scholarly journals Analysis of adipogenesis related genes and intramuscular fat composition in Angus x Nelore cattle of different gender status

Author(s):  
Lenise Freitas Mueller da Silveira
2008 ◽  
Vol 14 (3) ◽  
pp. 251-257 ◽  
Author(s):  
V. Razmaitė ◽  
S. Kerzienė ◽  
A. Šiukščius

The aim of this study was to compare the fatty acid composition of lipids from hybrids of Lithuanian indigenous wattle pigs × wild boar (WB) intercross. A total of 39 entire and castrated males from 1/4 and 1/2 WB genotype were included into the study. The fatness of carcasses from ½ WB genotype ( p<0.01) and castrated males ( p<0.01) was higher than that from 1/4 WB genotype and entire boars. 1/2 WB hybrids had higher content of C17 : 0 ( p<0.01), C17 : 1 and C18 : 3n - 3 fatty acids ( p<0.05) and higher PUFA:SFA ratio ( p<0.05) but lower content of C16:0 fatty acid of intramuscular fat than 1/4 WB hybrids. In subcutaneous tissue 1/2 WB hybrids had higher proportion of C17:0 ( p<0.001), C17:1 ( p<0.05), C18 : 3n - 3 ( p<0.05) but lower proportion of C16 : 1 ( p<0.05). Entire boars had lower content of C16:0 ( p<0.01), C18:1 ( p<0.05), C20 :1 ( p<0.05), and the total MUFA ( p<0.05) but higher content of C18 : 2n - 6 ( p<0.05) and the total PUFA ( p<0.01), PUFA:SFA ratio ( p<0.01) than castrates. Significant Pearson's correlations of the intramuscular fat content from both genotypes with MUFA were positive, but those with PUFA and PUFA : SFA ratio were negative. High positive correlation of IMF with SFA was found only in 1/4 WB genotype. PUFA and PUFA: SFA ratio also showed negative correlation with backfat thickness in both genotypes. The correlation coefficients within entire boar and castrate groups were lower than within genetic groups.


2019 ◽  
Vol 59 (10) ◽  
pp. 1923 ◽  
Author(s):  
M. A. Latorre ◽  
A. Olivares ◽  
A. Callejo ◽  
A. I. Rey ◽  
L. Pérez-Ciria ◽  
...  

The objective of this trial was to study the impact of slaughter weight (SW) class: heavy (≥120 kg) or light (&lt;120 kg) on carcass fatness, development of main lean cuts and fat composition in barrows and gilts intended for dry-cured ham and shoulder elaboration. A total of 181 Duroc × (Landrace × Large White) pigs was used, being 94 barrows and 87 gilts. Carcasses from barrows had lower ham compactness but higher shoulder:ham weight ratio than those from gilts. The allometric growth coefficient of ham was higher in gilts whereas those for shoulder, loin and shoulder/ham were higher in barrows. An interaction sex × SW class was found for carcass fatness; the increase of fat thickness was higher in barrows than in gilts in light SW class but higher in gilts than in barrows in heavy SW class. In addition, the allometric growth coefficients for ham, loin and shoulder:ham ratio were higher in light than in heavy pigs. Percentage of rejected carcasses at slaughterhouse, due to lack of fat thickness, was higher for females than for barrows and for light than for heavy pigs. Sex and SW class had no effect on intramuscular fat content. However, more saturation was found in fat (intramuscular) from barrows and in that (subcutaneous) from light pigs. It can be concluded that carcasses from barrows are commercially more suitable than those from gilts and heavy SW might be more interesting when animals are intended for Teruel dry-cured ham and shoulder production.


2003 ◽  
Vol 77 (2) ◽  
pp. 215-224 ◽  
Author(s):  
J. Morales ◽  
M.D. Baucells ◽  
J.F. Pérez ◽  
J. Mourot ◽  
J. Gasa

AbstractWe aimed to determine whether the dietary carbohydrate source altered body fat composition and distribution in finishing lean (Landrace) and obese (Iberian) swine. To this end, twenty-four finishing castrated male pigs (12 Iberian and 12 Landrace; 108 kg live weight) were offered two diets differing in the main carbohydrates source, maize (diet M) or acorn-sorghum-maize (diet A). Diets were formulated to have the same nutrient content, except for carbohydrate fractions: diet M contained higher amount of starch (537 v. 389 g/kg) but less non-starch polysaccharides (118 v. 148 g/ kg) than diet A. At an average weight of 133 kg live weight pigs were slaughtered and their carcasses were sampled to study lipogenesis, backfat and intramuscular fat composition. Iberian pigs showed a higher voluntary food intake than Landrace pigs (3·6 v. 2·4 kg/day; P < 0·001) but no significant differences in the daily weight gain. Diet M tended to promote the highest food intake (P = 0·09). Iberian pigs showed higher (P < 0·01) lipogenic enzyme activities, backfat thickness (71·7 v. 31·9 mm) and intramuscular fat content (40 to 95 g/kg fresh muscle) than Landrace pigs, which was associated with their higher food intake. Furthermore, fat depots from Iberian pigs had higher (P < 0·001) monounsaturated fatty acids (MUFA) and lower (P < 0·05) polyunsaturated (PUFA) proportions than those from Landrace pigs. The backfat thickness of pigs given diet M tended to be higher (P = 0·07) than that of pigs given diet A, without differences in the intramuscular fat content. The higher backfat thickness found for diet M was correlated with a lower PUFA proportion in diet than for diet A (P < 0·001). We conclude that body fat content, composition and lipogenic enzyme activities are markedly influenced by the animal breed and to a lesser extent by dietary characteristics.


2019 ◽  
Vol 59 (5) ◽  
pp. 914 ◽  
Author(s):  
Pilar Gómez-Cortés ◽  
Olalla O. Galisteo ◽  
Carmen Avilés Ramírez ◽  
Francisco Peña Blanco ◽  
Miguel Angel de la Fuente ◽  
...  

The effect of concentrates based on unconventional ingredients on the intramuscular fatty acid profile of lambs was evaluated. A total of 105 lambs were assigned to one of three treatments: Control (concentrate based on cereals and soybean meal like commercial concentrates), Camelina (where 50% of crude protein from soybean meal was replaced by camelina meal), and Fibrous (concentrate based on several fibrous by-products, such as soybean hulls and wheat bran, as well as camelina meal and other unconventional ingredients). After 42 days in feedlot, the lambs were sacrificed, and the fatty acid profile of intramuscular fat was determined by gas chromatography. Total meat lipids (1.25 g/100 g of meat) were not modified by the treatments. The Camelina treatment showed minor effects on the intramuscular fat composition. In contrast, the Fibrous treatment showed the highest contents of trans-11 18:1 (vaccenic acid), cis-9 trans-11 18:2 (rumenic acid) and α-linolenic acid. Thus, the non-traditional Fibrous concentrate assayed in the present study might improve the meat quality of light lambs from a nutritional point of view.


BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Danielly Beraldo dos Santos Silva ◽  
Larissa Fernanda Simielli Fonseca ◽  
Daniel Guariz Pinheiro ◽  
Maria Malane Magalhães Muniz ◽  
Ana Fabrícia Braga Magalhães ◽  
...  

2005 ◽  
Vol 81 (2) ◽  
pp. 313-313 ◽  
Author(s):  
N. Atti ◽  
H. Rouissi ◽  
M. Mahouachi

Abstract Fifty intact male Barbarine lambs were used to assess the effects of restricted feeding and re-alimentation on carcass composition and fatty acid (FA) composition of intramuscular fat. Five lambs were slaughtered at the start of the trial; the remainder were randomly allocated to three groups. One group was offered only stubble grazing (low: L), another, also on stubble, received, indoors, an average of 80 g dry matter (DM) of soya-bean meal per day (medium: M); the third group was kept indoor and had free access to hay and 450 g of concentrate (high: H). At the end of this restriction period (70 days), five lambs per group were slaughtered. The 10 remaining animals in each group were divided into two groups receiving concentrate and hay ad libitum. The crude protein (CP) content of the concentrate was 160 and 210 g/kg DM in the two treatments, respectively. At the end of the trial all animals were slaughtered at 37·61 ± 2·05 kg live weight.In the restriction period, bone tissue was similar in terms of mass for all diet treatments. The absolute weight of muscle and fat in carcasses was higher for unrestricted sheep (H) than for restricted ones, but L and M lambs had proportionately less fat and more muscle than those given food indoors. Intramuscular fat composition was similar for all the treatments. During this period, the carcass gain of M lambs had the highest proportion of muscle and the lowest fat concentration, which is the better composition. At the end of the compensating period, all carcasses contained the same quantity of bone and muscle, but unrestricted sheep had significantly more fat than compensating ones. So, carcasses of compensating animals contained relatively less fat (240 v. 310 g/kg) and more muscle (550 v. 500 g/kg) than unrestricted ones. The carcass gain of lambs with an earlier nutritional history on L had the highest muscle and the lowest fat concentration. The intramuscular lipid composition in terms of fatty acids showed differences between treatments. Unrestricted animals contained more C14: 0 and C16: 0 and compensating animals more C18: 2 and a higher proportion of polyunsaturated fatty acids (PUFA) and PUFA: saturated FA ratio. There were no significant differences associated with CP level during the re-alimentation period


2014 ◽  
Vol 52 (01) ◽  
Author(s):  
A Kempinska ◽  
M Krawczyk ◽  
M Klak ◽  
M Blatkiewicz ◽  
F Lammert ◽  
...  

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.


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