scholarly journals Особливості цитокінового профілю та імунного статусу у хворих на кондиломи аногенітальної ділянки

2021 ◽  
pp. 45-49
Author(s):  
А. М. Слепічко ◽  
І. М. Дейкало
Keyword(s):  
Ig G

Мета роботи: вивчити зміни клітинного та гуморального імунітету у хворих на гострокінцеві кондиломи аногенітальної ділянки та визначити ступінь порушень; дослідити стан цитокінового профілю у хворих на ГК аногенітальної ділянки як факторів прогнозування перебігу післяопераційного періоду та ефективності лікування. Матеріали і методи. Обстежено 105 хворих на ГК аногенітальної ділянки: 63 (60 %) жінки та 42 (40 %) чоловіки. Вивчено стан клітинного та гуморального імунітету та рівень цитокінів TNFa, IL-4, IL-6, IL-8. Результати досліджень та їх обговорення. Частота хронічних соматичних захворювань склала 96 (91,43 %). Визначено достовірне зниження абсолютної та відносної кількості лімфоцитів та їх Т-популяцій. На тлі Т-лімфопенії визначено підвищення рівня В-лімфоцитів (CD22) в абсолютній та відносній кількості та Ig A та Ig G, що свідчить про наявність хронічного запального процесу та / або вірусного запалення з виснаженням Т-ланки імунітету. Рівень IL-4 був вищим, ніж у контрольній у 2,4 раза, та дорівнював (4,14 ± 0,24) пг/мл; IL-8 – у 1,9 – (29,82 ± 3,15) пг/мл; TNFa – у 3,0 – (7,64 ± 0,93) пг/мл; IL-6 – у 2,7 – (3,77 ± 0,45) пг/мл. Виявлено залежність рівня TNFa, IL-6, IL-4 та IL-8 від розповсюдження гострокінцевих кондилом та присутності супутнього перифокального запалення з найвищими значеннями при кондиломах Бушке–Левенштейна, тоді як у хворих на поодинокі гострокінцеві кондиломи цитокіновий профіль не відрізнявся від контрольної групи. У хворих на гострокінцеві кондиломи аногенітальної ділянки зсуви в ланках клітинного та гуморального імунітету характеризуються виснаженням ланки клітинного імунітету – загальною лімфопенією, Т-лімфопенією зі зниженням їхньої абсолютної та відносної кількості в усіх субпопуляціях, підвищенням рівня В-лімфоцитів, Ig G та Ig A. Підвищення рівня цитокінів IL-4, IL-6, IL-8 та TNFa у хворих на ГК аногенітальної ділянки відображає ступінь розповсюдження гострокінцевих кондилом та відповідає характеру перифокального запального процесу, що доцільно використовувати як фактор прогнозування перебігу післяопераційного періоду та ефективності лікування.

Low-frequency ultrasound and photochromotherapy in the complex treatment of patients with chronic bacterial and viral rhinosinusitis

2020 ◽  
pp. 13-23
Author(s):  
Natalya Kornova ◽  
A. Krylov
Keyword(s):  
Chronic Rhinosinusitis ◽  
Low Frequency ◽  
Chronic Tonsillitis ◽  
Complex Treatment ◽  
Resonance Imaging ◽  
X Ray ◽  
Low Frequency Ultrasound ◽  
Viral Nature ◽  
Ig G ◽  
Frequency Ultrasound

The article presents results of a study on the effectiveness of using lowfrequency ultrasound and photochromotherapy in the complex treatment of patients with chronic bacterial and viral rhinosinusitis. The study involved 69 people aged 18 to 55 years: 39 (56.83 %) women and 30 (45.11 %) men with chronic rhinosinusitis during the period of clinical exacerbation and concomitant chronic tonsillitis without exacerbation. All patients included in the study underwent X-ray examination or computed tomography and magnetic resonance imaging of the paranasal sinuses. 100 % of the patients were examined for the presence of antibodies to Human gammaherpesvirus 4 (ELISA), had study of blood serum for IgM and IgG and test to determine the avidity of Ig G antibodies to γ-herpesviruses. The obtained data indicate the effectiveness of including low-frequency ultrasound and photochromotherapy in the complex treatment of patients with chronic rhinosinusitis and concomitant chronic tonsillitis of bacterial and viral nature without exacerbation.

scholarly journals SARS-CoV-2 Ig G among Healthcare Workers and the General Population

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 465
Author(s):  
Gregorio P. Milani ◽  
Mario G. Bianchetti ◽  
Giuseppe Togni ◽  
Andreas W. Schoenenberger ◽  
Franco Muggli
Keyword(s):  
General Population ◽  
Healthcare Workers ◽  
Geographical Area ◽  
Healthcare Personnel ◽  
Protein Subunit ◽  
Positive Serology ◽  
Seroprevalence Data ◽  
And Gender ◽  
Few Data ◽  
Ig G

It is assumed that healthcare workers are at the highest risk to be infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, few data from healthcare workers who do not primarily take care of patients with SARS-CoV-2 infection support this assumption. We investigated the prevalence of immunoglobulin G (Ig G) against SARS-CoV-2 among healthcare workers who do not primarily take care of patients with SARS-CoV-2 infection and the general population in a well-defined geographical area. The first part of the study was conducted in May 2020 in Val Mesolcina (Southern Switzerland), a valley with ~8000 inhabitants. All healthcare workers were invited. All participants (n = 488) of the Swiss Longitudinal Cohort Study (SWICOS), a cohort representative of the general population, were also invited. Circulating Ig G against spike protein subunit 1 of SARS-CoV-2 were tested in each subject. Subjects with positive Ig G were tested again after 6 months. The condition of being a healthcare worker, rather than a part of the general population, was tested as a predictor of seroprevalence positivity by both simple and multiple (adjusted for age and sex) logistic regression. Eleven (2.6%) of the 423 SWICOS participants and 46 (16%) out of 289 healthcare workers were positive for antibodies against SARS-CoV-2. The seroprevalence OR was 7.01 (95% CI: 3.53–15.47) for healthcare workers as compared to SWICOS participants. After adjusting for age and gender, the seroprevalence OR was 5.13 (95% CI: 2.54–10.40). About three quarters of the subjects in the SWICOS (73%) and in healthcare (79%) group with a previous positive serology still presented positive Ig G against the SARS-CoV-2 after 6 months. The present seroprevalence data point out that the SARS-CoV-2 infection is seven times higher among healthcare workers than in the general population of Val Mesolcina. Efforts to effectively protect all the healthcare personnel are needed.

scholarly journals Treating Critically Ill Patients Experiencing SARS-CoV-2 Severe Infection with Ig-M and Ig-A Enriched Ig-G Infusion

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 930
Author(s):  
Alberto Corona ◽  
Giuseppe Richini ◽  
Sara Simoncini ◽  
Marta Zangrandi ◽  
Monica Biasini ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Standard Therapy ◽  
Severe Infection ◽  
Treated Group ◽  
Simplified Acute Physiology Score ◽  
Retrospective Case ◽  
Baseline Characteristics ◽  
Time Of Admission ◽  
Ig G ◽  
Control Study

SARS-CoV-2 in patients who need intensive care unit (ICU) is associated with a mortality rate ranging from 10 to 40–45%, with an increase in morbidity and mortality in presence of sepsis. We hypothesized that IgM and IgA enriched immunoglobulin G may support the sepsis-related phase improving patient outcome. We conducted a retrospective case–control study on 47 consecutive patients admitted to our ICU. At the time of admission, patients received anticoagulants (heparin sodium) together with the standard supportive treatment. We decided to add IgM and IgA enriched immunoglobulin G to the standard therapy. Patients receiving IgM and IgA enriched immunoglobulin G were compared with patients with similar baseline characteristics and treatment, receiving only standard therapy. The mortality resulted significantly higher in patients treated with standard therapy only (56.5 vs. 37.5%, p < 0.01) and, at day 7, the probability of dying was 3 times higher in this group. Variable life adjustment display (VLAD) was 2.4 and -2.2 (in terms of lives saved in relation with those expected and derived from Simplified Acute Physiology Score II) in the treated and not treated group, respectively. The treatment based on IgM and IgA enriched immunoglobulin G infusion seems to give an advantage on survival in SARS-CoV-2 severe infection.

scholarly journals Plasma Amyloid-β in Relation to Antibodies Against Herpes Simplex Virus, Cytomegalovirus, and Chlamydophila pneumoniae

2021 ◽  
pp. 1-7
Author(s):  
Karin Lopatko Lindman ◽  
Bodil Weidung ◽  
Jan Olsson ◽  
Maria Josefsson ◽  
Anders Johansson ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Antimicrobial Properties ◽  
Amyloid Β ◽  
Chlamydophila Pneumoniae ◽  
Immunosorbent Assays ◽  
Simplex Virus ◽  
Ig G ◽  
Xmap Technology

Background: Amyloid-β (Aβ), the key constituent of Alzheimer’s disease (AD) plaques, has antimicrobial properties. Objective: To investigate the association between plasma Aβ and antibodies against the AD-related pathogens herpes simplex virus (HSV), cytomegalovirus (CMV), and C. pneumoniae. Methods: Plasma from 339 AD cases, obtained on average 9.4 years (±4.00) before diagnosis, and their matched controls were analyzed for Aβ40 and Aβ42 concentrations with Luminex xMAP technology and INNOBIA plasma Aβ-form assays. Enzyme-linked immunosorbent assays were utilized for analyses of anti-HSV immunoglobulin (Ig) G, anti-HSV1 IgG, anti-HSV2 IgG, anti-CMV IgG, and anti-C. pneumoniae IgG. Follow-up samples were available for 163 of the cases. Results: Presence and levels of anti-HSV1 IgG, anti-HSV2 IgG, anti-CMV IgG, and anti-C. pneumoniae IgG did not correlate with concentrations of Aβ42 or Aβ40 in cases or controls. Conclusion: Levels of plasma Aβ were not associated with antibodies against different AD-related Spathogens.

Clinicopathological characteristics of primary immunoglobulin A nephropathy with immunoglobulin M deposition

2021 ◽  
Author(s):  
Han Ouyang ◽  
Jian Wen ◽  
Kai Song ◽  
Huaying Shen
Keyword(s):  
Immunoglobulin A ◽  
Clinicopathological Characteristics ◽  
Immunoglobulin M ◽  
Endothelial Cell Proliferation ◽  
Negative Group ◽  
Immunoglobulin A Nephropathy ◽  
Positive Group ◽  
Renal Tubular ◽  
The Relationship ◽  
Ig G

IntroductionImmunoglobulin (Ig) G deposition in patients with IgA nephro­pathy (IgAN) often indicates poor prognosis, but the relationship between IgM deposition and the clinicopathology of IgAN remains controversial. The purpose of this study is to further understand the relationship between IgM deposition and IgAN, so as to provide a basis for clinical evaluation and treatment.Material and methodsWe included a total of 839 IgAN patients from the nephropathy departments of 2 hospitals; there were 162 IgM-positive patients and 677 IgM-negative patients. Clinical and pathological data were retrospectively analysed. In addition, a multifaceted comparison was made between the IgM-positive group and the IgM-negative group.ResultsThe serum albumin and IgG levels of the IgM-positive group were lower than those of the IgM-negative group, and the levels of low-density lipo­protein, 24 h proteinuria, and IgM were higher than those of the IgM-nega­tive group. The proportion of endothelial cell proliferation (E1), segmental sclerosis or adhesion (S1), and renal tubular interstitial score in the IgM-posi­tive group were all higher than those in the IgM-negative group. Immunofluo­rescence results showed that the proportion of IgM-positive combination and IgG and C1q deposition was higher than that in the IgM-negative group.ConclusionsImmunoglobulin A nephropathy patients with IgM deposition have relatively poor clinical biochemical indicators, and the degree of renal pathological damage is also relatively serious.

PSX-B-22 Humoral factors of protection of the vaginal mucosa in healthy cows and with mycoplasmosis

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 273-273
Author(s):  
Julia V Busharova ◽  
Roman M Vasilev ◽  
Svetlana V Vasileva ◽  
Vyacheslav A Trushkin ◽  
Anastasia A Nikitina ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Clinical Signs ◽  
Total Content ◽  
Vaginal Wall ◽  
Vaginal Mucosa ◽  
Vaginal Secretion ◽  
Livestock Farming ◽  
Humoral Factors ◽  
Ig G ◽  
Pcr Test

Abstract Maintaining reproductive health is an urgent task in intensive livestock farming. The study of the influence of pathogens of the Mycoplasmataceae family on the microecology and protective properties of the vagina is of particular interest. The studies were carried out on non-pregnant cows 3–4 years old. Was formed 2 groups of 8 animals each. The first group is healthy cows in which the PCR test for Mycoplasma spp. was negative. The second group - cows with a positive PCR test and serological identification of M. bovigenitalium, without pronounced clinical signs of vaginitis. In both groups of animals, vaginal secretions were collected from the vaginal wall using a special spoon. In secret, by the method of radial immunodiffusion in a gel according to Mancini, the content of immunoglobulins of classes Ig G, Ig M, Ig A and secretory immunoglobulin A (sIgA) was determined, as well as the activity of lysozyme - by the nephelometric method. The study showed that the content of Ig G and the total content of immunoglobulins in the vaginal secretion in healthy cows and cows with mycoplasmosis did not have significant differences. The concentration of Ig A in cows with mycoplasmosis was 0.018±0.001 g/l, which was 25% less than in healthy cows, but it turned out to be insignificant (P &gt; 0.05). The content of Ig M and sIgA in secretion in healthy cows was 0.039±0.002 and 0.067±0.005 g/l, while in cows with mycoplasmosis it significantly increased by 38.5 and 43%, respectively. The activity of lysozyme in the secretion of healthy cows was 11.71±0.41%, while in infected cows it decreased by 2 times. With genital mycoplasmosis in cows, a quantitative redistribution of immunoglobulin classes and a decrease in lysozyme activity are observed in the vaginal secretion.

scholarly journals Development of self-reactive germinal center B cells and plasma cells in autoimmune FcγRIIB-deficient mice

2010 ◽  
Vol 207 (12) ◽  
pp. 2767-2778 ◽  
Author(s):  
Thomas Tiller ◽  
Juliane Kofer ◽  
Cornelia Kreschel ◽  
Christian E. Busse ◽  
Stefan Riebel ◽  
...  
Keyword(s):  
B Cells ◽  
Germinal Center ◽  
Plasma Cells ◽  
Cell Compartment ◽  
Fc Gamma Receptor ◽  
Gamma Receptor ◽  
Systemic Autoimmunity ◽  
Bone Marrow Plasma ◽  
Ig G ◽  
Deficient Mice

Abnormalities in expression levels of the IgG inhibitory Fc gamma receptor IIB (FcγRIIB) are associated with the development of immunoglobulin (Ig) G serum autoantibodies and systemic autoimmunity in mice and humans. We used Ig gene cloning from single isolated B cells to examine the checkpoints that regulate development of autoreactive germinal center (GC) B cells and plasma cells in FcγRIIB-deficient mice. We found that loss of FcγRIIB was associated with an increase in poly- and autoreactive IgG+ GC B cells, including hallmark anti-nuclear antibody–expressing cells that possess characteristic Ig gene features and cells producing kidney-reactive autoantibodies. In the absence of FcγRIIB, autoreactive B cells actively participated in GC reactions and somatic mutations contributed to the generation of highly autoreactive IgG antibodies. In contrast, the frequency of autoreactive IgG+ B cells was much lower in spleen and bone marrow plasma cells, suggesting the existence of an FcγRIIB-independent checkpoint for autoreactivity between the GC and the plasma cell compartment.

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