Elevated Vanin1 and Advillin Expression Is Associated with Progression to Chronic ITP in Children

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 397-397
Author(s):  
Bing Zhang ◽  
Ruchira Sood ◽  
Carol Jones ◽  
Wendy Wong ◽  
Michael Jeng ◽  
...  
Keyword(s):  
T Cell ◽  
Real Time ◽  
Acute Phase ◽  
Real Time Pcr ◽  
Gene Expression Pattern ◽  
Control Group ◽  
Data Set ◽  
Chronic Itp ◽  
Macrophage Phagocytosis ◽  
Acute Itp

Abstract The majority of pediatric ITP patients recover spontaneously within 6 months (acute ITP), while about 20% of the patients develop chronic ITP. The ability to distinguish patients at higher risk of developing chronic disease during the acute phase of the illness could give prognostic information and serve as a basis for clinical trials to study earlier or more aggressive interventions to prevent morbidity associated with long-term immunosuppression. We have previously described the gene expression pattern of pediatric ITP patients. This data set was used to search for genes which would predict progression to chronic ITP. We identified 8 samples from patients with acute self-limited ITP and 4 samples from patients who progressed to chronic ITP, all tested during the acute phase of the disease. At a false discovery rate of 0, two genes—VNN1 (vanin1) and AVIL (advillin) were the most significantly different between the groups. To validate these findings from microarray analysis, real-time PCR experiments were performed using unamplified whole blood RNA from the original cohort, with 4 additional patients (for a total of 9 samples in self-limited acute ITP group and 7 samples in progressed to chronic ITP group), and a control group of 5 normal children. All real-time data was normalized to housekeeping gene (GADPH) expression. Both VNN1 and AVIL expression were significantly increased in the group which developed chronic ITP compared to patients with acute self-limited ITP (VNN1 p=0.008, AVIL p=0.006) and normal controls (VNN1 p=0.044, AVIL p=0.009). Table 1 shows the medians and ranges of the normalized real-time PCR results of the three groups. The mechanism of these over-expressed genes in pediatric ITP patients who progress to chronic ITP is currently being investigated. VNN1 is a GPI anchored protein involved in T cell trafficking and has a pro-inflammatory role as an oxidative-stress response gene. AVIL, whose gene product is a member of the gelsolin/villin family of actin regulatory proteins, is involved in monocyte/macrophage phagocytosis and cytoskeletal remodeling. Thus both of these genes have functions consistent with recent reports implicating T cell regulation and trafficking as well as immune-mediated destruction of platelets in chronic ITP. These findings represent the first candidate biomarkers in predicting prognosis in pediatric ITP and at the same time open the door for further investigation of the molecular mechanism underlying the clinical transition from acute to chronic ITP. A prospective study to validate these findings is in progress. Table1: Normalized VNN1 (Vanin1) Normalized AVIL (Advillin) Median Range Median Range Self-limited Acute ITP group 0.461 0.170~0.813 8.535 0.953~17.395 Progress to chronic ITP group 1.827 0.326~4.723 25.601 6.031~44.296 Normal control group 0.350 0.202~1.029 5.491 1.449~8.585

scholarly journals Relative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populations

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Sebastian Ochsenreither ◽  
Alberto Fusi ◽  
Antonia Busse ◽  
Dirk Nagorsen ◽  
David Schrama ◽  
...  
Keyword(s):  
T Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Cell Populations ◽  
Relative Quantification

scholarly journals The association of male infertility with telomere length: A case control study

2021 ◽  
Vol 8 (4) ◽  
pp. 325-332
Author(s):  
Kate Deepali Rajesh ◽  
Puranam Vatsalaswamy ◽  
Manvikar Purshotam Rao
Keyword(s):  
Male Infertility ◽  
Telomere Length ◽  
Real Time ◽  
Real Time Pcr ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Significant Difference ◽  
Control Study ◽  
Sperm Telomere Length

To study the relevance of sperm telomere length and infertility in men. : Our case-control study included twenty-five males in couple with sub-fertility/infertility (test group) and twenty five healthy males (control group) with proven paternity in the age group 25 to 35 years. The Absolute Sperm Telomere length (aSTL) was measured by real-time PCR. We investigated whether any significant difference in the aSTL value existed between the groups and analysed the relationship between aSTL and other sperm parameters.The mean (SE) aSTL recorded in the infertile cases was significantly shorter than for the control group being 140.60 (6.66) Kb/genome and 239.63 (12.32) Kb/genome respectively (p <0.001) A weak correlation was eminent between aSTL kb/genome and the total sperm count mil/ml (rho= 0.04, p - 0.86), progressive sperm motility (rho= - 0.02, p=0.934) and sperm viability (rho= - 0.07 p=0.741) in the infertile group. The measurement of aSTL by real-time PCR is a simple and rapid method that offers further paramount information with respective to the quality of sperm. It is befitted for epidemiological studies, hence opening new perspectives in the evaluation of male infertility. Limitations - Our study was confined to men aged between 25 and 35 years. Further comparative studies are needed to explore the significance of STL and infertility in older males. Additional studies will help illumine the significance of aSTL as a prognostic biomarker in assisted reproduction.

scholarly journals Astragaloside positively regulated osteogenic differentiation of pre-osteoblast MC3T3-E1 through PI3K/Akt signaling pathway

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Wei Bing Jing ◽  
Hongjuan Ji ◽  
Rui Jiang ◽  
Jinlong Wang
Keyword(s):  
Western Blot ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Signaling Pathway ◽  
Real Time Pcr ◽  
Akt Signaling ◽  
Calcium Deposition ◽  
Control Group ◽  
Akt Signaling Pathway ◽  
Western Blot Assay

Abstract Background Osteoporosis is a widespread chronic disease characterized by low bone density. There is currently no gold standard treatment for osteoporosis. The aim of this study was to explore the role and mechanism of Astragaloside on osteogenic differentiation of MC3T3-E1 cells. Methods MC3T3-E1 cells were divided into control and different dose of Astragaloside (10, 20, 40, 50, and 60 μg/ml). Then, ALP and ARS staining were performed to identify the effects of Astragaloside for early and late osteogenic capacity of MC3T3-E1 cells, respectively. Real-time PCR and western blot were performed to assess the ALP, OCN, and OSX expression. PI3K/Akt signaling pathway molecules were then assessed by Western blot. Finally, PI3K inhibitor, LY294002, was implemented to assess the mechanism of Astragaloside in promoting osteogenic differentiation of MC3T3-E1 cells. Results Astragaloside significantly increased the cell viability than the control group. Moreover, Astragaloside enhanced the ALP activity and calcium deposition than the control groups. Compared with the control group, Astragaloside increased the ALP, OCN, and OSX expression in a dose-response manner. Western blot assay further confirmed the real-time PCR results. Astragaloside could significantly increase the p-PI3K and p-Akt expression than the control group. LY294002 partially reversed the promotion effects of Astragaloside on osteogenic differentiation of MC3T3-E1 cells. LY294002 partially reversed the promotion effects of Astragaloside on ALP, OCN, and OSX of MC3T3-E1 cells. Conclusion The present study suggested that Astragaloside promoted osteogenic differentiation of MC3T3-E1 cells through regulating PI3K/Akt signaling pathway.

scholarly journals CHANGES IN GENE EXPRESSION ASSOCIATED WITH NON-CANCER EFFECTS OF THE CHORNOBYL CLEAN-UP WORKERS IN THE REMOTE PERIOD AFTER EXPOSURE

2020 ◽  
Vol 25 ◽  
pp. 456-477
Author(s):  
I. Ilienko ◽  
◽  
D. Bazyka ◽  
N. Golyarnyk ◽  
L. Zvarych ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Main Group ◽  
Control Group ◽  
Chronic Obstructive ◽  
Relative Level ◽  
Gstm1 Gene ◽  
Expression Of Genes ◽  
Immune Inflammation

Objective. to establish the connection of radiation-induced changes in gene expression with the realized pathology of the broncho-pulmonary and cardiovascular systems in Chornobyl clean-up workers. Materials and methods. We examined 314 male Chornobyl clean-up workers (main group; age (58.94 ± 6.82) years (M ± SD); min 33, max 79 years; radiation dose (411.82 ± 625.41) mSv (M ± SD); min 1.74, max 3600 mSv) with various nosological forms of cardiovascular and broncho-pulmonary pathology (BPP) and 50 subjects of the control group: age (50.50 ± 5.73) years (M ± SD); min 41, max 67 years. The relative level of BCL2, CDKN2A, CLSTN2, GSTM1, IFNG, IL1B, MCF2L, SERPINB9, STAT3, TERF1, TERF2, TERT, TNF, TP53, CCND1, CSF2, VEGFA genes expression was determined in peripheral blood leukocytes by real-time PCR (7900 HT Fast Real-Time PCR System (Applied Biosystems, USA)). The «gene-disease» association was determined on statistical models stratified separately for each disease and gene. Logistic regression was used to calculate the odds ratio. Results. Increased GSTM1 gene expression and no changes in angiogenesis-related VEGFA gene expression were found in the main group of patients with coronary heart disease (CHD). It was established overexpression of TP53, VEGF and IFNG genes in the group of patients with arterial hypertension (AH). At combination of these diseases an increase of expression of СSF2, TERF1, TERF2 genes was established. The detected changes demonstrate an activation of the antioxidative defense system in patients with CHD, while AH is associated with the expression of genes of angiogenesis and immune inflammation. It was shown an increase in the expression of genes associated with apoptosis and kinase activity (BCL2, CLSTN2, CDKN2), immune inflammation (CSF2, IL1B, TNF) in Chornobyl clean-up workers with BPP. Expression of TP53 and GSTM1 (gene, associated with the glutathione system) was significantly upregulated in the group of individuals with chronic bronchitis, whereas in patients with chronic obstructive pulmonary disease, no increase was detected; the expression of SERPINB9 and MCF2L genes was downregulated. Conclusions. Changes in the expression of genes, associated with the development of somatic pathology in the remote period after irradiation, in particular the genes of the immune response and inflammatory reactions CSF2, IFNG, IL1B, TNF; expression of genes that regulate cell proliferation, aging and apoptosis TP53, BCL2, MCF2L, CDKN2A, SERPINB9, TERF1, TERF2, TERT; genes that regulate cell adhesion and angiogenesis CLSTN2, VEGF. Key words: gene expression, somatic pathology, radiation, Chornobyl.

4 MYOSTATIN GENE KNOCKDOWN THROUGH LENTIVIRAL VECTOR-MEDIATED DELIVERY OF shRNA FOR IN VITRO PRODUCTION OF TRANSGENIC BOVINE EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 120 ◽  
Author(s):  
M. P. Milazzotto ◽  
W. B. Feitosa ◽  
B. E. Strauss ◽  
M. Bajgelman ◽  
C. M. Mendes ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Morphology ◽  
Real Time Pcr ◽  
Lentiviral Vector ◽  
Gene Knockdown ◽  
Control Group ◽  
Bovine Embryos ◽  
Myostatin Gene ◽  
Transgenic Embryo

The main goal of husbandry and beef cattle production is to enhance performance rates, for example, weight gain. Myostatin is referred to as a negative regulator of skeletal muscle growth. Genetic engineering of this character in order to produce double muscling animals that can transmit to future progeny will enhance its usefulness. The present research aimed to analyze myostatin inhibition through lentiviral-mediated delivery of shRNA in mouse myoblast culture and the feasibility of the lentiviral-mediated delivery of shRNA into in vitro-produced transgenic bovine embryos. In order to achieve knockdown of myostatin in cell and embryo culture, a lentiviral vector was constructed with ubiquitin C promoter-driven GFP gene (green fluorescent protein) and shRNA to suppress myostatin gene expression driven by the U6 promoter. Vector efficiency was verified through in vitro murine myoblast (C2C12) cell morphology after inductive differentiation and by means of real-time PCR of myostatin and GAPDH genes. Later, bovine oocytes were in vitro-matured and the lentiviral vector was microinjected into the oocyte perivitelline space (2.5 � 106 IU mL-1) after mechanical and chemical cumulus cell removal. Non-microinjected mature oocytes were considered as control. After microinjection, oocytes were fertilized and cultured in vitro. After 4 and 9 days of culture, embryos were evaluated by epifluorescence microscopy. The GFP-positive embryos were green under fluorescence. Cell morphology and embryo development rate data were analyzed by Minitab Release 14 Statistical Software (Minitab, Inc., State College, PA, USA), submitted to ANOVA, and compared by Tukey test (P d 0.05). Real-time PCR data were analyzed by Pair-Wise Fixed Reallocation Randomization Test using REST2005 software. Cell morphology results demonstrated that the vector was able to inhibit myostatin mRNA in C2C12 cells as the transducted group progressed less to myotubes than in the control group. A lower amount of myostatin mRNA after 72 h of differentiation indicated an inhibition tendency by real-time PCR. In relation to the transgenic embryo production, 96.9 � 0.34% (62.65) developed to cleavage, 80.24 � 4.38% (51/65) were GFP-positive, and 50.95 � 3.37% (26/65) achieved blastocyst stage. After hatching, 3.07% (2/65) of GFP-positive embryos maintained fluorescence. In relation to the control group, the cleavage rate was 93.81 � 0.68% (61/65); the blastocyst rate 38.34 � 2.36% (25/65), and none were fluorescent. In conclusion, myostatin gene knockdown was effectively performed by lentiviral vector-mediated delivery of shRNA. Thus, novel studies about the efficiency of this vector on transgenic embryo production can be performed. This work was supported financially by FAPESP 03/0156-9.

The Characteristics of T Lymphocytic Clones Correlate with the Pathogenesis of Idiopathic Thrombocytopenic Purpura.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1252-1252
Author(s):  
Ping Zhu ◽  
Xia Zhu ◽  
Xiao-ling Guo ◽  
Jiang-ying Gu ◽  
Yuan Ou
Keyword(s):  
T Cell ◽  
Size Distribution ◽  
Idiopathic Thrombocytopenic Purpura ◽  
Healthy Controls ◽  
Thrombocytopenic Purpura ◽  
T Cell Clones ◽  
Average Value ◽  
Chronic Itp ◽  
Cell Clones ◽  
Acute Itp

Abstract Objective To determine the characteristics of T lymphocytic clones that correlate with the pathogenesis of idiopathic thrombocytopenic purpura (ITP) by investigating complementarity determining region (CDR3) repertoires of T cell receptors (TCRs) β chain variable region (BV). Methods Twenty-five of patients with idiopathic thrombocytopenic purpura (including 15 patients with acute ITP and 10 patients with chronic ITP), twenty of normal peoples and 20 of normal umbilical cord blood samples were enrolled. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify 24 subfamily genes of TCR BV from peripheral blood lymphocytes of ITP patients and normal controls, the PCR products were run on denatured polyacrylamide sequencing gel, establishing spectratyping of TCR BV CDR3 gene expressing repertoire. The bands that dense or disappeared of TCR BV gene repertoire on the electrophoresis gel were used to analyze the variety of T cell clones in cases of ITP. The dense bands in the gel were cut down and sequencing. Compare with those major sequences of TCRBV gene in normal and abnormal T cell clones to understand the relationship among the TCRBV gene and ITP. Results: In acute ITP (aITP), the spectratyping of TCR BV CDR3 size distribution were similar to normal control (P=0.179), the oligoclonality could be observed with an average value of 2.73±0.88 per person in 15 cases of aITP. TCR BV CDR3 size distribution had little difference compared with aITP and healthy controls. Abnormal spectratyping of TCR BV CDR3 distribution could be observed significantly different between chronic ITP (cITP) patients and healthy controls (P<0.05), with oligoclonality at an average value of 7.2±3.04 per person in 10 cases of cITP. Same T cell clone expansions could be observed in gene subfamilies of TCR BV8,BV13.1,BV14,BV17. In 10 of cITP patients, sequences could be read from 19 out of 20 dense bands on the gene spectratyping of TCR BV CDR3, which suggested some dominant T cell clones expanded in cITPs. In different patients of cITP, the expanded T cell clones shared identical or similar TCR BV gene or CDR3 encoded by the TCR BV gene. Two T cell clones in 2 patients had identical TCR BV8 gene sequences separately. Other 2 T cell clones in another 2 cITP patients had same TCR BV13.1. It showed those expanded T cell clones in the bodies of the cITP patients could recognize common antigen. The similar CDR3 was found in 2/4 expanded TCR BV17 T cell clones, with only 2 amino acids different in framework 4 (FR4). Two T cell clones had almost same sequence of CDR3 in TCR BV17 except for 4 basepairs difference in their full sequences. In analyzing the motifs in CDR3 of different cITPs, we found three common motifs (E/DTQYFGPG;N(K)EQFFGPG;GANVLTFGAG) which were separately used in different TCR BV CDR3 of 19 expanded T cell clones. Among these motifs, TCR BV of 7/19 T cell clones shared common E/DTQYFGPG;TCR BV of 4/19 T cell clones shared common N(K)EQFFGPG; TCR BV17 of 3/4 T cell clones had common GANVLTFGAG. Compared with the various sequence of 10 T cell clones in 6 cases of SLE patients that we had reported (Journal of Chinese Medicine2003;83(10):1648–1652), 5/10 T cell clones of the SLEs shared the motif NEQFFGPG in the CDR3.Three of TCR BV13.1 in these T cell clones showed motif DTQYFGPG. Conclusion the cITPs have some abnormal expanded T cell clones that correlate with the pathogenesis, while aITPs have no significant expanded clones. All the cITP patients share 3 common motifs in TCR BV CDR3, which is possibly to recognize similar autoantigens.

Interferon Gamma +874A/T Polymorphism in Children with Idiopathic Thrombocytopenic Purpura

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4539-4539
Author(s):  
Fatih Demircioglu ◽  
Hale Ören ◽  
Sefa Kizildag ◽  
Sebnem Yilmaz ◽  
Berna Atabay ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Interferon Gamma ◽  
Gene Polymorphisms ◽  
Statistical Difference ◽  
Control Group ◽  
Blood Samples ◽  
Chronic Itp ◽  
Significant Difference ◽  
Acute Itp ◽  
And Control

Abstract A recent study showed that expression of Toll-like receptor and interferon-gamma associated genes is significantly increased in patients with chronic ITP. Interferon-gamma is an important protein which takes place in immunoregulation. +874A/T polymorphism in the first introne of interferon gamma gene is found to be associated with the development and clinical phenotype of some autoimmune diseases such as diabetes mellitus, thyroiditis, multiple sclerosis, and SLE. The aim of our study was to investigate whether interferon gamma +874A/T polymorphism is a risk factor for the development of ITP and whether it affects the clinical course and response to the treatment. Thirty five children with acute ITP and 40 children with chronic ITP who were followed for at least 6 months were included. Control group consisted 90 healthy children. Two millilitres of blood sample was taken into sterile tubes containing 0.1% EDTA from each child and all blood samples were stored at −20 until analysis. DNA was isolated from blood samples and interferon gamma +874A/T polymorphism was studied with real-time PCR and LightCycler TM. Twenty one patients had AA, 35 patients had AT, and 19 patients had TT genotype. In the control group, 47 children had AA, 36 children had AT, and 7 children had TT genotype. There was a statistical difference between ITP and control group regarding the genotype (p=0.001). The frequency of A and T alleles in ITP group was 52% and 48%, respectively. The frequency of A and T alleles in control group was 72.7% and 27.8%, respectively. The frequency of allele distribution was statistically different between the ITP and control groups (p<0.0001). There was a statistical significant difference between acute ITP and control group regarding the frequency of AA, AT, and TT gene polymorphisms and allele frequency (p=0.002, p=0.002). Similarly, there was a statistical significant difference between chronic ITP and control group regarding the frequency of AA, AT, and TT gene polymorphisms and allele frequency (p=0.008, p=0.002). The frequency of AA, AT, and TT gene polymorphisms and allele frequency showed no statistical difference between acute and chronic ITP groups (p=0.285, p=0.896). There was no correlation between interferon gamma +874A/T polymorphism and severity of bleeding (mild, moderate and severe) (p=0.09). There was no correlation between interferon gamma +874A/T polymorphism and response to long term treatment in patients with chronic ITP (p=0.568). In conclusion, there was a significant difference between patients with ITP and children in control group regarding interferon gamma +874A/T polymorphism and in the light of recent data involving other autoimmune disorders, we think that interferon gamma +874A/T polymorphism may be a risk factor for ITP.

The Alteration of SEPT5 Gene Expression in BCR-ABL Positive Cells and Its Possible Correlation with the Development and / or Progression of Chronic Myeloid Leukemia (CML)

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4415-4415
Author(s):  
Cintia Do Couto Mascarenhas ◽  
Anderson Ferreira Cunha ◽  
Ana Flavia Brugnerotto ◽  
Sheley Gambero ◽  
Joao Machado-Neto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Cell Lines ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Mononuclear Cells ◽  
Conflicts Of Interest ◽  
Blood Platelets ◽  
Gene Expression Pattern ◽  
P Value

Abstract Abstract 4415 The CML is a clonal disease of stem cells and its main feature is the unregulated production of a tyrosine kinase protein called BCR-ABL, the progression of the disease to accelerated phase or blast crisis may be associated with genomic instability. Because of this, the use of tools for the study of gene expression could bring new insights in the understanding of these mechanisms in the CML. In a recent study using SSH libraries, we compared the gene expression pattern between granulocytes of health control and CML patients, and we identified the gene SEPT5 expressed only in CML patients. Although the studies in the literature, there is not a clear relationship between the expression of this gene and the development or progression of CML. SEPT5 is a member of nucleotide binding proteins called septins that were firstly described in yeast as cell division cycle regulatory proteins. This gene was reported in patients with AML translocated with MLL gene, in adult human brain and heart; it is also associated with alpha granules of human blood platelets. The aims of this study are to carry a functional analysis of SEPT5 in differents cells line and to study the relationship of this gene and the development and/or progression of CML. The gene expression evaluation was made in granulocytes, mononuclear cells and total leukocytes of CML patients and healthy blood donors in peripheral blood. It was also evaluated in bone marrow donors, in human cell lines (K562, HL60 and NB4) and in mice cell lines (BaF3/BCR-ABLp210 and BaF3T315I), performed by real-time PCR for the following genes: SEPT5, β-actin and GAPDH. Experiments were also performed to verify the difference between the chemotaxis of granulocytic cells from controls and patients by ELISA. Data were analysed statistically using the ANOVA followed by Dunnett’s test – P value of less than 0.05 was considered to be significant. The study was approved by the Research Ethic Committee of the Faculty of Medical Sciences of University of Campinas. The gene expression of SEPT5 was evaluated by real time PCR using the same samples used in the library construction to validate the results found in the SSH library. The data confirmed our previous results, showing that the SEPT5 expression is increased in all cells of patients compared to controls. The same results were observed when we studied the expression comparing individually patients and health blood donors, suggesting that this protein could be increased in all human cells that present the translocation BCR-ABL. The level of expression of this gene in HL60 and NB4 was significantly lower than in K562 cell line. The experiments with mice cell lines showed a higher expression of this gene in BaF3T315I when compared to BaF3BCR-ABLp210. We obtained a significant expression difference in all experiments (p <0.05). The spontaneous and stimulated with IL-8 chemotaxis assays used granulocytes and were assessed using chamber containing 96 wells. However, although the results suggest an increased chemotactic activity in patients, there were no significant differences (p<0.05) between controls and patients – regardless of whether the chemotaxis was spontaneous or stimulated with IL-8. In mammals the SEPT5 gene is associated with cellular processes such as exocytosis, apoptosis, leukemogenesis, carcinogenesis and neurodegeneration. Therefore, molecules capable of interacting with the septins, either at biochemical or molecular level, can bring information about their functions in cytokinesis. Studies indicate that the human septins can interact among themselves and with other components of the cytoskeleton – this may be a relevant observation regarding the function of this gene in cancer. The SEPT5 can be activated by different pathways – this may increase expression in translocated cells. Despite major advances in the treatment of CML, the treatments available are not capable of inactivating all the signaling pathways activated by BCR/ABL. Our results demonstrate that SEPT5 may be involved in the pathophysiology of CML. Also, it is clear the importance of the study of pathways that could culminate in its high expression or the triggering of other unknown pathways involved in the development of CML. The increased expression of this gene may be related to disease progression, and finally, the identification of several important genes may lead to a better understanding of CML and helping to identify new therapeutic targets. FAPESP/INCT. Disclosures: No relevant conflicts of interest to declare.

Sign in / Sign up

Export Citation Format

Share Document