034-1039 ◽  
Author(s):  
Julien Déglon ◽  
Aurélien Thomas ◽  
Antonio Cataldo ◽  
Patrice Mangin ◽  
Christian Staub
Keyword(s):  
Whole Blood ◽  
Dried Blood Spot ◽  
Blood Samples ◽  
Ms Analysis ◽  
Novel Approach ◽  
On Line ◽  
Whole Blood Samples ◽  
Blood Spot

Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection

2012 ◽  
Vol 32 (8) ◽  
pp. 547-556 ◽  
Author(s):  
Adam H. Metherel ◽  
Lindy M. Buzikievich ◽  
Payman Charkhzarin ◽  
Ashley C. Patterson ◽  
Alexandrea C. Peel ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Whole Blood ◽  
Blood Collection ◽  
Omega 3 ◽  
Fatty Acid Profiling ◽  
Overnight Fasting

Nervonic Acid (24:1n-9) is a Dominant Unsaturated Fatty Acid in the Intestinal Brush Border of Atlantic Cod

2012 ◽  
Vol 5 ◽  
pp. LPI.S10291 ◽  
Author(s):  
Gudjón Andri Gylfason ◽  
Erna Knútsdóttir ◽  
Bjarni Ásgeirsson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Brush Border ◽  
Atlantic Cod ◽  
Basolateral Membrane ◽  
Monounsaturated Fatty Acids ◽  
Cholesterol Content ◽  
Fatty Acid Analysis ◽  
Nervonic Acid ◽  
Nutrient Selection

Atlantic cod is a coldwater teleost of commercial importance. The intestinal epithelium is a large organ in vertebrates serving an important role in nutrient selection and uptake as well as an immunological barrier. Here, we perform lipid and fatty acid analysis of the plasma membrane from the cod intestinal enterocytes after separation of the brush border membrane and the basolateral membrane fractions. Our results show that both membrane fractions contain an unusually high amount of cholesterol and glycolipids but low levels of glycerophospholipids compared with other reported studies on fish. Sphingomyelin was the dominant lipid in the brush border fraction and was also prominent in the basolateral fraction where phosphatidylcholine was the dominant glycerophospholipid. Furthermore, our results show a distinct difference in fatty acids content, where monounsaturated fatty acids (MUFA) were more abundant than polyunsaturated fatty acid (PUFA). Nervonic acid (24:1n-9) was a prominent fatty acid in the BBM at ~50% of the total MUFA. We hypothesize that the high cholesterol content and the presence of this rare fatty acid may serve to maintain membrane fluidity in the cold environment.

scholarly journals Fingertip Whole Blood as an Indicator of Omega-3 Long-Chain Polyunsaturated Fatty Acid Changes during Dose-Response Supplementation in Women: Comparison with Plasma and Erythrocyte Fatty Acids

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1419
Author(s):  
Barbara J. Meyer ◽  
Cassandra Sparkes ◽  
Andrew J. Sinclair ◽  
Robert A. Gibson ◽  
Paul L. Else
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Whole Blood ◽  
Premenopausal Women ◽  
Chain Polyunsaturated Fatty Acid ◽  
Omega 3 ◽  
Epa And Dha ◽  
Dose Dependent ◽  
Long Chain

The sensitivity of fingertip whole blood to reflect habitual dietary and dose-dependent supplemental omega-3 long-chain polyunsaturated fatty acid (n-3 LCPUFA) intake in premenopausal women was compared to that of venous erythrocytes and plasma fatty acids. Samples were obtained from women in a randomised, double-blind, placebo-controlled trial in which premenopausal women (n = 53) were supplemented with DHA-rich tuna oil capsules and/or placebo (Sunola oil) capsules (6 capsules per day) for 8 weeks to achieve doses of either 0, 0.35, 0.7 or 1.05 g/day n-3 LCPUFA. All blood biomarkers were very similar in their ability to reflect dietary n-3 LCPUFA intake (r = 0.38–0.46 for EPA and DHA intake), and in their dose-dependent increases in n-3 LCPUFA levels after supplementation (R2 = 0.41–0.51 for dose effect on biomarker EPA and DHA levels (mol %)). Fingertip whole blood is an effective alternative to erythrocytes and plasma as a biomarker n-3 LCPUFA intake in premenopausal women.

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

EFFECT OF GINGER, LEMURU FISH OIL SAPONIFICATION AND OLIVE OIL ON COMPOSITION FATTY ACID OF BEEF

2014 ◽  
Vol 4 (1) ◽  
pp. 31-39
Author(s):  
Siwitri Kadarsih
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Olive Oil ◽  
Rice Bran ◽  
Dry Matter ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Omega 3 ◽  
Omega 6

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords : 

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