scholarly journals The Effect of the Addition of Chokeberry Fiber on the Quality of Sheep’s Milk Fermented by Lactobacillus rhamnosus and Lactobacillus acidophilus

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
K. Szajnar ◽  
M. Pawlos ◽  
A. Znamirowska
Keyword(s):  
Lactobacillus Acidophilus ◽  
Ph Value ◽  
Control Sample ◽  
Lactobacillus Rhamnosus ◽  
Fermented Milk ◽  
Global Market ◽  
Microbiological Analysis ◽  
Bacterial Cells ◽  
Organoleptic Evaluation ◽  
Sheep Milk

The global market for sheep’s milk and its products is increasing due to higher demand for cheese and traditional dairy products, and as a novelty, sheep’s milk is an ingredient in infant formulas and nutraceuticals. The aim of this study was to determine the properties of fermented sheep’s milk, which combines probiotic benefits with increased dietary fiber content. The influence of the applied dose of chokeberry fiber on the growth of living cells of Lactobacillus acidophilus and Lactobacillus rhamnosus in fermented sheep’s milk was also evaluated. Sheep milk with the addition of 0% (control sample), 1.5%, and 3.0% chokeberry fiber was fermented by two different probiotic monocultures L. acidophilus and L. rhamnosus. In the fermented milk, pH value, syneresis (%), color, and texture were determined. Furthermore, the microbiological analysis and an organoleptic evaluation were performed. With the increasing dose of chokeberry fiber, the pH value decreased already before the fermentation process. After fermentation, the milk’s pH values with fiber were still lower than those in the control sample. Presumably, more acidic metabolites were produced by L. acidophilus, causing a more intense reduction of the pH value than L. rhamnosus both in control milk and in milk with the addition of fiber. The addition of chokeberry fiber affected the stimulation of the growth of both types of bacteria. In the milk sample without fiber addition, a more significant number of viable cells were counted for L. acidophilus by 0.5 log CFU g-1 more than the milk fermented with L. rhamnosus. Furthermore, in milk fermented by L. acidophilus with 1.5% chokeberry fiber (LA1), the number of viable bacterial cells was higher than that in milk fermented by L. rhamnosus with the same addition of fiber (LR1). However, in sample LA3, the number of viable bacterial cells was lower than that in sample LR3. Tested fermented sheep milk met the Recommendation of the International Dairy Federation’s requirements regarding the number of live bacterial cells for dairy probiotics. The addition of chokeberry fiber increased syneresis in each fermented milk group, regardless of the bacteria used for fermentation. Moreover, the use of fiber caused a significant reduction in brightness L ∗ , an increase of red color, and a decrease of yellow color. Milk fermented with L. acidophilus was characterized by a harder gel, compared to their analogues fermented with L. rhamnosus. The addition of fiber intensified the sour taste and the taste of the additive in both types of fermented sheep milk.

scholarly journals The Use of Olkuska Sheep Milk for the Production of Symbiotic Dairy Ice Cream

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 70
Author(s):  
Magdalena Kowalczyk ◽  
Agata Znamirowska ◽  
Małgorzata Pawlos ◽  
Magdalena Buniowska
Keyword(s):  
Lactobacillus Acidophilus ◽  
Ph Value ◽  
Ice Cream ◽  
Partial Replacement ◽  
Freezing Process ◽  
Bacterial Cells ◽  
Organoleptic Properties ◽  
Sheep Milk ◽  
Lactic Acid Content ◽  
Drop Time

The aim of this study was to determine the possibility of using Olkuska sheep milk for the production of ice cream with probiotics and prebiotics. The study examined the effect of the storage and type of bacteria used for the fermentation of ice cream mixes and partial replacement of inulin with apple fiber on the physicochemical properties, viability of probiotic cultures and organoleptic properties of sheep’s milk ice cream stored at −22 °C for 21 days. The addition of apple fiber reduced the pH value of ice cream mixes before fermentation. In ice cream mixes and ice cream with apple fiber, the lactic acid content was higher by 0.1–0.2 g L−1 than in their equivalents with inulin only. These differences persisted during the storage of the ice cream. After fermentation of the ice mixes, the bacterial cell count ranged from 10.62 log cfu g−1 to 12.25 log cfu g−1. The freezing process reduced the population of probiotic bacteria cells in ice cream with inulin from 0.8 log cfu g−1 in ice cream with Lactobacillus acidophilus, 1.0 log cfu g−1 in ice cream with Lacticaseibacillus paracasei and 1.1 log cfu g−1 in ice cream with Lacticaseibacillus casei. Freezing the varieties with apple fiber also resulted in a reduction of viable bacterial cells from 0.8 log cfu g−1 in ice cream with L. paracasei and Lb. acidophilus to 1 log cfu g−1 in ice cream with L. casei, compared to the results after fermentation. The highest percentage overrun was determined in ice cream with L. paracasei and Lb. acidophilus. Ice cream with L. casei was characterized by significantly lower overrun on the 7th and 21st days of storage. Although L. paracasei ice cream had the highest overrun, it did not cause a significant reduction in the probiotic population during storage. After seven days of storage, the first drop differed significantly depending on the type of bacteria used for fermentation of the mixture and the addition of apple fiber. L. casei ice cream had a longer first drop time than L. paracasei and Lb. acidophilus ice cream. Partial replacement of inulin with apple fiber resulted in a significant darkening of the color of ice cream mixes. Depending on the type of bacteria used for fermentation, the addition of apple fiber decreased the value of the L* parameter. Ice cream mixes and ice cream with inulin and apple fiber were characterized by a high proportion of yellow. Partial replacement of inulin with apple fiber reduced the hardness of ice cream compared to inulin-only ice cream. Moreover, the panelists found that ice cream with inulin was characterized by a sweeter taste than ice cream with apple fiber. Moreover, the addition of apple fiber favorably increased the flavor and aroma perception of the mango-passion fruit. Therefore, the milk of Olkuska sheep could be successfully used for the production of symbiotic dairy ice cream.

scholarly journals Probiotic Fermented Milk with Collagen

Dairy ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. 126-134
Author(s):  
Agata Znamirowska ◽  
Katarzyna Szajnar ◽  
Małgorzata Pawlos
Keyword(s):  
Ph Value ◽  
Protein Hydrolysate ◽  
Lactobacillus Rhamnosus ◽  
Fermented Milk ◽  
Microbiological Analysis ◽  
Refrigerated Storage ◽  
Organoleptic Properties ◽  
Bifidobacterium Animalis ◽  
Collagen Protein ◽  
C 30

The aim of this study was to assess the possibility of using collagen protein hydrolysate in the production of milk fermented by Bifidobacterium animalis ssp. lactis Bb-12 and Lactobacillusrhamnosus. Physicochemical and organoleptic properties were studied and microbiological analysis of fermented milk was performed on days 1 and 21 of storage. Milk with the addition of 3% collagen protein hydrolysate was pasteurized (85 °C/30 min), divided into two groups, cooled to 37 °C and inoculated: the first group with Bifidobacteriumanimalis ssp. lactis Bb-12, second with Lactobacillus rhamnosus. Incubation was carried out at 37 °C/10 h. After 21 days of refrigerated storage, the pH value decreased in all analyzed milk samples. Collagen protein hydrolysate was a good milk additive to increase gel hardness and reduce syneresis. Moreover, its addition did not change the taste and odour of milk fermented with the use of Bifidobacterium Bb-12 and Lactobacillus rhamnosus. Collagen protein hydrolysate favourably stimulated the survival of Bifidobacterium Bb-12 during 21 days of storage. After 21 days of cold storage in milk with collagen, the number of Lactobacillus rhamnosus cells was reduced by 0.11 log cfu g−1.

scholarly journals Effect of Vitamin C Source on Its Stability during Storage and the Properties of Milk Fermented by Lactobacillus rhamnosus

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6187
Author(s):  
Agata Znamirowska ◽  
Katarzyna Szajnar ◽  
Małgorzata Pawlos
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Lactobacillus Rhamnosus ◽  
Experimental Period ◽  
Fermented Milk ◽  
Bacterial Cells ◽  
Organoleptic Evaluation ◽  
Growth And Survival ◽  
Texture Profile ◽  
Control Milk

The enrichment of commonly consumed foods with bioactive components might be helpful in promoting health and reducing the risk of disease, so the enrichment of probiotic fermented milk with vitamin C can be considered appropriate. The effect of vitamin C addition depends on the source of origin (rosehip, acerola and ascorbic acid in powder form) on the growth and survival of Lactobacillus rhamnosus and the quality of fermented milk on the 1st and 21st day of storage was analyzed. The pH, total acidity, vitamin C, syneresis, color, texture profile and numbers of bacterial cells in fermented milk were determined. The organoleptic evaluation was also performed. The degradation of vitamin C in milk was shown to depend on its source. The lowest reduction of vitamin C was determined in milk with rosehip. The least stable was vitamin C naturally found in control milk. The addition of rosehip and acerola decreased syneresis and lightness of milk color, increasing the yellow and red color proportion. In contrast, milk with ascorbic acid was the lightest during the whole experimental period and was characterized by a very soft gel. The growth of Lactobacillus rhamnosus during fermentation was most positively affected by the addition of rosehip. However, the best survival of Lactobacillus rhamnosus was demonstrated in milk with acerola. On the 21st day of storage, the number of L. rhamnosus cells in the control milk and the milk with vitamin C was >8 log cfu g−1, so these milks met the criterion of therapeutic minimum. According to the assessors, the taste and odor contributed by the addition of rosehip was the most intense of all the vitamin C sources used in the study.

Creation of a fermented milk product using the association of probiotic cultures

2021 ◽  
pp. 36-39
Author(s):  
Ирина Владимировна Рожкова ◽  
Анна Васильевна Бегунова ◽  
Юлия Игоревна Крысанова
Keyword(s):  
Salmonella Typhimurium ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Fermented Milk ◽  
Starter Cultures ◽  
Milk Product ◽  
Milk Products ◽  
Fermented Milk Product ◽  
Fermented Milk Products

Кисломолочные продукты приобретают популярность во всем мире благодаря их полезным и функциональным свойствам. Проблема разработки и широкого использования кисломолочных продуктов приобретает огромное значение в период пандемии и экологического кризиса. Полезные свойства кисломолочных продуктов зависят от состава заквасок, используемых для их производства. Вид и количество используемых в составе закваски штаммов обуславливает бифункциональный эффект пробиотического кисломолочного продукта. Из молочнокислых бактерий Lactobacillus являются наиболее изученными пробиотическими микроорганизмами с широким спектром физиолого-биохимических свойств. Для разработки кисломолочного продукта была использована ассоциация заквасочных культур, состоящая из Lactobacillus rhamnosus TR, Lactobacillus reuteri LR1 и Lactobacillus acidophilus H-9 в соотношении 1:6:1, которая обладает функциональным потенциалом. В работе научно обоснованы технологические режимы производства пробиотического кисломолочного продукта c использованием указанной выше ассоциации культур, которая обеспечивает сквашивание пастеризованного обезжиренного молока за 7-8 ч при внесении 5-7 % инокулята, обеспечивая определенные органолептические показатели. При этом количество клеток пробиотических культур составляет для L. acidophilus Н-9 1,1×10 КОЕ/см, для L. reuteri LR 1 (2,1±1,3)×10КОЕ/см, для L. rhamnosus TR (1,37±0,98)×10 КОЕ/см. Показано, что разработанный кисломолочный продукт обладает антагонистической активностью к S. aureus АТСС 6538 и Salmonella typhimurium NCTC 00074, а также антиоксидантной и АПФ-ингибирующей активностью, что характеризует его бифункциональные свойства. Диаметр зоны ингибирования роста S. aureus АТСС 6538 составляет 15,25±0,35 см, а Salmonella typhimurium NCTC 00074 10,5±0,71 см. Антиоксидантная активность разработанного продукта составляет 570,64±18 мкмоль TЭ/мг, а АПФ ингибирующая активность - IC 1,23±1,6 мг белка/см. Fermented milk products are gaining popularity all over the world due to their useful and functional properties. The problem of the development and widespread use of fermented milk products becomes of great importance during a pandemic and an ecological crisis. The beneficial properties of fermented milk products depend on the composition of the starter cultures used for their production. The type and quantity of strains used in the starter culture determine the bifunctional effect of the probiotic fermented milk product. Of the lactic acid bacteria, Lactobacillus is the most studied probiotic genus with a wide range of physiological and biochemical properties. For the development of a fermented milk product, an association of starter cultures was used, consisting of Lactobacillus rhamnosus TR, Lactobacillus reuteri LR1, and Lactobacillus acidophilus H-9 in a ratio of 1: 6:1, which has a functional potential. The work scientifically substantiates the technological modes of production of a probiotic fermented milk product using the above-mentioned association of cultures, which ensures the fermentation of pasteurized skim milk in 7-8 hours with the introduction of 5-7 % inoculum, providing certain organoleptic characteristics. In this case, the cells number of probiotic cultures for L. acidophilus H-9 is 1.1×10 CFU/cm, for L. reuteri LR 1 (2.1±1.3)×10 CFU/cm, for L. rhamnosus TR (1.37±0.98)×10 CFU/cm. It was shown that the developed fermented milk product has an antagonistic activity to S. aureus ATCC 6538 and Salmonella typhimurium NCTC 00074, as well as antioxidant and ACE-inhibiting activity, which characterizes its bifunctional properties. The diameter of the growth inhibition zone for S. aureus ATCC 6538 is 15.25±0.35 cm and for Salmonella typhimurium NCTC 00074 10.5±0.71 cm. The antioxidant activity of the developed product is 570.64±18 μmol TE/mg, and ACE inhibitory activity IC50 1.23±1.6 mg protein/cm.

scholarly journals Digestão Simulada de Bebida Esportiva Contendo Diferentes Probióticos

2020 ◽  
Vol 24 (1) ◽  
pp. 47-53
Author(s):  
Luciana Carvalho Fino ◽  
Fabíola Lima Pena ◽  
Maria Carolina Pelatieri Valle ◽  
Thais Ramos Silva ◽  
Vivian Cristina Cruz Rodrigues ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Lactobacillus Rhamnosus ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Pomegranate Juice ◽  
Sports Drink ◽  
Bifidobacterium Animalis

O exercício físico de alta intensidade está relacionado com diversas alterações sistêmicas que podem prejudicar a performance do indivíduo, tais como: a queda da imunidade, a perda de massa muscular, a insônia, entre outros. Os probióticos, quando administrados regularmente para esse público, podem reduzir os sintomas relacionados ao overtraining. O presente estudo buscou selecionar uma cepa probiótica para empregar em uma bebida esportiva (a base de leite fermentado), contendo elevada quantidade de compostos fenólicos e adicionada de cepas probióticas, sendo utilizadas como principal critério de escolha a sobrevivência da mesma após digestão simulada. Foram avaliados os probióticos Lactobacillus casei (BGP93), Lactobacillus rhamnosus (SP1) e Lactobacillus acidophilus (LA3) e Bifidobacterium animalis subsp. lactis (BB12), utilizando-se como cultura starter o Streptococcus thermophilus (TA072). Em paralelo foi realizada avaliação da resistência das culturas probióticas em solução adicionada de suco de romã, tendo em vista a potencial atividade antimicrobiana desta fruta. Neste teste foi observado que as culturas probióticas não foram inibidas pelo suco de romã, com exceção do L. acidophilus LA3. Para digestão in vitro foi utilizado protocolo validado composto pelas fases gástrica e entérica, a viabilidade dos probióticos na bebida esportiva foi acompanhada durante armazenamento refrigerado a 6 °C por 28 dias e a sobrevivência foi avaliada, por meio de contagem seletiva em DeMan, Rogosa & Sharpe (MRS) ágar acidificado e incubação em anaerobiose. Os resultados demonstraram que o Lactobacillus casei cepa BGP93 apresentou adequada viabilidade durante o armazenamento e maior taxa de sobrevivência à digestão, quando comparado aos demais probióticos, representando um bom candidato para a elaboração da bebida esportiva. Palavras chave: Leite Fermentado. Compostos Fenólicos. Alimento Funcional. Viabilidade de Probióticos.    Abstract High-intensity physical exercise is related to several systemic alterations that may impair the individual's performance, such as immunity depletion, muscle mass loss, insomnia, among others. Probiotics, when given regularly to this public, may reduce symptoms related to overtraining. The present study aimed to select a probiotic strain for use in a sports drink (based on fermented milk) containing a high amount of phenolic compounds, and added probiotic strains, being the main criterion of choice the survival of the strain after simulated digestion. The probiotics Lactobacillus casei (BGP93), Lactobacillus rhamnosus (SP1) and Lactobacillus acidophilus (LA3) and Bifidobacterium animalis subsp. lactis (BB12), using as starter culture Streptococcus thermophilus (TA072). At the same time, resistance evaluation of probiotic cultures in pomegranate juice solution was carried out, considering the potential antimicrobial activity of this fruit. In this test, it was observed that probiotic cultures were not inhibited by pomegranate juice, except for L. acidophilus LA3. For in vitro digestion a validated protocol consisting of gastric and enteric phases was used, the viability of probiotics in the sports drink was monitored during cold storage at 6 ° C for 28 days and survival was evaluated by selective counting in acidified DeMan, Rogosa & Sharpe (MRS) agar and incubation in anaerobiosis. The results showed that Lactobacillus casei strain BGP93 showed adequate viability during storage and higher survival rate to digestion when compared to other probiotics, representing a good candidate for the sport drink preparation Keywords: Fermented Milk. Phenolic Compounds. Functional Food. Probiotics Viability.

scholarly journals Microbiological detection of probiotic microorganisms in fermented milk products

2007 ◽  
Vol 55 (2) ◽  
pp. 15-20 ◽  
Author(s):  
Radka Burdychová
Keyword(s):  
Lactobacillus Acidophilus ◽  
Health Benefits ◽  
Lactobacillus Rhamnosus ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Culture Conditions ◽  
Probiotic Bacteria ◽  
Potential Health ◽  
Milk Products ◽  
Fermented Milk Products

A number of health benefits have been claimed for probiotic bacteria such as Lactobacillus acidophilus, Bifidobacterium spp. and Lactobacillus rhamnosus. Because of the potential health benefits, these organisms are increasingly incorporated into dairy foods. However, to reach health benefits, the concentration of probiotics have to be 106 CFU/g of a product. For assessing of required probiotic bacteria quantity, it is important to have a working method for selective enumeration of these probiotic bacteria. Five bacteriological media were evaluated to assess their suitability to selectively enumerate Streptococcus thermophilus, Lactobacillus rhamnosus, Lactobacillus acidophilus and Bifidobacterium spp. Bacteriological media evaluated included Streptococcus thermophilus agar, pH modified MRS agar, MRS-vancomycine agar and BSM (Bifidus selective medium) agar under different culture conditions.Seven selected fermented milk products with probiotic culture were analyzed for their bacterial populations using the described selective bacteriological media and culture conditions. All milk products contained probiotic microorganisms claimed to be present in declared quantity (106–107/g).

scholarly journals Studies on Development of Fibre Rich Probiotic Frozen Yogurt

2021 ◽  
Author(s):  
S.D. Katke ◽  
H.W. Deshpande
Keyword(s):  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Health Benefits ◽  
Buffalo Milk ◽  
Global Market ◽  
Consumer Acceptability ◽  
Organoleptic Evaluation ◽  
Soluble Fibre ◽  
Psyllium Husk ◽  
Frozen Yogurt

Background: Yogurt is one of the most popular fermented dairy products worldwide which has great consumer acceptability due to its health benefits other than its basic nutrition. Yogurt is a rich source of calcium in bio-available form and also acts as a probiotic carrier food. Yogurt is reported to be beneficial for the treatment of Inflammatory Bowel Disease (IBD) that includes gastrointestinal disorders. Because of these known health benefits of yogurt, consumer demand for yogurt and yogurt related products has been increased and became the fastest growing dairy category in the global market. Notably, India ranks first in psyllium husk (isabgol) production and is the sole supplier of seeds and husk in the international market. Among medicinal plants, psyllium is the first ranked foreign exchange earner for the country. India is the largest producer and the main supplier of seed and husk to the world market. The psyllium is high in soluble fibre content with detoxing effect over digestive system makes it a very apt nutraceutical. Methods: The present investigation indicates utilization of acid modified psyllium husk for the preparation of probiotic frozen yogurt. The prepared probiotic frozen yogurt was analyzed for sensorial, physicochemical and microbial quality parameters. Probiotic frozen yogurt was prepared from 1 liter buffalo milk, 10 gm sugar and 0.5 gm of acid modified psyllium husk (0.60% HCl in the ethanol solvent with ratio of 1:7). The probiotic culture (107, 108, 109 cfu/gm) containing equal proportions of Lactobacillus acidophilus and Lactobacillus plantarum were added in encapsulated form. It was then stored at refrigerated conditions at 4°C for 8 hours. Result: The organoleptic evaluation of probiotic frozen yogurt was carried out. As per the score of 9-point hedonic scale, probiotic frozen yogurt prepared with 10 per cent encapsulated probiotic culture (109 cfu/gm and 5 per cent each of Lactobacillus acidophilus and Lactobacillus plantarum) and 0.5 gm of acid modified psyllium husk had shown maximum consumer acceptability (8.7) among all samples.

scholarly journals Effect of Calcium Compound Type and Dosage on the Properties of Acid Rennet Goat’s Milk Gels

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5563
Author(s):  
Małgorzata Pawlos ◽  
Agata Znamirowska ◽  
Katarzyna Szajnar
Keyword(s):  
Calcium Carbonate ◽  
Ph Value ◽  
Control Sample ◽  
Organoleptic Evaluation ◽  
Texture Profile ◽  
Goat’S Milk ◽  
Milk Fermentation ◽  
Compound Type ◽  
Goat's Milk ◽  
Calcium Compounds

The aim of this study was to determine the effect of adding calcium compounds to processed goat’s milk, and on the properties of acid rennet goat’s milk gels, which are a middle product obtained in the manufacture of acid rennet cheese. The properties of the gels directly affect the quality of acid rennet cheeses. The analysis of raw goat’s milk was carried out, then acid rennet gels were produced with the addition of six different calcium compounds (chloride, citrate, bisglycinate, gluconate, lactate, and carbonate). The dynamics of milk fermentation were performed by monitoring the pH value of milk during acidification. The pH, syneresis, color, and texture profile were determined in the formulated acid rennet gels. An organoleptic evaluation was also performed. The study demonstrated that, not only calcium chloride, but also calcium citrate, gluconate, lactate, bisglycinate, and calcium carbonate could be used in the production of goat’s milk acid rennet gels, or the middle product in the manufacture of acid rennet curd cheese from goat’s milk. Notably, the addition of citrate, bisglycinate, and calcium carbonate in doses of 20 mg Ca 100 g−1 most effectively reduced syneresis compared to the control sample by 4.76% (citrate), 7.85% (bisglycinate), and 10.28% (carbonate). The hardness of the control gels ranged from 2.35 N to 2.99 N. The addition of chloride, citrate, gluconate, lactate, and calcium carbonate to the milk improved the acid rennet gel’s hardness. The addition of 20 mg Ca 100 g−1 as gluconate increased the hardness the most (3.61 N). When increasing the calcium dosage in the form of all compounds, there was a tendency to increase the gel’s springiness. The addition of chloride, citrate, and bisglycinate to milk did not result in a darkening of the gel’s color. The addition of calcium compounds mostly reduced the intensity of goatish taste and odor. Calcium gluconate, in particular, reduced the goatish taste the most, a taste which is not always acceptable by the consumers.

scholarly journals Development of kefir from non-traditional raw materials enriched with blackberry products

2021 ◽  
Vol 32 ◽  
pp. 03001
Author(s):  
Alexey Belyaev ◽  
Vladimir Mosyagin ◽  
Galina Ryzhkova ◽  
Olga Shvets
Keyword(s):  
Raw Materials ◽  
Control Sample ◽  
Fermented Milk ◽  
The Body ◽  
Normal Operation ◽  
Milk Product ◽  
Organoleptic Evaluation ◽  
Goat’S Milk ◽  
Goat's Milk ◽  
Materials Used

The article presents data on the development of a new fermented milk product kefir from unconventional milk raw materials of goat’s milk and with the addition of blackberry vegetable additive. It is scientifically justified to use blackberry puree in the production technology, as well as the use of an unconventional type of milk for the manufacture of kefir. The influence of blackberries on the quality indicators of the developed kefir was also investigated. Comparative analysis of kefir with different concentrations of blackberries was carried out. According to the results of organoleptic evaluation, it was revealed that the consistency in all samples is uniform, without disturbing the clot. The taste is sour milk, slightly sharp, without extraneous flavors and odors. There is a good slightly sweet blackberry taste and smell with an increase in the concentration of the additive. It can also be noted from the results that kefir with blackberries is enriched with iron and other micro and macro elements in the comparison with the control sample. According to physicochemical indicators, the sample with 10% replacement of milk with mashed potatoes was identified as the closest to the control. The benefit of goat’s milk kefir is due to the presence of various substances necessary for life. The properties of raw materials used are preserved and even enhanced considering the use of fermentation. Such kefir has a lot of natural proteins, the product is easily digested and absorbed by the body. Goat’s kefir makes it possible to improve digestion and metabolic processes in regular consumption. This product reduces the risk of developing most intestinal infections because of rich composition. There are probiotics in such kefir that oppose the spread of harmful microflora in the intestine. The product includes potassium and magnesium minerals that are especially important for normal operation of heart and vessels. There is phosphorus and calcium in it. The joint action of these minerals activates the process of regeneration and strengthening of bone tissue.

scholarly journals Technological properties of Lactobacillus rhamnosus K3 isolated from fermented cabbage and its potential use as starter culture for fermented food products

2019 ◽  
Vol 121 (4) ◽  
pp. 89-101
Author(s):  
Anna Łepecka ◽  
Dorota Zielińska ◽  
Monika Brejnak ◽  
Aleksandra Ołdak ◽  
Danuta Kołożyn-Krajewska
Keyword(s):  
Bacterial Strain ◽  
Ph Value ◽  
Starter Culture ◽  
Lactobacillus Rhamnosus ◽  
Fermented Food ◽  
Bacterial Cells ◽  
Technological Properties ◽  
Biochemical Tests ◽  
Sugar Fermentation ◽  
Milk Fermentation

The objective of the research study was to specify technological properties of the Lactobacillus rhamnosus K3 strain. This bacterial strain was tested for its ability to grow under the processing conditions, such as temperature [ºC]: 10, 15, 45, pH value: 3.9, 6.4, 9.6, and a high NaCl concentration [%]: 5, 8, 10. Biochemical tests (sugar fermentation and enzyme activity) were carried out and the survival of those bacteria was assayed in the medium depending on the type of food (milk, tomato juice, and beef broth). Milk fermented with Lb. rhamnosus K3 was subjected to 6-week incubation. The results showed that the Lb. rhamnosus K3 strain was able to grow at different temperatures but within a narrow pH range. The bacterial strain did not tolerate high NaCl concentrations, however it grew well in any kind of food medium. A particularly good bacterial growth was observed in milk. Lb. rhamnosus K3 was able to metabolize sugars. This strain did not reduce nitrates; no catalase activity was detected either. The β-galactosidase enzyme was identified. The best temperature of milk fermentation was proved to be 37 ºC. It was also found that Lb. rhamnosus K3 had functional properties allowing its use as a starter culture for milk. The number of bacterial cells remained at a level higher than 8 log CFU/ml throughout the entire 6-week incubation of refrigeration storage (4 ºC). The bacteria of Lactobacillus rhamnosus K3 were capable of fermenting milk confirming their suitability as a starter culture for milk products.

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