scholarly journals Laminarin (Beta-glucan) of Brown Algae <i>Sargassum mcclurei</i>: Extraction, Antioxidant Activity, Lipoxygenase Inhibition Activity, and Physicochemistry Properties

2020 ◽  
Vol 4 (1) ◽  
pp. 31
Author(s):  
Dang Xuan Cuong
Keyword(s):  
Antioxidant Activity ◽  
Brown Algae ◽  
Inhibition Activity ◽  
Beta Glucan ◽  
Lipoxygenase Inhibition

scholarly journals ANTIOXIDANT ACTIVITY AND LIPOXYGENASE INHIBITORY ASSAY WITH TOTAL FLAVONOID CONTENT OF GARCINIA LATERIFLORA BLUME LEAVES EXTRACT

2017 ◽  
Vol 10 (17) ◽  
pp. 163
Author(s):  
Sismita Avelia ◽  
Marista Gilang Mauldina ◽  
Berna Elya
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Inhibition Activity ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Lipoxygenase Inhibition

 Objective: Garcinia lateriflora Blume has been reported to have antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method with methanol, ethyl acetate, and n-hexane leaves extract inhibitory concentration 50% (IC50) levels of 6.18, 8.03, and 156.8 μg/mL, respectively. Meanwhile, there has been no literature regarding G. lateriflora Blume’s lipoxygenase inhibition activity. The aim of this study was to determine the potential antioxidant activity and lipoxygenase inhibition activity of three leaf extracts of G. lateriflora Blume. Methods: These study test methods involved an assessment of antioxidant activity using the ferric reducing antioxidant power method, an assessment of lipoxygenase inhibition activity through the in vitro method, and a qualitative analysis of flavonoid and total flavonoid content using thin-layer chromatography and the AlCl3 colorimetric method to reveal the most active extract.Results: Based on the assessment for methanol, ethyl acetate, and n-hexane, the results showed that the effective concentration 50% levels of the antioxidant activity of G. lateriflora Blume leaves extract were 9.567, 16.555, and 50.550 μg/mL, respectively. Furthermore, the IC50 levels of the lipoxygenase inhibition activity were 0.693, 0.793, and 1.316 μg/mL, respectively. The most active extract for both of the tests was methanol extract, which has a total flavonoid content of 6.298 mg quercetin equivalents/g.Conclusions: Based on the test results, it can be concluded that G. lateriflora Blume leaves extracts exhibit antioxidant and lipoxygenase inhibition activities, with methanol extract as the most active extract, containing more flavonoid than the other two extracts.

scholarly journals Nutrient Content, Phytonutrient Composition, Alpha Amylase, Alpha Glucosidase Inhibition Activity and Antioxidant Activity of the Stoechospermum Marginatum Collected in Pre Monsoon Season

2017 ◽  
Vol 5 (3) ◽  
pp. 275
Author(s):  
Reka Palanivel ◽  
Thahira Banu Azeez ◽  
Seethalakshmi Muthaya
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Brown Algae ◽  
Phenolic Content ◽  
Monsoon Season ◽  
Nutrient Content ◽  
Alpha Amylase ◽  
Total Phenolic ◽  
Inhibition Activity ◽  
Alpha Glucosidase

The objective of this study was to investigate the nutrient content, phytonutrient composition, physicochemical properties, alpha amylase and alpha glucosidase inhibition activity and antioxidant activity of the brown algae Stoechospermum marginatum collected from Gulf of Mannar, Tamil Nadu, India in pre monsoon season (June- September, 2015). Six and eight hours of ethanol and aqueous extract of Stoechospermum marginatum were used for phytonutrient screening, alpha amylase, alpha glucosidase inhibition activity and antioxidant activity. From the results of the study it is understood that Stoechospermum marginatum contain a high amount of carbohydrate, protein, crude fiber and phytonutrients like tannin, flavonoid, saponin, alkaloid, terpenoids, steroid and total phenolic content. The physicochemical properties namely Water absorption and Swelling power were very promising. Alpha amylase and alpha glucosidase inhibition activity was recorded to be high in both aqueous and ethanol extracts of eight hour extraction than in extracts taken from six hours extraction. Antioxidant activity was detected using DPPH, FRAP, beta carotene scavenging and H2O2 assay and found to have a high radical scavenging activity. Stoechospermum marginatum possess a valuable amount of total phenolic content and other phytonutrients and physicochemical properties, it may the reason for the potential inhibition of alpha amylase, alpha glucosidase and antioxidant activity. It is concluded from the study that the brown algae may be incorporated into foods to enhance their nutritional and therapeutic value.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

scholarly journals Phenolic substances of brown algae and their antioxidant activity

2000 ◽  
Vol 36 (3) ◽  
pp. 289-291 ◽  
Author(s):  
I. D. Chkhikvishvili ◽  
Z. M. Ramazanov
Keyword(s):  
Antioxidant Activity ◽  
Brown Algae ◽  
Phenolic Substances

scholarly journals Synthesis, Neuroprotection, and Antioxidant Activity of 1,1′-Biphenylnitrones as α-Phenyl-N-tert-butylnitrone Analogues in In Vitro Ischemia Models

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1127 ◽  
Author(s):  
Beatriz Chamorro ◽  
David García-Vieira ◽  
Daniel Diez-Iriepa ◽  
Estíbaliz Garagarza ◽  
Mourad Chioua ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Human Neuroblastoma ◽  
In Vitro Ischemia ◽  
Lipoxygenase Inhibition ◽  
Antioxidant Agent

Herein, we report the neuroprotective and antioxidant activity of 1,1′-biphenyl nitrones (BPNs) 1–5 as α-phenyl-N-tert-butylnitrone analogues prepared from commercially available [1,1′-biphenyl]-4-carbaldehyde and [1,1′-biphenyl]-4,4′-dicarbaldehyde. The neuroprotection of BPNs1-5 has been measured against oligomycin A/rotenone and in an oxygen–glucose deprivation in vitro ischemia model in human neuroblastoma SH-SY5Y cells. Our results indicate that BPNs 1–5 have better neuroprotective and antioxidant properties than α-phenyl-N-tert-butylnitrone (PBN), and they are quite similar to N-acetyl-L-cysteine (NAC), which is a well-known antioxidant agent. Among the nitrones studied, homo-bis-nitrone BPHBN5, bearing two N-tert-Bu radicals at the nitrone motif, has the best neuroprotective capacity (EC50 = 13.16 ± 1.65 and 25.5 ± 3.93 μM, against the reduction in metabolic activity induced by respiratory chain blockers and oxygen–glucose deprivation in an in vitro ischemia model, respectively) as well as anti-necrotic, anti-apoptotic, and antioxidant activities (EC50 = 11.2 ± 3.94 μM), which were measured by its capacity to reduce superoxide production in human neuroblastoma SH-SY5Y cell cultures, followed by mononitrone BPMN3, with one N-Bn radical, and BPMN2, with only one N-tert-Bu substituent. The antioxidant activity of BPNs1-5 has also been analyzed for their capacity to scavenge hydroxyl free radicals (82% at 100 μM), lipoxygenase inhibition, and the inhibition of lipid peroxidation (68% at 100 μM). Results showed that although the number of nitrone groups improves the neuroprotection profile of these BPNs, the final effect is also dependent on the substitutent that is being incorporated. Thus, BPNs bearing N-tert-Bu and N-Bn groups show better neuroprotective and antioxidant properties than those substituted with Me. All these results led us to propose homo-bis-nitrone BPHBN5 as the most balanced and interesting nitrone based on its neuroprotective capacity in different neuronal models of oxidative stress and in vitro ischemia as well as its antioxidant activity.

scholarly journals SKRINING SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETILASETAT DAUN WEDUSAN (Eupatorium odoratum)

Molekul ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. 94
Author(s):  
Purwati Purwati ◽  
Undri Rastuti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Research Method ◽  
Oxidation Process ◽  
Ethyl Acetate Extract ◽  
Natural Process ◽  
Inhibition Activity ◽  
Octadecanoic Acid ◽  
Fat Quality

Oxidation process is a natural process which always occurs in fat. The process affects and decreases the fat quality. Oxidation in fat can be hampered by the addition of antioxidant. Antioxidant activity of wedusan leaf has to be studied to know the possibility of wedusan leaf as an antioxidant. Hence, the aims of the research were to determine the antioxidant activity of ethyl acetate extract of wedusan leaf using TBA method, and to compare the antioxidant activity of wedusan leaf and that of BHT. The research method consisted of sample preparation, extraction, and determination of antioxidant activity using TBA method. Wedusan leaf was extracted by maceration using n-hexane and ethyl acetate solvents. The n-hexane extract was 2.90 gram, whereas ethyl acetate extract was 13.12 gram. Based on qualitative screening on secondary metabolites, ethyl acetate extract contained flavonoid. The results from GC-MS indicated that ethyl acetate extract contained methyl heptadecanoic, methyl-13-octadecenoic, 14,16-octadecadienal, and octadecanoic acid. The order of inhibition activity of antioxidant were 0.05% (w/v) of BHT > 0.15% (w/v) of ethyl acetate extract > 0.10% (w/v) of ethyl acetate extract > 0.05% (w/v) of ethyl acetate extract.

Effect of Strains and Extraction Methods on β-Glucan Production, Antioxidant Properties, and Ftir Spectra From Mushroom Fruiting Bodies of Schizophyllum Commune FR. in Thailand

2021 ◽  
Author(s):  
Naruemon Mongkontanawat1 ◽  
Duangrutai Thumrongchote
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Chemical Structure ◽  
Antioxidant Properties ◽  
Schizophyllum Commune ◽  
Extraction Methods ◽  
Hot Water ◽  
Beta Glucan ◽  
Nutrition Value ◽  
Medicinal Properties

Schizophyllum commune Fr, a native mushroom of Thailand, has a high nutrition value and it classified as a mushroom with medicinal properties, which can neutralize the growth of many cancer cells. Thus, the aim of this research was studied effect of S. commune strain and the extraction method on the quantity and properties of &beta;-glucan. The five S. commune Fr strains consisted of Chanthaburi, 85-022, 85-023, 85-031, and 85-043, which used in this research. The &beta;-glucan extraction method compared two different extraction: hot water (M1) and hot alkali extraction (M2), with control (native-MR). It found that Chanthaburi strain has the highest in &beta;-glucan content 49.20 + 0.35 % (w/w), and high potential antioxidant activity (79.14 +0.77 DPPH % and 50.92 + 0.48 ABTS %) (p &lt; 0.05). The extraction methods had no effect on the yield of &beta;-glucan, except antioxidant properties and chemical structure of extract substance. The extract substance from M2 has significant the highest potential antioxidant activity (80.22 + 0.51 drink mushroom juice in can by using 1-day-old MR and adjust pH more than 7 can increase antioxidant properties of product.

Isolation and characterization of fucoidans from five brown algae and evaluation of their antioxidant activity

2014 ◽  
Vol 13 (5) ◽  
pp. 851-856 ◽  
Author(s):  
Guiyan Qu ◽  
Xu Liu ◽  
Dongfeng Wang ◽  
Yi Yuan ◽  
Lijun Han
Keyword(s):  
Antioxidant Activity ◽  
Brown Algae ◽  
Isolation And Characterization

Proteinase inhibition and antioxidant activity of selected forage crops

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Tibor Maliar ◽  
Jarmila Drobná ◽  
Ján Kraic ◽  
Mária Maliarová ◽  
Jana Jurovatá
Keyword(s):  
Antioxidant Activity ◽  
Trifolium Pratense ◽  
Therapeutic Potential ◽  
Antioxidant Activities ◽  
Local Population ◽  
Antioxidant Properties ◽  
Inhibition Activity ◽  
Forage Crops ◽  
Starting Point ◽  
Proteinase Inhibition

AbstractThe hypothesis of the possible therapeutic potential of selected species of forage crops is discussed. Extracts from genotypes of Anthyllis sp., Astragalus sp., Coronilla sp., Lotus sp., Medicago sp., Melilotus sp., Onobrychis sp. and Trifolium sp. were prepared and tested for proteinase inhibition and antioxidant activities. We found that Trifolium pratense accession POLKIE99-3 expressed the highest relative trypsin inhibition activity (80.0%) compared to standards. The highest thrombin inhibition activity (81.4%) was detected in the Medicago sativa old cultivar Hodoninka, whereas the highest relative urokinase inhibition activity (62.5%) was expressed by the local population Nitranka. Relatively high antioxidant properties of Trifolium sp. accessions, Trifolium pratense genetic resources SVKZAH98-40, were of interest. Results of this study confirmed that there are significant differences in proteinase inhibition and antioxidant activity among important selected agricultural crops. The present paper may also be the starting point of the research aimed for development of new functional food and nutraceuticals, and/or of the research focused on new secondary metabolites with potential as bioactive compounds.

scholarly journals Karakteristik Kandungan Fukosantin dan Aktivitas Antioksidan Ekstrak Alga Coklat (Sargassum polycystum) pada Perlakuan Konsentrasi Pelarut Aseton dan Suhu Maserasi

2020 ◽  
Vol 8 (1) ◽  
pp. 80 ◽  
Author(s):  
Ni Kadek Eka Wati ◽  
Lutfi Suhendra ◽  
Ni Made Wartini
Keyword(s):  
Antioxidant Activity ◽  
Experimental Research ◽  
Brown Algae ◽  
Solvent Concentration ◽  
Sargassum Polycystum ◽  
Tukey Test ◽  
Two Factors

Fucoxanthin has great potential to be developed as an antioxidant. The purposes of this study were to determine the effect of acetone solvent concentration and maceration temperature on the fucoxanthin content and antioxidant activity of brown algae extract (Sargassum polycystum) and determine the best acetone solvent concentration and maceration temperature on the fucoxanthin content and antioxidant activity of brown algae extract (Sargassum polycystum) . The study was the experimental research which designed by using randomized block desin (RBD) with two factors. The first factor was solvent concentration that consists of 70, 80 and 90 percent. The second factor was maceration temperature that consists of 30±2?, 40±2? and 50±2?. The data were analyzed by analysis of varian (ANOVA) and continued with the Tukey test. The results showed that the concentration of acetone solvent was influential on the content of fucoxanthin and antioxidant activity but did not affect the yield. Maceration temperature is influential on yield, fucoxanthin content and antioxidant activity. Interactions between treatments of acetone solvent concentration and maceration temperature greatly affect the content of fucoxanthin and antioxidant activity but do not affect the yield. The treatment of acetone 80 percent solvent concentration and maceration temperature 30±2? is the best treatment to produce brown algae extract (Sargassum polycystum) with a yield of 3.24 ± 0.45 percent, fucoxanthin content 1.03 ± 0.01 g fukosantin/g and antioxidant activity 99 ± 8.49 ppm and show activity powerful antioxidant. Keywords: Sargassum polycystum, acetone, maceration, fucoxanthin, antioxidant.

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