scholarly journals The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice

2021 ◽  
Vol 8 (8) ◽  
pp. 167
Author(s):  
Enrico Gugliandolo ◽  
Francesco Macrì ◽  
Roberta Fusco ◽  
Rosalba Siracusa ◽  
Ramona D’Amico ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Biological Properties ◽  
Helix Aspersa ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
Wound Model ◽  
Tnf Α ◽  
Excisional Wound ◽  
Endothelial Growth Factor

Wound healing is a physiological process comprising several coordinated phases, such as inflammation, proliferation, and remodeling. For centuries, Helix aspersa Muller mucus has been known to have biological properties that are useful for treating skin disorders. In this study, we used a full-thickness excisional wound model in mice to test the hypothesis that Snail Secretion Filtrate (SSF) can improve the wound healing process. The mucus from Helix aspersa Muller was obtained mechanically by manually stimulating snails with a sterile cotton swab tip, and then the mucus was subjected to a series of filtrations to obtain SSF. After wounding, the mice were treated topically with SSF for 14 days. Our macroscopic results show that the SSF treatment significantly improved the speed and percentage of wound area closure. Furthermore, SSF improved several markers of proper wound healing, such as collagen deposition (Masson, COL3A1, matrix metalloproteinases (MMPs)) and the tissue remodeling process (α-sma, vascular-endothelial growth factor (VEGF)). SSF was also able to counteract the inflammatory process in injured wound tissue (myeloperoxidase (MPO) IL-1β, IL-6, TNF-α). In conclusion, our results show that SSF is able to enhance the speed and efficiency of wound healing and positively regulate several aspects of the wound healing process, such as the proliferative and remodeling phases.

scholarly journals Role of 4-Hexylresorcinol in the Field of Tissue Engineering

2020 ◽  
Vol 10 (10) ◽  
pp. 3385 ◽  
Author(s):  
Jwa-Young Kim ◽  
Hyun Seok
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Bone Formation ◽  
Osteoclast Differentiation ◽  
Biological Properties ◽  
Vascular Endothelial ◽  
Phenolic Lipids ◽  
Tnf Α ◽  
Factor Α ◽  
Endothelial Growth Factor

4-hexylresorcinol (4-HR), as a derivative of phenolic lipids, has biological and pharmacological properties that are beneficial when used with a biomaterial. It has antimicrobial and antiseptic activity and can thus prevent contamination and infection of biomaterials. 4-HR suppresses the nuclear factor kappa B (NF-κB) signaling pathway related to osteoclast differentiation. The suppression of NF-κB increases the bone formation marker and contributes to new bone formation. The tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine produced by macrophages and suppressed by 4-HR. Suppression of TNF-α decreases osteoclast activity and promotes wound healing. 4-HR increases the vascular endothelial growth factor and has an anti-thrombotic effect. When incorporated into silk vascular patches, it promotes endothelium wound healing. Recently, 4-HR has exhibited biological properties and has been successfully incorporated into various biomaterials. Consequently, it is a useful pharmacological chemical that can be used with biomaterials in the field of tissue engineering.

scholarly journals Effects of inhibition of lymphangiogenesis by the vascular endothelial growth factor receptor 3 (VEGFR-3) inhibitor, MAZ51 on full thickness wounds in mice

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Kana Takada ◽  
Yukari Nakajima ◽  
Tamae Urai ◽  
Kanae Mukai ◽  
Kimi Asano ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Process ◽  
Lymphatic Vessels ◽  
Growth Factor Receptor ◽  
Control Group ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
Tissue Samples ◽  
Endothelial Growth Factor

We herein used MAZ51 to inhibit lymphangiogenesis and aimed to clarify the effect of inhibition of lymphangiogenesis on wound healing. BALB/c male mice were divided into two groups: the control group which was injected the dimethyl sulfoxide (DMSO), the experiment group was injected MAZ51 in the DMSO. All wounds were observed for 15 days and the wound areas were measured. Tissue samples were harvested on day 3, 7, 9, 11, 13 and 15, and subjected to immunostaining of blood vessels and lymphatic vessels. There are no significant differences between two groups in the wound area, the number of blood vessels and lymphatic vessels. The number of blood vessels peaked on day 7 in both groups as with previous studies, while the number of lymphatic vessels peaked on 13 or 15 in both groups. This result revealed delayed lymphangiogenesis in comparison with previous studies. The wound healing process in the control and experiment groups were similar, but both groups seemed delayed lymphangiogenesis comparing with previous studies. Injections of MAZ51 or/and DMSO did not affect angiogenesis, while they may affect lymphangiogenesis.

scholarly journals Increased Vascular Endothelial Growth Factor (VEGF) Expression in Wistar Rat Oral Mucosa Traumatic Ulcer due to Golden Sea Cucumber (Stichopus hermanni) Extract Gel

2019 ◽  
Vol 53 (6) ◽  
Author(s):  
Pratiwi Soesilawati ◽  
Eghia Laditra Ambarani ◽  
Yuliati ◽  
Aqsa Sjuhada Oki
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Sea Cucumber ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Endothelial Growth Factor

Background. Traumatic ulcer is a lesion formed by a local tissue damage due to trauma on epithelial layer. Vascular Endothelial Growth Factor (VEGF) plays an important role in wound healing, especially in angiogenesis. Golden sea cucumber (Stichopus hermanni) is believed to accelerate the wound healing process. Objective. To prove that golden sea cucumber extract can increase VEGF expression in oral mucous membrane traumatic ulcer in rat.Method. S. hermanni extract was prepared by freeze-dry method, then an extract was made using PEG 400 or PEG 4000 at 40% and 80% concentrations, respectively, and applied to the animal’s oral wounds. The animals were divided into three groups: control; 40% S. hermanni extract gel; and 80% S. hermanni extract gel. The ulcers that formed on day 3 were rubbed gently with S. hermanni extract gel. After being sacrificed on day 4, sample tissues from the lower lips were prepared for histopathology to count the number of VEGF expression. The results were analyzed using the One-Way ANOVA statistical test. Results. A significant increase in the VEGF expression of 80% concentration S. hermanni extract gel was found compared with those in the control group (p=0.00). Conclusion. Golden sea cucumber extract (Stichopus hermanni) gel increased the VEGF expression in oral mucous traumatic ulcer.

scholarly journals Reparative Processes in Soft Tissues. Influence of Acidity

2017 ◽  
Vol 10 (1) ◽  
pp. 64-71
Author(s):  
Alexander Alekseevich Andreev ◽  
Anton Petrovich Ostroushko ◽  
Artyom Olegovich Chuyan ◽  
Artyom Romanovich Karapityan
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Soft Tissues ◽  
Ph Value ◽  
Healing Process ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
The Usa ◽  
Endothelial Growth Factor ◽  
Influence Degree

Wound treatment is one of the most actual problems of modern surgery due to the increase of degree of injury, and also due to a sharp increase in quantity of emergencies of natural and technogenic disasters. Injuries of soft tissues of various etiology are annually diagnosed in the USA more than for 30 million people. To date there is a deep theoretical base developed for understanding of a pathophysiology of wound healing process, however still many questions remain debatable. The regeneration process is characterized by high metabolic activity and is based on biochemical reactions for which the pH value is of great importance. Changes of pH values of a wound during wound healing process are reflected in article. The authors noted the influence of pH on proteases and its inhibitors activity, expression of vascular endothelial growth factor (VEGF), fibroblast activity, keratinocytes proliferation, microbial expansion, and also on wound oxygenation; in response to invasion and dissemination of microorganisms. Materials of the researches that determined pH influence degree on the skin graft survival are represented. Generalizing, following conclusions can be done: pH performs as a modulator in healing both acute chronic wounds; acidic milieu in the inflammation phase and the first half of the proliferation phase occurs to the most conducive, positively affecting on necrolysis and granulation formation; alkaline milieu in the second half of inflammation phase and in phase of epithelization  promotes early closing of the tissue defect.

scholarly journals Polymer-Based Scaffolds Loaded with Aloe vera Extract for the Treatment of Wounds

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 961
Author(s):  
Sibusiso Alven ◽  
Vuyolwethu Khwaza ◽  
Opeoluwa O. Oyedeji ◽  
Blessing A. Aderibigbe
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Mechanical Performance ◽  
Healing Process ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
Biological Properties ◽  
Wound Management ◽  
Wound Healing Process ◽  
Treatment Of Wounds

The treatment of wounds is one challenging biomedical field due to delayed wound healing common in chronic wounds. Several factors delay wound healing, including microbial infections, malnutrition, underlying physiological conditions, etc. Most of the currently used wound dressing materials suffer from poor antimicrobial properties, poor biodegradability and biocompatibility, and weak mechanical performance. Plant extracts, such as Aloe vera, have attracted significant attention in wound management because of their interesting biological properties. Aloe vera is composed of essential constituents beneficial for the wound healing process, such as amino acids, vitamins C and E, and zinc. Aloe vera influences numerous factors that are involved in wound healing and stimulates accelerated healing. This review reports the therapeutic outcomes of aloe vera extract-loaded polymer-based scaffolds in wound management.

scholarly journals Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness

2021 ◽  
Vol 12 ◽  
Author(s):  
Laryssa C. Manigat ◽  
Mitchell E. Granade ◽  
Suchet Taori ◽  
Charlotte Anne Miller ◽  
Luke R. Vass ◽  
...  
Keyword(s):  
Wound Healing ◽  
T Cell ◽  
Healing Process ◽  
Cell Activity ◽  
Regulatory Function ◽  
Burned Area ◽  
Wound Healing Process ◽  
Wound Model ◽  
Complex Wound

The diacylglycerol kinases (DGKs) are a family of enzymes responsible for the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). In addition to their primary function in lipid metabolism, DGKs have recently been identified as potential therapeutic targets in multiple cancers, including glioblastoma (GBM) and melanoma. Aside from its tumorigenic properties, DGKα is also a known promoter of T-cell anergy, supporting a role as a recently-recognized T cell checkpoint. In fact, the only significant phenotype previously observed in Dgka knockout (KO) mice is the enhancement of T-cell activity. Herein we reveal a novel, macrophage-specific, immune-regulatory function of DGKα. In bone marrow-derived macrophages (BMDMs) cultured from wild-type (WT) and KO mice, we observed increased responsiveness of KO macrophages to diverse stimuli that yield different phenotypes, including LPS, IL-4, and the chemoattractant MCP-1. Knockdown (KD) of Dgka in a murine macrophage cell line resulted in similar increased responsiveness. Demonstrating in vivo relevance, we observed significantly smaller wounds in Dgka-/- mice with full-thickness cutaneous burns, a complex wound healing process in which macrophages play a key role. The burned area also demonstrated increased numbers of macrophages. In a cortical stab wound model, Dgka-/- brains show increased Iba1+ cell numbers at the needle track versus that in WT brains. Taken together, these findings identify a novel immune-regulatory checkpoint function of DGKα in macrophages with potential implications for wound healing, cancer therapy, and other settings.

Exercise accelerates cutaneous wound healing and decreases wound inflammation in aged mice

2008 ◽  
Vol 294 (1) ◽  
pp. R179-R184 ◽  
Author(s):  
K. Todd Keylock ◽  
Victoria J. Vieira ◽  
Matthew A. Wallig ◽  
Luisa A. DiPietro ◽  
Megan Schrementi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Moderate Intensity ◽  
Wound Healing Process ◽  
Indirect Measure ◽  
Monocyte Chemoattractant Protein 1 ◽  
Aged Mice ◽  
Wound Size ◽  
Tnf Α ◽  
Old Mice

The purpose of this study was to determine the effect of exercise on wound healing and inflammation in young (3 mo) and old (18 mo) female BALB/cByJ mice. Mice were assigned to either exercise or sedentary control (control) groups. The exercise group mice were run on a motorized treadmill at a moderate intensity 30 min/day for 8 days. All mice were given four full-thickness dermal wounds, and the rate of wound closure was assessed daily for 10 days. Four months later, the aged mice were rerandomized to treatment, wounded again in different locations, and wounds were harvested at 1, 3, or 5 days postwounding. Wound tissue was analyzed for IL-1β, IL-6, keratinocyte chemoattractant (KC), monocyte chemoattractant protein-1 (MCP-1), and TNF-α protein. Myeloperoxidase (MPO) activity and F4/80 mRNA were assessed as an indirect measure of neutrophil and macrophage content, respectively. There was a trend ( P = 0.10) for exercise to reduce wound size in young mice, and exercise significantly ( P < 0.05) decreased wound size in old mice. TNF-α, KC, and MCP-1 were significantly ( P < 0.05) lower in wounds from exercised old mice compared with control. No group differences were found for wound IL-1β or IL-6, MPO activity, or F4/80 mRNA. Our data suggest that exercise accelerates the wound healing process in old mice. This improved healing response in the old mice may be the result of an exercise-induced anti-inflammatory response in the wound.

scholarly journals The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study

2021 ◽  
pp. 153473462110552
Author(s):  
Başak Büyük ◽  
Cemre Aydeğer ◽  
Yasemen Adalı ◽  
Hüseyin Avni Eroğlu
Keyword(s):  
Wound Healing ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Female Rats ◽  
Cell Infiltration ◽  
Fibroblast Proliferation ◽  
Accelerate Wound Healing ◽  
Wound Model ◽  
Tnf Α ◽  
Excisional Wound

Background: Wound healing has a vital importance for the organism and various agents are used to accelerate wound healing. Although the effect of boron on wound healing is known, its mechanisms are not completely clear yet. In this study, the effect of boron in the Ephrin /Eph pathway will be evaluated. Methods: Forty adult female rats were used in the study. A full-thickness excisional wound model was created in all groups divided as Control, Fito, Boron and Plu groups. After the applications performed twice a day and lasting 7 days, skin tissues obtained and evaluated histopathological (inflammatory cell infiltration, oedema, and fibroblast proliferation density) and immunohistochemical (TNF-α, EphrinA1, EphrinB1, EphrinB2 and EphB4). Results: Inflammatory cell infiltration score was found to be higher in the Fito group compared to Boron group (p = .018). Fibroblast proliferation density was higher in Plu group than Boron group (p = .012). While TNF-α was lower in boron group than Plu (p = .027) and Fito (p = .016) groups, EphrinA1 was higher in Boron group than Plu group (p = .005). EphrinB1 expression was higher in Boron group compared to Plu (p = .015) and Fito (p = .015) groups, and the same difference was also observed in EphrinB2 (p values .000). Similarly, EphB4 immunoreactivity was higher in the Boron group compared to Plu (p = .000) and Fito (p = .002). Conclusion: One of the mechanisms of action of boron in wound healing is to increase EphrinB1, EphrinB2 and EphB4. Low TNF-α and histopathological findings indicate that boron limits extensive wound healing.

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