scholarly journals The chemokinetic response of human neutrophils

Blood ◽  
1986 ◽  
Vol 67 (4) ◽  
pp. 1036-1042 ◽  
Author(s):  
TH Howard
Keyword(s):  
Dose Dependence ◽  
Human Neutrophils ◽  
Maximal Response ◽  
Computer Assisted ◽  
Cellular Inflammatory Response ◽  
Blood Neutrophils ◽  
Response Increase ◽  
Flow Through ◽  
The Mean ◽  
Serial Addition

Abstract A computer-assisted single cell assay that allows quantification of the locomotive behavior of individual cells and a flow-through system that allows study of response of individual cells to stimulation were utilized to study the chemokinetic response of neutrophils. The range of basal mean rate of locomotion (mROL) and chemokinetic response to 10(-9) mol/L formylmethionyl leucyl phenylalanine (FMLP) was determined for neutrophils of eight normal adults. The basal mROL was 8.2 +/- 1.5 um/min and 6.2 +/- 1.0 um/min; the rate after 10(-9) mol/L fMLP was 12.1 +/- 2.1 and 9.5 +/- 1.8 um/min in 2.0 g% and 0.05 g% HSA, respectively. The mean increase in ROL for neutrophils was 50%. Assay with the flow-through system shows that the chemokinetic response-- increase in mROL of a population of neutrophils in response to 10(-9) mol/L--is due to an increase in ROL when cells are actively moving and not due to a decrease in the amount of time the cell spends inactive. Studies of individual cells within the populations show that chemokinetic response to 10(-9) mol/L fMLP is highly variable. The majority of cells (77%) respond with an increase in ROL; the minority (23%) are nonresponders that characteristically move at ROL greater than or equal to 14 um/min prior to stimulation and do not change ROL or exhibit a net decline in ROL in response to 10(-9) mol/L fMLP. The dose response of a population of neutrophils and of individual neutrophils to serial addition of 10(-10) to 10(-6) mol/L fMLP shows that the fMLP dose dependence for maximal chemokinetic response is highly variable among individual cells. Seventeen percent of cells do not respond to any fMLP concentration; 25% of neutrophils exhibit maximal response to 10(-10) mol/L fMLP, while 50% and 25% of cells showed peak chemokinetic response to 10(-9) mol/L and greater than or equal to 10(-8) mol/L fMLP, respectively. These studies document the variability in the locomotive responses of peripheral blood neutrophils. Understanding the causes of variability in the chemokinetic responsiveness of individual neutrophils may improve our understanding of how the cellular inflammatory response in man can be modulated.

scholarly journals The chemokinetic response of human neutrophils

Blood ◽  
1986 ◽  
Vol 67 (4) ◽  
pp. 1036-1042
Author(s):  
TH Howard
Keyword(s):  
Dose Dependence ◽  
Human Neutrophils ◽  
Maximal Response ◽  
Computer Assisted ◽  
Cellular Inflammatory Response ◽  
Blood Neutrophils ◽  
Response Increase ◽  
Flow Through ◽  
The Mean ◽  
Serial Addition

A computer-assisted single cell assay that allows quantification of the locomotive behavior of individual cells and a flow-through system that allows study of response of individual cells to stimulation were utilized to study the chemokinetic response of neutrophils. The range of basal mean rate of locomotion (mROL) and chemokinetic response to 10(-9) mol/L formylmethionyl leucyl phenylalanine (FMLP) was determined for neutrophils of eight normal adults. The basal mROL was 8.2 +/- 1.5 um/min and 6.2 +/- 1.0 um/min; the rate after 10(-9) mol/L fMLP was 12.1 +/- 2.1 and 9.5 +/- 1.8 um/min in 2.0 g% and 0.05 g% HSA, respectively. The mean increase in ROL for neutrophils was 50%. Assay with the flow-through system shows that the chemokinetic response-- increase in mROL of a population of neutrophils in response to 10(-9) mol/L--is due to an increase in ROL when cells are actively moving and not due to a decrease in the amount of time the cell spends inactive. Studies of individual cells within the populations show that chemokinetic response to 10(-9) mol/L fMLP is highly variable. The majority of cells (77%) respond with an increase in ROL; the minority (23%) are nonresponders that characteristically move at ROL greater than or equal to 14 um/min prior to stimulation and do not change ROL or exhibit a net decline in ROL in response to 10(-9) mol/L fMLP. The dose response of a population of neutrophils and of individual neutrophils to serial addition of 10(-10) to 10(-6) mol/L fMLP shows that the fMLP dose dependence for maximal chemokinetic response is highly variable among individual cells. Seventeen percent of cells do not respond to any fMLP concentration; 25% of neutrophils exhibit maximal response to 10(-10) mol/L fMLP, while 50% and 25% of cells showed peak chemokinetic response to 10(-9) mol/L and greater than or equal to 10(-8) mol/L fMLP, respectively. These studies document the variability in the locomotive responses of peripheral blood neutrophils. Understanding the causes of variability in the chemokinetic responsiveness of individual neutrophils may improve our understanding of how the cellular inflammatory response in man can be modulated.

Stochastic homogenization and macroscopic modelling of composites and flow through porous media

2002 ◽  
pp. 337-378 ◽  
Author(s):  
Jozef Telega ◽  
Wlodzimierz Bielski
Keyword(s):  
Porous Medium ◽  
Random Distribution ◽  
Flow Through Porous Media ◽  
Macroscopic Models ◽  
Linear Elastic ◽  
Multi Scale ◽  
Convergence Method ◽  
Flow Through ◽  
The Mean ◽  
Random Porous Medium

The aim of this contribution is mainly twofold. First, the stochastic two-scale convergence in the mean developed by Bourgeat et al. [13] is used to derive the macroscopic models of: (i) diffusion in random porous medium, (ii) nonstationary flow of Stokesian fluid through random linear elastic porous medium. Second, the multi-scale convergence method developed by Allaire and Briane [7] for the case of several microperiodic scales is extended to random distribution of heterogeneities characterized by separated scales (stochastic reiterated homogenization). .

Study of pumping effect in flow-through electrolysers

1983 ◽  
Vol 48 (8) ◽  
pp. 2232-2248 ◽  
Author(s):  
Ivo Roušar ◽  
Michal Provazník ◽  
Pavel Stuhl
Keyword(s):  
Natural Convection ◽  
Balance Equation ◽  
Volume Fraction ◽  
Industrial Applications ◽  
Pumping Effect ◽  
Flow Through ◽  
The Mean ◽  
The Difference

In electrolysers with recirculation, where a gas is evolved, the pumping of electrolyte from a lower to a higher level can be effected by natural convection due to the difference between the densities of the inlet electrolyte and the gaseous emulsion at the outlet. An accurate balance equation for calculation of the rate of flow of the pumped liquid is derived. An equation for the calculation of the mean volume fraction of bubbles in the space between the electrodes is proposed and verified experimentally on a pilot electrolyser. Two examples of industrial applications are presented.

scholarly journals Ex vivo evaluation of a multilayered sealant patch for watertight dural closure: cranial and spinal models

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
A. Kinaci ◽  
S. van Thoor ◽  
S. Redegeld ◽  
M. Tooren ◽  
T. P. C. van Doormaal
Keyword(s):  
Ex Vivo ◽  
Resistance Test ◽  
Burst Pressure ◽  
Cerebrospinal Fluid Leakage ◽  
Computer Assisted ◽  
Dural Defect ◽  
Test Results ◽  
Physiological Conditions ◽  
Dural Attachment ◽  
The Mean

AbstractCerebrospinal fluid leakage is a frequent complication after cranial and spinal surgery. To prevent this complication and seal the dura watertight, we developed Liqoseal, a dural sealant patch comprising a watertight polyesterurethane layer and an adhesive layer consisting of poly(DL-lactide-co-ε-caprolactone) copolymer and multiarmed N-hydroxylsuccinimide functionalized polyethylene glycol. We compared acute burst pressure and resistance to physiological conditions for 72 h of Liqoseal, Adherus, Duraseal, Tachosil, and Tisseel using computer-assisted models and fresh porcine dura. The mean acute burst pressure of Liqoseal in the cranial model (145 ± 39 mmHg) was higher than that of Adherus (87 ± 47 mmHg), Duraseal (51 ± 42 mmHg) and Tachosil (71 ± 16 mmHg). Under physiological conditions, cranial model resistance test results showed that 2 of 3 Liqoseal sealants maintained dural attachment during 72 hours as opposed to 3 of 3 for Adherus and Duraseal and 0 of 3 for Tachosil. The mean burst pressure of Liqoseal in the spinal model (233 ± 81 mmHg) was higher than that of Tachosil (123 ± 63 mmHg) and Tisseel (23 ± 16 mmHg). Under physiological conditions, spinal model resistance test results showed that 2 of 3 Liqoseal sealants maintained dural attachment for 72 hours as opposed to 3 of 3 for Adherus and 0 of 3 for Duraseal and Tachosil. This novel study showed that Liqoseal is capable of achieving a strong watertight seal over a dural defect in ex vivo models.

scholarly journals The Flow through the Gulf of Mexico

2019 ◽  
Vol 49 (6) ◽  
pp. 1381-1401 ◽  
Author(s):  
J. Candela ◽  
J. Ochoa ◽  
J. Sheinbaum ◽  
M. López ◽  
P. Pérez-Brunius ◽  
...  
Keyword(s):  
Gulf Of Mexico ◽  
Straits Of Florida ◽  
The North ◽  
Astronomical Tide ◽  
Flow Through ◽  
Sverdrup Transport ◽  
The Mean ◽  
Yucatan Channel ◽  
Range Variation

AbstractFour years (September 2012 to August 2016) of simultaneous current observations across the Yucatan Channel (~21.5°N) and the Straits of Florida (~81°W) have permitted us to investigate the characteristics of the flow through the Gulf of Mexico. The average transport in both channels is 27.6 Sv (1 Sv = 106 m3 s−1), in accordance with previous estimates. At the Straits of Florida section, the transport related to the astronomical tide explains 55% of the observed variance with a mixed semidiurnal/diurnal character, while in the Yucatan Channel tides contribute 82% of the total variance and present a dominant diurnal character. At periods longer than a week the transports in the Yucatan and Florida sections have a correlation of 0.83 without any appreciable lag. The yearly running means of the transport time series in both channels are well correlated (0.98) and present a 3-Sv range variation in the 4 years analyzed. This long-term variability is well related to the convergence of the Sverdrup transport in the North Atlantic between 14.25° and 18.75°N. Using 2 years (July 2014–July 2016) of simultaneous currents observations in the Florida section, the Florida Cable section (~26.7°N), and a section across the Old Bahama Channel (~78.4°W), a mean northward transport of 28.4, 31.1, and 1.6 Sv, respectively, is obtained, implying that only 1.1 Sv is contributed by the Northwest Providence Channel to the mean transport observed at the Cable section during this 2-yr period.

Studies on the storage of potatoes. III. The composition of the atmosphere in a potato clamp

1950 ◽  
Vol 40 (3) ◽  
pp. 227-232 ◽  
Author(s):  
E. M. Crook ◽  
D. J. Watson
Keyword(s):  
Multiple Regression ◽  
Wind Velocity ◽  
Practical Importance ◽  
Oxygen Depletion ◽  
Unsaturated Compounds ◽  
The Face ◽  
Flow Through ◽  
The Mean ◽  
Oxygen Concentrations

The CO2 concentration in the atmosphere of a potato clamp varied between 0·06 and 0·86%. The sum of CO2 and oxygen concentrations remained approximately constant at 21%. The CO2 concentration increased with time from December to April. This was attributed to increase in the rate of respiration of the potatoes caused by rise of temperature. Wind blowing in the direction normal to the face of the clamp reduced the COa concentration, presumably by causing external air to flow through the clamp coverings. A multiple regression of CO2 concentration on temperature of the potatoes at the time of sampling, and on the mean component of wind velocity normal to the clamp face estimated over a period of 3 hr. before the time of sampling, accounted for 64% of the variance between sampling occasions.Unsaturated compounds were detected in the clamp atmosphere by absorption in bromine; the concentration of these, expressed as ethylene, varied between 0·004 and 0·025%.The magnitude of CO2 accumulation and oxygen depletion in the clamp atmosphere was too small to produce effects of practical importance on the storage behaviour of the potatoes. If the unsaturated compounds were ethylene, the concentration present was sufficient to cause appreciable retardation of sprouting.

Numerical Study on the Effect of Bend Angle on Turbulent Flow through Oscillating Bend

2009 ◽  
Vol 4 (1) ◽  
Author(s):  
Elham Ameri ◽  
M Nasr Esfahany
Keyword(s):  
Numerical Study ◽  
Bend Angle ◽  
Curvature Radius ◽  
Primary Flow ◽  
Mean Velocity ◽  
Contour Map ◽  
Curved Pipes ◽  
Flow Through ◽  
The Mean ◽  
Turbulent Air Flow

The effect of the bend angle on the unsteady developing turbulent air flow through oscillating circular-sectioned curved pipes with the various angles of 180°, 135° and 90° was investigated numerically. The bends had a diameter of 106 mm and a curvature radius ratio of 6.0 with long, straight upstream and downstream sections. Results of the mean velocity and static pressure were obtained at a Reynolds number of 31200 and at various longitudinal stations. The velocity of the primary flow was illustrated in the form of contour map and vector diagram. From the inlet plane of the three oscillating bends to the angle of 45°, the velocity fields in 180°, 90° and 135° bends are similar. The high velocity regions, however, occur near the upper and lower parts in 90° and 180° bends, respectively.

scholarly journals Patients with Achilles Tendon Rupture Have a Degenerated Contralateral Achilles Tendon: An Elastography Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Qianru Li ◽  
Qi Zhang ◽  
Yehua Cai ◽  
Yinghui Hua
Keyword(s):  
Achilles Tendon ◽  
Axial Strain ◽  
Achilles Tendon Rupture ◽  
Cross Sectional Area ◽  
Control Group ◽  
Computer Assisted ◽  
Study Group ◽  
Sectional Area ◽  
Cross Sectional ◽  
The Mean

Purpose. To evaluate differences of Achilles tendon (AT) hardness and morphology between asymptomatic tendons in patients with acute AT ruptures on the contralateral side and asymptomatic tendons in healthy people by using computer-assisted quantification on axial-strain sonoelastography (ASE). Methods. The study consisted of 33 asymptomatic tendons in 33 patients (study group) and 34 tendons in 19 healthy volunteers (control group). All the tendons were examined by both ASE and conventional ultrasound. Computer-assisted quantification on ASE was applied to extract hardness variables, including the mean (Hmean), 20th percentile (H20), median (H50) and skewness (Hsk) of the hardness within tendon, and the ratio of the mean hardness within tendon to that outside tendon (Hratio) and three morphological variables: the thickness (THK), cross-sectional area, and eccentricity (ECC) of tendons. Results. The Hmean, Hsk, H20, H50, and Hratio in the proximal third of the tendon body in study group were significantly smaller than those in control group (Hmean: 0.43±0.09 vs 0.50±0.07, p=0.001; Hsk: -0.53±0.51 vs -1.09±0.51, p<0.001; H20: 0.31±0.10 vs 0.40±0.10, p=0.001; H50: 0.45±0.10 vs 0.53±0.08, p<0.001; Hratio: 1.01±0.25 vs 1.20±0.23, p=0.003). The THK and cross-sectional area of tendons in the study group were larger than those in the control group (p<0.05). Conclusions. As a quantitative objective method, the computer-assisted ASE reveals that the asymptomatic ATs contralateral to acute rupture are softer than those of healthy control group at the proximal third and the asymptomatic tendons in people with rupture history are thicker, larger, and rounder than those of normal volunteers especially at the middle and distal thirds of AT body.

scholarly journals Electrospun Polydioxanone Loaded With Chloroquine Modulates Template-Induced NET Release and Inflammatory Responses From Human Neutrophils

2021 ◽  
Vol 9 ◽  
Author(s):  
Allison E. Fetz ◽  
Shannon E. Wallace ◽  
Gary L. Bowlin
Keyword(s):  
Growth Factor ◽  
Inflammatory Responses ◽  
Human Peripheral Blood ◽  
Early Time ◽  
Human Neutrophils ◽  
Late Time ◽  
Dependent Manner ◽  
Blood Neutrophils ◽  
Net Release

The implantation of a biomaterial quickly initiates a tissue repair program initially characterized by a neutrophil influx. During the acute inflammatory response, neutrophils release neutrophil extracellular traps (NETs) and secrete soluble signals to modulate the tissue environment. In this work, we evaluated chloroquine diphosphate, an antimalarial with immunomodulatory and antithrombotic effects, as an electrospun biomaterial additive to regulate neutrophil-mediated inflammation. Electrospinning of polydioxanone was optimized for rapid chloroquine elution within 1 h, and acute neutrophil-biomaterial interactions were evaluated in vitro with fresh human peripheral blood neutrophils at 3 and 6 h before quantifying the release of NETs and secretion of inflammatory and regenerative factors. Our results indicate that chloroquine suppresses NET release in a biomaterial surface area–dependent manner at the early time point, whereas it modulates signal secretion at both early and late time points. More specifically, chloroquine elution down-regulates interleukin 8 (IL-8) and matrix metalloproteinase nine secretion while up-regulating hepatocyte growth factor, vascular endothelial growth factor A, and IL-22 secretion, suggesting a potential shift toward a resolving neutrophil phenotype. Our novel repurposing of chloroquine as a biomaterial additive may therefore have synergistic, immunomodulatory effects that are advantageous for biomaterial-guided in situ tissue regeneration applications.

scholarly journals Toxoplasma gondii Extends the Life Span of Infected Human Neutrophils by Inducing Cytosolic PCNA and Blocking Activation of Apoptotic Caspases

mBio ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Tatiane S. Lima ◽  
Sharmila Mallya ◽  
Allen Jankeel ◽  
Ilhem Messaoudi ◽  
Melissa B. Lodoen
Keyword(s):  
Cell Death ◽  
Toxoplasma Gondii ◽  
Life Span ◽  
Peripheral Blood ◽  
Productive Infection ◽  
Human Neutrophils ◽  
Serum Starvation ◽  
Rna Seq ◽  
Content Type ◽  
Blood Neutrophils

ABSTRACT Toxoplasma gondii is an intracellular protozoan parasite that has the remarkable ability to infect and replicate in neutrophils, immune cells with an arsenal of antimicrobial effector mechanisms. We report that T. gondii infection extends the life span of primary human peripheral blood neutrophils by delaying spontaneous apoptosis, serum starvation-induced apoptosis, and tumor necrosis alpha (TNF-α)-mediated apoptosis. T. gondii blockade of apoptosis was associated with an inhibition of processing and activation of the apoptotic caspases caspase-8 and -3, decreased phosphatidylserine exposure on the plasma membrane, and reduced cell death. We performed a global transcriptome analysis of T. gondii-infected peripheral blood neutrophils using RNA sequencing (RNA-Seq) and identified gene expression changes associated with DNA replication and DNA repair pathways, which in mature neutrophils are indicative of changes in regulators of cell survival. Consistent with the RNA-Seq data, T. gondii infection upregulated transcript and protein expression of PCNA, which is found in the cytosol of human neutrophils, where it functions as a key inhibitor of apoptotic pro-caspases. Infection of neutrophils resulted in increased interaction of PCNA with pro-caspase-3. Inhibition of this interaction with an AlkB homologue 2 PCNA-interacting motif (APIM) peptide reversed the infection-induced delay in cell death. Taken together, these findings indicate a novel strategy by which T. gondii manipulates cell life span in primary human neutrophils, which may allow the parasite to maintain an intracellular replicative niche and avoid immune clearance. IMPORTANCE Toxoplasma gondii is an obligate intracellular parasite that can cause life-threatening disease in immunocompromised individuals and in the developing fetus. Interestingly, T. gondii has evolved strategies to successfully manipulate the host immune system to establish a productive infection and evade host defense mechanisms. Although it is well documented that neutrophils are mobilized during acute T. gondii infection and infiltrate the site of infection, these cells can also be actively infected by T. gondii and serve as a replicative niche for the parasite. However, there is a limited understanding of the molecular processes occurring within T. gondii-infected neutrophils. This study reveals that T. gondii extends the life span of human neutrophils by inducing the expression of PCNA, which prevents activation of apoptotic caspases, thus delaying apoptosis. This strategy may allow the parasite to preserve its replicative intracellular niche.

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