scholarly journals Genetic Variation of Glutathione S-Transferase M1 Is Associated with Patients with Ovarian Endometriosis and Endometriosis-Related Primary Infertility

2021 ◽  
pp. 1-6
Author(s):  
Hai-Bo Zhang ◽  
Yan Li ◽  
Jian-Lei Wu ◽  
Jian Zhao ◽  
Yun-Jie Tian ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Mrna Expression ◽  
Biological Significance ◽  
Northern China ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Ovarian Endometriosis ◽  
Primary Infertility ◽  
Normal Women

<b><i>Background:</i></b> The aim of the study was to investigate the role of the genetic variation of glutathione S-transferase M1 (<i>GSTM1</i>) in the development of ovarian endometriosis and endometriosis-related primary infertility risk. <b><i>Methods:</i></b> This case-control study included 564 women with ovarian endometriosis and 576 normal women in the control group in northern China. The polymorphism of GSTM1 was genotyped by polymerase chain reaction (PCR)/ligase detection reaction method. To assess the biological significance of polymorphisms, the level of GSTM1 mRNA expression in patients’ endometrial tissues with different genotypes was detected by quantitative real-time PCR (qRT-PCR). <b><i>Results:</i></b> Compared with the positive genotype, the null genotype of <i>GSTM1</i> was associated with the risk of developing ovarian endometriosis (OR = 1.29, 95% CI = 1.02–1.62). Further analysis showed that patients with a null genotype also had a significantly higher risk of primary infertility than patients with positive genotypes (OR = 1.59, 95% CI = 1.01–2.49). In addition, we found that <i>GSTM1</i> mRNA expression was present in the endometrial tissue of all patients, but the expression level of patients with a positive genotype was nearly 10 times higher than that of patients with a negative genotype. <b><i>Conclusion:</i></b> Our results suggest that the <i>GSTM1</i> polymorphism is not only related to the genetic susceptibility to ovarian endometriosis but also a potential molecular marker of primary infertility in patients with ovarian endometriosis.

scholarly journals S-Methyl Cysteine Protective Effects in Oreochromis Niloticus Fish Contaminated by Thiobencarb Herbicide

2020 ◽  
Author(s):  
Mostafa Ali Elmadawy ◽  
Walied Abdo ◽  
Amira Alaa El-Dein Omar ◽  
Nadia B. Mahfouz
Keyword(s):  
Adverse Effects ◽  
Histopathological Examination ◽  
Genotoxic Effect ◽  
Control Group ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Commercial Feed ◽  
Significant Difference ◽  
Treated Fish

Thiobencarb which is a carbamate herbicide is used for managing undesirable weeds during rice cultivation in Egypt. This study was designed to investigate the adverse effects of a field dose of thiobencarb on Nile tilapia and ameliorating the role of the low dose of S-methyl cysteine (SMC). Experimental fishes were divided into four groups; first group was reared without any treatments and served as a control group; the second group was exposed to thiobencarb (36µg/L); the third group was fed on a commercial feed containing 200 mg of SMC/Kg in conjunction with thiobencarb added to aquarium (36µg/L) while, the fourth group was fed on a feed containing 200 mg of SMC/Kg only. Fishes were sacrificed at the end of the experimental course (two months) and sampling was carried out. Catalase, Glutathione S Transferase activities, Glutathione reduced, and Malondialdhyde levels were assayed. Genotoxic effect of thiobencarb and SMC on treated fish was investigated in erythrocytes, gills, and liver tissues using micronucleus and comet assay. Histopathological examination of livers, gills, and brain was also carried out. The results indicated that fish exposed to thiobencarb indicated herbicide dependent oxidative stress and genotoxic effect justified by a significant difference in antioxidant biomarkers as well as nuclear abnormalities and comet parameters compared to control values. Moreover, histopathological findings were in line with other results. SMC ameliorated the adverse effects which were effective in the improvement of DNA and oxidative damage in thiobencarb intoxicated fish.

scholarly journals Comparative toxicity of abamectin and nano-derived form on land snail, Helix aspersa in attributing to cytotoxicity and biochemical alterations

2021 ◽  
Vol 10 (1) ◽  
pp. 296-311
Author(s):  
Khaled Yassin Abdel-Halim ◽  
Safaa Ramadan Osman ◽  
Heba Mohamed El-Danasoury ◽  
Gihan Fathy Aly
Keyword(s):  
Digestive Gland ◽  
Land Snail ◽  
Helix Aspersa ◽  
Land Snails ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Biochemical Alterations ◽  
Nano Emulsion ◽  
Snail Helix Aspersa

The comparative toxic effect of Vertimec® 1.8% EC, Fast Max Super® 8.4% SC and nano-derived form of abamectin (ABM) (1% nano-emulsion) as a dermal contact for 48 h against land snail, Helix aspersa was evaluated at laboratorial trail. Acute toxicity values (LD50) were 6.45, 11.97, and 45.95 µg snail-1 for nano-derived form of ABM, Fast Max Super® and Vertimec®, respectively. Nano-derived form exhibited the highest toxic effects (1.86 and 7.12-folds), respect to Fast Max Super® and Vertimec®. Sublethal doses: 1/10 and 1/100 LD50s of the examined compounds were applied to evaluate some biochemical alterations e.g. acetylcholinesterase (AChE), malondialdhyde (MDA), lactate dehydrogenase (LDH), glutathione-S-transferase (GST), acid phosphatase (ACP), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), respectively, in haemolymph and digestive glands homogenates. The all treatments significantly decreased AChE activity in ganglia homogenate, respect to control group (untreated). All treatments exhibited MDA level and LDH activity greater than the control in both haemolymph and digestive gland. This concept recognizes the cytotoxic effect of ABM on gastropods. Significant declines in GST, ACP, and ALP activities were exhibited in homogenate of digestive gland for the all treatments. However, AST/ALT activities exhibited increase greater than untreated group. These findings may explain the role of these doses of ABM for dysfunction in organs of H. aspersa. Thus, prepared nano-emulsion was more potent toxic on land snails. However, H. aspersa is considered a useful tool to assess ecotoxicological impact of pesticides.

Genetic variation of the E-cadherin gene is associated with primary infertility in patients with ovarian endometriosis

2014 ◽  
Vol 102 (4) ◽  
pp. 1149-1154.e1 ◽  
Author(s):  
Shan Kang ◽  
Yan Li ◽  
Bin Li ◽  
Na Wang ◽  
Rong-Miao Zhou ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Ovarian Endometriosis ◽  
Primary Infertility ◽  
E Cadherin

scholarly journals Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats

2018 ◽  
Vol 46 (3) ◽  
pp. 1073-1085 ◽  
Author(s):  
Yi Qiu ◽  
Ying Wang ◽  
Xiaodong Wang ◽  
Caixia Wang ◽  
Zhong-yuan Xia
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Cognitive Dysfunction ◽  
Signalling Pathway ◽  
Morris Water Maze Test ◽  
Oxygen Mixture ◽  
Control Group ◽  
Aged Rats ◽  
Creb Protein

Objective This study aimed to evaluate the role of the hippocampal 5-hydroxytryptamine-1A (5-HT1A)-mediated cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signalling pathway in sevoflurane-induced cognitive dysfunction in aged rats. Methods Sixty 18-month-old Sprague–Dawley rats were divided into the control (n = 30) and experimental (Sev, n = 30) groups. The experimental group inhaled 50% air/oxygen mixture (2 L/min) and 2% sevoflurane for 4 hours. The control group inhaled 50% air/oxygen mixture (2 L/min) for 4 hours. The Morris water maze test was performed The mRNA expression of 5-HT1A receptor, and cAMP PKA, cAMP response element-binding protein (CREB), and phosphorylated CREB (p-CREB) protein expression were determined. Results The escape latency and swimming distance were greater, and the number of crossings of the platform location and time spent in the platform quadrant were less in the Sev group compared with the control group. cAMP, PKA, CREB, and p-CREB protein expression was downregulated in the Sev group 1 day after anaesthesia compared with the control group. Hippocampal 5-HT1A receptor mRNA expression was higher 7 days after anaesthesia compared with the control group. Conclusion Sevoflurane-induced cognitive dysfunction in aged rats may be related to inhibited expression of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway.

Impact of Factor V Leiden Polymorphism in Patients with PCOS

2019 ◽  
Vol 12 (2) ◽  
pp. 124-130
Author(s):  
Veselin P. Penkov ◽  
Katya S. Kovacheva ◽  
Georgi M. Golemanov ◽  
Galia A. Georgieva ◽  
Peter D. Ivanov ◽  
...  
Keyword(s):  
Polycystic Ovary ◽  
Factor V Leiden ◽  
Control Group ◽  
Factor V ◽  
Healthy Controls ◽  
Single Nucleotide ◽  
Primary Infertility ◽  
History Of ◽  
The Impact

Summary The present study aimed to evaluate the impact of factor V Leiden (FVL) polymorphism within the reproductive problems encountered by patients with polycystic ovary syndrome (PCOS). A total of 92 female patients with PCOS and 101 healthy controls were included in the study. Clinical and laboratory parameters were examined. The full history of each patient was taken. Single nucleotide polymorphism rs6025 in F5 was genotyped in PCOS patients and compared to the genotype frequency of the healthy controls. The data were analysed for correlation with infertility and pregnancy loss in PCOS patients. The prevalence of FVL polymorphism was higher, however not significantly, in PCOS patients compared to that of the control group (respectively OR=2.238, 95 % CI 0.777±6.449, p=0.104). The carriers of FVL polymorphism showed a higher rate of primary infertility (30.0% versus 12.5%, OR=3.143, 9 % CI 0.686±14.388, p=0.047) and their total reproductive failure rate was higher (60.5% versus 47.2%, OR=1.819, 95% CI 0.632±9.259, p=0.117). Carriage of FVL polymorphism in PCOS patients is associated with primary infertility and a presumed cause of the further investigations needed to understand the impact of FVL on PCOS. Carriage of FVL polymorphism in PCOS patients is associated with a higher rate of primary infertility, which draws attention to the role of this factor in the aetiology of the PCOS-related subfertility. Further investigations are needed to understand the impact of FVL on PCOS.

scholarly journals Alpha-Synuclein RNA Expression is Increased in Major Depression

2019 ◽  
Vol 20 (8) ◽  
pp. 2029 ◽  
Author(s):  
Andrea Rotter ◽  
Bernd Lenz ◽  
Ruben Pitsch ◽  
Tanja Richter-Schmidinger ◽  
Johannes Kornhuber ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Lewy Bodies ◽  
Alpha Synuclein ◽  
Control Group ◽  
Expression Levels ◽  
Healthy Control ◽  
Severity Of Depression ◽  
High Comorbidity ◽  
Mrna Expression Levels

Alpha-synuclein (SNCA) is a small membrane protein that plays an important role in neuro-psychiatric diseases. It is best known for its abnormal subcellular aggregation in Lewy bodies that serves as a hallmark of Parkinson’s disease (PD). Due to the high comorbidity of PD with depression, we investigated the role of SNCA in patients suffering from major depressive disorder (MDD). SNCA mRNA expression levels were analyzed in peripheral blood cells of MDD patients and a healthy control group. SNCA mRNA expression was positively correlated with severity of depression as indicated by psychometric assessment. We found a significant increase in SNCA mRNA expression levels in severely depressed patients compared with controls. Thus, SNCA analysis could be a helpful target in the search for biomarkers of MDD.

scholarly journals Role of P 2 receptors in Cerebral Vasospasm

Stroke ◽  
2001 ◽  
Vol 32 (suppl_1) ◽  
pp. 354-355
Author(s):  
John H Zhang ◽  
Hitoshi Kimura ◽  
Robin Carpenter ◽  
Andrew D Parent
Keyword(s):  
Receptor Antagonist ◽  
Mrna Expression ◽  
Cerebral Vasospasm ◽  
Cerebral Arteries ◽  
Control Group ◽  
Arterial Blood ◽  
Transmission Electron ◽  
Intracellular Ca ◽  
Basilar Arteries

P87 Introduction: Extracellular ATP activates P 2 receptors to increase intracellular Ca 2+ , to contract cerebral arteries, and to induce vasospasm in animals. This study examined the mRNA expression of P 2 receptors in a rat double hemorrhage model and explored the therapeutic role of P 2 receptor antagonists in a dog double hemorrhage model. Methods: One hundred SD rats were divided into five groups to undergo double hemorrhage by injecting autologous arterial blood into cisterna magna on day 0 and 2. Rats were sacrificed on day 3, 5 or 7. In sham group, rats were injected with saline. In control group, no surgery was conducted. Basilar arteries were harvested for mRNA isolation and RT-PCR or were fixed for transmission electron microscopy (TEM). Eighteen dogs were divided into three groups to have double hemorrhage and sacrificed on day 7. Two groups were treated, intracisternally, with P 2 receptor antagonist suramin (selective for P 2Y and P 2X ) or PPADS (selective for P 2X1 ) (30 μM) from day 3–6. Angiograph was performed before blood injection and before sacrifice. The basilar arteries were collected for TEM. Results: Mild to moderate vasospasm (30–40% reduction) was observed on day 3–7 in rats. The mRNA expression of P 2X1 receptor was down-regulated (P<0.05) on day 3 and recovered on day 5 and 7. P 2Y1 and P 2Y2 receptors were up-regulated (P<0.05) on day 5 and remain elevated on day 7. No changes were observed in the shame group. Severe vasospasm was observed on day 7 in dogs. P 2 receptor antagonist Suramin but not PPADS significantly reversed (P<0.01, ANOVA) vasospasm in dogs. Discussion: P 2Y1 and P 2Y2 , but not P 2X1 , may be involved in cerebral vasospasm. Up-regulation of P 2Y receptor may enable ATP to produce contraction at low concentrations. Suramin reduced vasospasm probably by blocking P 2Y receptors.

Vinpocetine attenuates fluoxetine-induced liver damage in rats; Role of Nrf2 and PPAR-γ

2021 ◽  
pp. 096032712110515
Author(s):  
Gellan Alaa Mohamed Kamel
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Mrna Expression ◽  
Liver Damage ◽  
B Cell Lymphoma ◽  
Control Group ◽  
Related Factor ◽  
Protective Effects ◽  
Ppar Γ

Background Fluoxetine (FLX) has been widely used as first-line treatment in cases of depression and other neuropsychiatric disorders. Although its safety has been approved, the use of FLX was associated with liver injury and chronic liver disease. Vinpocetine (Vinpo), a nootropic drug, possesses antioxidant and anti-inflammatory effects. Objective This study aimed to evaluate the protective effects of Vinpo on FLX-induced liver damage pointing to the role of peroxisome proliferator-activated receptor-gamma (PPAR-γ) and nuclear factor erythroid 2-related factor 2 (Nrf2). Methods Rats were randomized to four groups: control group, Vinpo group (20 mg/kg/day; orally), FLX group (10 mg/kg/day; orally), and Vinpo + FLX group. Results FLX-induced liver damage was evidenced through elevated liver function biomarkers and induced hepatic histopathological changes. Concurrent Vinpo treatment resulted in a significant decrease in hepatotoxicity biomarkers and histopathological alterations. FLX-induced oxidative stress and inflammation were attenuated by Vinpo. In addition, Vinpo attenuated the hepatic NRF2 and HO-1 levels and up-regulated PPAR-γ expression. Moreover, FLX elevated Bcl-2-associated X protein (Bax) mRNA expression and decreased B-cell lymphoma 2 (Bcl2) mRNA expression were markedly reversed by Vinpo. Conclusion Vinpo possesses ameliorative effects against FLX-induced liver injury in rats. This effect may be due to attenuation of oxidative stress and inflammation, in addition to upregulation of PPAR-γ expression.

Silent Information Regulator 1 (SIRT1) Promotes Pancreatic β-Cell Mitochondrial Autophagy and Pyrin Domains-Containing Protein 3 (NLRP3) Activation Under High Glucose Environment

2022 ◽  
Vol 12 (3) ◽  
pp. 647-652
Author(s):  
Yang Zhang ◽  
Peng Sun ◽  
Shuying Han ◽  
Duojiao Fan
Keyword(s):  
Inflammatory Response ◽  
Mrna Expression ◽  
High Glucose ◽  
Control Group ◽  
Β Cell ◽  
Blank Group ◽  
Qrt Pcr ◽  
Silent Information Regulator 1 ◽  
Inhibition Group

Mitochondrial autophagy and inflammatory response involves in diabetes. This study mainly explores the role of Silent Information Regulator (SIRT1) in pancreatic β-cells under high glucose conditions and related mechanism. Pancreatic β cells was cultured in a high-glucose environment with SRT1720 and EX527 respectively to define activation group and inhibition group followed by analysis of SIRT1, P-FOXO1, FOXO1, LC3, ATG5, PINK, Parkin, Mfn1, Mfn2, Fis1, IL-6, TNF-α, NLRP3 protein and mRNA expression by qRT-PCR, Western blot and fluorescent probe technology. Compared with control group, SIRT1 protein and mRNA expression in the high glucose group was significantly reduced. Activation group had highest protein and mRNA expression of SIRT1 P-FOXO1, FOXO1, Mfn1, Mfn2, Fis1, PINK, Parkin and mitochondrial membrane potential followed by blank group and inhibition group.SIRT1 secretion by pancreatic β-cells under high glucose environment is reduced. After activating SIRT1, mitochondrial autophagy decreased significantly and inflammatory response is significantly alleviated, indicating that SIRT1 might be used as a therapeutic target.

Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

2006 ◽  
Vol 76 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yukari Egashira ◽  
Shin Nagaki ◽  
Hiroo Sanada
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Enzyme Activities ◽  
Treated Group ◽  
Control Group ◽  
Puromycin Aminonucleoside ◽  
Low Level ◽  
Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

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