Green Nanotechnology from Brassicaceae

2013 ◽  
Vol 1 ◽  
pp. 194308921350947 ◽  
Author(s):  
Menka Khoobchandani ◽  
Ajit Zambre ◽  
Kavita Katti ◽  
Chung-Ho Lin ◽  
Kattesh V. Katti
Keyword(s):  
Mass Spectrometry ◽  
Gold Nanoparticles ◽  
Biological Fluids ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Therapeutic Effects ◽  
Cytotoxic Effects ◽  
Liquid Chromatography Tandem Mass ◽  
In Vitro Stability

The interaction of cocktail of phytochemicals from broccoli with gold salt results in dual reduction and surface capping to produce well-defined stable and biocompatible gold nanoparticles (B-AuNPs). Broccoli phytochemicals–coated gold nanoparticles (B-AuNPs) have been fully characterized. Detailed in vitro stability in various biological fluids and affinity and selectivity for tumor cells have been investigated. The B-AuNPs showed significant in vitro cytotoxic effects against various cancer cells (MDA-MB-231, PC-3, U266, SkBr3, and T47D) as confirmed by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) and flow cytometry apoptosis assays. Surface encapsulation of cocktail of broccoli phytochemicals on AuNPs facilitates the cellular internalization, thereby validating the in vitro therapeutic effects of these nanoparticles. Detailed analyses performed by combination of gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS–MS) have confirmed the presence of biologically active phytochemicals including glucoraphanin, phenethyl glucosinolates, quercetin, folic acid, vitamin C, allyl isothiocyanates, 2-phenylethyl isothiocyanates, and sulforaphane. The unique synergistic cocktail effects of B-AuNPs will provide new opportunities for generating biocompatible AuNPs for molecular imaging and therapeutic applications.

scholarly journals Identification of in vitro and in vivo potential metabolites of novel cardiovascular and adrenolytic drugs by liquid chromatography-mass spectrometry with the aid of experimental design

2019 ◽  
Vol 18 (2) ◽  
pp. 179-194
Author(s):  
Malgorzata Szultka-Mlynska ◽  
Katarzyna Pauter ◽  
Boguslaw Buszewski
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Phase Ii ◽  
Liver Microsomes ◽  
Biologically Active ◽  
Rat Liver Microsomes ◽  
Gas Temperature ◽  
Liquid Chromatography Tandem Mass

Abstract Drug metabolism in liver microsomes was studied in vitro using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Relevant drug was incubated with dog, human and rat liver microsomes (DLMs, HLMs, RLMs) along with NADPH, and the reaction mixture was analyzed by LC-MS/MS to obtain specific metabolic profile. GRACE analytical C18 column, Vision HT (50 × 2 mm, 1.5 μm) was implemented with acetonitrile and water (+ 5 mM ammonium acetate) in a gradient mode as the mobile phase at a flow 0.4 mL.min−1. Different phase I and phase II metabolites were detected and structurally described. The metabolism of the studied drugs occurred via oxidation, hydroxylation and oxidative deamination processes. Conjugates with the glucuronic acid and sulfate were also observed as phase II biotransformation. The central composite design (CCD) showed that factors, such as time incubation, liver microsomal enzymes concentration and NADPH concentration, along with drying gas temperature, nebulizer gas pressure and capillary voltage significantly affected the final response of the method. This study describes the novel information about the chemical structure of the potential metabolites of selected biologically active compounds, which provide vital data for further pharmacokinetic and in vivo metabolism studies.

scholarly journals A study of in vitro metabolism and cytotoxicity of mephedrone and methoxetamine in human and pig liver models using GC/MS and LC/MS analyses

2020 ◽  
Vol 18 (1) ◽  
pp. 1507-1522
Author(s):  
Majed Alshamaileh ◽  
Issam Hussain ◽  
Mark Baron ◽  
Ruth Croxton ◽  
Marleen Vetter ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Death ◽  
Cell Lines ◽  
Liver Microsomes ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Effects ◽  
Pig Liver ◽  
Chromatography Mass Spectrometry ◽  
Optimized Conditions

AbstractIn the current study, the metabolism of two novel psychoactive substances (NPSs), mephedrone and methoxetamine (MXE), was studied in vitro in pig liver microsomes to determine potential metabolites by liquid chromatography-mass spectrometry (LC-MS). Later, in vitro studies were performed using HepaRG™ cells to determine the human metabolites of these drugs using gas chromatography-mass spectrometry (GC-MS). The aim of the study was to detect metabolites from the metabolic mixture in the human cell lines using GC-MS, since this is a more readily available technique within forensic laboratories. Microsomes were prepared through a conventional ultracentrifugation method and incubated under optimized conditions with the drugs for 3 h. Subsequently, the samples were investigated using LC-MS. A similar methodology was then applied in the HepaRG™ cells, and the GC-MS conditions were optimized using N,O-bis(trimethylsilyl)trifluoroacetamide as a derivatization agent. The analysis showed two molecules from a successful in vitro metabolism, namely, hydroxytoly-mephedrone and nor-dihydro mephedrone. For MXE, two metabolites are presented produced by the O-demethylation and reduction of the ketone moiety to the corresponding alcohol, respectively. Using the human HepaRG™ cells, only nor-dihydro mephedrone could be identified by GC-MS. Since hydroxytoly-mephedrone and the MXE metabolites are more polar, it is suggested that GC-MS even with derivatization may not be suitable. In addition, cytotoxicity was studied utilizing HepaRG™ cell lines. The drugs show cytotoxic effects causing in vitro cell death, within the specified range of EC50 0.3211 mM (79 μg/mL) and 0.6297 mM (111 μg/mL) for mephedrone and MXE, respectively. These drugs were able to cause 73–84% cell death.

scholarly journals Holospiniferoside: A New Antitumor Cerebroside from The Red Sea Cucumber Holothuria spinifera: In Vitro and In Silico Studies

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1555
Author(s):  
Enas E. Eltamany ◽  
Usama Ramadan Abdelmohsen ◽  
Dina M. Hal ◽  
Amany K. Ibrahim ◽  
Hashim A. Hassanean ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Red Sea ◽  
Active Site ◽  
Sea Cucumber ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometric Analysis ◽  
Chemical Structures ◽  
In Silico Studies ◽  
Red Sea Cucumber

Chemical investigation of the methanolic extract of the Red Sea cucumber Holothuria spinifera led to the isolation of a new cerebroside, holospiniferoside (1), together with thymidine (2), methyl-α-d-glucopyranoside (3), a new triacylglycerol (4), and cholesterol (5). Their chemical structures were established by NMR and mass spectrometric analysis, including gas chromatography–mass spectrometry (GC–MS) and high-resolution mass spectrometry (HRMS). All the isolated compounds are reported in this species for the first time. Moreover, compound 1 exhibited promising in vitro antiproliferative effect on the human breast cancer cell line (MCF-7) with IC50 of 20.6 µM compared to the IC50 of 15.3 µM for the drug cisplatin. To predict the possible mechanism underlying the cytotoxicity of compound 1, a docking study was performed to elucidate its binding interactions with the active site of the protein Mdm2–p53. Compound 1 displayed an apoptotic activity via strong interaction with the active site of the target protein. This study highlights the importance of marine natural products in the design of new anticancer agents.

scholarly journals Identifying volatile in vitro biomarkers for oral bacteria with proton-transfer-reaction mass spectrometry and gas chromatography–mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kajsa Roslund ◽  
Markku Lehto ◽  
Pirkko Pussinen ◽  
Kari Hartonen ◽  
Per-Henrik Groop ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Oral Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Analysis ◽  
Bacterial Volatiles

AbstractWe have measured the volatile fingerprints of four pathogenic oral bacteria connected to periodontal disease and dental abscess: Porphyromonas gingivalis (three separate strains), Prevotella intermedia, Prevotella nigrescens and Tannerella forsythia. Volatile fingerprints were measured in vitro from the headspace gas of the bacteria cultured on agar. Concrete identification of new and previously reported bacterial volatiles were performed by a combination of solid phase microextraction (SPME) and offline gas chromatography–mass spectrometry (GC–MS). We also studied the effect of the reduced electric field strength (E/N) on the fragmentation patterns of bacterial volatiles in online proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). We aimed to discover possible new biomarkers for the studied oral bacteria, as well as to validate the combination of GC–MS and PTR-MS for volatile analysis. Some of the most promising compounds produced include: 1-Methyl-1,2,3,4-tetrahydroisoquinoline (1MeTIQ), indole, and a cascade of sulphur compounds, such as methanethiol, dimethyl disulphide (DMDS) and dimethyl trisulphide (DMTS). We also found that several compounds, especially alcohols, aldehydes and esters, fragment significantly with the PTR-MS method, when high E/N values are used. We conclude that the studied oral bacteria can be separated by their volatile fingerprints in vitro, which could have importance in clinical and laboratory environments. In addition, using softer ionization conditions can improve the performance of the PTR-MS method in the volatile analysis of certain compounds.

scholarly journals High cortisol and cortisone levels are associated with breast milk dioxin concentrations in Vietnamese women

2014 ◽  
Vol 170 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Teruhiko Kido ◽  
Tung Van Dao ◽  
Manh Dung Ho ◽  
Nhu Duc Dang ◽  
Ngoc Thien Pham ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Breast Milk ◽  
Steroid Hormones ◽  
Hot Spot ◽  
Serum Cortisol ◽  
Epidemiological Studies ◽  
Gas Chromatography Mass Spectrometry ◽  
Vietnamese Women ◽  
Liquid Chromatography Tandem Mass ◽  
Dioxin Exposure

ObjectiveDioxin (polychlorinated dibenzodioxins+polychlorinated dibenzofurans) is one of the most toxic chemical substances known. Although it is suspected to cause endocrine disruption, very few epidemiological studies have been carried out on its effects on human steroid hormones. The aim of this study was to elucidate the association of dioxin exposure with steroid hormone levels in the saliva and serum of Vietnamese women.Study designTwo areas, namely Phu Cat (hot spot) and Kim Bang (nonexposed area), were selected for the study. The study subjects consisted of 51 and 58 women respectively. Saliva, blood, and breast milk samples were collected from the subjects in both the areas.MethodsCortisol, cortisone, DHEA, androstenedione, estrone, and estradiol levels in serum and saliva were determined by liquid chromatography–tandem mass spectrometry; dioxin concentrations in breast milk were measured by gas chromatography–mass spectrometry.ResultsDioxin concentrations in the breast milk of women from the dioxin hot spot were three to four times higher than those in the breast milk of women from the nonexposed area. Good correlations were found between the levels of six steroid hormones in saliva and those in serum respectively. Salivary and serum cortisol and cortisone levels in women from the dioxin hot spot were significantly higher than those in women from the nonexposed area (P<0.001) and those in all the subjects were positively associated with dioxin concentrations in Vietnamese women (P<0.01).ConclusionThese results suggest that dioxin influences steroidogenesis in humans. Saliva samples can be used for hormone analysis and are therefore excellent specimens in epidemiological studies.

Gas chromatography-mass spectrometry fingerprint and in vitro cytotoxic studies of Rubus steudneri leaf fractions against michigan cancer foundation-7 breast cancer cell line

2021 ◽  
Vol 17 (5) ◽  
pp. 54
Author(s):  
RaghavendraLakshmana Shetty Hallur ◽  
ChaitanyaV. N L. Motamarri ◽  
PrashithKekuda T. Ramamoorthy ◽  
ChetanD Murthy ◽  
RavikumarPatil H. Siddappa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Studies
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