Rapid Detection of Fungal Keratitis with DNA-Stabilizing FTA Filter Paper

Inexpensive, reusable electrochemical chips were configured as immunosensors by using a filter paper disk equipped with antibodies. Rapid detection of cancer biomarker protein prostate specific antigen (PSA) in serum was achieved with 6 pg mL−1 detection in ∼15 min.

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SERS Platform Based on Bimetallic Au-Ag Nanowires-Decorated Filter Paper for Rapid Detection of miR-196ain Lung Cancer Patients Serum

Journal of Chemistry ◽

10.1155/2020/5073451 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Ji Xia ◽

Yifan Liu ◽

Menglin Ran ◽

Dan Lu ◽

Xiaowei Cao ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Patients ◽

Filter Paper ◽

Rapid Detection ◽

Lung Cancers ◽

Lung Cancer Patients ◽

Single Base Mismatch ◽

Liver Cancers ◽

Surface Enhanced Raman ◽

Potential Applications

Detecting microRNA (miRNA) biomarkers expression is of great significance for the diagnosis and treatment of lung cancer. Surface-enhanced Raman scattering (SERS) has achieved microRNA sensing for the diagnosis of primary liver cancers. In this work, we developed a SERS technology for the rapid detection of lung cancers-related miRNA (miR-196a) using bimetallic Au-Ag nanowire (AgNW@AuNPs) substrates coupled with the target hairpin DNA. The finite-difference time-domain simulation proved that a large number of “hot spots” were generated between the AgNW and AuNPs, which resulted in a huge enhancement of the signal of Raman reporters. Filter paper treated by hexadecenyl succinic anhydride hydrophobic and modified with AgNWs@AuNPs was used as capturing substrate. The detection limits of miR-196a in PBS and serum were as low as 96.58 aM and 130 aM, respectively. Studies on nonspecific sequence and single-base mismatch of miRNA demonstrated that SERS-based platform was highly selective, excellent uniform, and reproducible. Finally, the platform was used to show that the miR-196a expression in the serum of lung cancer patients was much higher than that in healthy people. The detection results indicated that the SERS platform had potential applications in cancer diagnosis and might be a viable alternative to the conventional miRNA detection method, the real-time polymerase chain reaction (RT-PCR) technology.

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Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities ofFusariumandAspergillusIsolates from Corneal Scrapings

BioMed Research International ◽

10.1155/2019/6395840 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 17

Author(s):

Palanisamy Manikandan ◽

Ahmed Abdel-hadi ◽

Yendrembam Randhir Babu Singh ◽

Rajaraman Revathi ◽

Raghavan Anita ◽

...

Keyword(s):

Rapid Detection ◽

Corneal Ulcer ◽

Fungal Keratitis ◽

Mycotic Keratitis ◽

Common Cause ◽

Epidemiological Trend ◽

Male Preponderance ◽

The Common ◽

Ocular Morbidity ◽

Culture Positive

Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly,FusariumandAspergillusare reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis patients, of which 411 (82.2%) were culture positive for bacteria, fungi, and parasites. Among fungal aetiologies,Fusarium(216, 52.5% of 411) andAspergillus(68, 16.5% of 411) were predominantly determined. While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active, respectively, againstFusariumspp. andAspergillusspp. Additionally, 94.1% of culture provenFusariumkeratitis and, respectively, 100% and 63.6% ofA. flavusandA. fumigatuswere confirmed by multiplex PCR. The sensitivity of the PCR employed in the present study was noted to be 10 fg/μl, 1 pg/μl, and 300 pg/μl of DNA, respectively, forFusarium,A. flavus,andA. fumigatus.Alarming fact was thatFusariumandAspergillusregionally remained to be the common cause of mycotic keratitis and theFusariumisolates had a higher antifungal resistance thanAspergillusstrains against most of the test drugs.

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Simple and rapid detection of the porcine reproductive and respiratory syndrome virus from pig whole blood using filter paper

Journal of Virological Methods ◽

10.1016/j.jviromet.2006.11.030 ◽

2007 ◽

Vol 141 (1) ◽

pp. 102-106 ◽

Cited By ~ 21

Author(s):

Ryo Inoue ◽

Takamitsu Tsukahara ◽

Chinatsu Sunaba ◽

Mitsugi Itoh ◽

Kazunari Ushida

Keyword(s):

Filter Paper ◽

Whole Blood ◽

Rapid Detection ◽

Respiratory Syndrome Virus

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Rapid Detection of Organic Phosphorus Poisons

Clinical Chemistry ◽

10.1093/clinchem/14.4.371 ◽

1968 ◽

Vol 14 (4) ◽

pp. 371-373 ◽

Cited By ~ 3

Author(s):

J Fischl ◽

N Pinto ◽

C Gordon

Keyword(s):

Filter Paper ◽

Rapid Detection ◽

Organic Phosphorus ◽

Wetting Agent ◽

Phenol Red ◽

Air Samples ◽

Substrate Solution ◽

Yellow Color ◽

Bacterial Decomposition

Abstract Strips of filter paper are impregnated with a buffered acetylcholine substrate solution, containing phenol red as an indicator, and 1,2-propyleneglycol serving as an inhibitor of bacterial decomposition and as a wetting agent. The test is suitable for the detection of the poison in vivo, as well as in suspected materials such as food, clothing, water, soil, and air samples. The test is based on the inhibition of acetylcholinesterase by the organophosphoric group of the substances. In positive tests the indicator paper turns pink-to-violet and in negative tests it turns a yellow color.

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Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072800 ◽

1973 ◽

Vol 31 ◽

pp. 472-473

Author(s):

Linda M. Sicko ◽

Thomas E. Jensen

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Critical Point ◽

Filter Paper ◽

Freeze Drying ◽

Cell Surfaces ◽

Air Drying ◽

Popular Method ◽

Critical Point Drying ◽

Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

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Search mode for rapid detection of virus particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085733 ◽

1991 ◽

Vol 49 ◽

pp. 286-287

Author(s):

O. E. Bradfute

Keyword(s):

Electron Microscopy ◽

Rapid Detection ◽

Plant Virus ◽

Low Dose ◽

Search Mode ◽

Virus Diseases ◽

Virus Particles ◽

Focus Image ◽

Time Required ◽

Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

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Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142815 ◽

1986 ◽

Vol 44 ◽

pp. 238-239

Author(s):

C.D. Humphrey ◽

T.L. Cromeans ◽

E.H. Cook ◽

D.W. Bradley

Keyword(s):

Electron Microscopy ◽

Liquid Phase ◽

Cell Cultures ◽

Rapid Detection ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Solid Phase ◽

Immune Electron Microscopy ◽

Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

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