scholarly journals Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery

2021 ◽  
Author(s):  
Binbin Zhu ◽  
Yahui Liu ◽  
Datun Qi ◽  
Linwei Zhao ◽  
Xiaohang Yang ◽  
...  
Keyword(s):  
Renal Artery ◽  
High Fat Diet ◽  
Renal Fibrosis ◽  
Kidney Tissue ◽  
Blood Lipid ◽  
Control Group ◽  
Model Group ◽  
High Fat ◽  
Tgf Β1 ◽  
Blood Lipid Levels

Abstract Background This study aims to compare whether two different routes of Renal denervation (RDN) from the intima and adventitia of the renal artery can reduce renal fibrosis in a pig model of hypertension induced by a high-fat diet and to explore possible molecular mechanisms. Methods Twenty-four Bama miniature pigs were randomly divided into a control group (normal diet, n=6) or a hypertension model group (high-fat diet, n=18). The model group was randomly divided into the intima-RDN group (n=6), the adventitia-RDN group (n=6), or the renal arteriography only group (sham group, n=6). All animals were fed separately. The model group was fed a high-fat diet after the operation, and the control group was fed conventionally for 6 months. After 6 months, renal artery angiography was performed again to observe the condition of the renal arteries, after which all animals were euthanized. The blood pressure (BP) and blood biochemical results of each group were evaluated 6 months after the operation; kidney tissue morphology and collagen fiber content were examined by hematoxylin-eosin (HE) staining and Masson staining; Superoxide dismutase (SOD) activity and the malondialdehyde (MDA) content of kidney tissue were assessed by a biochemical enzyme method; the protein expression level of transforming growth factor-β 1 (TGF-β1), α smooth muscle actin (αSMA) and Smad3 were assessed by Western blot; and electron microscopy was used to examine changes in kidney microstructure. Results After 6 months of a high-fat diet, the blood lipid levels of the model group were significantly higher compared to baseline and to that of the control group during the same period (all showed P<0.05); the blood lipid levels of the control group did not change significantly from baseline (P>0.05). The degree of glomerular damage caused by hyperlipidemia in the intima-RDN group and the adventitia-RDN group was significantly lower than that of the sham and control groups, and the renal fibrosis area percentage was also significantly lower (P<0.05). Electron microscopy showed that both the intima-RDN group and the adventitia-RDN group had a more even distribution of chromosomes and less mitochondrial swelling compared with the sham group. Conclusion RDN from the adventitia of the renal artery and RDN from the intima of the renal artery have the similar advantages of delaying high fat-induced renal fibrosis. The anti-fibrotic effect of RDN may be related to inhibition of the TGF-β1/smad3 pathway.

Effect of Armillaria mellea on Blood Lipid Levels and Antioxidant Enzymes Activity in High Fat Mice

2014 ◽  
Vol 884-885 ◽  
pp. 423-428
Author(s):  
Yan Hui Yang ◽  
Guo Qiang Zheng ◽  
Juan Tang ◽  
Yue Meng Wang ◽  
Chuan Wang Zhu ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
High Fat Diet ◽  
Blood Lipid ◽  
Lipid Levels ◽  
Model Group ◽  
High Fat ◽  
Treatment Groups ◽  
Armillaria Mellea ◽  
And Oxidative Stress ◽  
Blood Lipid Levels

The effect of Armillaria mellea on blood lipid levels and oxidative stress in mice fed on high-fat diet was investigated. Animals were allocated to the Armillaria mellea polysaccharides-treatment groups (I, II) and Armillaria mellea oligosaccharides-treatment groups (I, II). All mice were fed with high-fat diet for 40 days but control mice with basic diet. TC, TG, HDL-c, LDL-c were measured by enzymatic and colorimetric methods. The same, MDA,SOD, GSH-PX were measured. Results showed that administration of Armillaria mellea polysaccharides and oligosaccharides significantly increased antioxidant enzymes GSH-Px activities and decreased TC, TG, LDL-c, MDA level in mice (P < 0.05) compared with model group. In conclusion Armillaria mellea polysaccharides and oligosaccharides were able to protect mices antioxidative and improve abnormal blood lipid levels.

scholarly journals Chronic stress: a crucial promoter of cell apoptosis in atherosclerosis

2019 ◽  
Vol 48 (1) ◽  
pp. 030006051881460 ◽  
Author(s):  
Ling-bing Meng ◽  
Meng-jie Shan ◽  
Ze-mou Yu ◽  
Jian Lv ◽  
Ruo-mei Qi ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Cell Apoptosis ◽  
High Fat Diet ◽  
Control Group ◽  
Model Group ◽  
Balloon Injury ◽  
High Fat ◽  
Caspase 9 ◽  
Stress Group ◽  
Diet Model

Objective Chronic stress may lead to augmented incidence rates of coronary and cerebrovascular diseases associated with atherosclerosis. However, few studies have focused on the effect of chronic stress on atherosclerosis plaque formation. Therefore, this study was designed to directly evaluate how chronic stress affects atherosclerosis. Methods Thirty rabbits were divided into three groups: the control group, balloon-injury operation + high-fat diet model group, and chronic stress + balloon-injury operation + high-fat diet model group. Physical and social stress were induced, and proteomic methods were applied to identify specific markers. Results After protein determination, the chronic stress + balloon-injury operation + high-fat diet model group exhibited significant upregulation of the following apoptosis-related proteins: UBE2K, caspase 3, caspase 9, BAX, P53, and FAS. In particular, real-time polymerase chain reaction showed that the protein expression of caspase 9 was significantly downregulated in the stress group compared with the non-stress groups. However, the other proteins showed significantly increased expression in the stress group. Conclusion Chronic stress may promote cell apoptosis in the physiopathologic process of atherosclerosis.

Vitamin K2 can suppress the expression of Toll-like receptor 2 (TLR2) and TLR4, and inhibit calcification of aortic intima in ApoE-/- mice as well as smooth muscle cells

Vascular ◽  
2017 ◽  
Vol 26 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Zhaojun Wang ◽  
Zhongqun Wang ◽  
Jie Zhu ◽  
Xinguang Long ◽  
Jinchuan Yan
Keyword(s):  
Vascular Calcification ◽  
High Fat Diet ◽  
Treated Group ◽  
Vitamin K2 ◽  
Normal Diet ◽  
Control Group ◽  
Model Group ◽  
High Fat ◽  
Expression Levels ◽  
Tlr4 Mrna

Background and objectives Vascular calcification is a common complication in atherosclerosis. Accumulating evidence showed that Toll-like receptors (TLRs) mediate pro-inflammatory and atherosclerosis. Recent studies demonstrated that vascular calcification is one of the detrimental effects of vitamin K (Vit K) antagonists. However, the effects of Vit K on the expression of TLR2 and 4 and intimal calcification in artery remained unidentified. Methods and results Eighteen ApoE-/- mice were randomly divided into model group, Vit K-treated group, and control group. The mice of model and Vit K-treated group were fed with high-fat diet, while control group mice were fed with normal diet. Mice of Vit K-treated group were administered orally with vitamin K2 (40 mg.kg−1.day−1) for 12 weeks. Twelve weeks later the aortic sections of mice were acquired and stained with hematoxylin and eosin and von Kossa, respectively. Calcium content and activity of alkaline phosphatase (ALP) at aortic tissues were measured. The expression levels of TLR2 and TLR4 in aorta sections were detected by immunohistochemisty and RT-PCR, respectively. The effects of Vit K on cellular calcification were further studied in A7r5 SMCs. Results demonstrated that high-fat diet induced typical atherosclerosis with intimal calcification in ApoE-/- mice, while in Vit K-treated group atherosclerosis and calcium deposits were not serious; Vit K2 also inhibited cellular calcification in A7r5 SMCs. Quantitative analysis showed that calcium and ALP activity at aortic tissues in the Vit K-treated mice were significantly lower than that of the model group ( P < 0.01); Compared to the control group, the expression levels of TLR2 and TLR4 in the model group were significantly higher ( P < 0.05), while in Vit K-treated group the levels of TLR2 and 4 were significantly lower than that in the model group. Furthermore, the content of calcium was positively related to the expression levels of TLR2 and TLR4 mRNA at aortic tissues ( r = 0.77 and r = 0.79, respectively, both P < 0.001). Conclusion VitK2 can inhibit intimal calcification of aortic artery induced by high-fat diet in ApoE-/- mice and A7r5 SMCs calcification induced by β-sodium glycerophosphate, and meanwhile can reduce the expression of TLR2 and TLR4. These results suggested that the effects of VitK2 on vascular calcification may be associated with the expression of TLR2 and TLR4.

scholarly journals Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice

2020 ◽  
Vol 17 (15) ◽  
pp. 5513
Author(s):  
Supattra Prom-in ◽  
Jasadee Kaewsrichan ◽  
Nuntika Wangpradit ◽  
Chua Kien Hui ◽  
Mohamad Fairuz Yahaya ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Normal Diet ◽  
Learning Ability ◽  
Control Group ◽  
Therapeutic Effects ◽  
Memory Retention ◽  
High Fat ◽  
Group I

Okra peel exhibits numerous therapeutic effects. This study explores the potential ameliorative effects of okra peel powder on high-fat-diet (HFD)-induced hypercholesterolemia and cognitive deficits. Thirty-six C57BL/6J male mice were randomly divided into six groups (n = 6 per group): (i) control, mice fed with a normal diet; (ii) HFD, mice fed with HFD; (iii) HFD-SIM, mice fed with HFD and given simvastatin (20 mg/kg/day); (iv) HFD-OP1; (v) HFD-OP2; (vi) HFD-OP3, mice fed with HFD and okra peel (200, 400, or 800 mg/kg/day, respectively). Following 10 weeks of treatments, the mice were subjected to the Morris water maze (MWM). Parameters such as weekly average body weight, food intake, and blood lipid profiles were also recorded. The HFD group showed a profound increase in total cholesterol and low-density lipoprotein concentration compared to the control group. All okra-treated and HFD-SIM groups performed better than the HFD group during acquisition trials, whereas only the HFD-OP1 produced a significantly higher number of entries into the platform zone during the probe trial. In sum, all three okra doses improved the learning ability of the mice. However, only the lowest dose of okra significantly improved the spatial reference memory retention.

Hepatic PTP1B Expression Involvement in the Effects of Chinese Medicine Formula Xiao-Gao-Jiang-Zhuo Using an Obese Rat Model

2011 ◽  
Vol 39 (02) ◽  
pp. 301-313 ◽  
Author(s):  
Min Li ◽  
Bai Chang ◽  
Zhong Zhen ◽  
Pei-Jie Qin ◽  
Wen-Ke Liu ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Serum Insulin ◽  
Blood Lipid ◽  
Abdominal Fat ◽  
The Body ◽  
High Dose ◽  
Model Group ◽  
High Fat ◽  
Obese Rats

In this study, we investigated the effects of a Chinese herbal medicine formula Xiao-Gao-Jiang-Zhuo (XGJZ) in obese rats induced by a high-fat diet. Ten male rats in the normal group were fed with a standard diet. Another 50 male obese rats were induced by a 12-week high-fat diet feeding, and were randomly divided into five groups (n = 10 per group): the model group, the high-dose XGJZ group, the middle-dose XGJZ group, the low-dose XGJZ group, and the sibutramine group. After 14 weeks of treatment, body weight, abdominal fat, blood lipid and serum insulin level were measured, and the protein and gene expression of PTP1B in liver tissue was tested. Our data showed that the body weight of the high-dose and middle-dose groups and the sibutramine group had significant differences in comparison with the model group (p < 0.05), and all three dose groups had significantly reduced abdominal fat (p < 0.05). The triglyceride level of the three dose groups and the sibutramine group, and the total cholesterol level of the middle-dose group were all significantly reduced (p < 0.05). The serum insulin of the high-dose and middle-dose groups also decreased significantly (p < 0.05). The expression of hepatic PTP1B mRNA of the three dose groups decreased significantly in comparison with the model group (p < 0.05 or 0.01). The expression of hepatic PTP1B protein of the high-dose and middle-dose groups decreased significantly (p < 0.05). Our data suggested that XGJZ can modulate the body weight, abdominal fat and blood lipid in the obese rats, and this modulation might improve insulin resistance by inhibiting the PTP1B signal pathway.

scholarly journals The Correlation between MicroRNA-199a and White Adipose Tissue in C57/BL6J Mice with High-Fat Diet

2017 ◽  
Author(s):  
Dan Liu ◽  
Xia Wang ◽  
Xinying Lin ◽  
Baihui Zhang ◽  
Shue Wang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Therapeutic Potential ◽  
Binding Protein ◽  
Regulatory Element ◽  
Control Group ◽  
Model Group ◽  
High Fat ◽  
Fatty Acid Binding

AbstractUnderstanding is emerging about microRNAs as mediators in the regulation of white adipose tissue (WAT) and obesity. The expression level of miR-199a in mice was investigated to test our hypothesis: miR-199a might be related to fat accumulation and try to find its target mRNA, which perhaps could propose strategies with a therapeutic potential affecting the fat storage. C57/BL6J mice were randomly assigned to either a control group or an obesity model group (n=10 in both groups). Control mice were fed a normal diet (fat: 10 kcal %) ad libitum for 12 weeks, and model mice were fed a high-fat diet (fat: 30 kcal %) ad libitum for 12 weeks to induce obesity. At the end of the experiment, body fat mass and the free fatty acids (FFAs) and triglycerides (TGs) in WAT were tested. Fat cell size was measured by hematoxylin-eosin (H&E) staining method. The fat mass of the model group was higher than that of the control group (P<0.05). In addition, the concentrations of the FFAs and TGs were higher (P<0.05) and the adipocyte count was lower (P<0.05) in the model group. We tested the expression levels of miR-199a and key adipogenic transcription factors, including peroxisome proliferator activated receptor gamma2 (PPARγ2), CCAAT/enhancer binding proteins alpha (C/EBPα), adipocyte fatty acid-binding protein (aP2), and sterol regulatory element binding protein-1c (SREBP-1c). Up-regulated expression of miR-199a was observed in model group. Increased levels of miR-199a was accompanied by high expression levels of SREBP-1c. We found that the 3’-UTR of SREBP-1c mRNA has a predicted binding site for miR-199a. Based on the current discoveries, we suggest that miR-199a may exert its action by binding to its target mRNA and cooperate with SREBP-1c to induce obesity. Therefore, if the predicted binding site is confirmed by further research, miR-199a may have therapeutic potential for obesity.AbbreviationsWAT, white adipose tissue; PPARγ2, peroxisome proliferator, activated receptor γ2; C/EBP αCCAAT/enhancer binding proteins α; aP2, adipocyte fatty acid-binding protein; SREBP-1c, sterol regulatory element binding protein-1c; HFD, high-fat diet.

scholarly journals Ruanmailing Oral Liquid Inhibit Atherosclerosis in ApoE-/- Mice by Regulation of TGF-β1/SMAD4 Signaling Pathway

2021 ◽  
Author(s):  
Tianmin Wu ◽  
Xuanbin Huang ◽  
Jinshui Chen ◽  
Wenjuan Xue ◽  
Liufang Fan ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Thoracic Aorta ◽  
High Fat Diet ◽  
Pathological Examination ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Dose ◽  
Model Group ◽  
High Fat ◽  
Oral Liquid ◽  
Tgf Β1

Abstract BackgroundTo investigate the effect of Ruanmailing oral liquid on atherosclerosis and TGF-β1/SMAD4 signaling pathway in ApoE knockout mice induced by high-fat diet. MethodsForty ApoE-/- mice were randomly divided into 5 groups, and mice fed with standard diets were the control group. ApoE-/- mice high-fat diet induced atherosclerotic phenotype. After grouping and treatment, they were divided into high-fat feeding model group, low-dose and high-dose of Ruanmailing groups (1.75, 4.55 ml/kg/d), Lipitor Group (3.0 mg/kg/d). After 12 weeks of administration, blood was collected from the mice orbit to determine the levels of TC, TG, LDL-C, and HDL-C, and the pathological changes of thoracic aorta atherosclerosis were observed. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of serum TGF-β1, and RT-PCR and Western Blot were used to detect the expression of SMAD4 and GATA2 in the thoracic aorta of ApoE-/- mice in each group. ResultsCompared with the high-fat model group, the serum lipids level of each administration group were reduced (P<0.01 or P<0.05), and the ratio of plaque area to luminal area (W/L) was significantly reduced (P<0.05), and pathological examination indicated atherosclerotic lesions in thoracic aorta of ApoE-/- mice were alleviated, and the high-dose Ruanmailing group had the most significant anti-atherosclerotic effect. ConclusionsRuanmailing oral liquid has an anti-atherosclerotic effect, and its mechanism may be related to the intervention of GATA2 in the TGF-β1/SMAD4 signaling pathway to reduce the differentiation and proliferation of arterial smooth muscle cells.

scholarly journals Corn silk polysaccharide ameliorates high fat diet induced hepatic steatosis in mice

2021 ◽  
Vol 271 ◽  
pp. 03027
Author(s):  
Wang Tailin ◽  
Wang Zhiwen ◽  
Liu Yi ◽  
Huang Li
Keyword(s):  
Food Intake ◽  
Normal Control ◽  
High Fat Diet ◽  
Statistical Significance ◽  
Normal Control Group ◽  
Control Group ◽  
Model Group ◽  
High Fat ◽  
Corn Silk ◽  
Intake Water

To study the therapeutic effect of corn silk polysaccharide (CSP) on NAFLD mice induced by high fat diet. C57BL/6J mice were divided into normal control group (NC), high fat diet (HFD) group, HFD+200 mg/kg CSP group, and HFD+600 mg/kg CSP group. NAFLD mouse model was established by HFD feeding. Blood and liver tissues of each group were collected and biochemical and pathological tests were performed. The energy intake of NAFLD model group was higher than that of normal control group, and the food intake, water intake, and excretion of NAFLD model group were lower than that of normal control group. There was no statistical significance in the food intake, energy intake, water intake, and excretion of CSP group compared with that of NAFLD model group, nor was there any statistical significance between CSP and two doses of CSP. Biochemical tests showed that CSP decreased the levels of alanine aminotransferase, aspartate aminotransferase, triglyceride and total cholesterol in serum of HFDfed mice, and inhibited the expressions of IL-6 and TNF-α in liver tissue. Pathological results showed that CSP improved HFD-induced hepatic steatosis.

scholarly journals A potential probiotic- Lachnospiraceae NK4A136 group: Evidence from the restoration of the dietary pattern from a high-fat diet

2020 ◽  
Author(s):  
Menq-Rong Wu ◽  
Te-Sen Chou ◽  
Ching-Ying Huang ◽  
Jong-Kai Hsiao
Keyword(s):  
Dietary Pattern ◽  
High Fat Diet ◽  
Blood Lipid ◽  
Hepatic Function ◽  
Physiological Parameters ◽  
Control Group ◽  
Chow Diet ◽  
High Fat ◽  
Short Term ◽  
Dietary Shift

Abstract Background: High-fat diet (HFD) that contributes to obesity is one of the pivotal risk factors for metabolic syndrome and cancers. The dietary pattern can shape the intestinal bacterial community and influence the physiological parameters. This study aimed to investigate whether the short-term dietary pattern shift from HFD to a balanced chow diet (CD) could correct HFD-induced colonic dysbiosis and reverse adverse health effects and identify the specific bacteria that changed by dietary patterns. Results: C57BL/6 mice fed with an HFD for 10 months, followed by a CD for 3 months, served as the dietary shift model. Stool samples were collected for bacterial analysis. Physiological parameters, such as serum adipokines, blood lipid levels, and hepatic function, were monitored in control and dietary shift groups. HFD-induced weight gain was mitigated by the dietary shift. A highly similar structure at the phylum, genus, and species levels was observed in the beta diversity of colonic bacteria in mice that underwent the dietary shift as compared to those in the control group. Notably, the abundance of Peptococcaceae and Akkermansiaceae in HFD-fed mice reduced after the dietary shift; whereas the diminished amount of probiotic Lachnospiraceae NK4A136 group in HFD-fed mice was restored to the level comparable to those in controls after the dietary shift. Conclusions: Our finding suggests that a dietary switch from a long-term HFD to a short-term balanced diet has the potential to correct colonic dysbiosis and restore physiological homeostasis. The Lachnospiraceae NK4A136 group has the potential to be a probiotic.

scholarly journals Ameliorating effects of Sporidiobolus pararoseus extract on dyslipidemia in mice with high fat diet induced obesity

2018 ◽  
Vol 96 (5) ◽  
pp. 695-701 ◽  
Author(s):  
Chao Du ◽  
Danyu Ying ◽  
Yahui Guo ◽  
Yuliang Cheng ◽  
Mei Han ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
High Dose ◽  
Model Group ◽  
High Fat ◽  
Obese People ◽  
Sporidiobolus Pararoseus

The study investigated how an extract of Sporidiobolus pararoseus (S.p.) affects lipid metabolism in Kunming mice that were obese as a result of being fed a high-fat diet; the control group were administered Max EPA fish oil. Ten mice were randomly selected from a pool of 60 mice for the control group and the remaining 50 mice were fed with a high-fat diet to establish a dyslipidemia model. After 4 weeks, these 50 mice were randomly distributed among 5 groups: high-fat model group; Max EPA group; and 3 groups of mice fed different doses of S.p. extract (low dose, medium dose, and high dose). After 8 weeks, the mice were sacrificed and the relevant parameters were measured. Compared with the high-fat model group, the group administered the high dose of S.p. extract showed significantly decreased body mass and serum levels of total cholesterol, triglycerides, and low-density lipoprotein cholesterol, and increased levels of high-density lipoprotein cholesterol. The results from RT-PCR showed that the mRNA expression of sterol regulatory element-binding protein 1c, fatty acid synthesis enzyme, and acetyl-CoA carboxylase was lower in the groups supplemented with S.p. extract than in the high-fat model group, whereas the expression of carnitine palmitoyltransferase 1 was higher in the group supplemented with S.p. extract than in the high-fat model group. Our results suggest that taking S.p. extract could benefit patients with dyslipidemia. Therefore, S.p. extract should be developed as a dietary supplement to improve lipid metabolism in obese people.

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