scholarly journals Chemical Composition and Larvicidal Activity of Lavandula angustifolia Subsp. angustifolia and Lavandula dentata Spp. dentata Essential Oils against Culex pipiens Larvae, Vector of West Nile Virus

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Fouad El-Akhal ◽  
Amal Ramzi ◽  
Abdellah Farah ◽  
Yassine Ez Zoubi ◽  
Moussa Benboubker ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Culex Pipiens ◽  
Mosquito Control ◽  
World Health ◽  
Environmental Damage ◽  
Probit Regression ◽  
Lavandula Angustifolia ◽  
Health Organization ◽  
Lc50 And Lc90

The Culex pipiens mosquito (Diptera: Culicidae) is highly suspected to be the vector responsible for the spread of several parasitic and viral diseases. The use of synthetic insecticides is generally the preferred method of controlling these mosquitoes’ proliferation. However, it has led to resistance problems in target mosquitoes and environmental damage. Hence, diverse plant extracts could be considered as an alternative and potential source as mosquito control agents. In this study, essential oils of Lavandula angustifolia subsp. angustifolia and Lavandula dentata spp. dentata that are growing in Morocco were examined for their insecticidal effects on Culex pipiens larvae. The bioassay was performed according to a methodology inspired by the standard protocol of the World Health Organization. The mortality rate was determined after 24 hours of exposure, and probit regression analysis was used to calculate LC50 and LC90. The chemical analysis revealed that the principal compounds of L. angustifolia subsp. essential oils include linalool, linalyl acetate, geraniol, lavandulyl acetate, camphor, β-caryophyllene, terpinen-4-ol, β-myrcene, and 1,8-cineole, while the essential oil of L. dentata spp. was mainly composed of 1,8-cineole, camphor, α-pinene, trans-pinocarveol, linalool, and borneol. These volatile compounds have shown a toxic effect against Culex pipiens larvae, with lethal concentrations LC50 and LC90 being, respectively, 140 µg/ml and 450 µg/ml, for the L. angustifolia subsp. essential oil. Meanwhile, they were estimated at 2670 µg/ml and 7400 µg/ml, respectively, for the L. dentata spp. essential oil. These results suggest using essential oils of two species of Lavandula to control the Culex pipiens mosquito. It could be useful for the study of new natural larvicidal compounds.

scholarly journals Chemical composition and insecticidal activity of Anacyclus pyrethrum essential oil from the Bensliman area against Culex pipiens

2020 ◽  
Vol 10 (1) ◽  
pp. 13-21
Author(s):  
Kawtar El Mokhtari ◽  
M'hammed EL Kouali ◽  
Mohammed Talbi ◽  
Latifa Hajji ◽  
Abdelhak El Brouzi
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Insecticidal Activity ◽  
Culex Pipiens ◽  
Larval Mortality ◽  
World Health ◽  
Herbaceous Plant ◽  
Health Organization ◽  
Asteraceae Family ◽  
Lc50 And Lc90

Anacyclus pyrethrum is a herbaceous plant that belongs to the Asteraceae family. The focus of the present study is to extract the essential oil from this plant, to determine its chemical composition and to evaluate its insecticidal activity against the larvae of the mosquito Culex pipiens. The essential oil from A. pyrethrum collected from the region of Bensliman in Morocco was extracted by hydrodistillation and analyzed by gas chromatography coupled to mass spectrometry were studied. Essential oil yield obtained by hydrodistillation was 0.09%. The major aroma constituents were Spathulenol (20.47%), Germacrene D (16.48%), Caryophyllene oxide (13.20%), 4(14)-Salviale-1-one (8.27%) and Caryophyllene 4(14),8(15)-dien-5α-ol (7.30%). The larvicidal test carried out according to a methodology based on the standard protocol of the World Health Organization was studied on 4th instar larvae of C. pipiens and showed that A. pyrethrum essential oil possesses remarkable insecticidal properties.  After 24 hours of exposition, larvicidal assays revealed a 100% mortality of C. pipiens larvae. The dose of 40 μL/mL was toxic enough to cause 100% larval mortality of C. pipiens. The lethal concentrations LC50 and LC90 calculated for the essential oil studied were of the order of 14.79 μL/mL and 19.95 μL/mL, respectively. To control mosquitoes, this essential oil extracted from A. pyrethrum might be used as a natural insecticide and therefore could be an alternative to synthetic insecticides already present on the market.

scholarly journals Chemical Composition and Larvicidal Activity of Mentha pulegium ssential Oil from Central Morocco Against Larvae of Mosquito Culex pipiens (Diptera: Culicidae)

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Fouad El-Akhal ◽  
Yassine Ezzoubi ◽  
Taghzouti Khalid ◽  
Abdelhakim El Ouali Lalami
Keyword(s):  
Essential Oil ◽  
Larvicidal Activity ◽  
Culex Pipiens ◽  
Mosquito Species ◽  
Probit Analysis ◽  
World Health ◽  
Mentha Pulegium ◽  
The North ◽  
Medicinal Plant Extracts ◽  
Lc50 And Lc90

Background: In Morocco, the species Culex pipiens (Diptera: Culicidae) has developed resistance to the synthetic insecticide. Therefore, there is an urgent need to find alternatives to the insecticides, as natural herbal biocides. Objective: The essential oil of Mentha pulegium grown the North center of in Morocco has been analyzed and their larvicidal activity on Culex pipiens was determined. Methods: The analysis and the identification of the various constituents of essential oilobtained by hydro-distillation of the aerial part were carried out by Gas Chromatography coupled with Mass Spectrometry (GC/MS). Biological tests were realized according to a methodology inspired from standard World Health Organization protocol. The larvicidal activity of medicinal plant extracts were tested against early fourth-instar larvae of Culex pipiens. The repellent efficacy was determined against of mosquito species (Culex pipiens) at six concentration’s (5, 10, 20, 40, 80 and 160 ppm) under the laboratory condition. The mortality was observed 24h after treatment; data were subjected to probit analysis to determine the Lethal Concentrations (LC50 and LC90) to kill 50 and 90 percent of the treated larvae of tested species. Results: The major constituents of Mentha pulegium essential oil were identified as pulegone (53.23%), α-pinene (12.1%), piperitone (9.62%), menthone (9.26) and piperitenone (6.6%). The LC50 and LC90 were estimated at 25.45 ppm and 98.66 ppm respectively. Conclusion: This investigation indicates that the Mentha pulegium essential oil could serve as a potential larvicidal and could be used as an ideal ecofriendly approach for the control of the Culex pipiens.

Chemical Profiling, Larvicidal Activity and Antihemolytic Property of Allium sativum L. and Allium cepa L. Essential Oil

2021 ◽  
Vol 23 (09) ◽  
pp. 945-958
Author(s):  
Durgadevi P ◽  
◽  
Sumathi P ◽  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Allium Cepa ◽  
Larvicidal Activity ◽  
Allium Sativum ◽  
World Health ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Health Organization

Essential oils are plant derived concentrates of the secondary metabolites responsible for the aromatic flavor attributing to its various medicinal properties. Fresh Allium sativum (A. sativum) and Allium cepa (A. cepa) were subjected to steam distillation for isolation of essential oil characterized by performing Gas Chromatography – Mass Spectroscopy (GC-MS). Chromatogram of the essential oil depicted the presence diallyl sulfide (5.35%), 2-(2’-carbamoylphenoxy)-butanoic acid (2.64%), 2-ethyl-5-methylthiophene (0.42%), diallyl disulphide (18.76%), 3-(2-thia-4-pentenyl)-1-thia-cyclohex-5-ene (1.09%) and dimethyl tetrasulphide (0.15%), 2,4-dimethylpyrido[2,3-d]pyrimidin-5-one (47.91%), 2,4-Thiazolidinedione (0.01%), 5-chloro-2-hydroxy-1,3-dinitrobenzene (5.93%), 6-Methoxy-1-methyl-3,4-dihydroisoquinoline (47.91%) in A. sativum and A.cepa respectively. Larvicidal activity against third instar larvae of Anopheles stephensi (A. Stephensi) was assessed by following the standard protocol of World Health Organization. The 50% lethality (LC50) of A. stephensi larvae was observed at 265.96 ± 1.88 ppm and 357.14 ± 2.36 ppm of A. sativum and A. cepa essential oil correspondingly. The mortality rate of the larvae was both time and dose dependent. Besides, the in vitro antihemolytic activity of the essential oil was also assessed using Sheep erythrocytes. The erythrocyte lysis was inhibited by the essential oils of both A. sativum and A. cepa in a concentration dependent manner with an IC50 of 427.35 ± 1.23 μl and 549.45 ± 1.38 μl respectively. On a comparative assessment between the essential oils of A. sativum and A. cepa, the former exhibited better larvicidal activity against the disease-causing vector, A. stephensi. Still, both could serve as potent insecticidal agents after further identification of the responsible chemical compound and its mode of action.

scholarly journals Development of nano-emulsions based on Ayapana triplinervis for the control of Aedes aegypti larvae

2020 ◽  
Author(s):  
Alex Bruno Lobato Rodrigues ◽  
Rosany Martins Lopes ◽  
Érica Menezes Rabelo ◽  
Rosana Tomazi ◽  
Lizandra Lima Santos ◽  
...  
Keyword(s):  
Particle Size ◽  
Essential Oil ◽  
Essential Oils ◽  
Dimethyl Ether ◽  
Inflammatory Cells ◽  
World Health ◽  
Oral Toxicity ◽  
Religious Rituals ◽  
Health Organization ◽  
Nano Emulsion

AbstractAyapana triplinervis is a plant species used in traditional medicine and in mystical-religious rituals by traditional communities in the Amazon. The aim of this study is to evaluate the larvicidal activity against A. aegypti of nano-emulsions containing essential oils from A. triplinervis morphotypes, and acute oral toxicity in non-target organism. Essential oils were identified and nano-emulsions were prepared using the low energy method. The mortality test of A. aegypti larvae was performed according to the protocol recommended by the World Health Organization, and toxicity in non-target mammals was performed according to the OECD. Phytochemical analyses indicated the major compounds (E)-Caryophyllene (45.93%) and Thymohydroquinone Dimethyl Ether (32.93%) in morphotype A, and in morphotype B, Thymohydroquinone Dimethyl Ether (84.53%) was found. Morphotype A essential oil nano-emulsion showed a particle size of 101.400 ± 0.971 nm (PdI = 0.124 ± 0.009 and ZP = −19,300 ± 0.787 mV). Morphotype B essential oil nano-emulsion had a particle size of 104.567 ± 0.416 nm (PdI = 0.168 ± 0.016 and ZP = −27,700 ± 1,307 mV). Histomorphological analyses showed the presence of inflammatory cells in the liver of animals treated with morphotype A essential oil nano-emulsion (MAEON) and morphotype B essential oil nano-emulsion (MBEON). Congestion and the presence of transudate with leukocyte infiltration in the lung of animals treated with MAEON were observed. The nano-emulsions containing essential oils of A. triplinervis morphotypes showed an effective nanobiotechnological product in the chemical control of A. aegypti larvae and safe for non-target mammals.

scholarly journals Assessment of the susceptibility status of Aedes aegypti (Diptera: Culicidae) populations to pyriproxyfen and malathion in a nation-wide monitoring of insecticide resistance performed in Brazil from 2017 to 2018

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kauara Brito Campos ◽  
Ademir Jesus Martins ◽  
Cynara de Melo Rodovalho ◽  
Diogo Fernandes Bellinato ◽  
Luciana dos Santos Dias ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Mosquito Control ◽  
Natural Populations ◽  
Adult Emergence ◽  
World Health ◽  
Two Generations ◽  
Wide Scale ◽  
Susceptibility Tests ◽  
The One ◽  
Health Organization

Abstract Background Chemical mosquito control using malathion has been applied in Brazil since 1985. To obtain chemical control effectiveness, vector susceptibility insecticide monitoring is required. This study aimed to describe bioassay standardizations and determine the susceptibility profile of Ae. aegypti populations to malathion and pyriproxyfen, used on a national scale in Brazil between 2017 and 2018, and discuss the observed impacts in arbovirus control. Methods The diagnostic-doses (DD) of pyriproxyfen and malathion were determined as the double of adult emergence inhibition (EI) and lethal doses for 99% of the Rockefeller reference strain, respectively. To monitor natural populations, sampling was performed in 132 Brazilian cities, using egg traps. Colonies were raised in the laboratory for one or two generations (F1 or F2) and submitted to susceptibility tests, where larvae were exposed to the pyriproxyfen DD (0.03 µg/l) and adults, to the malathion DD determined in the present study (20 µg), in addition to the one established by the World Health Organization (WHO) DD (50 µg) in a bottle assay. Dose-response (DR) bioassays with pyriproxyfen were performed on populations that did not achieve 98% EI in the DD assays. Results Susceptibility alterations to pyriproxyfen were recorded in six (4.5%) Ae. aegypti populations from the states of Bahia and Ceará, with Resistance Ratios (RR95) ranging from 1.51 to 3.58. Concerning malathion, 73 (55.3%) populations distributed throughout the country were resistant when exposed to the local DD 20 µg/bottle. On the other hand, no population was resistant, and only 10 (7.6%) populations in eight states were considered as exhibiting decreased susceptibility (mortality ratios between 90 and 98%) when exposed to the WHO DD (50 µg/bottle). Conclusions The feasibility of conducting an insecticide resistance monitoring action on a nation-wide scale was confirmed herein, employing standardized and strongly coordinated sampling methods and laboratory bioassays. Brazilian Ae. aegypti populations exhibiting decreased susceptibility to pyriproxyfen were identified. The local DD for malathion was more sensitive than the WHO DD for early decreased susceptibility detection.

scholarly journals Callicarpa Species from Central Vietnam: Essential Oil Compositions and Mosquito Larvicidal Activities

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 113 ◽  
Author(s):  
Nguyen Huy Hung ◽  
Le Thi Huong ◽  
Nguyen Thanh Chung ◽  
Nguyen Thi Hoai Thuong ◽  
Prabodh Satyal ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Mosquito Control ◽  
Control Agent ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Central Vietnam ◽  
Two Samples ◽  
Potential Alternative ◽  
Larvicidal Activities

There are around 140 species in the genus Callicarpa, with 23 species occurring in Vietnam. The Vietnamese Callicarpa species have been poorly studied. In this work, the leaf essential oils of C. bodinieri, C. candicans, C. formosana, C. longifolia, C. nudiflora, C. petelotii, C. rubella, and C. sinuata, have been obtained from plants growing in central Vietnam. The chemical compositions of the essential oils were determined using gas chromatography – mass spectrometry. Mosquito larvicidal activities of the essential oils were carried out against Aedes aegypti. All of the Callicarpa leaf essential oils showed larvicidal activity, but two samples of C. candicans were particularly active with 48-h LC50 values of 2.1 and 3.8 μg/mL. Callicarpa candicans essential oil should be considered as a potential alternative mosquito control agent.

scholarly journals Pyrethroid Resistance Status of Aedes (Stegomyia) Aegypti (Linneaus) from Dengue Endemic Areas in Peninsular Malaysia

2020 ◽  
Vol 16 (2) ◽  
Author(s):  
Rosilawati R ◽  
Lee HL ◽  
Nazni WA ◽  
Nurulhusna AH ◽  
Roziah A ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Pyrethroid Resistance ◽  
Resistance Management ◽  
Laboratory Strain ◽  
Control Agent ◽  
Peninsular Malaysia ◽  
World Health ◽  
Resistance Level ◽  
Health Organization

Vector control is still the principal method to control dengue and chemical insecticides, especially the pyrethroids such as permethrin are the forerunners of mosquito control agent. Intensive and extensive use of pyrethroids often result in resistance, thereby hampering control efforts. The present study was conducted to evaluate the susceptible status of Aedes aegypti, the primary vector of dengue against permethrin. A nationwide mosquito sampling via ovitrapping was conducted in 12 dengue hotspots across 5 states in Peninsular Malaysia. Field collected Aedes eggs were hatched and reared until L3 larval and further identified it species. Adult F0 Aedes aegypti were reared until F1 progeny and the female were used in adult assay, performed according to World Health Organization (WHO) protocol as to determine the resistance level. The laboratory strain maintained for more than 1000 generations that were susceptible to permethrin served as the control strain. Evaluation of resistance ratio was assessed by comparing the knockdown rate with laboratory susceptible strain. In this present study, 70% ofAe. aegypti population from dengue hotspots was highly resistance to permethrin. The study clearly demonstrated that widespread of permethrin resistant Ae. aegypti in Malaysian mosquito’s population, indicating the need of implementing an efficient pyrethroid resistance management.

scholarly journals Herbicidal potential of lavender (Lavandula angustifolia Mill.) essential oil components on bristly foxtail (Setaria verticillata (L.) P. Beauv.): Comparison with carvacrol, carvone, thymol and eugenol

2020 ◽  
Vol 72 (2) ◽  
pp. 223-231
Author(s):  
Katerina Koiou ◽  
Ioannis Vasilakoglou ◽  
Kico Dhima
Keyword(s):  
Essential Oil ◽  
Synergistic Effect ◽  
Essential Oils ◽  
Weed Control ◽  
Root Length ◽  
Linalyl Acetate ◽  
Lavandula Angustifolia ◽  
Natural Herbicides ◽  
Setaria Verticillata ◽  
Oil Components

Essential oils are a plentiful source of plant compounds for potential use in the development of natural herbicides. With this in mind, the phytotoxicity of ten major essential oil components of lavender (Lavandula angustifolia Mill.) on the weed species bristly foxtail (Setaria verticillata (L.) P. Beauv.) was determined using a perlite-based Petri-dish bioassay. Their phytotoxicity was also compared with that of well-known phytotoxic essential oil components (carvacrol, thymol, carvone and eugenol) of oregano (Origanum vulgare L.) and clove (Syzygium aromaticum (L.) Merr. & L.M. Perry) essential oils. Potential synergistic or antagonistic effects between carvacrol or eugenol with other components of lavender essential oil were investigated. Regarding the most phytotoxic components, terpinen-4-ol at 80 nL/cm3 completely inhibited the germination and root length of bristly foxtail, displaying similar phytotoxicity to carvone and thymol. Like carvacrol, lavandulol and linalyl acetate caused total (100%) germination and root length reduction of bristly foxtail at 160 nL/cm3, while the same effect was achieved by lavandulyl acetate at 320 nL/cm3. A synergistic effect was also observed when carvacrol or eugenol were combined with ocimene, 3-octanone, ?-terpineol or terpinen-4-ol. Focusing on the development of alternative weed control strategies, lavender essential oils containing high concentrations of terpinen-4-ol, lavandulol or linalyl acetate could be useful for the production of natural herbicides. These essential oil components combined with selected oregano or clove essential oil components, increase phytotoxicity and weed control due to the synergistic effect observed when in mixture.

scholarly journals Contribution to the Analysis of the Volatile Constituents from some Lavender and Lavandin Cultivars Grown in Greece

2003 ◽  
Vol 71 (3) ◽  
pp. 229-234 ◽  
Author(s):  
Paschalina Chakopoulou ◽  
Apostolos Goliaris ◽  
S. Katsiotis
Keyword(s):  
Essential Oil ◽  
Qualitative Analysis ◽  
Essential Oils ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Lavandula Angustifolia ◽  
Volatile Constituents

The essential oils of Lavandula angustifolia and the Lavandin hybrids "super" and "special" grown in Greece, were studied in order to evaluate their quality as far as the correlation of their essential oil composition. The essential oil yields were determined to 4.4%, 7.5% and 8.5% in the lavandula and lavandin "super" and "special" hybrids respectively. Fifty-nine constituents were identiiied representing the 99% of the oils. The qualitative analysis of the oils was carried out by GCIMS and revealed that linalool and linalylacetate were the predominant constituents. Remarkable differences were noticed between the camphor percentages among them (11.35% and 5.03% for the "super " and "special" respectively).

scholarly journals Molluscicidal and Mosquitocidal Activities of the Essential oils of Thymus capitatus Hoff. et Link. and Marrubium vulgare L.

2012 ◽  
Vol 54 (5) ◽  
pp. 281-286 ◽  
Author(s):  
Maha M. Salama ◽  
Eman E. Taher ◽  
Mohamed M. El-Bahy
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Gas Chromatography Mass Spectrometry ◽  
Ovicidal Activity ◽  
Oil Composition ◽  
Mosquitocidal Activity ◽  
Two Samples ◽  
Thymus Capitatus ◽  
Marrubium Vulgare ◽  
Lc50 And Lc90

Steam distillation of essential oils of aerial parts of Thymus capitatus and Marrubium vulgare L. collected at North cost of Egypt yielded 0.5% and 0.2%, respectively. Results of Gas chromatography-mass spectrometry analyses of the two samples identified 96.27% and 90.19% of the total oil composition for T. capitatus and M. vulgare, respectively. The two oil samples appeared dominated by the oxygenated constituents (88.22% for T. capitatus and 57.50% for M. vulgare), composed of phenols, mainly carvacrol (32.98%) and thymol (32.82%) in essential oil of T. capitatus, and thymol (34.55%) in essential oil of M. vulgare. It was evaluated the molluscicidal activity of T. capitatus and M. vulgare essential oils on adult and eggs of Biomphalaria alexandrina as well as their mosquitocidal activity on Culex pipiens. The LC50 and LC90 of T. capitatus essential oil against adult snails was 200 and 400 ppm/3hrs, respectively, while for M. vulgare it was 50 and 100 ppm/3hrs, respectively. Moreover, M. vulgare showed LC100 ovicidal activity at 200 ppm/24 hrs while T. capitatus oil showed no ovicidal activity. It was verified mosquitocidal activity, with LC50 and LC90 of 100 and 200 ppm/12hrs respectively for larvae, and 200 and 400 ppm/12hrs respectively for pupae of C. pipiens.

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