scholarly journals Histological and immunohistochemical evaluation of mandibular bone tissue regeneration

2018 ◽  
Vol 32 ◽  
pp. 205873841879824 ◽  
Author(s):  
S Di Carlo ◽  
F De Angelis ◽  
E Brauner ◽  
D Rosella ◽  
P Papi ◽  
...  
Keyword(s):  
Bone Biopsy ◽  
Immunohistochemical Analysis ◽  
Bone Tissue Regeneration ◽  
Histological Evaluation ◽  
Mandibular Bone ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Autologous Bone ◽  
Age Range

The purpose of the study was to perform an immunohistochemical and histological evaluation of samples taken from different bone regeneration procedures in atrophic human mandible. 30 patients (15 men and 15 women, age range of 35–60 years), non-smokers, with good general and oral health were recruited in this study and divided into three groups. The first group included patients who were treated with blood Concentration Growth Factors (bCGF), the second group included patients who were treated with a mixture of bCGF and autologous bone, while the third group of patients was treated with bCGF and tricalcium phosphate/hydroxyapatite (TCP-HA). Six months after the regenerative procedures, all patients undergone implant surgery, and a bone biopsy was carried out in the site of implant insertion. Each sample was histologically and immunohistochemically examined. Histological evaluation showed a complete bone formation for group II, partial ossification for group I, and moderate ossification for group III. Immunohistochemical analysis demonstrated a statistically significant difference between the three groups, and the best clinical result was obtained with a mixture of bCGF and autologous bone.

scholarly journals Hepatotoxic Assessment of Pecralima nitida Seeds Supplemented Diet in Wistar Rats.

2017 ◽  
pp. 26-30
Author(s):  
C. Chukwu Ezinne, U. Osuocha Kelechi S. Ezekwe Ahamefula
Keyword(s):  
Liver Enzymes ◽  
Group Iv ◽  
Histological Evaluation ◽  
Albino Rats ◽  
Group Iii ◽  
Enzymes Activities ◽  
Group I ◽  
Significant Difference ◽  
Normal Rat ◽  
Group Ii

This study assessed phytochemical constituents of Pecralima nitida seed extract and its effect on liver enzymes activities of male albino rats fed Pecralima nitida seed supplemented diet. A total of twenty male albino rats were used for this study and were randomly divided into four groups of five rats each. Group I was fed with normal rat feed and water, group II was fed with 50% pecralima nitida seed and 50% normal rat feed, group III was fed with  70%  pecralima nitida seed and 30% normal rat feed while group IV was fed with 90% sample and 10% normal rat feed for a period of twenty eight days. The preliminary phytochemical profile showed the presence of flavoniod, saponin, tainins, glycoside, alkaloid, phenol and steroid. These bioactive compounds may contribute to the reputed medicinal efficacy of pecralima nitida seed. Liver enzymes activities such as AST showed no significant difference between the control (24.39 ± 3.6IU/L) and group II (25.88 ± 3.7IU/L) but significantly increased in group III (37.38 ± 7.2IU/L) and group IV (42.19±2.1IU/L). The other enzymes ALT and ALP showed significant statistical increase in groups II-IV (P<0.05). The histological evaluation shows that group III and IV had evidence of degenerative tissues induced by 70% sample and 30% normal rat feed and 90% and 10% normal rat feed.  This however showed and suggested that irrespective of the reputed medicinal relevance of pecralima nitida seed, care should be taken in the quantity of these extract that is consumed as this may exhibit cumulative toxicity leading to functional  impairment in the integrity of the liver.

Role of Cord Blood Carboxyhemoglobin in Detecting Significant Hyperbilirubinemia in Term Neonates with ABO Alloimmunization

2021 ◽  
Author(s):  
Mahir Tıraş ◽  
Emrah Can ◽  
Şahin Hamilçıkan
Keyword(s):  
Cord Blood ◽  
Total Serum ◽  
Healthy Controls ◽  
Term Neonates ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Positive Direct ◽  
Severe Hyperbilirubinemia

Objective This study aimed to assess whether cord blood carboxyhemoglobin (COHb) levels in jaundiced term neonates with and without a positive direct Coombs test (DCT) and in healthy controls could be used as a predictor of severe hyperbilirubinemia. The percentage of cord blood COHb should be higher among neonates with Coombs-positive ABO hemolytic disease than among those with Coombs-negative ABO incompatibility and higher than that of ABO-compatible control neonates. Study Design This cross-sectional descriptive study of 198 term neonates comprised three subgroups: group I featured 68 DCT-positive ABO-incompatible neonates (ABO + DCT), group II featured 60 DCT-negative ABO-incompatible neonates with hyperbilirubinemia (ABO–DCT), and group III featured 70 healthy controls. COHb was determined by an OSM3 hemoximeter. Results Group I differed from groups II and III for cord blood bilirubin, cord blood hemoglobin, and cord blood hematocrit. Groups I and II had higher mean total serum bilirubin (TSB) levels than group III, while there was no difference in the mean TSB levels between groups I and II. There was no significant difference between the COHb group means for groups I, II, and III (p = 0.98). The area under the receiver operating characteristic curve calculated for group I/group III and group II/group III were found to be 0.62 and 0.54, respectively. Conclusion COHb levels did not prove to be superior to the DCT for predicting the risk of developing severe hyperbilirubinemia in term neonates. Key Points

Effect of Silver Nitrate Application on the Success of Endoscopic Dacryocystorhinostomy

2021 ◽  
pp. 194589242110427
Author(s):  
Yaser S. Çetin ◽  
Ramazan Akın ◽  
Ufuk Düzenli ◽  
Mahfuz Turan ◽  
Nazım Bozan
Keyword(s):  
Silver Nitrate ◽  
Success Rate ◽  
Postoperative Period ◽  
Revision Surgery ◽  
Granuloma Formation ◽  
Stent Insertion ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Silicone Stent

Objectives To compare the clinical outcomes of patients undergoing additional procedures in endoscopic endonasal dacryocystorhinostomy (End-DCR) surgery and discuss the factors affecting the success of End-DCR surgery in light of relevant literature. Methods The study included 155 patients who underwent End-DCR surgery in our clinic due to epiphora. This was a prospective randomized, single-blind, controlled trial. Group I (control) included 54 patients who did not undergo silicone stent insertion or silver nitrate application, group II included 51 patients who underwent silicone stent insertion only, and group III included 50 patients who underwent silver nitrate application only. Statistical analysis was performed on follow-up data regarding anatomic and functional success rates. Results Revision surgery was performed in 16 patients who developed persistent epiphora in the postoperative period, including 6 in group I, 7 in group II, and 3 in group III ( P = .4). The most common reason for revision surgery was stenosis of the neo-ostium ( n = 8), followed by granulation tissue formation ( n = 5) and synechia formation ( n = 3). Granuloma formation was the most common postoperative complication, and a significant difference was found among the groups with regard to granuloma formation ( P = .04). At postoperative month 12, the functional success rate was estimated to be 88%, 86%, and 94%, and the anatomic success rate was estimated to be 94%, 92%, and 96% in groups I, II, and III, respectively, with no significant difference, found among the 3 groups with regard to both rates ( P = .79 and P = .76, respectively). Conclusion The results indicated that stenting and silver nitrate application did not affect surgical success. Our preliminary results on silver nitrate cauterization showed that it is an effective, inexpensive, and practical method to reduce granulation formation in the postoperative period.

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

scholarly journals Comparative Analysis of Acid-base Status Parameters in Newborn with Congenital Surgical Malformations During Perioperative Care Based on Types of Anesthesia

2021 ◽  
Vol 2 ◽  
pp. 14-22
Author(s):  
Oleksii Vlasov
Keyword(s):  
Regional Anesthesia ◽  
Oxygen Mixture ◽  
Acid Base ◽  
Group Iii ◽  
Group I ◽  
Anesthesia Group ◽  
Significant Difference ◽  
Combined Anesthesia ◽  
Acid Base Status ◽  
Group Ii

Introduction: Congenital malformations (CM) are most common in newborns and infants in the first year of life and require surgical correction in the first hours, days, months of life. Surgical interventions in severely ill babies with malformations can lead to catabolic stress, circulatory and respiratory disorders, metabolism shifts, water-electrolyte, protein, and acid-base status disorders. This study aimed to compare acid-base status in newborns and infants with congenital surgical pathology under different types of combined anesthesia.  Materials and methods: This retrospective study included 150 newborns and infants with CM who required surgery. The patients were divided into three groups based on types of provided anesthesia: inhalation by Sevoran (sevoflurane) and regional anesthesia (group I); inhalation of Sevoran and intravenous anesthesia by Fentanyl (group II); and intravenous combination of Fentanyl and 20% Sodium Oxybutyrate (group III). The analysis included: acid-base status, peripheral oximetry, and the need for an oxygen mixture inhaled by the patient. Results In group I, there was a significant reduction in partial tension of CO2 and increased pH from the pre-surgical status, at the time of induction of anesthesia, during the most painful, traumatic stage, and after surgery compared to group II and III. Peripheral O2 saturation was not critically reduced at all stages of observation except in babies of group I compared to group III at the stage of induction of anesthesia (97.79 ± 2.45 vs. 98.79 ± 1.63, p = 0.0194) and at the most painful period of surgery (96.29 ± 3.47 vs. 98.10 ± 2.47, p = 0.0368). In group I, newborns and infants required higher oxygen concentrations in the inhalation mixture. There was a significant difference in FiO2 between groups I and III during the most painful stage of surgery (0.47 ± 0.29 and 0.33 ± 0.2, p = 0.0071), and immediately after surgery (0.34 ± 0, 19 and 0.26 ± 0.13, p = 0.0246). Conclusion: Among the newborns and infants with CM requiring surgical intervention and combined anesthesia, the most substantial acid-base status changes were observed in the group where anesthesia was provided by Sevoran (sevoflurane) and regional anesthesia (Group I).

scholarly journals Hepatotoxicity and Histological Evaluation of Aqueous and Methanolic Leaf Extracts of Thaumatococcus Daniellii and Alchornea Cordifolia in Wistar Rat Models

2019 ◽  
Vol 1 (2) ◽  
pp. p42
Author(s):  
Service @ Ideasspread.org ◽  
Okafor I. J. ◽  
Nweke E. O. ◽  
Ewa O.
Keyword(s):  
Normal Control ◽  
Wistar Rat ◽  
Normal Control Group ◽  
Histological Evaluation ◽  
Control Group ◽  
Significant Elevation ◽  
Leaf Extracts ◽  
Group Iii ◽  
Methanolic Extracts ◽  
Group I

This study was carried out to ascertain the hepatotoxic potential of T.daniellii (T.d) and A. cordifolia (A.c). Investigations were conducted using standard methods. Oral administration of 200mg/kg aqueous leaf extracts of T.daniellii caused a non-significant increase in the activity of ALT (5.43±0.60IU/L), AST (16.93±0.26 IU/L) and ALP (160.70±1.04 IU/L) compared to the values recorded on the normal control (group I) ALT (3.84±0.16 IU/L), AST (14.19±0.52 IU/L) and ALP (157.26±0.64 IU/L). Group III administered with 200mg/kg methanolic leaf extract of T. daniellii manifested a significant elevation in the activity of ALT (13.15±0.89 IU/L), AST (22.84±0.38 IU/L) and ALP (170.40±0.44 IU/L) compared to the normal control. Similarly, groups IV and V which were orally administered with 200mg/kg aqueous and methanolic leaf extracts of A. cordifolia showed significant increase in the activity of ALT (6.32±0.33U/L), AST (17.70±0.030U/L) and ALP (161.13±0.09U/L) and ALT (7.55±0.59U/L), AST (19.35±0.26U/L) and ALP (165.38±0.35U/L) respectively compared to the values recorded on the control (group I). In conclusion, drug development protocols involving T. daniellii leaf should preferably use water as an ideal solvent. On the other hand, the hepatotocity associated with both aqueous and methanolic extracts of A. cordifolia could imply the presence of hepatotoxins in the leaf of the said plant.

scholarly journals Effects of clomiphene citrate plus estradiol or progesterone on endometrial ultrastructure: An RCT

2020 ◽  
Author(s):  
Robabeh Taheripanah ◽  
Maryam Kabir-Salmani ◽  
Masoomeh Favayedi ◽  
Marzieh Zamaniyan ◽  
Narges Malih ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Menstrual Cycle ◽  
Clomiphene Citrate ◽  
Endometrial Biopsy ◽  
Estradiol Valerate ◽  
Serum Progesterone ◽  
Group Iii ◽  
Estrogenic Effects ◽  
Group I ◽  
Significant Difference

Background: Pinopods concentrations in endometrial surface is a marker of implantation. Estradiol valerate (EV) was used to change the adverse effects of Clomiphene Citrate (CC) on the endometrium. Objective: The goal was to assess whether there is a significant difference in the endometrial pinopods concentrations and other parameters after adding EV and progesterone to higher doses of CC. Materials and Methods: In this prospective randomized clinical trial, a total of 30 women who did not respond to 100 mg of CC from February 2016 to June 2016 were evaluated. They were divided into three groups: group I) received 150 mg of CC alone, group II) CC with EV, and group III) CC plus progesterone. On day 21 of the menstrual cycle, endometrial biopsy, a blood sampling, and a scanning by electron microscopy were performed. Results: On day 21 of the menstrual cycle, there was no significant difference in the pinopods concentrations (p = 0.641) and serum estrogen levels (p = 0.276) between groups. However, the Serum progesterone levels in group I was higher than the other two groups (p = 0.007) in the same day. Conclusion: Since the addition of EV and progesterone to higher dosages of CC did not change the pinopods concentration and serum estrogen levels on day 21 of the menstrual cycle, and the serum progesterone levels was higher in CC alone group (i.e. group I) compared to other groups, it can be concluded that the anti-estrogenic effects of CC just appear on the endometrium and not on the plasma levels. Key words: Ovulation induction, Clomiphene, Estradiol, Progesterone, Electron microscopy, Endometrium.

scholarly journals Assessment of Outcome of Intrathecal Analgesia in Multiparous Women Undergoing Vaginal Delivery

2020 ◽  
Vol 5 (1) ◽  
pp. 153-155
Author(s):  
Sanjay Melville Masih ◽  
Rakesh Kumar Gupta
Keyword(s):  
Vaginal Delivery ◽  
Group Iv ◽  
Normal Vaginal Delivery ◽  
Secondary Outcome ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Multiparous Women ◽  
Group Ii ◽  
Intrathecal Analgesia

Background: The present study was conducted to assess the outcome of intrathecal analgesia in multiparous women undergoing vaginal delivery. Subjects and Methods: The present study was conducted among 80 multiparous women ages ranged 18- 40 years. All patients received 0.5 ml of intrathecal injection of 2.5 mg bupivacaine 0.5% and 1 ml dexamethasone 4 mg plus a 0.5 ml adjuvant. The adjuvants in group I patients were 100 μg morphine, 25 μg fentanyl in group II, 5 μg dexmedetomidine in group III and normal saline in group IV (control group). The primary and secondary outcome was the duration of pain relief, the analgesia onset time, the maximum level of sensory block, the visual analogue scale (VAS) was recorded. Results: The mean duration of analgesia in group I was 182.4 minutes, in group II was 170.5 minutes, in group III was 200.4 minutes and in group, IV was 140.2 minutes. The onset of analgesia was 3.9 minutes, 2.8 minutes, 2.7 minutes and 4.5 minutes in group I, II, III and IV respectively. S1 sensory regression time (minutes) was 181.4, 157.4, 185.3 and 130.6 in group I, II, III and IV respectively. Modified Bromage scale 5 minutes, 15 minutes and 30 minutes after IT in all groups was 0.0. VAS was 1.3, 1.6, 1.6 and 3.1 with significant differences in all groups (P< 0.05). There was non- significant difference in mean age, weight, height and gestational age between all groups (P> 0.05). There was a non- significant difference in APGAR 1, 5, umbilical pH immediately after delivery, neonatal HR after 5minute, 15 minutes and 30 minutes in all groups (P> 0.05). Conclusion: The authors found that dexmedetomidine is a safe and effective adjuvant to intrathecal bupivacaine-dexamethasone in multiparous women undergoing normal vaginal delivery.

scholarly journals Effects of nano mineral-supplemented diet on sow productivity

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Hoang Nghia Son
Keyword(s):  
Birth Weight ◽  
Reproductive Performance ◽  
Metal Content ◽  
Mineral Content ◽  
Control Group ◽  
Average Weight ◽  
Group Iii ◽  
Newborn Piglets ◽  
Group I ◽  
Significant Difference

This study aimed to evaluate the effects of nano mineral-supplemented diet on sow productivity. The data were estimated by several parameters consisting of piglet live ratio at newborn stage and weaning stage, piglet weight at newborn stage and weaning stage, metal content in piglet livers. The results showed that live ratio of newborn piglets in group III was highest (93.44 ± 2.83%) comparing with other groups. However, there was no significant difference of live ratio in piglets at weaning stage between nano mineral-supplemented groups with control. The birth weight of piglets in nano mineral-supplemented groups was similar to control group. The birth weight of piglets in nano mineral-supplemented groups was ranged from 1,361.61 ± 47.37 grams (group II) to 1,410.48 ± 44.28 grams (group I). However, the average weight of weaned pigs in groups III and IV was higher than other groups (6,772.92 ± 105.47 grams and 6,818.87 ± 89.59 grams). Moreover, the amount of Fe, Cu, Zn, Mn in piglet liver from nano mineral-supplemented groups have been found to be lower than those in control group. These results revealed that the supplement of nano minerals for sows promoted the reproductive performance of sows and reduced mineral content in the liver of piglets. 

Association between obesity and the aggravation of limited range of ankle mobility in chronic venous disease

2021 ◽  
pp. 026835552110553
Author(s):  
Sergio Q Belczak ◽  
Rubiana Neves Ramos ◽  
Jose Maria Pereira de Godoy
Keyword(s):  
Varicose Veins ◽  
Limited Range ◽  
Lower Limbs ◽  
Chronic Venous Disease ◽  
Venous Disease ◽  
Limited Joint Mobility ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Ankle Mobility

Aim The aim of the present study was to show that an increase in weight leads to the aggravation of limited joint mobility. Method One hundred sixteen patients with varicose veins of the lower limbs and body mass index (BMI) higher than 30 kg/m2 were evaluated at the Belczak Clinic in Maringá, Brazil. All patients were evaluated by the same physician, and the goniometric readings were performed on all patients by a physiotherapist. The participants were then divided into three groups based on BMI: Group I—BMI between 30 and 40. Group II—BMI between 40 and 50, and Group III—BMI > 50. Results There is a significant difference between the groups detected. Conclusion The present findings show that an increased BMI in obese individuals with chronic venous disease is associated with a progressive limitation of ankle mobility.

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