scholarly journals CORRECTION OF PROCESSES OF LIPID PEROXIDATION AND ENDOGENIC TOXICITY WITH EXTRACT OF GERANIUM PALUSTRE HERB AS A DENTAL FILM IN CONDITIONS OF EXPERIMENTAL STOMATITIS

2018 ◽  
pp. 17-20
Author(s):  
M.Yu. Khavrona ◽  
I.L. Benzel ◽  
R.M. Fedin ◽  
O.R. Pinyazhko ◽  
O.P. Khavrona
Keyword(s):  
Lipid Peroxidation ◽  
Therapeutic Effect ◽  
Oral Mucosa ◽  
Biologically Active ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Experimental Animals ◽  
Middle Molecules ◽  
Peroxidation Processes ◽  
Ulcerative Stomatitis

The development of stomatitis is characterized by the appearance of oxidative stress, which is marked by an increase in the intensity of peroxide lipid oxidation processes, resulting in metabolic disturbance in oral mucosa. As a result, toxic substances accumulate and endogenic toxicity develops. Thus, correction of pathological processes in the oral mucosa with antioxidant medication enables to optimize the intensity of inflammation, shorten the period of catabolic phase and accelerate the regeneration. Extract of Geranium palustre herb as a dental film was used to reduce the intensity of processes of lipid peroxidation and endogenous intoxication. This treatment method enables to release an active substance from the drug locally and to provide optimal therapeutic effect. The efficacy of the experimental medication was studied in the dynamics of experimental ulcerative stomatitis development in rabbits. The experimental model of ulcerative stomatitis in animals was induced with a burn method. For the experiment, the animals were divided into 2 groups: control group, where treatment was not performed, and experimental group, where animals were treated by applying a dental film with Geranium palustre extract. The monitoring was conducted on the 3rd, 5th, 7th and 10th days of the experiment, which are key stages of healing. During these periods, blood was taken under anesthesia from the auricular vein of each experimental animal. Dynamics of prooxidant processes was detected by the level of lipid hydroperoxides and malondialdehyde, and the level of endogenic toxicity by the content of middle molecules. Statistic processing of the obtained results of investigation was performed using personal computer with installed proper software package StatSoft Statistica 10. Reliable difference was considered р<0.05. It has been shown that extract of Geranium palustre herb, as a dental film, has antioxidant properties, which are effectively reducing the intensity of lipid peroxidation processes. It should be mentioned that the content of lipid hydroperoxides and final product of lipid peroxidation processes – malondialdehyde – in experimental animals’ blood was statistically lower in all periods of investigation than in control group, where treatment was not performed. The obtained results show that higher inhibiting effect of experimental medication is marked during the final stage of lipid peroxide oxidation than during the initial stage. As the result showed, the development of endogenic toxicity was observed in both groups and the highest indices were recorded at the peak of the inflammatory process on the third day of the experiment. Dynamics of middle molecules content shows that application of Geranium palustre extract, as a dental film, decreases this index during all investigated periods and reduces intoxication level in experimental animals. Thus, application of dental film in stomatitis treatment enables to release medication directly into the damaged area, which significantly increases therapeutic effect of biologically active substances of Geranium palustre phytocomplex, and rapid absorption of the film in the oral cavity provides easy use. Considering efficacy, non-toxicity and availability of the investigated medication, the expediency of its application in dental practice for the treatment of stomatitis and other inflammatory diseases of the oral mucosa may be considered.

scholarly journals The intensity of the processes of lipid peroxidation in the blood of cows in experimental fasciolosis

2020 ◽  
Vol 22 (100) ◽  
pp. 151-155
Author(s):  
D. V. Freiuk ◽  
V. V. Stybel
Keyword(s):  
Lipid Peroxidation ◽  
Economic Damage ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Metabolic Products ◽  
End Products ◽  
Experimental Infestation ◽  
Peroxidation Processes ◽  
Experimental Group

Among the most common pathologies of parasitic etiology in ruminants, trematode diseases, namely fasciolosis, occupy a prominent place in terms of the degree of damage and economic damage caused. Activation of lipid peroxidation processes plays a significant role in the mechanisms of the development of fasciolosis in animals. The research aimed to investigate the level of lipid peroxidation products in cows' blood in experimental fasciolosis. For the test, 12 cows of 4–5 years of age, black-spotted breed, were selected, from which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selecting and keeping analog animals in groups. The cows' diet was balanced in terms of nutrients and minerals. Based on the studies, it was found that after the experimental infestation of cows of the experimental group with the causative agent of fasciolosis, an increase in the level of diene conjugates of their blood on the 15th day of the experiment by 30.3 %, and on the 25th day – by 50.4 % relative to the control group. There was also an increased level of lipid hydroperoxides in their blood, where, respectively, on the 10th and 15th day of the experiment, this figure increased by 18.3 and 32.9 % compared with the control group. On the 25th day of the experiment, the level of lipid hydroperoxides in the blood of cows of the experimental group was the highest. Compared with the control group, it increased by 46.6%, respectively. In the study of the final products of lipid peroxidation, it was found that on the 10th day of the test, the level of TBA-active products in infected cows of the experimental group increased by 9.5 %. On the 15th and 20th days of the experiment, the level of end products of LPO in the experimental group's blood of animals was higher by 20.3 and 26.0 % relative to the control group. The increase in TBA-active products' level reflects the activation of peroxide processes in animals with experimental fasciolosis. Therefore, in the clinical manifestation of fasciolosis, parasites secrete metabolic products that promote the formation of free radicals, which in turn enhance the initiation of lipid peroxidation processes.

scholarly journals Antioxidant status of piglets under nitrate-nitrite load and the action of corrective factors

2020 ◽  
Vol 3 (2) ◽  
pp. 53-59
Author(s):  
K. Y. Leskiv ◽  
B. V. Gutyj ◽  
D. F. Gufriy ◽  
V. I. Khalak ◽  
O. G. Demchuk
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Diene Conjugates ◽  
Peroxidation Processes

The article presents the results of research on the state of the antioxidant defense system of piglets under nitrate-nitrite toxicosis and the action of corrective factors (methionine, phenarone and methyphene). Nitrate-nitrite load in piglets caused inhibition of the activity of enzymes of the antioxidant defense system, that indicates a decrease in the activity of superoxide dismutase, catalase and glutathione peroxidase in the serum of testing animals. The lowest activity of antioxidant enzymes under chronic nitrate-nitrite toxicosis was on the 60th day of the experiment in the serum of piglets of the control group, where accordingly the activity of superoxide dismutase decreased by 14 %, catalase activity – by 18 %, glutathione peroxidase activity – by 12 %. Lipid peroxidation processes are activated in the blood of animals, namely the level of lipid hydroperoxides and diene conjugates increases under the conditions of experimental nitrate-nitrite load. It should be noted that the highest level of lipid peroxidation products in the serum of piglets with chronic nitrate-nitrite toxicosis was on the 60 th day of the testing, and accordingly the content of lipid hydroperoxides increased by 67 % and diene conjugates – by 33 %. Administration of methionine, phenarone and methyphene under nitrate load contributed to the activation of the antioxidant defense system compared to animals in the control group. The use of antioxidant drugs in sick piglets also contributed to the inhibition of lipid peroxidation, as indicated by a decrease in the content of lipid hydroperoxides and diene conjugates in the serum of experimental group. More pronounced antioxidant effect was shown by administrating methyphene. The mechanism of action of phenarone and methyphene is associated with the direct effect of its components on the inhibition of the absorption of metabolites that have a toxic effect on cell membranes of animals. As a result of the adsorbing action of zeolite, which is part of the drugs, in the digestive tract there is a decrease in the concentration of substances that can be substrates for lipid peroxidation, as well as removal of toxic metabolites in the blood, which are prooxidants. This process occurs by osmosis and diffusion of these substances through the capillaries of the microvilli of the small intestine and their subsequent fixation on the sorbent granules.

scholarly journals Lipid peroxidation during chronic inflammation

2018 ◽  
Vol 6 (2) ◽  
pp. 38-53
Author(s):  
Irina Sokolovskaya ◽  
Valentina Nechiporenko ◽  
Natalia Gordiyenko ◽  
Olena Pozdnyakova ◽  
Svitlana Volkova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Inflammatory Process ◽  
Positive Influence ◽  
Biologically Active ◽  
Peroxide Oxidation ◽  
Normal Functioning ◽  
Peroxidation Processes ◽  
Energy Processes ◽  
Active Substances ◽  
Physiological Systems

To keep needed level of peroxide lipid oxidation is necessary for the normal functioning of physiological systems. It is supported by the balance of anti-and prooxidant systems. The positive influence of lipid peroxidation processes in the organism is manifested by the restoration of the composition and maintenance of the properties of biological membranes, participation in energy processes, cell division, synthesis of biologically active substances. It has been established that the inflammatory process is accompanied by changes in lipid metabolism and the intensity of peroxide oxidation.

scholarly journals Peculiarities of Mineral Metabolism of Holstein Heifers’ Diet Supplemented with Copper Nanopowders

2020 ◽  
pp. 492-498
Author(s):  
Irina A. Stepanova ◽  
Anna A. Nazarova ◽  
Mikhail V. Arisov
Keyword(s):  
Treatment Group ◽  
Mineral Composition ◽  
Copper Nanoparticles ◽  
Mineral Metabolism ◽  
Biologically Active ◽  
Control Group ◽  
Experimental Animals ◽  
Animal Hair ◽  
Group A ◽  
Effect Of Copper

The current study aimed to investigate the effect of copper nanopowder on physiological and mineral metabolism indicators of Holstein cattle during the growth process of the animal. There were two experimental groups (control and treatment) and each one included seven Holstein heifers. From the first month of each heifer in the experimental group, a suspension of copper nanoparticles in a dose of 0.04 mg/kg was orally administered daily to the usual diet. The biologically active additive was administered to the animal’s diet at intervals. The weight of the animals was measured monthly, a clinical blood test was performed, the mineral composition of the blood was studied, the mineral composition of animal hair was also examined. The findings indicated a positive dynamics in the increase of body weight in the treatment group, compared to the control group. As a result of clinical blood tests, it was noted that the number of erythrocytes, the level of hemoglobin, and hematocrit increased in the experimental animals due to copper nanoparticles compared to these parameters in these animals at the beginning of the tests. Mineral analysis of blood samples in the treatment group presented an increase in the levels of copper, potassium, iron, zinc, and manganese while in animals of the control groups there was an increase in sodium, calcium, and phosphorus. According to the blood serum and hair analysis, copper in the nanodispersed state indicated antagonistic effects on boron, silicon, antimony, molybdenum of the treatment group and there was a synergist in aluminum, titanium, manganese, cobalt, iron, and potassium levels compared to the beginning of the experiment. The obtained results indicated that the addition of copper nanopowder to the diet of experimental animals increased the growth, stimulated the function of hematopoiesis, and improved the characteristics of mineral metabolism of the Holstein heifers.

scholarly journals ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ У КРОВІ КУРЧАТ–БРОЙЛЕРІВ НА ТЛІ ВАКЦИНАЦІЇ ПРОТИ ХВОРОБИ НЮКАСЛА ТА ЗА ДІЇ ВІТАМІНІВ E ТА C

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 200-204 ◽  
Author(s):  
L.V. Romanovich ◽  
B.M. Kurtyak ◽  
М.S. Romanovich ◽  
D.I. Mudrak
Keyword(s):  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Research Group ◽  
Broiler Chickens ◽  
Negative Impact ◽  
Stress Factors ◽  
Poultry Feed ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Tocopherol Acetate

The article presents research data on indices of lipid peroxidation (TBA–active products and hydroperoxides lipids) in the blood of broiler chickens on the background of vaccination and disease Nyukasla for the actions of vitamins E and C.The study was conducted on three groups of 100 broiler birds each. Control group fed normal chicken feed. The first research group in addition to poultry feed said received – tocopherol acetate in an amount of 0.1 g/kg feed, the second – ascorbic acid 0.25 g/kg feed. The third research group chickens – tocopherol acetate and ascorbic acid at these doses. For research use blood that was in the chicken after decapitation at different ages: 11–, 27–, 34– and 41–day age. The results of performance of different age groups broiler chickens compared with the value of the index poultry 11–day age for vaccination, and a control group of birds.Studies have shown that the content of intermediate and final products of lipid peroxidation in the blood plasma of broilers depends on the age and period of immunization. Feeding high amounts of vitamin E and C in the composition of feed for broiler chickens caused a reduction (p < 0.05 – 0.001) content in plasma lipid hydroperoxides and TBA–active products. PAUL likely lower rates of broiler chickens research groups suggest reducing the negative impact of stress factors on their bodies on the background of vaccination against the disease Nyukasla. These changes were more pronounced in the blood of chickens, which in addition to the basic diet receiving tocopherol acetate and ascorbic acid. 

scholarly journals Effects of histamine and sodium hypochlorite on prooxidand state in the rats erytrocytes

2020 ◽  
Vol 33 (3) ◽  
pp. 125-131
Author(s):  
Nataliya Harasym ◽  
Svitlana Mandzynets ◽  
Dmytro Sanahursky
Keyword(s):  
Lipid Peroxidation ◽  
Sodium Hypochlorite ◽  
Structural Changes ◽  
Sialic Acids ◽  
Simultaneous Action ◽  
Lipid Hydroperoxides ◽  
Rehabilitation Period ◽  
Peroxidation Processes ◽  
Structural Changes In Membranes ◽  
Positive Effect

AbstractWe studied the simultaneous influence of histamine and sodium hypochlorite (SH) on lipid peroxidation processes, as well as the level of structural changes in membranes (via the content of sialic acid) in rat erythrocytes. We established that histamine affects lipid peroxidation processes with the formation of lipid hydroperoxides, damages proteins and reduces the content of sialic acids, which leads to changes in the surface charge of red blood cells. However, the simultaneous action of histamine and low SH concentration has a positive effect in that it corrects the pro-oxidant state of erythrocytes. Hence, the content of lipid hydroperoxides, TBA-active products, carbonyl groups of proteins and sialic acids were mainly reduced after the simultaneous action of histamine and SH at all studied concentrations during the rehabilitation period.

The effect of laser phoresis of biologically active substances on microcirculation in paravertebral muscles in the radicular syndrome of the lumbosacral spine

2021 ◽  
pp. 72-76
Author(s):  
Nataliya Borisovna Korchazhkina ◽  
Ruslan Vladimirovich Kupeev
Keyword(s):  
Therapeutic Effect ◽  
Pathological Process ◽  
Main Group ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Control Group ◽  
Lumbosacral Spine ◽  
Paravertebral Muscles ◽  
Radicular Syndrome ◽  
Active Substances

In the case of radicular syndrome in patients with dorsopathy, degenerative dystrophic changes, which contribute to the creation of additional mechanisms of pain impulse and chronization of the pathological process, are diagnosed in the deep muscles. The study included 40 patients with dorsopathy of the lumbosacral spine and radicular syndrome, with the average age of 45,3±1,5years, who were divided into 2 groups comparable in age and clinical and functional characteristics: the main group of 20 patients who were treated with laser phoresis of biologically active substances and the control group of 20 patients who received standard pharmacotherapy. As a result of the study, a significantly more pronounced improvement in the state of microcirculation in the paravertebral muscles when using phytoextracts laser phoresis was proved, which was confirmed by radiothermometry and transcutaneous oximetry. The analysis of radiothermometry and oximetry parameters obtained 3 months after the end of the course showed the stability of the therapeutic effect in 96% of patients in the main group, while in the control group, 50% of patients showed a regression of the effect obtained as a result of treatment. In longer terms, the therapeutic effect in 90% of patients of the main group was maintained for 9–12 months of follow-up, while in the control group — no more than 3–4 months.

scholarly journals Studying the processes of lipid peroxidal oxidation in the organism of fresh cows under the antioxidant impact

2020 ◽  
Vol 176 ◽  
pp. 02001
Author(s):  
Lidiya Kashirina ◽  
Konstantin Ivanischev ◽  
Kirill Romanov
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Properties ◽  
The Body ◽  
Control Group ◽  
Lactation Period ◽  
Black And White ◽  
Inhibiting Effect ◽  
Peroxidation Processes ◽  
Quality Of Milk

The article contains information on the state of the processes of lipid peroxidation (LPO) in fresh cows under the influence of stress reaction caused by labor and the beginning of the lactation period. To reduce oxidative stress in the body of fresh cows, antioxidants were used in the form of preparations “E-selenium” and “Butofan”. The aim of the research was to determine the effect of vitamin-containing preparations “E-selenium” and “Butofan” on the productivity of fresh cows, the quality of milk and butter, made from it. The studies were carried out during the winter-stall period on one of the farms of Ryazan district of Ryazan region and involved 12 fresh black-and-white cows aged 4 years. The control group of animals was intact. As a result of the investigations, it was found that due to the action of the above preparations, which have antioxidant properties, the productivity parameters in the experimental groups of animals were higher compared to the control group, because they had an inhibiting effect on lipid peroxidation processes in the organism and activated the work of its own antioxidant system.

scholarly journals The quality of dairy products made from the milk of cows consuming vitamin-containing preparations

2020 ◽  
Vol 17 ◽  
pp. 00096
Author(s):  
Lydia G. Kashirina ◽  
Konstantin A. Ivanishchev ◽  
Kirill I. Romanov
Keyword(s):  
Lipid Peroxidation ◽  
Dairy Products ◽  
Antioxidant Properties ◽  
The Body ◽  
Control Group ◽  
Lactation Period ◽  
Black And White ◽  
Peroxidation Processes ◽  
Quality Of Milk

The article contains information on the state of the processes of lipid peroxidation (LPO) in fresh cows under the influence of stress reaction caused by labor and the beginning of the lactation period. To reduce oxidative stress in the body of fresh cows, antioxidants were used in the form of preparations “E-selenium” and “Butofan”. The aim of the research was to determine the effect of vitamin-containing preparations “E-selenium” and “Butofan” on the productivity of fresh cows, the quality of milk and butter, made from it. The studies were carried out during the winter-stall period on one of the farms of Ryazan district of Ryazan region and involved 12 fresh black-and-white cows aged 4 years. The control group of animals was intact. As a result of the investigations, it was found that due to the action of the above preparations, which have antioxidant properties, the productivity parameters in the experimental groups of animals were higher compared to the control group, because they had an inhibiting effect on lipid peroxidation processes in the organism and activated the work of its own antioxidant system.

scholarly journals THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN WOMEN WITH PRIMARY VARICOSE VEINS OF THE PELVIC DEPENDING ON THE STAGE OF THE DISEASE

2018 ◽  
Vol 73 (4) ◽  
pp. 229-235 ◽  
Author(s):  
Lyubov’ I. Kolesnikova, ◽  
Andrey A. Semendyaev ◽  
Dmitriy A. Stupin ◽  
Marina A. Darenskaya ◽  
[Lyudmila A. Grebenkina ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Varicose Veins ◽  
Reproductive Age ◽  
Background Information ◽  
Control Group ◽  
Maximum Increase ◽  
Diene Conjugates ◽  
Primary Varicose Veins ◽  
Peroxidation Processes

Background: Information about involvement of general nonspecific reactions, in particular lipid peroxidation processes, in the progression of varicose veins of the pelvic (VVP) in women is still too scarce. Aims: To study the intensity of processes of lipid peroxidation and the activity of components of the antioxidant system in women with primary varicose veins of the pelvic at different stages of the disease. Materials and methods: 167 women of reproductive age were examined ― 137 with VVP and 30 made up a control group. All patients with VVP were divided into 3 groups depending on stages of the disease. Spectrophotometric and fluorometric methods of investigation were used. The study was conducted during 2012−2017. Results: It was revealed that the level of primary products of lipid peroxidation, diene conjugates, increased statistically significantly according to the stage of the disease by 1.25 times (in the 1st stage), 1.51 times (in the II stage) and 1.59 times (in the III stage) values. Changes in the content of final TBA-active products showed similar changes-an increase in the mean values for all stages of the disease relative to control (in 1.24, 1.17, and 1.77 times, respectively). Activity of glutathione peroxidase increased in stage 2 of VVP (1.19 times), with the maximum increase in stage III (1.42 times); activity of glutathione-S-transferase increased 1.18 times in the II stage of the disease. The concentration of GSH in the clinical groups was characterized by lower values with respect to the control (by 1.22 times in the 1st stage, in 1.64 times in the II stage), with the maximum decrease of this parameter in the III stage of VVP (3.67 times). The level of catalase activity increased in the I stage of VVP ― by 1.18 times and decreased in the III stage ― by 1.14 times with respect to the control. The activity of SOD showed similar changes with catalase ― in the form of increased activity at the 1st stage (1.35 times higher) and decreased values for II (1.35 times lower) and III (1.65 times lower) for the stages of VVP to the values of control. Conclusions: At progression of primary VVP in women (from the initial stage to the 3rd stage of the disease), there is an increase in imbalance in the lipid peroxidation − antioxidant defense system. Moreover, if the compensatory increase in activity of antioxidant enzymes is registered at stage 1 of the disease, then the most of the antioxidant defense factors decreases as relative to control values, and the initial stages of the disease.

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