scholarly journals Особливості процесів ліпопероксидаціїв порожній кишціпри резекції різних об’ємів паренхіми печінки

2018 ◽  
Author(s):  
M. S. Hnatjuk ◽  
L. V. Tatarchuk
Keyword(s):  
Lipid Peroxidation ◽  
Endothelial Cells ◽  
Epithelial Cells ◽  
Liver Parenchyma ◽  
Relative Volume ◽  
Male Rats ◽  
Diene Conjugates ◽  
Experimental Animals ◽  
Peroxidation Processes ◽  
Smooth Myocytes

Introduction. Resections of large volumes of liver parenchyma are complicated by postresection portal hypertension, which leads to structural and functional changes in the organs of the basin portal hepatic vein. The features of the remodeling of the jejunum structures and the features of the processes of lipid peroxidation after resection of different volumes of liver parenchyma were not adequately investigated.The aim of the study – to learn the features of lipid peroxidation processes in the jejunum of experimental animals after resection of different volumes of liver parenchyma.Research Methods. The studies were performed on 36 white male rats, which were divided into 3 groups. The first group consisted of 12 intact experimental animals (control), 2nd – 12 animals, which removed the left side lobe of the liver, which was 31.5% of its parenchyma, 3rd – 12 rats after resection of the left and right lateral lobes, that is 58, 1% of liver parenchyma. One month after the beginning of the experiment, euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia. In quantitative evaluation of lipid peroxidation processes, the content of diene conjugates and active products of thiobarbituric acid in the wall jejunum homogenates were determined. Sections from the jejunum were fixed in a 10% neutral formalin solution, and after conducting, the ethyl alcohol of increasing concentration was placed in paraffin. Histologic sections 5–7 мm thick after deparaffinization were stained with hematoxylin-eosin, for van Gizon, Mallory, Weigert, and toluidine blue. Gistostereometrically there were determined the relative volumes of damaged endothelial cells, epithelial cells, smooth myocytes, relative volume of stroma in the muscle. A correlation analysis was carried out between biochemical and histostereometric indices with the definition of the coefficient (r) of correlation. Quantitative values were processedstatistically.Results and Discussion. It was established that in conditions of the simulated experiment one month after the resection of the liver the expressed processes of lipid peroxidation occurred in the intestine. Thus, after removal of 31.5% of the liver parenchyma, the content of diene conjugates increased in 1.9 times after resection of 58.1% of liver parenchyma – 3.4 times, the concentration of active products of tiobarbituric acid, respectively, in 2.1 and 6.7 times. The obtained and analyzed indicators testify that at removal of significant volumes of liver parenchyma processes of peroxide oxidation of lipids, which depend on the removed volume of a liver, are substantially increased. A relative volume of damaged epithelial cells of the mucous membrane of the jejunum in the 2nd group of observations increased in 2.6 times, endothelial cells – 2 times, smooth myocytes – 1.86 times, stroma in the muscle of the jejunum– 12.7%. After resection of 58.1% of liver parenchyma,the investigated morphometric parameters increased by 30.6; 19.0; 11.8 and 2.4 times. Correlation connections between the concentrationlipid peroxidation products and relative volumes of damaged endothelial cells, epithelial cells, smooth myocytes and stroma in the wall of the jejunum were positive and significant and intensified when 58.1% of liver parenchyma were removed and ranged from +0.53 to +0.84,Conclusions. Lipid peroxidation plays an important role in the adaptive-compensatory processes of the jejunumafter resection of different volumes of liver parenchyma. The degree of morphological changes in the jejunum correlates with the concentration oflipid peroxidation products and depends on the removed volume of liver parenchyma.

scholarly journals Стан антиоксидантної системи експериментальних тварин при пошкодженні дванадцятипалої кишки за умов пострезекційної портальної гіпертензії

2018 ◽  
Author(s):  
M. S. Hnatjuk ◽  
L. V. Tatarchuk
Keyword(s):  
Portal Hypertension ◽  
Epithelial Cells ◽  
Antioxidant System ◽  
Liver Parenchyma ◽  
The State ◽  
Male Rats ◽  
Antioxidant Defence ◽  
Experimental Animals ◽  
Functional Changes ◽  
Smooth Myocytes

Introduction. The resections of large volumes of the liver leads to postresection portal hypertension that results in structural-functional changes in the organs of portal vena system. The features of remodeling structures of duodenum and state of the antioxidant system of organism in the conditions of postresection portal hypertension are not studied yet. The aim of the study – to learn the state of the antioxidant system for experimental animals at the structural-functional changes of duodenum in the conditions of postresection portal hypertension. Research Methods. The studies were performed on 39 white male rats, which were divided into 3 groups. The first group consisted of 15 intact experimental animals (control), 2nd – 12 animals, which eliminated 42 % of liver parenchyma, 3rd – 12 rats after resection of 58.1 % of liver parenchyma. One month after the beginning of the experiment, euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia. In the blood of experimental animals, indicators characterizing the state of oxidative protection were determined – the activity of superoxidedismutase, catalase and ceruloplasmin. Sections of the duodenum were fixed in a 10 % neutral formalin solution and, after passing through the ethyl alcohol of increasing concentration, were placed in paraffin. Histologic sections 5–7 мm thick after deparaffinization were stained with hematoxylin-eosin, for van Gizon, Mallory, Weigert, and toluidine blue. Gistostereometrically determined the relative volumes of damaged endothelial cells, epithelial cells, smooth myocytes. A correlation analysis was carried out between biochemical and histogeometric indices with the definition of the coefficient (r) of correlation. Quantitative values were processed statistically. Results and Discussion. It is set that in the conditions of the modelled experiment activity of superoxidedismutase at the resection of 42 % parenchyma of liver statistically for certain (р<0.001) diminished on 15.5 % and at removal of 58.1 % parenchima of liver – on 25 %; activity of catalase – accordingly on 22.2 and 38.8 % and activity of ceruloplasmin – on 15.3 and 21.3 %. Received and the analyzed indexes testify that at resection of considerable volumes (42; 58.1 %) parenchyma of liver antioxidant defence of organism gets worse substantially and depends on removal at volumes of liver. A relative volume of the damaged endotheliocytes is in 2 to the group of supervisions with high authenticity (р<0.001) grew in 8.4 times, epithelial cells – in 18.9 times, and smooth myocytes – in 12 times. After the resection of 58.1 % parenchyma of liver the investigated morphometric parameters increased accordingly in 15.8; 31.4 and 18.1 times. Correlation connections between the indexes of antioxidant defence and relative at volumes of the damaged endotheliocytes, epithelial cells and smooth myocytes in duodenum at resection 42 % parenchima of liver were opposite moderate and significance (r=–0.48ч–0.56) at resection 58.1 % of liver – significance (r=–0.72÷-0.83). Conclusions. The obtained results indicate that antioxidant protection of the organism plays an important role in the adaptive-compensatory processes of the duodenum after resection of different volumes of liver parenchyma. The degree of antioxidant defense of the organism depends on the removed volume of liver parenchyma and the number of damaged structures of the duodenum.

scholarly journals ПОРУШЕННЯ ВСМОКТУВАЛЬНОЇ ФУНКЦІЇ ТОНКОЇ КИШКИ ПРИ ПОСТРЕЗЕКЦІЙНІЙ ПОРТАЛЬНІЙ ГІПЕРТЕНЗІЇ

2018 ◽  
Author(s):  
M. S. Hnatiuk ◽  
L. V. Tatarchuk
Keyword(s):  
Small Intestine ◽  
Portal Hypertension ◽  
Epithelial Cells ◽  
Liver Parenchyma ◽  
Relative Volume ◽  
Male Rats ◽  
Morphometric Parameter ◽  
Lateral Part ◽  
Chemical Agent ◽  
Absorption Function

The liver resection is widely used in modern surgical clinics. Removal of large volumes of liver leads to postresection portal hypertension, which is complicated by bleeding from varicose veins of the esophagus, stomach, rectum, ascites, splenomegaly, secondary hypersplenism, parenchymal jaundice, and portosystemic encephalopathy. The widespread prevalence of this pathology, high mortality from its complications indicates that it is an important medical and social problem.The aim of the study – to learn the features of the suction function of the small intestine at postresection portal hypertension.Materials and Methods. The research was conducted on 45 laboratory-sexually mature white male rats, which were divided into 3 groups. The 1 group consisted of 15 intact virtually healthy animals, 2–15 rats after resection of the left lateral part – 31.5 % of liver parenchyma, 3–15 animals after removal of the right and left lateral parts of the liver (58.1 %). One month after the start of the experiment, the suction function of the small intestine was investigated for the D-xylose test. It is specified chemical agent is an inert carbohydrate which is absorbed by passive diffusion. D-xylose is not metabolized in the human body and animals, is excreted in the urine, fully and adequately reflects the suction function of the small intestine. From the small intestine made histological micropreparations. Morphometrically determined the height of the epitheliocytes of the mucousa of the intestine, the diameter of their nuclei, nuclearcytoplasmic relations in them, the relative volume of their damage. Quantitative indicators were processed statistically.Results and Discussion. It was found that one month after resection of 31.5 % of liver parenchyma, the content of D-xylose in urine decreased by only 5.1 %, indicating a slight disruption of the absorption function of the small intestine, and resection 58.1 % of the liver decreased of D-xylose in urine by 28.6 %. The latter figure testified to a marked violation of the suction function of the small intestine. One month after resection, 58.1 % of liver parenchyma decreased the height of epithelial cells by 19.4 % compared with the same control quantitative morphological parameters, and the diameter of their nuclei – only by 2.3 %. Non-uniform disproportionate changes in spatial characteristics of the nucleus and cytoplasm of the epithelial cells resulted in marked violations of nuclear-cytoplasmic relations in these cells. At the same time, the indicated morphometric parameter is statistically significantly (p <0.001) increased by 47.9 %, which indicated an alteration of structural cellular homeostasis. Relative volume of damaged endothelial cells a month after removal of 58.1 % of liver parenchyma increased 21.3 times. Significant correlation between the concentration of D-xylose in urine, nuclear-cytoplasmic ratios in epitheliocytes and the relative volume of damaged epithelial cells was found, which allowed to confirm the close relationship between structural changes in mucosa of the intestine and violation of the suction function in the simulated experimental conditions. Consequently, significant resection of the liver parenchyma leads to posresection portal hypertension, severe lesions of the mucosa of the intestine, which significantly impairs the suction function of the small intestine.Conclusions. Resection of 58.1 % of liver parenchyma leads to postresection portal hypertension and severe structural rearrangement of the epitheliocytes of the mucosa of the small intestine, which is confirmed by a disturbance in their cellular structural homeostasis and a significant increase in the relative volume of their damage, which was complicated by a significant violation of the suction function of the small intestine.

scholarly journals The Damage Sensory Molecule TRPA1 Positively Regulates Endogenous Thymic Regeneration after Damage

Blood ◽  
2017 ◽  
Vol 130 (Suppl_1) ◽  
pp. 67-67
Author(s):  
Kimon Argyropoulos ◽  
Enrico Velardi ◽  
Jennifer Tsai ◽  
Amina Lazrak ◽  
Lorenz Jahn ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Lipid Peroxidation Products ◽  
Positive Effects ◽  
Damage Sensing ◽  
Cell Counts ◽  
Thymic Regeneration

Abstract The thymus is extremely sensitive to exogenous insults but has a remarkable capacity to regenerate which is lost with age. Reactive oxygen species (ROS) accumulate early after tissue damage and despite their toxic potential, ROS and their byproducts (such as lipid peroxidation products-LPPs) can act as regeneration signals by activating membrane or intracellular sensors and subsequent stress-response signalling pathways. Using Sublethal Total Body Irradiation (SL-TBI) as a model of acute thymic injury, we found a rapid accumulation of thymic ROS as well as lipid peroxidation products on cell membranes after SLTBI (Figure 1A&B). The damage-sensing ion channel Transient Receptor Potential cation channel family A member 1 (TRPA1) represents one of the major damage sensing receptors that can mediate cellular responses to oxidative stress mediators, such as LPPs. Using immunofluorescence (IF) microscopy we found that TRPA1 is enriched in the thymic medulla. Interestingly, although TRPA1 has been classically identified in nociceptive fibers, the major TRPA1 expressing structures in the thymus were not nerve fiber terminals, but primarily thymic endothelial cells (Figure1C), fibroblasts and subsets of epithelial cells. We have recently demonstrated that thymic endothelial cells can regulate regeneration through secretion of BMP-4, which can enhance Foxn1 expression and proliferation of thymic epithelial cells. In order to assess the functional role of TRPA1 in thymic regeneration after injury, we utilized TRPA1 knockout (TRPA1-/-) mice and quantified thymic reconstitution after SL-TBI. TRPA1-/- mice had significantly lower thymic cellularity compared to their age- and sex-matched WT controls, suggesting an association between TRPA1 deficiency and delayed endogenous thymic recovery (Figure 1D). The major deficit in thymocyte counts primarily affected double negative-4 (DN4), double positive (DP) and CD4+ single positive (SP-CD4+) thymocyte numbers. The thymic stroma of TRPA1-/- mice had lower endothelial cell and fibroblast counts (Figure 1D). In accordance with these findings drinking water administration of the TRPA1 agonist Allyl-Isothiocyanate (AITC), resulted in enhanced thymic regeneration after radiation exposure. Besides its positive effects on thymocyte counts, AITC significantly augmented endothelial cell counts after irradiation (Figure 1E). In conclusion these results suggest that TRPA1 plays a non-redundant role in thymic regeneration and that exogenous TRPA1 stimulation can enhance immune recovery after damage. Disclosures van den Brink: Seres: Research Funding; Jazz Pharmaceuticals: Consultancy; PureTech Health: Consultancy; Therakos Institute: Other: Speaking engagement.

scholarly journals Динаміка зміни показників системи ПОЛ-АОЗ, вуглеводного та ліпідного обміну в крові експериментальних щурів при переході гострого панкреатиту у хронічну форму

2012 ◽  
Vol 3 (2) ◽  
pp. 48-53
Author(s):  
V. A. Makarchuk ◽  
G. О. Ushakova
Keyword(s):  
Lipid Peroxidation ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Main Duct ◽  
Experimental Animals ◽  
Pancreatic Tail ◽  
Carbohydrate And Lipid Metabolism ◽  
Antioxidant Protection System ◽  
Lipid Peroxidation Inhibition ◽  
Significant Intensification

In consequence of ligation of the main duct in pancreatic tail section of male rats the acute pancreatitis had developed but it gradually turned into a chronic form. In the blood of experimental animals processes of lipid peroxidation (LPO), the state of antioxidant protection system (AOP), carbohydrate and lipid metabolism were studied. In rats with chronic pancreatitis the significant intensification of lipid peroxidation, inhibition of antioxidant system, hyperglycaemia, and hyperlipidaemia were found.

scholarly journals Changes of Prooxidant-Antioxidant Systems in Experimental Acute Pancreatitis

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
Viktoria Cherkasova ◽  
Luibomyr Zaiats
Keyword(s):  
Lipid Peroxidation ◽  
Acute Pancreatitis ◽  
White Male ◽  
Thiobarbituric Acid ◽  
Activity Level ◽  
Male Rats ◽  
Antioxidant Systems ◽  
Diene Conjugates ◽  
Pronounced Increase ◽  
Oxidation Rates

Mortality in acute destructive pancreatitis, despite the development and introduction of new methods of treatment, remains stable high and in severe forms reaches 25-85%. Activation of neutrophils and macrophages in acute pancreatitis leads to an "oxygen burst", which is closely linked with the activation of lipid peroxidation.Goals. The purpose is to establish dynamic changes in the indexes of prooxidant-antioxidant systems in acute L-arginine-induced pancreatitis.Materials and methods. The study was performed on 62 white male rats of Wistar line weighing 180-220g, with modeled acute pancreatitis. Blood for analysis have been taken: the blood serum on 12, 24, 48 and 72 hours of experiment to determine the activity level of thiobarbituric acid products, diene conjugates, catalase and lactate for assessment of the intensity of oxidative stress and antioxidant systems.Results. The obtained results of the study showed that acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation processes (LPO). Revealed that the most pronounced increase in all blood parameters is observed 24 hours after the beginning of the study. A significant increase in the active products of tiobarbituric acid (TBA-AP) and diene conjugates (DC) was detected - 1.98 and 2.7 times, respectively, and 2.2 times the growth of catalase (CT). At the next stage of the experiment there is a slowdown in the rate of LPO, as evidenced by the following values. Thus, for 48 years in the 3rd group: TBA-AP - they increased by 5.1% (p> 0.05), DC - by 3.3% (p> 0.05), and the level of CT - by 43.4% (P <0.05), compared with data for 24 hours. It is important to note that at 72 hours, the CT level decreased by 23.3% (p> 0.05), which may indicate an exhaustion of antioxidant systems. Indicators of LPO on 72 hours compared with 48 hours in group III: TBA-AP - increased by 1.7% (p> 0.05), DC - by 5.7% (p> 0.05).Conclusions. Acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation-oxidation processes that can potentiate the development of multiple organ failure in pancreatic inflammation. The most pronounced changes in lipid peroxidation-oxidation rates are observed for 24 hours of study.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals Metabolic processes in the small intestine wall, heart and liver in experimental intake of carrageenan

2017 ◽  
Author(s):  
O. M. Kopanytsia ◽  
M. I. Marushchak ◽  
A. A. Shcherbatyy
Keyword(s):  
Lipid Peroxidation ◽  
Small Intestine ◽  
Free Radical ◽  
Water Solution ◽  
Free Radical Oxidation ◽  
The Body ◽  
Male Rats ◽  
Free Access ◽  
Metabolic Processes ◽  
Experimental Animals

Introduction. In the scientific literature there is a large number of publications on the chemical modification of carrageenans, the relationship between the chemical structure and their biological activity, and new opportunities for their usage in medicine. That is whyit is modern to study the properties and the state of organs and systems of the body in case of carrageenan intake.The aim of the study – to learn the indices of metabolic processes in the wall of the small intestine, myocardium and liver of experimental animals using 1% carrageenan solution.Research Methods. The study was carried out on 24 white non-linear male rats. The animals of the experimental group were provided with free access to a 1.0% of carrageenan solution for 1 month. In the selected samples of the small intestine, heart and liver was evaluated the intensity of the processes of lipid peroxidation by the content of hydroperoxides and TBA-active products.Results and Discussion. It was found that the intake of a 1% solution of κ-carrageenan led to activation of lipid peroxidation both in the wall of the small intestine and in the tissues of the liver and myocardium, however, their intensity was different. The lipoperoxidation activity was highest in the small intestine wall, while the hydroperoxides and TBA-active products were less in the myocardial tissues. So, the oral usage of carrageenan in the form of a 1% water solution has a direct and indirect damaging effect on the free radical processes in the body.Conclusions. Oral administration of a 1% carrageenan solution in experimental animals leads to statistically significant activation of free radical oxidation processes in the small intestine wall, heart and liver tissues, characterized by an increase in the content of both primary and secondary products of lipoperoxidation (p<0.05).

scholarly journals Effect of diagnostic ultrasound on corneal apoptosis in rats

2020 ◽  
Vol 19 (9) ◽  
pp. 1947-1951
Author(s):  
Cong Liu ◽  
Xin Yu ◽  
Yun Kou
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Stromal Cells ◽  
Irradiation Time ◽  
Diagnostic Ultrasound ◽  
Male Rats ◽  
Control Group ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Apoptosis Rate

Purpose: To investigate the effect of diagnostic ultrasound on corneal apoptosis in rats.Methods: 24 male rats were randomly divided into 4 groups: control group, 10, 20 and 30 min group. The eyeballs of rats were irradiated  continuously for different time lengths by Siemens ACUSON S2000 color Doppler ultrasound diagnostic instrument. 24 hours later, the animals were killed and the corneas were taken for Tunel apoptosis detection. The apoptosis rates of corneal epithelial cells, stromal cells and endothelial cells were calculated.Results: Apoptotic cells were detected in corneal epithelial cells, stromal cells and endothelial cells of normal rats. There was no significant difference between the 10 min group and the control group (P>0.05). The apoptosis rate of 20 min and 30 min groups was significantly higher than that of the control group. With the extension of irradiation time, the apoptosis rate of corneal epithelial cells, stromal cells and endothelial cells increased.Conclusion: 20 min of rat eyeball irradiated by diagnostic ultrasound can increase the apoptosis of corneal cells, and the apoptosis is aggravated with the prolongation of ultrasound irradiation time. Keywords: Cornea; Ultrasonography; Apoptosis; Epithelial cells

scholarly journals CORRECTION OF PROCESSES OF LIPID PEROXIDATION AND ENDOGENIC TOXICITY WITH EXTRACT OF GERANIUM PALUSTRE HERB AS A DENTAL FILM IN CONDITIONS OF EXPERIMENTAL STOMATITIS

2018 ◽  
pp. 17-20
Author(s):  
M.Yu. Khavrona ◽  
I.L. Benzel ◽  
R.M. Fedin ◽  
O.R. Pinyazhko ◽  
O.P. Khavrona
Keyword(s):  
Lipid Peroxidation ◽  
Therapeutic Effect ◽  
Oral Mucosa ◽  
Biologically Active ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Experimental Animals ◽  
Middle Molecules ◽  
Peroxidation Processes ◽  
Ulcerative Stomatitis

The development of stomatitis is characterized by the appearance of oxidative stress, which is marked by an increase in the intensity of peroxide lipid oxidation processes, resulting in metabolic disturbance in oral mucosa. As a result, toxic substances accumulate and endogenic toxicity develops. Thus, correction of pathological processes in the oral mucosa with antioxidant medication enables to optimize the intensity of inflammation, shorten the period of catabolic phase and accelerate the regeneration. Extract of Geranium palustre herb as a dental film was used to reduce the intensity of processes of lipid peroxidation and endogenous intoxication. This treatment method enables to release an active substance from the drug locally and to provide optimal therapeutic effect. The efficacy of the experimental medication was studied in the dynamics of experimental ulcerative stomatitis development in rabbits. The experimental model of ulcerative stomatitis in animals was induced with a burn method. For the experiment, the animals were divided into 2 groups: control group, where treatment was not performed, and experimental group, where animals were treated by applying a dental film with Geranium palustre extract. The monitoring was conducted on the 3rd, 5th, 7th and 10th days of the experiment, which are key stages of healing. During these periods, blood was taken under anesthesia from the auricular vein of each experimental animal. Dynamics of prooxidant processes was detected by the level of lipid hydroperoxides and malondialdehyde, and the level of endogenic toxicity by the content of middle molecules. Statistic processing of the obtained results of investigation was performed using personal computer with installed proper software package StatSoft Statistica 10. Reliable difference was considered р<0.05. It has been shown that extract of Geranium palustre herb, as a dental film, has antioxidant properties, which are effectively reducing the intensity of lipid peroxidation processes. It should be mentioned that the content of lipid hydroperoxides and final product of lipid peroxidation processes – malondialdehyde – in experimental animals’ blood was statistically lower in all periods of investigation than in control group, where treatment was not performed. The obtained results show that higher inhibiting effect of experimental medication is marked during the final stage of lipid peroxide oxidation than during the initial stage. As the result showed, the development of endogenic toxicity was observed in both groups and the highest indices were recorded at the peak of the inflammatory process on the third day of the experiment. Dynamics of middle molecules content shows that application of Geranium palustre extract, as a dental film, decreases this index during all investigated periods and reduces intoxication level in experimental animals. Thus, application of dental film in stomatitis treatment enables to release medication directly into the damaged area, which significantly increases therapeutic effect of biologically active substances of Geranium palustre phytocomplex, and rapid absorption of the film in the oral cavity provides easy use. Considering efficacy, non-toxicity and availability of the investigated medication, the expediency of its application in dental practice for the treatment of stomatitis and other inflammatory diseases of the oral mucosa may be considered.

scholarly journals THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN WOMEN WITH PRIMARY VARICOSE VEINS OF THE PELVIC DEPENDING ON THE STAGE OF THE DISEASE

2018 ◽  
Vol 73 (4) ◽  
pp. 229-235 ◽  
Author(s):  
Lyubov’ I. Kolesnikova, ◽  
Andrey A. Semendyaev ◽  
Dmitriy A. Stupin ◽  
Marina A. Darenskaya ◽  
[Lyudmila A. Grebenkina ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Varicose Veins ◽  
Reproductive Age ◽  
Background Information ◽  
Control Group ◽  
Maximum Increase ◽  
Diene Conjugates ◽  
Primary Varicose Veins ◽  
Peroxidation Processes

Background: Information about involvement of general nonspecific reactions, in particular lipid peroxidation processes, in the progression of varicose veins of the pelvic (VVP) in women is still too scarce. Aims: To study the intensity of processes of lipid peroxidation and the activity of components of the antioxidant system in women with primary varicose veins of the pelvic at different stages of the disease. Materials and methods: 167 women of reproductive age were examined ― 137 with VVP and 30 made up a control group. All patients with VVP were divided into 3 groups depending on stages of the disease. Spectrophotometric and fluorometric methods of investigation were used. The study was conducted during 2012−2017. Results: It was revealed that the level of primary products of lipid peroxidation, diene conjugates, increased statistically significantly according to the stage of the disease by 1.25 times (in the 1st stage), 1.51 times (in the II stage) and 1.59 times (in the III stage) values. Changes in the content of final TBA-active products showed similar changes-an increase in the mean values for all stages of the disease relative to control (in 1.24, 1.17, and 1.77 times, respectively). Activity of glutathione peroxidase increased in stage 2 of VVP (1.19 times), with the maximum increase in stage III (1.42 times); activity of glutathione-S-transferase increased 1.18 times in the II stage of the disease. The concentration of GSH in the clinical groups was characterized by lower values with respect to the control (by 1.22 times in the 1st stage, in 1.64 times in the II stage), with the maximum decrease of this parameter in the III stage of VVP (3.67 times). The level of catalase activity increased in the I stage of VVP ― by 1.18 times and decreased in the III stage ― by 1.14 times with respect to the control. The activity of SOD showed similar changes with catalase ― in the form of increased activity at the 1st stage (1.35 times higher) and decreased values for II (1.35 times lower) and III (1.65 times lower) for the stages of VVP to the values of control. Conclusions: At progression of primary VVP in women (from the initial stage to the 3rd stage of the disease), there is an increase in imbalance in the lipid peroxidation − antioxidant defense system. Moreover, if the compensatory increase in activity of antioxidant enzymes is registered at stage 1 of the disease, then the most of the antioxidant defense factors decreases as relative to control values, and the initial stages of the disease.

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