scholarly journals Antioxidant profile for rats with the closed cranial cerebral trauma in the treatment of cerebrogermum

2016 ◽  
Author(s):  
V. D. Lukianchuk ◽  
M. V. Ohloblina ◽  
O. V. Koveshnikov
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant System ◽  
Mechanical Damage ◽  
Control Group ◽  
Experiment Data ◽  
Antioxidant Profile ◽  
Cerebral Trauma ◽  
Main Components

The work is devoted to the research of the condition of the main components of the antioxidant system at the terms of the closed cranial cerebral trauma and at its pharmaceutical correction of Cerebrogermum.  It is determined that in case of using of Cerebrogermum the activity of superoxiddismutasa is rised in the blood serum in average on 35 % concerning the significations of the control group (the closed cranial cerebral trauma without treatment), on 72 hour of experiment the activity of this are fixed in the groups of both intact and reviewer rats. It does not also yield to reviewer preparation the ability of Cerebrogermum to resist to oxidizing stress because of the activity of catalasa. In relation to the estimation of antioxidant activity it considerably outweighs sizes for rats under influence of Cerebrogermum ( in average on 65 %) which are registered in the control group. The received in the experiment data give the basis to assert that the potential cerebral protector owns the exactly ability to resist to oxidative stress that is formed on a background of the mechanical damage of cerebrum.

scholarly journals Antioxidant system state in patients with multiple sclerosis current in therapy

2011 ◽  
Vol 57 (6) ◽  
pp. 661-670 ◽  
Author(s):  
L.P. Smirnova ◽  
N.V. Krotenko ◽  
E.V. Grishko ◽  
N.M. Krotenko ◽  
V.M. Alifirova ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant System ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Total Antioxidant Activity ◽  
Different Types ◽  
Total Antioxidant ◽  
High Level

Activity of erytrocyte glutationperoxidase (GP), glutationreductase (GR), glutationtransferase (GT), glucose-6-phosphatdehydrogenase (G6PDH), catalase and superoxiddysmutase (SOD), and also, the level of malonic dialdehyde (MDA) and total antioxidant activity of blood serum were studied in patients with different types of multiple sclerosis. Investigation of peripherical blood was carried out on first day of treatment and after standard therapy of copaxone. All MS patients had high level of MDA and activity of GP in erythrocytes in comparison with a control group. Other antioxidant enzymes of erythrocytes and total antioxidant activity of blood serum exhibited weak positive dynamics in patients with a relapsing remittance of multiple sclerosis (RRMS). Decrease of activity of antioxidant system in patients with secondary progression multiple sclerosis (SPMS) was more pronounced and remained unchanged after the treatment. This is consistent with the more severe clinical course of thise disease.

scholarly journals Interplay between antioxidant activity, health and disease

2019 ◽  
Vol 10 (1) ◽  
pp. 4893-4901 ◽  
Keyword(s):  
Analytical Chemistry ◽  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Blood Serum ◽  
Biological Fluids ◽  
Control Group ◽  
Pathological Conditions ◽  
Mediator System ◽  
Health And Disease ◽  
The Relationship

The article discusses the relationship between oxidative stress (OS) and pathological conditions, the possibilities and benefits of estimating OS considering the integral antioxidant activity (AOA) as an OS criterion, and using a simple accessible hybrid potentiometric method (HPM) with a mediator system for AOA monitoring. The results of AOA of blood serum in healthy volunteers and patients with various diseases are presented. Preliminary reference values are found. The lower levels of AOA of blood serum in patients with different diseases in comparison with the control group are observed. The potential mechanisms of changes in the AOA levels and it’s clinical significance are discussed from the position of biointerfaces interplay. With AOA equal or greater than 1.40 mmol-eq l–1 the person is healthy, the range from 0.95 to 1.40 mmol-eq l–1 indicates that the patient is at risk and needs to undergo a further medical examination. When AOA blood serum is below 0.95 mmol-eq l–1, detailed diagnostics and relevant treatment are required. The findings allow suggesting that the approach determine antioxidant/oxidant activity of biological fluids holds considerable promise for monitoring OS; it opens up new opportunities in expanding the use of analytical chemistry in medicine.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Diabetes Type 2 ◽  
Male Rats ◽  
Control Group ◽  
Standard Drug ◽  
High Fructose ◽  
Group A ◽  
Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

scholarly journals EFFECT OF STREBLUS ASPER LEAVES ON LOCOMOTION, ANXIETY AND COGNITION IN RATS

2019 ◽  
pp. 98-101
Author(s):  
SACHIN NEEKHRA ◽  
HIMANI AWASTHI ◽  
DCP SINGH
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Muscle Relaxant ◽  
Significant Variation ◽  
Control Group ◽  
Hydroalcoholic Extract ◽  
Central Nervous System Disorders ◽  
Inhibition Concentration ◽  
Locomotor Activities ◽  
Streblus Asper

Objective: The current study deals with the evaluation of neuropharmacological activities of hydroalcoholic extract of the plant Streblus asper. Methods: Hydroalcoholic extract of S. asper leaves was administered to animals at the dose of 200 and 400 mg/kg p.o., respectively. The neuropharmacological activities, namely, anxiolytic, muscle-relaxant, nootropic, and locomotor activities of hydroalcoholic extract of S. asper leaves were evaluated. The antioxidant activity of the hydroalcoholic extract of S. asper leaves was also investigated. Results: The dose 400 mg/kg p.o. of hydroalcoholic extract indicated significant variation with control group on neuropharmacological activity, especially nootropic and locomotion, whereas the mentioned dose did not show a significant effect on anxiolytic and muscle-relaxant activities. Percentage scavenging activities and inhibition concentration (IC50) were reported as 63.132 at 100 μg/ml and 35.33, respectively. Conclusion: It was found that hydroalcoholic extract of S. asper leaves can treat central nervous system disorders caused by oxidative stress.

scholarly journals Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children’s Saliva

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Heitor Ceolin Araujo ◽  
Ana Cláudia Melo Stevanato Nakamune ◽  
Wilson Galhego Garcia ◽  
Juliano Pelim Pessan ◽  
Cristina Antoniali
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Antioxidant System ◽  
Oxidative Stress Biomarkers ◽  
System Activity ◽  
Enzymatic Antioxidant ◽  
Stress Biomarkers ◽  
Salivary Biomarkers ◽  
Carious Lesions

Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage.

The state of the blood antioxidant system in the patients presenting with acromegaly

2015 ◽  
Vol 61 (2) ◽  
pp. 8-11
Author(s):  
M V Faassen ◽  
M S Pankratova ◽  
N N Molitvoslovova ◽  
A A Baizhumanov ◽  
S S Kovalenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Antioxidant System ◽  
The State ◽  
Total Antioxidant Activity ◽  
Sod Activity ◽  
Protein Thiols ◽  
Total Antioxidant

The state of the blood antioxidant system in the patients presenting with acromegaly. The objective of the present study was to evaluate the total antioxidant activity (TAA) of blood plasma, the levels of non-protein thiols and 2-thyobarbituric acid-active products (TBA-AP), superoxide dismutase (SOD) and catalase (CAT) activities as well as ceruloplasmin (CP) level in the patients presenting with acromegaly. It was shown that plasma TAA and SOD activity in this patients was on the average 20 and 30% lower respectively than in the control subjects. At the same time, the TBA-AP and CP levels increased by 50 and 40% respectively. These data suggest the development of oxidative stress in the acromegalic patients.

scholarly journals Clinical efficiency of antioxidant therapy in patients with liver cirrhosis and acute blood loss

2021 ◽  
pp. 70-80
Author(s):  
D. A. Evseenko ◽  
Z. A. Dundarov
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Uric Acid ◽  
Liver Cirrhosis ◽  
Mortality Rate ◽  
Blood Loss ◽  
Blood Serum ◽  
Acute Blood Loss ◽  
Treatment Protocols ◽  
Clinical Efficiency

Objective: to determine the clinical efficiency of antioxidant therapy in patients with liver cirrhosis and acute blood loss.Material and methods. The study included 286 patients with liver cirrhosis and acute blood loss of varying degrees of severity. The patients were divided into two series of studies. In the first series, the patients received the therapy according to the clinical diagnostic and treatment protocols, in the second series 1 according to the same protocols in combination with an intravenous administration of the antioxidant complexes “Reamberin” and “Fluimucil”. After 48 hours from admission, the antioxidant status of blood serum, the blebbing index of lymphocytes, the concentration of uric acid, and the level of hemoglobin were analyzed. The indicators of the clinical efficiency of the treatment were determined: risk of absolute mortality, risk of relative mortality, the number needed to treat.Results. The blood serum of the patients from the first series of studies was characterized by pronounced prooxidant activity, high values of the lymphocyte blebbing index, and a small amount of uric acid, which was indicative of the development of oxidative stress. The mortality rate in this series of studies was 26.0 %. The patients of the second series of studies were characterized by pronounced antioxidant activity of blood serum, low values of the lymphocyte blebbing index, and high concentrations of uric acid, which indicated a decrease in the activity of systemic oxidation processes. The mortality rate in this series of studies was 18.2 %. The duration of the treatment decreased from 16.5 days (first series) to 13.0 days (second series).Conclusion. The combined use of the “Reamberin” and “Fluimucil” complexes as part of the therapy according to the clinical diagnostic and treatment protocols leads to a relief of oxidative stress in patients with liver cirrhosis complicated by acute blood loss. The blood serum of these patients is characterized by pronounced antioxidant activity, low values of the lymphocyte blebbing index, and a high concentration of uric acid, which reduces the risk for the development of multiple organ failure syndrome. This therapy decreases the mortality rate to 18.2 % and the duration of the treatment to 13.0 days.

scholarly journals Protective Role of Ce Nanoparticles Against the Hepatotoxicity Induced by Exposure to Paraquat

2018 ◽  
Vol 6 (2) ◽  
pp. 37-43
Author(s):  
Hamid Heidary Dartoti ◽  
Farzin Firozian ◽  
Sara Soleimani Asl ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Role ◽  
Male Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Serum Samples ◽  
Tissue Samples ◽  
Total Antioxidant ◽  
Group 2

Objectives: The present study aimed to investigate the antioxidant activity of cerium oxide nanoparticles (CeNPs) against paraquat (PQ)-induced liver injury in rats. Methods: Thirty-two male rats were divided into four 8-member groups and treated intraperitoneally with PQ and/or CeNPs for 14 days. Group 1 received PQ (5 mg/kg/d), group 2 received CeNPs (15, 30, and 60 mg/kg/d), group 3 received a combination of PQ (5 mg/kg/d) and CeNPs (15, 30, and 60 mg/kg/d), and group 4 (control group) received saline solution. Serum samples along with liver tissue samples were collected from all the rats. Oxidative stress (OS) biomarkers including total antioxidant capacity, lipid peroxidation, total thiol groups, DNA damage, and nitric oxide levels were determined. Histological samples were also analyzed using hematoxylin and eosin staining slides. Results: Levels of oxidative stress and hepatic tissue damage were significantly higher in the PQ group compared to the control group. CeNPs at a dose of 15 mg/kg showed the antioxidant activity and compromised the PQ-induced damage. Conclusion: In the scenario tested in this study, CeNPs could reduce the levels of OS, as well as hepatic damage induced by PQ.

scholarly journals Evaluation of the state of the pro-oxidant/antioxidant system in children with hereditary spherocytosis

2021 ◽  
pp. 55-61
Author(s):  
E. F. Mitsura ◽  
I. A. Novikova ◽  
T. S. Petrenko ◽  
K. S. Makeeva ◽  
L. I. Volkova
Keyword(s):  
Oxidative Stress ◽  
Antioxidant System ◽  
Antioxidant Status ◽  
Hereditary Spherocytosis ◽  
The State ◽  
Control Group ◽  
Healthy Children ◽  
Gender And Age ◽  
Severity Of The Disease

Objective: to assess the state of the pro-oxidant/antioxidant system in children with hereditary spherocytosis (HS) depending on its severity.Material and methods. The study involved 44 HS patients at the age from 1 to 17 who were divided into 2 groups depending on the disease severity: mild course (n = 24) and moderate or severe course (n = 20). The control group included 23 practically healthy children who were comparable with the main group by gender and age. The state of the pro-oxidant/antioxidant balance of blood plasma was assessed by the method of luminol-dependent chemiluminescence (LDCL) with the determination of the maximum luminescence intensity (Imax, %) and the light sum of chemiluminescence (S, %). The activity of superoxide dismutase (SOD) and catalase was determined in the erythrocytes of the examined children.Results. On average, the parameters of the pro-oxidant/antioxidant status in the HS patients significantly differed from those of the control group (p <0.05), which corresponded to moderately pronounced oxidative stress. The activity of SOD and catalase in the erythrocytes of the patients was higher as compared with that of the control group (p = 0.0001 and p <0.0001, respectively). The comparison of the severity of oxidative stress depending on HS severity has determined that the degree of stress was more pronounced in patients with moderate or severe course of the disease (p <0.05).Conclusion. HS patients develop oxidative stress (decreased activity of the antioxidant system associated with increased accumulation of prooxidant substances), the degree of which is higher in patients with a severe course of the disease. This allows of considering plasma LDCL indicators as an additional marker for the assessment of the severity of the disease and of justifying the necessity to include antioxidants in the HS treatment regimen.

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