scholarly journals The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study

2021 ◽  
pp. 153473462110552
Author(s):  
Başak Büyük ◽  
Cemre Aydeğer ◽  
Yasemen Adalı ◽  
Hüseyin Avni Eroğlu
Keyword(s):  
Wound Healing ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Female Rats ◽  
Cell Infiltration ◽  
Fibroblast Proliferation ◽  
Accelerate Wound Healing ◽  
Wound Model ◽  
Tnf Α ◽  
Excisional Wound

Background: Wound healing has a vital importance for the organism and various agents are used to accelerate wound healing. Although the effect of boron on wound healing is known, its mechanisms are not completely clear yet. In this study, the effect of boron in the Ephrin /Eph pathway will be evaluated. Methods: Forty adult female rats were used in the study. A full-thickness excisional wound model was created in all groups divided as Control, Fito, Boron and Plu groups. After the applications performed twice a day and lasting 7 days, skin tissues obtained and evaluated histopathological (inflammatory cell infiltration, oedema, and fibroblast proliferation density) and immunohistochemical (TNF-α, EphrinA1, EphrinB1, EphrinB2 and EphB4). Results: Inflammatory cell infiltration score was found to be higher in the Fito group compared to Boron group (p = .018). Fibroblast proliferation density was higher in Plu group than Boron group (p = .012). While TNF-α was lower in boron group than Plu (p = .027) and Fito (p = .016) groups, EphrinA1 was higher in Boron group than Plu group (p = .005). EphrinB1 expression was higher in Boron group compared to Plu (p = .015) and Fito (p = .015) groups, and the same difference was also observed in EphrinB2 (p values .000). Similarly, EphB4 immunoreactivity was higher in the Boron group compared to Plu (p = .000) and Fito (p = .002). Conclusion: One of the mechanisms of action of boron in wound healing is to increase EphrinB1, EphrinB2 and EphB4. Low TNF-α and histopathological findings indicate that boron limits extensive wound healing.

scholarly journals The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study

2021 ◽  
Author(s):  
Başak Büyük ◽  
Cemre Aydeğer ◽  
Yasemen Adalı ◽  
Hüseyin Avni Eroğlu
Keyword(s):  
Wound Healing ◽  
Boric Acid ◽  
Inflammatory Cell ◽  
Female Rats ◽  
Accelerate Wound Healing ◽  
Wound Model ◽  
Tnf Α ◽  
Excisional Wound ◽  
Factor Α ◽  
Necrosis Factor

Abstract Background: Wound healing has a vital importance for the organism and various agents are used to accelerate wound healing. Although the effect of boron on wound healing is known, its mechanisms are not completely clear yet. In this study, the effect of boron in the Ephrin /Eph pathway will be evaluated.Methods and Results: Forty adult female rats were used in the study. A full-thickness excisional wound model was created in all groups divided as control, Fito cream, Boric acid and Pluronic gel groups. Histopathologically, inflammatory cell infiltration(ICI), edema and fibroblast proliferation density(FPD); immunohistochemically TNF-α(Tumor necrosis factor α), EphrinA1, EphrinB1, EphrinB2 and EphB4 antibodies were evaluated in the skin tissues obtained after the applications performed twice a day and lasting 7 days.The ICI score was found to be higher in the Fito group compared to the boron group(p=0.018). FPD was higher in Plu group than boron group(p=0.012). While TNF-α was lower in boron group than Plu(p=0.027) and Fito(p=0.016) groups, EphrinA1 was higher in Boron group than plu group(p=0.005). EphrinB1 expression was higher in Boron group compared to Plu(p=0.015) and Fito(p=0.015) groups, and the same difference was also observed in EphrinB2 (p values 0.000). Similarly, EphB4 immunoreactivity was higher in the Boron group compared to the plu(p=0.000) and Fito(p=0.002).Coclusion: One of the mechanisms of action of boron in wound healing is to increase EphrinB1, EphrinB2 and EphB4. Low TNF-α and histopathological findings indicate that boron limits extensive wound healing.

scholarly journals Topical Application of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) Accelerates Wound Healing in Diabetic Rabbits

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Abdulmohsen H. Al-Elq ◽  
Mir Sadat-Ali ◽  
Mohamed Elsharawy ◽  
Ibrahim Al-Habdan ◽  
Fatin Othman Al-Aqeel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Control Group ◽  
Collagen Deposition ◽  
Peptide Group ◽  
Diabetic Rabbits

Objective. Diminished wound healing is a common problem in diabetic patients due to diminished angiogenesis. SHMSP was found to promote angiogenesis. The present study was carried out to examine the effect of this peptide in healing of wounds in diabetic rabbits.Materials and Methods. Twenty male New Zealand rabbits were used in this study. Diabetes mellitus was induced and the rabbits were randomly divided into two equal groups: control group and peptide group. A-full thickness punch biopsy was made to create a wound of about 10 mm on the right ears of all rabbits. Every day, the wound was cleaned with saline in control groups. In the peptide group, 15 mg of SHMSP was applied after cleaning. On day 15th, all animals were sacrificed, and the wounds were excised with a rim of 5 mm of normal surrounding tissue. Histo-pathological assessment of wound healing, inflammatory cell infiltration, blood vessel proliferation, and collagen deposition was performed.Results. There were no deaths among the groups. There was significant increase in wound healing, blood vessel proliferation and collagen deposition, and significant decrease in inflammatory cell infiltration in the peptide group compared to the control group.Conclusion. Topical application of SHMSP improves wound healing in diabetic rabbits.

scholarly journals Histopathological study of Effects of continuous injection of dexamethasone on wound healing in rabbits

2011 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
F. Salim
Keyword(s):  
Wound Healing ◽  
Control Animal ◽  
Inflammatory Cell ◽  
Treated Group ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Histopathological Study ◽  
Continuous Injection ◽  
Made In

In this study 16 rabbit was divided in to two groups equally the first group was daily injected by dexamethasone(1 mg/Kg) for 21 days,the second group was left without any treatment as a control. An excision was made in the skin of rabbits and then sutured with silk band 2.5cm.The skin spicemen were taken for histopathological sections for wound healing evaluation at postoperative days 3,7and 14. The scars of the dexamethasone-treated group were less formation. The epithelization ,collagenization and the inflammatory cell infiltration was less intense in the dexamethasone- treated group in compare with the control animal.

scholarly journals The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice

2021 ◽  
Vol 8 (8) ◽  
pp. 167
Author(s):  
Enrico Gugliandolo ◽  
Francesco Macrì ◽  
Roberta Fusco ◽  
Rosalba Siracusa ◽  
Ramona D’Amico ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Biological Properties ◽  
Helix Aspersa ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
Wound Model ◽  
Tnf Α ◽  
Excisional Wound ◽  
Endothelial Growth Factor

Wound healing is a physiological process comprising several coordinated phases, such as inflammation, proliferation, and remodeling. For centuries, Helix aspersa Muller mucus has been known to have biological properties that are useful for treating skin disorders. In this study, we used a full-thickness excisional wound model in mice to test the hypothesis that Snail Secretion Filtrate (SSF) can improve the wound healing process. The mucus from Helix aspersa Muller was obtained mechanically by manually stimulating snails with a sterile cotton swab tip, and then the mucus was subjected to a series of filtrations to obtain SSF. After wounding, the mice were treated topically with SSF for 14 days. Our macroscopic results show that the SSF treatment significantly improved the speed and percentage of wound area closure. Furthermore, SSF improved several markers of proper wound healing, such as collagen deposition (Masson, COL3A1, matrix metalloproteinases (MMPs)) and the tissue remodeling process (α-sma, vascular-endothelial growth factor (VEGF)). SSF was also able to counteract the inflammatory process in injured wound tissue (myeloperoxidase (MPO) IL-1β, IL-6, TNF-α). In conclusion, our results show that SSF is able to enhance the speed and efficiency of wound healing and positively regulate several aspects of the wound healing process, such as the proliferative and remodeling phases.

scholarly journals Thymosin β4-sulfoxide attenuates inflammatory cell infiltration and promotes cardiac wound healing

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Mark A. Evans ◽  
Nicola Smart ◽  
Karina N. Dubé ◽  
Sveva Bollini ◽  
James E. Clark ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Thymosin Β4 ◽  
Cardiac Wound Healing

A Case of Bullous Amyloidosis Associated with an Inflammatory Cell Infiltration and Prominent Pruritus

2008 ◽  
Vol 70 (3) ◽  
pp. 269-273
Author(s):  
Taisuke KAMIYAMA ◽  
Yoshihiro KAWAGUCHI ◽  
Masami SASAKI ◽  
Masamichi SATOU ◽  
Kumiko MIURA ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration

scholarly journals The Symmetric 3D Organization of Connective Tissue around Implant Abutment: A Key-Issue to Prevent Bone Resorption

Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1126
Author(s):  
Giovanna Iezzi ◽  
Francesca Di Lillo ◽  
Michele Furlani ◽  
Marco Degidi ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Connective Tissue ◽  
Inflammatory Cell ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Collagen Fibers ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Oral Epithelium ◽  
Implant Abutment

Symmetric and well-organized connective tissues around the longitudinal implant axis were hypothesized to decrease early bone resorption by reducing inflammatory cell infiltration. Previous studies that referred to the connective tissue around implant and abutments were based on two-dimensional investigations; however, only advanced three-dimensional characterizations could evidence the organization of connective tissue microarchitecture in the attempt of finding new strategies to reduce inflammatory cell infiltration. We retrieved three implants with a cone morse implant–abutment connection from patients; they were investigated by high-resolution X-ray phase-contrast microtomography, cross-linking the obtained information with histologic results. We observed transverse and longitudinal orientated collagen bundles intertwining with each other. In the longitudinal planes, it was observed that the closer the fiber bundles were to the implant, the more symmetric and regular their course was. The transverse bundles of collagen fibers were observed as semicircular, intersecting in the lamina propria of the mucosa and ending in the oral epithelium. No collagen fibers were found radial to the implant surface. This intertwining three-dimensional pattern seems to favor the stabilization of the soft tissues around the implants, preventing inflammatory cell apical migration and, consequently, preventing bone resorption and implant failure. This fact, according to the authors’ best knowledge, has never been reported in the literature and might be due to the physical forces acting on fibroblasts and on the collagen produced by the fibroblasts themselves, in areas close to the implant and to the symmetric geometry of the implant itself.

Sign in / Sign up

Export Citation Format

Share Document