Vitamin E Does Not Reduce Cardiomyopathy In Doxorubicin treated Rats

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4944-4944
Author(s):  
Jhon A Guerra ◽  
Maribel Torres-Serrant ◽  
Pedro J Santiago
Keyword(s):  
Vitamin E ◽  
Conflicts Of Interest ◽  
Cardiac Injury ◽  
Free Radical Scavenger ◽  
Alpha Tocopherol ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Radical Scavenger ◽  
Doxorubicin Treatment ◽  
Doxorubicin Group

Abstract Abstract 4944 Introduction: The clinical efficacy of doxorubicin is severely limited by its cardiotoxicity. Antioxidants represent the largest class of chemicals examined as potential protective agents and for which there is continuing interest. Dexrazoxane is the current agent used for the control of doxorubicin related cardiotoxicity. However, in large trials the incidence was reduced only by 50% (1). Vitamin E, a known antioxidant and free radical scavenger agent, has been evaluated in the past as a cardio protective drug in animal models receiving doxorubicin but contradictory conclusions regarding this effect have been reported (2, 3). We hypothesized whether Vitamin E has an effect in cardioprotection in rats receiving doxorubicin. Design and Methods: Three groups of 6 Rats each were used for the experiment. Group 1 received doxorubicin 1.5mg/kg intraperitoneally (IP) weekly for 9 weeks for a total cumulative dose of 13.5mg/kg. Group 2 received Vitamin E 100 IU/kg/weekly by IP injection, 48 hours prior to the doxorubicin. Group 3 received 0.5 cc of saline solution 0.9% IP weekly for 9 weeks as control group. Functional parameters including plasmatic nitric oxide (NO) levels and cardiac ejection fraction (EF) were determined on each group at the end of the experiment. Results: Doxorubicin treatment significantly increased plasmatic NO concentration when compared with controls (35.30 ± 5.63 mM vs. 14.72 ± 2.66 mM, n=6, P=0.016); however, in the group of rats receiving Vitamin E prior to doxorubicin, NO did not have a significant decrease (from 35.30 ± 5.63 mM to 31.77± 8.91 mM n=6, P=0.75). Regarding EF, doxorubicin treatment decreased EF significantly when compared with saline controls rats (59 ± 5.61% vs. 77 ± 3.89 %, n=6, P<0.05); however, in the group of rats receiving Vitamin E prior to doxorubicin, EF did not have a significant improvement (from 59 ± 5.61% to 69.17 ± 4.4, n=6, P=0.24). Conclusion: These results suggest that Vitamin E does not prevent or reduce cardiac injury in rats treated with doxorubicin. Different alternatives including other antioxidants could be explored in an attempt to decrease cardiotoxicity produced by doxorubicin and to improve the effect of the standard cardioprotective agent used Dexrazoxane. Further studies are necessary. References: 1. Swain SM. Adult multicenter trials using dexrazoxane to protect against cardiac toxicity. Semin Onco 1998; 25 (4 Suppl 10):43-7 2. Breed JG, Zimmerman AN, Dormans JA, Pinedo HM. Failure of the antioxidant vitamin E to protect against adriamycin-induced cardiotoxicity in the rabbit. Cancer Res 1980; 40:2033-8 3. Myers CE, McGuire W, Young R. Adriamycin: amelioration of toxicity by alpha-tocopherol. Cancer Treat Rep 1976; 60:961-2 Disclosures: No relevant conflicts of interest to declare.

Protective effect of vitamin E and epicatechin against nicotine-induced oxidative stress in rats

2012 ◽  
Vol 29 (2) ◽  
pp. 202-208 ◽  
Author(s):  
Abdulrahman L Al-Malki ◽  
Said S Moselhy
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Combined Treatment ◽  
Antioxidant Properties ◽  
Free Radical Scavenger ◽  
Individual Treatment ◽  
Antioxidant Vitamin ◽  
Radical Scavenger ◽  
Pharmacologically Active ◽  
Chemopreventive Activity

Nicotine is a major pharmacologically active and addictive component of tobacco smoke, which is regarded to be a primary risk factor in the development of cardiovascular and pulmonary diseases. Epicatechin is one of the most potent antioxidants present in the human diet. Particularly high levels of this compound are found in tea, apples and chocolate. It has been reported that tea extracts and/or its constituents have antibacterial, antiviral, antioxidative, antitumor and antimutagenic activities. Vitamin E is a major lipid-soluble antioxidant vitamin and free radical scavenger, presents as an integral component of cellular membranes and has important biological functions. The primary mechanism by which vitamin E is proposed to prevent cancer is through their antioxidant properties. The goal of this study is to evaluate the effect of epicatechin alone or combined with vitamin E in inhibiting the oxidative stress induced by nicotine in rats. Results obtained indicated that there was a significant elevation in the levels of malondialdhyde (MDA) in nicotine injected rats. The combined treatment (epicatechin + Vit E) group showed a potential reduction of these parameters more than individual treatment. The activities of superoxide dismutase, catalase and glutathione peroxidase were found significantly higher in combined treated than untreated rats. In nicotine group, a negative significant correlation between reduced glutathione and MDA ( r = −0.92) was observed. In conclusion, these results suggested that the supplementation of diet with epicatechin and vitamin E provided antioxidant defense with strong chemopreventive activity against nicotine-induced carcinogenesis.

scholarly journals Comparison of the Antioxidant Nutrient Profile among Normal Pregnant Women and Women having Pregnancy Induced Hypertension

2021 ◽  
Vol 10 (4) ◽  
pp. 246-251
Author(s):  
Kausar Aamir ◽  
Arfa Azhar ◽  
Fatima Abid ◽  
Shamaila Khalid ◽  
Fiza Ali Khan
Keyword(s):  
Vitamin E ◽  
Pregnant Women ◽  
Cell Damage ◽  
Alpha Tocopherol ◽  
Control Group ◽  
P Value ◽  
Pregnancy Induced Hypertension ◽  
Nutrient Profile ◽  
Induced Hypertension ◽  
Mild Hypertensive

Background: Preeclampsia is a multifactorial disorder comprising many organs. Oxidative stress (OS) has been intensely linked to its occurrence. Vitamin E, a lipophilic chain breaking antioxidant has been proved to suppress the OS. Present study was designed to investigate antioxidant nutrient profile in patients with different grades of pregnancy induced hypertension (PIH) and to compare them with normal pregnant controls. Methods: The study group comprised 110 patients divided in three groups as Group A (n=40) Normotensive patients, Group B (n=40) Mild hypertensive, Group C (n=30) Severe hypertensive. Vitamin A, B-Carotene, serum alpha tocopherol (vitamin E) and vitamin C levels were analysed. Results: Serum alpha tocopherol (vitamin E) was significantly low in severe and mild cases (0.32±0.00 mg/dl, 0.74±0.03 mg/dl respectively), when compared with normal pregnant women levels (0.78±0.040). All other nutrients were also found to be in reduced quantity for Group C when compared to control group (P value <0.001). Conclusion: It was therefore concluded that in patients with risk of preeclampsia (PE) adequate antioxidant nutrients may have a role in cessation of free radical-mediated cell disturbances, and thereby protecting against endothelial cell damage, which is the key factor in PE development.

scholarly journals Efficacy of Ashwagandha (withania somnifera [l.] Dunal) in improving cardiorespiratory endurance (VO2 max test) in healthy subjects

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Bargale Sushant Sukumar ◽  
Tripathy T B ◽  
Shashirekha H K ◽  
Suhas Kumar Shetty
Keyword(s):  
Healthy Subjects ◽  
Withania Somnifera ◽  
Nutritional Supplement ◽  
Free Radical Scavenger ◽  
Control Group ◽  
Radical Scavenger ◽  
Study Group ◽  
Oxygen Intake ◽  
Vo2 Max ◽  
Cardiorespiratory Endurance

In Ayurveda, certain herbal formulas are considered to be Rasayana and they are typically taken over periods of time to regenerate both brain and body tissue. Ashwagandha (Withania Somnifera) is used as an adaptogen, antioxidant, immune modulator, free radical scavenger, anti stress, anti arthritic, antispasmodic, anti inflammatory, nervous tonic, nerve soothing and anticancer agent. Ashwagandha (WS) as a nutritional supplement is yet too established. Maximum oxygen uptake (VO2 max) is a gold standard of cardiopulmonary and muscle cell fitness is considered.  The study evaluated the efficacy of Ashwagandha to improve cardiorespiratory endurance (VO2 max) in healthy subjects. They randomized single blind controlled comparative clinical study. 54 health volunteers in each group, study group received Ashwagandha Choorna 12gm with milk (200ml) empty stomach in the morning and the control group only milk (200ml). Maximal capacity of oxygen intake in ml/kg/min (VO2 max) with Rockport fitness walking test of both study and control group were measured before intervention (0th day), after the intervention (60th day) and follow up (90th day). A significant improvement in the VO2 max (F=20.675, P <0.0001) and Hemoglobin (X2=74.150 P <0.0001) in the study group was found. Supplementation of Ashwagandha (Withania Somnifera) with milk improve hemoglobin and VO2 max (maximum aerobic capacity).

scholarly journals Cellular Damage After Prolonged Low-Dose Exposure of Neonicotinoid in Rats

2021 ◽  
Author(s):  
Rahat Naseer ◽  
Affan Tariq ◽  
Munazza Raza Mirza ◽  
Muhammad Rashid ◽  
Syed Qasim Raza ◽  
...  
Keyword(s):  
Vitamin E ◽  
Low Dose ◽  
Prolonged Exposure ◽  
Cell Damage ◽  
Hematological Parameters ◽  
Treated Group ◽  
Alpha Tocopherol ◽  
Cellular Damage ◽  
Control Group ◽  
New Class

Abstract Background; Dinotefuran is a new class of neonicotinoids claimed to be harmless to mammals and humans. This claim was daunted by the documented effect of dinotefuran on honeybees and further studies were required. Aim: The study was designed to assess the capaciousness of damage caused by prolonged exposure of dinotefuran in mammals and probable strategy to neutralize its effect. Methodology: Ninety-day trial using Wistar rats (n=45) was conducted while dividing them into three groups: untreated control group, insecticide (dinotefuran) treated group, and dinotefuran treated and vitamin E supplemented group. Dinotefuran was administrated orally (LD25). Vitamin E (alpha-tocopherol) supplementation was given in water ad libitum. Blood sampling was done twice a month, and hematological and biochemical data were recorded. After expiry of trial period, the experimental rats were anesthetized and sacrificed. Organs (kidneys, liver, and heart) were isolated from each groups, weighed, and stored at approximately -20°C till further processing, analysis and histopathology were performed. Results: All the hematological parameters were affected significantly. Histopathology of tissues showed clear necrosis in all the tissues except kidneys. All the biomarkers of oxidative stress and comet assay demonstrated significant cell damage. All the parameters showed improvement after vitamin E supplementation but non-significantly. Significance: These findings were suggestive that even low dose persistent exposure can lead to mutagenicity and carcinogenicity in mammals and other non-target species hence revised policy guidelines and more intelligent use of these chemicals is required.

S109 – N-2 MPG in Groin Flaps Subjected to Vein Occlusion

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P113-P114
Author(s):  
Benoit J Gosselin ◽  
Louise Davies
Keyword(s):  
Free Radical ◽  
Sham Group ◽  
Venous Occlusion ◽  
Free Radical Scavenger ◽  
Control Group ◽  
Radical Scavenger ◽  
Flap Survival ◽  
Vein Occlusion ◽  
Scavenger Activity ◽  
The Mean

Objectives In a prior communication, we showed that ischemic rat groin flaps exposed to the free radical scavenger N-2 mercaptopropionylglycine (MPG) at the time of arterial and venous occlusion had a higher rate of survival than untreated flaps. In this study, the objective is to test the efficacy and timing of administration of MPG in salvaging rat groin flaps subjected to venous occlusion alone. Methods Randomized controlled trial. Main outcome is mean percentage of flap survival at 7 days. 30 mature Sprague-Dawley rats were randomized to 3 groups based on timing of treatment with MPG: 1- flap raised, no MPG, no venous occlusion (sham group); 2- flap raised, no MPG, 10-hour period of venous occlusion (control group); 3- flap raised, MPG after 10 hours venous occlusion (post-reperfusion group). Results There was no statistical difference noted in mean survival of venous occluded flaps when comparing the control and post-reperfusion groups, after 7 days. Specifically, the mean flap survival in the control group at 7 days was 10.9% (median 0%, range 0–100%). The mean flap survival in the post-reperfusion group was 14.6% (median 0%, range 0–100%). The mean flap survival in the sham group was 90.0% (median 100%, range 0–100%). Conclusions Post-reperfusion MPG administration is not helpful for flaps subjected to venous occlusion alone. The free-radical scavenger activity of MPG is potentiated within the tissues prior to vein occlusion. Potential applications for free flap salvage would need further study.

Abstract TP447: Edaravone Reduces Hyperperfusion-related Neurological Deficits After Direct Bypass in Adult Moyamoya Disease

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Haruto Uchino ◽  
Naoki Nakayama ◽  
Ken Kazumata ◽  
Kiyohiro Houkin
Keyword(s):  
Moyamoya Disease ◽  
Free Radical Scavenger ◽  
Adult Patients ◽  
Neurological Deficits ◽  
Control Group ◽  
Radical Scavenger ◽  
Direct Bypass ◽  
Adult Moyamoya Disease ◽  
And Control ◽  
Serial Measurements

Background and Purpose: Postoperative hyperperfusion-related transient neurological deficits (TNDs) are frequently observed in adult patients with moyamoya disease (MMD) who undergo direct bypass procedures. The present study evaluated the effect of the free radical scavenger edaravone on postoperative hyperperfusion in adult MMD. Methods: This study included 92 hemispheres in 72 adult patients who underwent direct bypass for MMD. Serial measurements of cerebral blood flow were conducted immediately after surgery and on postoperative days 2 and 7. In 40 hemispheres in 36 patients, edaravone (60 mg/day) was administered from the day of surgery until postsurgical day 7. The incidence of postoperative hyperperfusion and associated TNDs were compared with a control group that included 52 hemispheres in 36 patients. Results: Radiological hyperperfusion was observed in 28/40 (70.0%) and 39/52 (75.0%) hemispheres in the edaravone and control groups, respectively (P = 0.30). Hyperperfusion-related TNDs incidences were significantly lower in the edaravone group compared with the control group (12.5% vs. 32.7%, P = 0.024). Multivariate analysis demonstrated that edaravone administration (P = 0.009) and left-sided surgery (P = 0.037) were significantly correlated with hyperperfusion-related TNDs (odds ratios, 0.3 and 4.2, respectively). Conclusions: Perioperative administration of edaravone reduced the incidence of hyperperfusion-related TNDs after direct bypass procedures in adult patients with MMD.

Protective effect of edaravone in inner-ear barotrauma in guinea pigs

2006 ◽  
Vol 120 (7) ◽  
pp. 524-527 ◽  
Author(s):  
Y Masuda ◽  
T Tanabe ◽  
Y Murata ◽  
S Kitahara
Keyword(s):  
Protective Effect ◽  
Inner Ear ◽  
Guinea Pigs ◽  
Auditory Brainstem ◽  
Free Radical Scavenger ◽  
Control Group ◽  
Radical Scavenger ◽  
Threshold Elevation ◽  
Brainstem Response ◽  
Inner Ear Barotrauma

Objective: The purpose of this study was to determine the protective effect of edaravone, a free radical scavenger, on inner-ear barotrauma (IEB) in guinea pigs, based on a hypothesis implicating free radicals in the development of IEB.Materials and methods: One hundred and twenty-five guinea pigs were divided into a control group and a pretreatment group. After auditory brainstem response (ABR) testing, the pretreatment group received 9.0 mg/kg intraperitoneal edaravone. Animals were exposed to pressure loading and then to further ABR testing.Results: The incidence of IEB was 62.7 per cent in the control group and 42.9 per cent in the pretreatment group (p < 0.01). The distributions of threshold elevation in the control group were 37.3 per cent (for 10 dB or less), 21.3 per cent (for 20–30 dB), 18.0 per cent (for 40–60 dB) and 23.4 per cent (for 70 dB or more), and those in the pretreatment group were 57.1 per cent, 19.1 per cent, 14.3 per cent and 9.5 per cent, for the same respective decibel levels (p < 0.01).Conclusions: These results suggest that protective treatment with edaravone can significantly reduce both the incidence of IEB and the severity of the resultant ABR threshold elevation.

scholarly journals Effects of Edaravone, a Free Radical Scavenger, on Photochemically Induced Cerebral Infarction in a Rat Hemiplegic Model

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Satoshi Ikeda ◽  
Katsuhiro Harada ◽  
Akihiko Ohwatashi ◽  
Yurie Kamikawa
Keyword(s):  
Free Radical ◽  
Cerebral Infarction ◽  
Rose Bengal ◽  
Green Light ◽  
Recovery Process ◽  
Treated Group ◽  
Free Radical Scavenger ◽  
Control Group ◽  
Radical Scavenger ◽  
Cerebral Thrombosis

Edaravone is a free radical scavenger that protects the adjacent cortex during cerebral infarction. We created a hemiparetic model of cerebral thrombosis from a photochemically induced infarction with the photosensitive dye, rose bengal, in rats. We examined the effects of edaravone on recovery in the model. A total of 36 adult Wistar rats were used. The right sensorimotor area was irradiated with green light with a wavelength of 533 nm (10 mm diameter), and the rose bengal was injected intravenously to create an infarction. The edaravone group was injected intraperitoneally with edaravone (3 mg/kg), and the control group was injected with saline. The recovery process of the hemiplegia was evaluated with the 7-step scale of Fenny. The infarcted areas were measured after fixation. The recovery of the paralysis in the edaravone-treated group was significantly earlier than that in the untreated group. Seven days later, both groups were mostly recovered and had scores of 7, and the infarction region was significantly smaller in the edaravone-treated group. Edaravone reduced the infarction area and promoted the functional recovery of hemiparesis from cerebral thrombosis in a rat model. These findings suggest that edaravone treatment would be effective in clinical patients recovering from cerebral infarction.

scholarly journals DPPH FREE RADICAL SCAVENGER ACTIVITY OF FLAVONOID FROM THE LEAVES OF FERN Chingia sakayensis (Zeiller) Holtt

2010 ◽  
Vol 5 (2) ◽  
pp. 173-176
Author(s):  
Suyatno Suyatno ◽  
Noor Cholies Zaini ◽  
Motoo Tori
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Vitamin E ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Free Radical Scavenger ◽  
Flavonoid Compound ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

A flavonoid compound in flavonol type namely kaemferol was isolated from the ethyl acetate fraction of the methanol extract of the fern Chingia sakayensis (Zeiller) Holtt's leaves. The DPPH free radical scavenger activity of kaemferol was stronger than buthyl hyroxy toluene (BHT) but it was weaker than ascorbic acid (vitamin C) and -tocopherol (vitamin E).   Keywords: Chingia sakayensis, kaemferol, DPPH free radical scavenger activity  

scholarly journals L-Malate Enhances the Gene Expression of Carried Proteins and Antioxidant Enzymes in Liver of Aged Rats

2015 ◽  
pp. 71-78 ◽  
Author(s):  
X. ZENG ◽  
J. WU ◽  
Q. WU ◽  
J. ZHANG
Keyword(s):  
Gene Expression ◽  
Antioxidant Enzymes ◽  
Free Radical Scavenger ◽  
Control Group ◽  
Radical Scavenger ◽  
Aged Rats ◽  
Inner Mitochondrial Membrane ◽  
Expression Levels ◽  
Antioxidant Mechanism ◽  
And Control

Previous studies in our laboratory reported L-malate as a free radical scavenger in aged rats. To investigate the antioxidant mechanism of L-malate in the mitochondria, we analyzed the change in gene expression of two malate-aspartate shuttle (MAS)-related carried proteins (AGC, aspartate/glutamate carrier and OMC, oxoglutarate/malate carrier) in the inner mitochondrial membrane, and three antioxidant enzymes (CAT, SOD, and GSH-Px) in the mitochondria. The changes in gene expression of these proteins and enzymes were examined by real-time RT-PCR in the heart and liver of aged rats treated with L-malate. L-malate was orally administered in rats continuously for 30 days using a feeding atraumatic needle. We found that the gene expression of OMC and GSH-Px mRNA in the liver increased by 39 % and 38 %, respectively, in the 0.630 g/kg L-malate treatment group than that in the control group. The expression levels of SOD mRNA in the liver increased by 39 %, 56 %, and 78 % in the 0.105, 0.210, and 0.630 g/kg L-malate treatment groups, respectively. No difference were observed in the expression levels of AGC, OMC, CAT, SOD, and GSH-Px mRNAs in the heart of rats between the L-malate treatment and control groups. These results predicted that L-malate may increase the antioxidant capacity of mitochondria by enhancing the expression of mRNAs involved in the MAS and the antioxidant enzymes.

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