Extracellular Biosynthesis, Characterization and Cytotoxic Effect of Silver Nanoparticles by Streptomyces coelicoflavus KS-3

2018 ◽  
Vol 18 (12) ◽  
pp. 8133-8141 ◽  
Author(s):  
Liang Ma ◽  
Jianxin Liu ◽  
Wei Su ◽  
Xiaoxi Zeng ◽  
Xueying Liu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cytotoxic Effect ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Silver Ions ◽  
Dependent Manner ◽  
Face Centered Cubic ◽  
16S Rrna Analysis ◽  
X Ray ◽  
Extracellular Biosynthesis

The present work aimed to investigate extracellular biosynthesis of silver nanoparticles (AgNPs) mediated by an actinomycete strain and their cytotoxic effects compared with silver ions. The selected strain was identified as Streptomyces coelicoflavus KS-3 by phenotypical characteristics and 16S rRNA analysis. The formation of biosynthesized AgNPs was proved by an absorption peak observed at 437 nm. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analyses revealed that the prepared AgNPs were spherical or approximately spherical followed by a small amount of truncated triangular, quadrangular and hexagonal with the particle diameters ranging from 2.33 to 91.3 nm. X-ray diffraction (XRD) pattern confirmed that the AgNPs presented a face-centered cubic (FCC) structure of crystalline silver. Energy dispersive of X-ray (EDX) spectrum and Fourier transform infrared spectroscopy (FTIR) analyses verified the existence of biomolecules, such as proteins, that participated in the formation and stabilization of AgNPs. Furthermore, the comparative study on cytotoxic effect of AgNPs indicated that the AgNPs exhibited higher biocompatibility towards human bronchial epithelial (HBE) cells than silver ions and exerted potent cytotoxic effect in a dose-dependent manner against human lung squamous cell carcinoma cells (HTB-182) and human lung adenocarcinoma cells (A549) with the concentrations ranging from 1 to 50 μg/mL.

Utilization of Aquatic Weed for Environmental and Rapid Synthesis of Silver Nanoparticles

2013 ◽  
Vol 662 ◽  
pp. 80-83 ◽  
Author(s):  
Mana Intarasawang ◽  
Kheamrutai Thamaphat
Keyword(s):  
Silver Nanoparticles ◽  
Water Hyacinth ◽  
Cell Structure ◽  
Silver Ions ◽  
Spherical Particles ◽  
Aquatic Weed ◽  
Face Centered Cubic ◽  
X Ray ◽  
Light Yellow ◽  
Face Centered

In the present study, we demonstrated an effective cost and environmentally benign method to synthesize the silver nanoparticles (AgNPs) using aquatic weed namely water hyacinth as a reducing agent. Ultraviolet irradiation was also combined in the process to enhance the reaction rate. The aqueous silver ions mixed with water hyacinth leaf extract under UV irradiation were reduced to AgNPs. The mixture color was changed from light yellow to yellowish-brown color within 5 min due to excitation of surface plasmon vibrations in AgNPs. The formation of AgNPs was confirmed by UV-visible spectroscopy, X-ray diffraction (XRD) spectrometry, energy dispersive X-ray (EDX) analysis and transmission electron microscopy (TEM). The AgNPs were found to be polycrystalline in nature and spherical particles with mean diameter of 12.54 ± 0.19 nm. The XRD pattern peaked at different diffraction angles corresponding to the (111), (200), (220), and (311) planes indicated that AgNPs have face-centered cubic (fcc) unit cell structure. This proposed method revealed that unwanted plant can utilize in nano-manufacturing as well.

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1777 ◽  
Author(s):  
Md. Mahiuddin ◽  
Prianka Saha ◽  
Bungo Ochiai
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ftir Spectroscopy ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

Pretreated Lignocellulosic Waste Mediated Biosynthesis of Silver Nanoparticles Under Room Temperature

2016 ◽  
Vol 15 (05n06) ◽  
pp. 1660001 ◽  
Author(s):  
V. P. Manjamadha ◽  
Karuppan Muthukumar
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Room Temperature ◽  
Nitrate Solution ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
Morphological Characterization ◽  
Alternative Source ◽  
X Ray ◽  
Antibacterial Performance

The current work elucidates the utilization of biowaste as a valuable reducing agent for the synthesis of silver nanoparticles. In this study, the wastewater generated during the alkaline pretreatment of lignocellulosic wastes (APLW) was used as a bioreductant to reduce silver nitrate under room temperature. Synthesis of stable silver nanoparticles (AgNPs) was achieved rapidly on addition of APLW into the silver nitrate solution (1[Formula: see text]mM). The morphological characterization of AgNPs was performed using field emission scanning electron microscopy (FESEM). The micrograph clearly depicted the presence of spherical AgNPs. The presence of elemental silver along with biomoilties was determined using energy dispersive X-ray spectroscopy (EDAX) analysis. The X-ray diffraction (XRD) study proved the crystalline form of stable AgNPs. The AgNPs exhibited excellent antibacterial performance against Gram negative organism. The immediate bioreduction of silver ions using APLW was well illustrated in the present study. Thus, APLW serve as an alternative source for reducing agents instead of utilizing valuable medicinal plants for nanoparticles synthesis.

scholarly journals Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

2019 ◽  
Vol 31 (12) ◽  
pp. 2804-2810
Author(s):  
Anti Kolonial Prodjosantoso ◽  
Oktanio Sigit Prawoko ◽  
Maximus Pranjoto Utomo ◽  
Lis Permana Sari
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Silver Ions ◽  
Reduction Reaction ◽  
Face Centered Cubic ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Face Centered

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

scholarly journals Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sudip Some ◽  
Onur Bulut ◽  
Kinkar Biswas ◽  
Anoop Kumar ◽  
Anupam Roy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Silver Ions ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Beneficial Effects ◽  
Selected Area Electron Diffraction ◽  
Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

scholarly journals Silver Nanoparticles Decrease the Viability of Cryptosporidium parvum Oocysts

2015 ◽  
Vol 82 (2) ◽  
pp. 431-437 ◽  
Author(s):  
Pamela Cameron ◽  
Birgit K. Gaiser ◽  
Bidha Bhandari ◽  
Paul M. Bartley ◽  
Frank Katzer ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cryptosporidium Parvum ◽  
Silver Ions ◽  
Protozoan Parasite ◽  
Dependent Manner ◽  
Oocyst Wall ◽  
Content Type ◽  
Chlorine Disinfection ◽  
High Concentrations ◽  
Dose Dependent

ABSTRACTOocysts of the waterborne protozoan parasiteCryptosporidium parvumare highly resistant to chlorine disinfection. We show here that both silver nanoparticles (AgNPs) and silver ions significantly decrease oocyst viability, in a dose-dependent manner, between concentrations of 0.005 and 500 μg/ml, as assessed by an excystation assay and the shell/sporozoite ratio. For percent excystation, the results are statistically significant for 500 μg/ml of AgNPs, with reductions from 83% for the control to 33% with AgNPs. For Ag ions, the results were statistically significant at 500 and 5,000 μg/ml, but the percent excystation values were reduced only to 66 and 62%, respectively, from 86% for the control. The sporozoite/shell ratio was affected to a greater extent following AgNP exposure, presumably because sporozoites are destroyed by interaction with NPs. We also demonstrated via hyperspectral imaging that there is a dual mode of interaction, with Ag ions entering the oocyst and destroying the sporozoites while AgNPs interact with the cell wall and, at high concentrations, are able to fully break the oocyst wall.

scholarly journals Degradation of Methylene Blue Using Biologically Synthesized Silver Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
M. Baburaja ◽  
S. Rajeshkumar ◽  
G. Gnanajobitha ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Reaction Medium ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Nowadays plant mediated synthesis of nanoparticles has great interest and achievement due to its eco-benign and low time consuming properties. In this study silver nanoparticles were successfully synthesized by usingMorinda tinctorialeaf extract under different pH. The aqueous leaf extract was added to silver nitrate solution; the color of the reaction medium was changed from pale yellow to brown and that indicates reduction of silver ions to silver nanoparticles. Thus synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer. Dispersity and morphology was characterized by scanning electron microscope (SEM); crystalline nature and purity of synthesized silver nanoparticles were revealed by X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). FTIR spectrum was examined to identify the effective functional molecules responsible for the reduction and stabilization of silver nanoparticles synthesized by leaf extract. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Green synthesized silver nanoparticles were effectively degrading the dye nearly 95% at 72 h of exposure time.

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