scholarly journals The antibacterial effect of Endoseal TCS mixed with water-soluble mangostin derivatives of <i>Garcinia mangostana</i> L. ethanol extract against <i>Enterococcus faecalis</i> and <i>Staphylococcus aureus</i>

2021 ◽  
Vol 46 (1) ◽  
pp. 45-50
Author(s):  
Tae-Young Park ◽  
◽  
Yun Kyong Lim ◽  
Jin-Hee Kim ◽  
Dae Sung Lee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Water Soluble ◽  
Garcinia Mangostana ◽  
Derivatives Of

scholarly journals Physicochemical and antimicrobial assessment of Iranian Propolis gathered in Qazvin province

2020 ◽  
Vol 10 (2) ◽  
pp. 82
Author(s):  
Fatemeh Samieerad ◽  
Nematollah Gheibi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Standard Strain ◽  
Layer Chromatography

Background: Propolis is one of the useful bee colony products that have been used in traditional medicine for centuries. In this study, the physicochemical characters and their antibacterial effect of Iranian Propolis collected from Qazvin province was assessed.Methods: In this study, Thin Layer Chromatography and Vacuum Liquid Chromatography to detect different compounds of the extract have been used. In the initial evaluation of Propolis extract, it was found that the extract includes variable compounds with different polarity; so, the initial classification of extract with different polarity solvents was essential. Finally, 0.1 gr hydro alcoholic Propolis was injected to the HPLC by ultrasound. The antibacterial effect of Iranian ethanol extract Propolis was measured using a microdilution method against Pseudomonas aeruginosa: P. aeruginosa and Staphylococcus aureus: S.aureus standard strains and the minimum bactericidal and inhibitory concentration were defined.Results: Primary analysis of the ethanol extract by analytical Thin Layer Chromatography, demonstrated the presence of flavonoid and phenol in it. Minimum inhibitory concentration and Minimum Bactericidal Concentration for Staphylococcus aureus: S.aureus standard strain was 2.5mg/ml. The same procedure was done for Pseudomonas aeruginosa: P. aeruginosa standard strain and the Minimum inhibitory concentration and Minimum Bactericidal Concentration were 50mg/ml of Propolis extracts.  Conclusion: According to the results, the alcoholic extract of propolis from Qazvin province of Iran provides significant antimicrobial activity. Its powerful activity may be due to high total phenolic and flavonoid contents.Keywords: Iranian propolis, Antibacterial activity, Phenolic compounds, Flavonoid compound

scholarly journals Antibacterial activity of Ethanol Extract of Purple Passion Fruit Peel (Passiflora edulis Sims) on Staphylococcus aureus and Escherichia coli

2018 ◽  
Vol 1 (2) ◽  
pp. 29-34
Author(s):  
Sony Eka Nugraha ◽  
Suryadi Achmad ◽  
Erly Sitompul
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Water Soluble ◽  
Passion Fruit ◽  
Soluble Extract ◽  
Fruit Peel ◽  
Water Soluble Extract ◽  
Purple Passion Fruit

Abstract.North Sumatera is the central production of  purple passion fruit  in Indonesia. Passion fruit was  processing into beverage product that produced passion fruit peel waste. The passion fruit peel has potential effect as a sources of medicine which is need to be examined such as its antibacterial effect. The purpose of this study was to determine the characterization of simplicia, phytochemical screening  and antibacterial activity of ethanol extract of purple passion fruit peel against Staphylococcus aureus and Escherichia coli. The simplicia powder was examined its characteristic include water content, water soluble extract, ethanol soluble extract, total ash and acid insoluble ash assay. The extraction was process by percolation method using ethanol  96% , then tested antibacterial activity against Staphylococcus aureus and Escherichia coli using agar diffusion method with paper discs.The result showed  that  the simplicia characteristic of passion fruit peel  were water content of  8.64%, water soluble extract of  31.69%, ethanol soluble extract of 13.02%, ash total of 7.89%, and insoluble ash in acid of 0.816%. The phytochemical screening simplicia and ethanol extract showed the presence of flavonoid, glycoside, saponin, tannin and steroid/triterpenoid.The antibacterial activity test showed that the ethanol extract has an effective inhibition at the  concentration of 300 mg/ml against Staphylococcus aureus and Escherichia coli, it showed dose dependent manner.   Keywords: Passiflora edulis Sims, antibacterial, passion fruit peel, Staphylococcus aureus and Escherichia coli        

scholarly journals Uji Daya Hambat Formulasi Salep Ekstrak Etanol Umbi Hati Tanah (Angiopteris sp) terhadap Bakteri Staphylococcus aureus

2018 ◽  
Vol 5 (2) ◽  
pp. 119-125
Author(s):  
Rezqi Handayani ◽  
Nurul Qamariah
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Water Soluble ◽  
Test Material ◽  
Positive Bacterium ◽  
Plant Extraction ◽  
Ointment Formulation ◽  
Primary Test ◽  
Soluble Base ◽  
Active Substances

Hati Tanah tuber (Angiopteris sp) is a plant part that is often used by Dayak tribes in Central Kalimantan as a medicine to heal wounds. This plant is known to contain flavonoid, saponin, tannin and catechol compounds which in previous studies have proven that ethanol extract of this plant can inhibit the growth of Staphylococcus aureus bacteria. Staphylococcus aureus is a Gram-positive bacterium that causes infection in wounds. Some of these strains of bacteria are resistant to available antibiotics. In this study two bases of ointment were used, namely, the bottom of the hydrocarbon ointment and the base of the water-soluble lotion. This study aims to determine the ointment formulation which can inhibit the growth of S. aureus bacteria. Plant extraction is done by the soxhletation method. The inhibitory test in this study uses a process of planting discs that contain active substances as the primary test material. The results showed that the base ointment formulation which could inhibit the growth of Staphylococcus aureus bacteria was an ointment formulation with a water-soluble base.

scholarly journals SYNTHESIS AND STUDY OF ANTIBACTERIAL ACTIVITY OF BROMINE DERIVATIVES OF PORHYRIN POLYMERS AND THEIR ZINC COMPLEXES

2017 ◽  
Vol 60 (2) ◽  
pp. 52
Author(s):  
Nadezhda L. Pechnikova ◽  
Tatiana A. Ageeva ◽  
Oskar I. Koifman ◽  
Elena V. Alopina ◽  
Oleg Yu. Кuznetsov
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Polyvinyl Alcohol ◽  
Zinc Complexes ◽  
Water Soluble ◽  
Water Soluble Porphyrin ◽  
Derivatives Of ◽  
Porphyrin Polymers

A creation of new water-soluble porphyrin-polymers having antibacterial properties is actual today because these substances can be used in a medicine and pharmacology. Therefore, the aim of this work is to obtain a water-soluble polyvinyl alcohol-based immobilizates containing dibromo-derivatives of meso-aryl porphyrins in a side chain of the polymer and the study of their bacterial activity towards Escherichia coli ATCC 25922, Candida albicans ATCC 885-653, Staphylococcus aureus ATCC 653. The bromination of 2-formyl-5,10,15,20-tetraphenylporphyrin and its zinc (II) complexes in the phenyl rings and in the β-positions of macroheterocycle was performed. 2-Formyl-10,15-(4',4'-dibromophenyl)-5,20-diphenylporphyrin and 2-formyl-dibromo-12,13-tetraphenylporphyrin were obtained. Porphyrin metalocomplexes were prepared by the complexation reaction of porphyrin dibromo-derivatives with zinc acetate. The purification of the porphyrin metalocomplexes was carried out by the column chromatography on silica gel, using chloroform as eluent. The structure of the compounds obtained was confirmed by electronic, IR and 1H NMR spectroscopy. Water-soluble porphyrin-polymers were prepared by covalent immobilization of polyvinyl alcohol based on the synthesized porphyrins and their metalocomplexes. The immobilization was carried out by acetalization reaction in an organic medium using 20% sulfuric acid as a catalyst. The immobilizates obtained are intensely colored solid polymer products which are soluble in water and dimethylformamide. Porphyrin weight content in a series of synthesized immobilizates and their immobilization molar degree were determined by electronic absorption spectra. The highest molar degree of porphyrin immobilization on polyvinyl alcohol is achieved at a fixation of 2-formyl-dibromo-12,13-tetraphenylporphyrin and zinc-2-formyl-10,15-(4',4'-dibromo-phenyl)-5,20-diphenylporphyrine on polyvinyl alcohol. It was 0.90 and 0.86, respectively. The influence of the synthesized porphyrin-polymers on Escherichia coli АТСС 25922, Candida albicans АТСС 885-653, Staphylococcus aureus АТСС 653 was investigated by diffusional method of antibacterial activity determining. An investigation of the biological activity of water-soluble immobilizates showed that the polymers and their prepared porphyrin zinc (II) complexes have antibacterial activity against the studied cultures. Significant effect on the inhibition of given cultures have immobilizate containing zinc-2-formyl-10,15-(4',4'-dibromophenyl)-5,20-diphenylporphyrine. For citation:Pechnikova N.L., Alopina E.V., Кuznetsov O.Yu., Ageeva T.A., Koifman O.I. Synthesis and study of antibacterial activity of bromine derivatives of porhyrin polymers and their zinc complexes. Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol. 2017. V. 60. N 2. P. 52-59.

scholarly journals Uji Efek Antibakteri Ekstrak Etanol Kulit Batang Salam {Syzigium polyanthum (Wight) Walp} terhadap Staphylococcus aureus dan Escherichia coli secara Invitro

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Ikhsan Amanda Putra ◽  
Erly Erly ◽  
Machdawaty Masri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Descriptive Study ◽  
Effective Concentration ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Activity Test ◽  
Growth Of Bacteria

AbstrakTumbuhan salam (Syzygium polyanthum (Wight) Walp.) telah dikenal sejak dahulu untuk mengobati berbagai penyakit. Bagian tumbuhan yang dapat dimanfaatkan sebagai obat selain daun adalah bagian kulit batang. Beberapa penelitian yang telah dilakukan terbukti bahwa daun salam memiliki efek antibakteri. Tujuan penelitian ini adalah untuk mengetahui bagaimana efek antibakteri dari kulit batang salam. Penelitian ini bersifat deskriptif menggunakan metode difusi agar. Konsentrasi ekstrak yang digunakan adalah 25%, 50%, 75%, dan 100%. Hasil penelitian menunjukkan bahwa ekstrak etanol kulit batang salam memiliki efek antibakteri terhadap Staphylococcus aureus dengan konsentrasi 25%, 50%, 75%, dan 100% memberikan daya hambat terhadap pertumbuhan bakteri sebesar 12 mm, 13,67 mm, 12,33 mm, dan 9 mm, sedangkan pada konsentrasi yang sama untuk Escherichia coli tidak terlihat daya hambat terhadap pertumbuhan bakteri. Konsentrasi ekstrak yang paling efektif dalam menghambat S. aureus adalah konsentrasi 50%, dimana konsentrasi 75% dan 100% kurang efektif.Kata kunci: uji efek antibakteri, kulit batang salam, staphylococcus aureus, eschericia coli AbstractSalam plants (Syzygium polyanthum (Wight) Walp.) Salam plants have been known since ancient to treat various diseases. The parts of the plant that can be used as drug are bark. From the research that has been conducted has proven that Salam leaves has an antibacterial effect. The objective of this study was to determine the antibacterial effect of salam bark.This was a descriptive study by using agar diffusion method. The concentration of the extract used was 25%, 50%, 75%, and 100%. The results showed that the ethanol extract of Salam bark has antibacterial effects to Staphylococcus aureus with the concentration of 25%, 50%, 75%, and 100% gives the inhibition of the growth of bacteria on 12 mm, 13.67 mm, 12.33 mm, and the 9 mm, while at the same concentration for Escherichia coli was no bacterial inhibition area. The most effective concentration of extract in inhibiting S. aureus was concentration of 50%, while the concentration of 75% and 100% less effective.Keywords: antibacterial activity test, salam bark, staphylococcus aureus, eschericia coli

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MELINJO (Gnetum gnemon L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus dan Streptococcus mutans TAHUN 2020

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Firdaus Fahdi ◽  
Herviani Sari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Ash Content ◽  
Water Soluble ◽  
Diffusion Method ◽  
Soluble Extract ◽  
Gnetum Gnemon

The Gnetum gnemon can be used as a medicine that has the function of anti-bacterial. The chemical content inside which is anti-bacterial is flavonoids and tannins. This study aims to find out the antibacterial from the ethanol extract of melinjo leaves can inhibit the growth of Staphylococcus aureus and Streptococcus mutans with a concentration of 300 mg/ml, 400 mg/ml, and 500 mg/ml. The test of anti-bacterial activity for the growth Staphylococcus aureus and Streptococcus mutans is done by using the disc diffusion method. The results showed that melinjo leaf contains an alkaloid, flavonoid, tannin, steroid and saponin compounds. The results of the simplicia powder characteristics obtained a total ash content of 7.495%, ash content insoluble in acid 0.785%, ethanol-soluble extract 4.324%, water-soluble extract 17.690%. The results of the antibacterial activity test of the ethanol extract of melinjo leaves obtained can inhibit the growth of Staphylococcus aureus and Streptococcus mutans namely at a concentration of 300 mg/ml, 400 mg/ml, and 500 mg/ml into the medium inhibition zone category. Ethanol extract of melinjo leaves has antibacterial activity against the growth of Staphylococcus aureus and Streptococcus mutans with an average diameter of inhibition zone of 9.7 mm (medium category) against Staphylococcus aureus and 6.7 mm (medium category) against bacteria Streptococcus mutans.

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