scholarly journals A Comparison of the Cyclic Variation in Serum Levels of CA125 Across the Menstrual Cycle Using Two Commercial Assays

2011 ◽  
Vol 14 (3) ◽  
pp. 250-256 ◽  
Author(s):  
Monica R. McLemore ◽  
Bradley E. Aouizerat ◽  
Kathryn A. Lee ◽  
Lee-may Chen ◽  
Bruce Cooper ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Premenopausal Women ◽  
Serum Levels ◽  
Population Based ◽  
Reference Ranges ◽  
Healthy Women ◽  
Serum Ca125 ◽  
Caucasian Women ◽  
Clinically Significant ◽  
Good Concordance

Background: Clinicians use CA125, a tumor-associated antigen, primarily to monitor epithelial ovarian cancer. However, CA125 lacks the sensitivity and specificity necessary for population-based screening in healthy women. The purpose of this study was to determine if serum concentrations of CA125 differed across the three phases of the menstrual cycle in healthy, premenopausal women using two commercially available assays. Methods: Healthy, Caucasian women between the ages of 18 and 39 were enrolled using strict criteria to exclude factors known to contribute to CA125 fluctuations. Menstrual cycle regularity was determined using calendars maintained by participants for 3 months. After cycle regularity was established, blood was drawn at three time points for CA125 determination using two commercial assays (i.e., Siemens and Panomics). Results: Regardless of the assay used, CA125 values were highest during menses. The CA125 values decreased 0.2 U/ml per day from menses to the end of the same cycle, which resulted in a net decrease of 5.8 U/ml across the cycle. Conclusions: The two commercial assays for CA125 determination demonstrated good concordance in terms of reference ranges regardless of epitope differences. While CA125 levels changed over the course of the menstrual cycle, these changes may not be clinically significant in healthy women. This study is the first to control for factors known to contribute to CA125 elevations; to quantify a decrease in CA125 levels across the menstrual cycle; and to confirm concordance in the relative decreases in serum CA125 levels across the menstrual cycle between two frequently used commercial assays.

scholarly journals Differences in the Molecular Species of CA125 Across the Phases of the Menstrual Cycle

2015 ◽  
Vol 18 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Monica R. McLemore ◽  
Christine Miaskowski ◽  
Kathryn Lee ◽  
Lee-may Chen ◽  
Bradley E. Aouizerat
Keyword(s):  
Menstrual Cycle ◽  
Western Blot ◽  
Molecular Species ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Clinical Importance ◽  
Future Research ◽  
Healthy Women ◽  
Serum Ca125 ◽  
Caucasian Women

Background:CA125, a tumor-associated antigen, is primarily used to monitor epithelial ovarian cancer. There is evidence that different species of CA125 exist; however, it is not known if any of these species are present in healthy women during the menstrual cycle and if they are associated with serum concentrations of CA125. The purpose of this study was to determine if the molecular species of CA125 differ across the three phases of the menstrual cycle in healthy women.Methods:Healthy, Caucasian women between the ages of 18 and 39 were enrolled using strict criteria to exclude factors known to contribute to CA125 fluctuations. Menstrual cycle regularity was determined using calendars maintained by participants for 3 months. After cycle regularity was established, blood was drawn at three time points for Western blot analysis.Results:Western blot analysis yielded 17 distinct profiles (i.e., patterns of species) of CA125, with 80% of the sample exhibiting 5 common profiles. No differences in demographic characteristics and serum CA125 values were found among the various CA125 profiles.Conclusions:Different molecular species of CA125 exist in healthy women with regular menstrual cycles. These data provide evidence that CA125 is not a homogeneous molecular species. Future research should evaluate the molecular composition and the clinical importance of these species.

scholarly journals Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS

2019 ◽  
Vol 105 (3) ◽  
pp. 754-768 ◽  
Author(s):  
Hanne Frederiksen ◽  
Trine Holm Johannsen ◽  
Stine Ehlern Andersen ◽  
Jakob Albrethsen ◽  
Selma Kløve Landersoe ◽  
...  
Keyword(s):  
Method Development ◽  
Premenopausal Women ◽  
Population Based ◽  
Reference Intervals ◽  
Future Research ◽  
Specific Reference ◽  
Reference Ranges ◽  
Pubertal Stage ◽  
Cross Sectional ◽  
Liquid Chromatography Tandem Mass

Abstract Context The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations. Objective To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3). Design LC-MS/MS method development and construction of estrogen reference ranges. Settings Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas. Participants Healthy participants aged 3 months to 61 years (n = 1838). Results An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from <LOD to 100 pmol/L during mini-puberty, whereas it was ≤20 pmol/L during childhood. E1 and E2 increased with age and pubertal breast stage and varied during the menstrual cycle; E1 was lower than E2 in girls and premenopausal women, and higher than E2 in postmenopausal women. In boys, E1 and E2 increased with age and pubertal stage, whereas little changes with age were observed in men. High E3 concentrations were confirmed in pregnant women. Conclusion Reference ranges of simultaneous quantification of E1 and E2 by this novel specific and highly sensitive LC-MS/MS method provide an invaluable tool in clinical practice and in future research studies.

scholarly journals Reference values for serum levels of vitamin B12and folic acid in a population-based sample of adults between 35 and 80 years of age

2002 ◽  
Vol 5 (3) ◽  
pp. 505-511 ◽  
Author(s):  
Åke Wahlin ◽  
Lars Bäckman ◽  
Johan Hultdin ◽  
Rolf Adolfsson ◽  
Lars-Göran Nilsson
Keyword(s):  
Folic Acid ◽  
Sex Education ◽  
Reference Data ◽  
Serum Levels ◽  
Population Based ◽  
Vitamin B ◽  
Reference Ranges ◽  
Mean Cell Volume ◽  
Vitamin Status ◽  
Age Related

AbstractObjectives:To examine folic acid and vitamin B12status in a group of 1000 persons sampled from the community of Umeå, Sweden, and aged 35, 40, 45, 50, 55, 60, 65, 70, 75 or 80 years. Reference data for folate and age-stratified reference data for vitamin B12are presented, together with an examination of potential confounders.Measurements:All subjects participated in extensive health examinations and interviews, and laboratory blood testing was performed.Results:A series of exclusion criteria were applied, and data from 961 subjects were analysed. Vitamin B12levels were found to decrease with increasing age, whereas folate levels remained constant across the age span studied. None of the vitamins was found to vary with sex, education, smoking or alcohol consumption, body mass index, prescription-free vitamin supplements, level of haemoglobin, or mean cell volume of erythrocytes. Further, none of these factors was associated with the age-related decrease of vitamin B12level.Conclusions:The offered reference ranges should be used only in order to rule out deficiency. For B12levels, the age of the subject should be considered such that, for elderly people in particular, values above the medians should be considered as indicative of normal vitamin status.

scholarly journals Interocular Symmetry Analysis of Corneal Elevation Using the Fellow Eye as the Reference Surface and Machine Learning

Healthcare ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1738
Author(s):  
Shiva Mehravaran ◽  
Iman Dehzangi ◽  
Md Mahmudur Rahman
Keyword(s):  
Machine Learning ◽  
Mirror Symmetry ◽  
Single Point ◽  
Population Based ◽  
Reference Ranges ◽  
Reference Surface ◽  
Fellow Eye ◽  
Elevation Data ◽  
Clinically Significant ◽  
Middle Aged Adults

Unilateral corneal indices and topography maps are routinely used in practice, however, although there is consensus that fellow-eye asymmetry can be clinically significant, symmetry studies are limited to local curvature and single-point thickness or elevation measures. To improve our current practices, there is a need to devise algorithms for generating symmetry colormaps, study and categorize their patterns, and develop reference ranges for new global discriminative indices for identifying abnormal corneas. In this work, we test the feasibility of using the fellow eye as the reference surface for studying elevation symmetry throughout the entire corneal surface using 9230 raw Pentacam files from a population-based cohort of 4613 middle-aged adults. The 140 × 140 matrix of anterior elevation data in these files were handled with Python to subtract matrices, create color-coded maps, and engineer features for machine learning. The most common pattern was a monochrome circle (“flat”) denoting excellent mirror symmetry. Other discernible patterns were named “tilt”, “cone”, and “four-leaf”. Clustering was done with different combinations of features and various algorithms using Waikato Environment for Knowledge Analysis (WEKA). Our proposed approach can identify cases that may appear normal in each eye individually but need further testing. This work will be enhanced by including data of posterior elevation, thickness, and common diagnostic indices.

scholarly journals Neuroendocrine disorders in women with epilepsy depending on anticonvulsants intake

2013 ◽  
Vol 94 (1) ◽  
pp. 63-67
Author(s):  
S N Mekhtieva
Keyword(s):  
Menstrual Cycle ◽  
Serum Level ◽  
Serum Levels ◽  
Reproductive Age ◽  
Endocrine Function ◽  
Control Group ◽  
Reference Ranges ◽  
Hormone Release ◽  
Female Patients ◽  
Patients With Epilepsy

Aim. To assess the influence of epilepsy and anticonvulsants on central control of hormone release and ovarian function in women of reproductive age. Methods. The reproductive and endocrine functions were assessed in 82 female patients with epilepsy, of whom 37 did not receive anticonvulsants (first group), 45 patients were on anticonvulsant treatment (second group). 17 healthy females (control group) were also assessed. Serum levels of estradiol, progesterone, prolactin, luteinizing hormone, follicle-stimulating hormone and dehydroepiandrosterone were estimated during follicular (n=48) and luteal (n=34) stages of menstrual cycle. Results. Estradiol serum level was below normal ranges, and prolactin serum level - above normal limits at follicular stage of menstrual cycle in female patients not treated with anticonvulsants. In luteal phase serum levels of estradiol, progesterone, dehydroepiandrosterone exceeded the reference ranges, and testosterone levels were lower than normal in both patients treated and not treated with anticonvulsants. Comparison of two groups showed that estradiol serum level was lower in untreated patients in both stages of menstrual cycle compared to patients treated with anticonvulsants. There was a marked difference in endocrine function at all levels of reproductive neuroendocrine regulation in untreated female patients compared to female patients treated with anticonvulsants with major transformations in female patients with epilepsy not treated with anticonvulsants. Conclision. Epilepsy and treatment with anticonvulsants can influence the central regulation of hormone release in females.

Androgens During the Reproductive Years: What Is Normal for Women?

2019 ◽  
Vol 104 (11) ◽  
pp. 5382-5392 ◽  
Author(s):  
Marina A Skiba ◽  
Robin J Bell ◽  
Rakibul M Islam ◽  
David J Handelsman ◽  
Reena Desai ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Premenopausal Women ◽  
Cross Sectional Study ◽  
Normal Weight ◽  
Specific Reference ◽  
Reference Ranges ◽  
Cross Sectional ◽  
Early Follicular Phase ◽  
Liquid Chromatography Tandem Mass ◽  
Androgen Levels

Abstract Objective Whether serum androgen levels can identify women with “androgen insufficiency” or “androgen excess” is unresolved; thus, what constitutes “normal” remains uncertain. We sought to determine whether androgens, including 11-oxygenated C19 steroids, vary with age, menstrual cycle, or body mass index (BMI), during the reproductive years. Design and Setting Cross-sectional study recruited from eastern Australian states. Participants A total of 588 women, aged 18 to 39 years, who were not pregnant, lactating, or using systemic hormone therapy, with regular menstrual cycles and no previous diagnosis of polycystic ovarian syndrome. Main Outcome Measures Sex steroids measured using liquid chromatography-tandem mass spectrometry. Results Testosterone and androstenedione concentrations were significantly higher during the menstrual cycle mid- and luteal phases than in the early follicular phase, with median values across the cycle of 0.34 nmol/L (range, 0.04 to 1.01) and 1.97 nmol/L (range, 0.53 to 7.89), respectively. No cyclical variations were found in dehydroepiandrosterone (DHEA; 4.91 nmol/L; range, 0.08 to 23.51), 11-ketoandrostenedione (11KA; 7.99 nmol/L; range, 0.07 to 31.67), or 11-ketotestosterone (11KT; 1.27 nmol/L; range, 0.03 to 7.61). Overweight women had lower median testosterone (P < 0.05), DHEA (P < 0.05), and 11KA (P < 0.01) levels than normal-weight women. All C19 steroids were significantly lower (P < 0.01) in those aged 35 to 39 years than in those aged 18 to 25 years. The median 11KA/androstenedione (4.3:1) and 11KT/testosterone (3.9:1) ratios did not change with age, after adjustment for BMI and cycle stage. Conclusions We have demonstrated that 11KA and 11KT are stable across the menstrual cycle and make major quantitative contributions to the circulating androgen pool. All C19 androgens declined with age before menopause; hence, age-specific reference ranges are required for the interpretation of androgen levels in premenopausal women.

The blunting of the thyrotropin response to repeated thyrotropin-releasing hormone administration is reduced by dopaminergic blockade both at normal and elevated serum levels of thyroid hormones

1990 ◽  
Vol 123 (5) ◽  
pp. 519-525 ◽  
Author(s):  
Eva Marie T. Erfurth ◽  
Robyn Attewell ◽  
L. Pavo Hedner
Keyword(s):  
Menstrual Cycle ◽  
Elevated Serum ◽  
Premenopausal Women ◽  
Serum Levels ◽  
Follicular Phase ◽  
Serum Estradiol ◽  
Trh Test ◽  
Serum Estradiol Level ◽  
Hormone Administration ◽  
Estradiol Levels

Abstract. Fifteen premenopausal women were investigated in the follicular phase of the menstrual cycle with two TRH tests within an interval of 48 to 96 h. Ninety min before each TRH test either 10 mg metoclopramide or saline was injected iv in randomized order. The same procedure was repeated in the following menstrual cycle after pretreatment with T4 (0.5 mg daily for 6-14 days). At least 2 months later the same procedure was repeated with T3 pretreatment (60-120 μg for 6-8 days) in 9 of them. A multiple regression analysis was used in modelling the relationships between TSH release and serum free T3, T4 and estradiol levels, adjusting for the presence of metoclopramide and the order of the test. No correlation was found between the TSH response to TRH and the serum estradiol level. The TSH response to the second TRH test was approximately half the first one, both in the control situation and after treatment with T4 or T3. The blunting of the TSH response to the second TRH administration was significantly reduced by metoclopramide, both at normal and elevated thyroid hormone levels, suggesting that a dopaminergic mechanism takes part in the blunting.

scholarly journals Association between menstrual cycle irregularity and tinnitus: a nationwide population-based study

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jin-Na Yu ◽  
Ga Eun Nam ◽  
Kyungdo Han ◽  
Ji-su Kim ◽  
Yang-Hyun Kim ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Premenopausal Women ◽  
Population Based ◽  
Multivariable Logistic Regression Analysis ◽  
Related Factor ◽  
Menstrual Irregularity ◽  
Childbearing Age ◽  
Cross Sectional ◽  
Menstrual Cycles ◽  
Menstrual Cycle Irregularity

Abstract This population-based cross-sectional study investigated the association between menstrual cycle irregularity and tinnitus in premenopausal Korean women. We used data from the 5th Korea National Health and Nutrition Examination Survey (2010–2012). A total of 4633 premenopausal women were included. Hierarchical multivariable logistic regression analysis was performed. Individuals with tinnitus accounted for 21.6%. Women with tinnitus or menstrual irregularity had significantly higher rates of stress, depressive mood, and suicidal ideation than those without. The proportion of individuals with irregular menstrual cycles with duration of longer than 3 months increased as the severity of tinnitus increased (P = 0.01). After adjusting for confounding variables, the odds of tinnitus increased in individuals with irregular menstrual cycles compared to those with regular menstrual cycles. The odds ratios (ORs) of tinnitus tended to increase as the duration of menstrual irregularity became longer (1.37, 95% confidence interval: 1.06–1.78 for duration of up to 3 months; 1.71, 1.03–2.85 for duration of longer than 3 months, P for trend = 0.002). Our study found a positive association between menstrual cycle irregularity and tinnitus. Menstrual cycle irregularity may be a related factor of tinnitus in women with childbearing age.

scholarly journals Determinants and Reference Ranges of Serum Immunoglobulins in Middle-Aged and Elderly Individuals: a Population-Based Study

2021 ◽  
Author(s):  
Samer Raza Khan ◽  
Layal Chaker ◽  
Mohammad Arfan Ikram ◽  
Robin Patrick Peeters ◽  
Petrus Martinus van Hagen ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Heavy Drinking ◽  
Serum Levels ◽  
Population Based ◽  
Community Dwelling ◽  
Specific Reference ◽  
Reference Ranges ◽  
Middle Aged ◽  
Higher Alcohol ◽  
Elderly Individuals

Abstract Purpose In clinical practice, currently one reference range for serum immunoglobulin (Ig) A, G, and M is applied to all adults, although various factors may influence Ig serum levels. Population-based data on determinants of IgA, IgG, and IgM and recommendations for subgroup specific reference ranges are lacking. We aimed to provide an overview of determinants of IgA, IgG, and IgM in community-dwelling middle-aged and elderly individuals and explore determinants that influence Ig reference ranges. Methods Within the Rotterdam Study, we performed linear regression analyses for the association of demographic, lifestyle, and cardiovascular factors with serum IgA, IgG, and IgM. We furthermore calculated Ig reference ranges (based on percentiles), both overall and within relevant subgroups. Results We included 8768 participants (median age 62 years). IgA and IgG increased non-linearly with higher age (P < .0001 for both). Women had lower IgA (beta: − 0.24; 95% confidence interval [95% CI]: − 0.29; − 0.20) and IgG (beta: − 0.33; 95% CI: − 0.44; − 0.23), but higher IgM levels (beta: 0.08; 95% CI: 0.04;0.13) than men. Former and particularly current smoking were associated with lower IgA and IgG (betas between − 0.07 and − 1.03). Higher alcohol consumption was associated with lower IgG (beta for heavy drinking: − 0.70; 95% CI: − 0.91; − 0.48). Corticosteroid use was associated with lower IgG (beta: − 1.12; 95% CI: − 1.58; − 0.66). Associations with cardiovascular factors were heterogeneous and differed between sexes. Conclusion Age, sex, smoking, alcohol consumption, corticosteroid use, and cardiovascular factors are determinants that should be considered when interpreting serum Ig levels in middle-aged and elderly individuals and may require adjusted reference ranges.

Menstrual Cycle Modulation of Benzodiazepines Effects in Healthy Women

2009 ◽  
Author(s):  
Shanna Babalonis ◽  
Joshua A. Lile ◽  
Catherine A. Martin ◽  
Thomas H. Kelly
Keyword(s):  
Menstrual Cycle ◽  
Healthy Women
Sign in / Sign up

Export Citation Format

Share Document