scholarly journals OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hongjia Zhang ◽  
Tao Wu ◽  
Zhao Li ◽  
Kai Huang ◽  
Na-Eun Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Haplotype Analysis ◽  
Seedling Stage ◽  
Growth And Yield ◽  
Wild Type ◽  
Gata Transcription Factor ◽  
Localization Signal ◽  
New Germplasm

Abstract Background Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear. Results In this study, a GATA-type zinc finger transcription factor OsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains. OsGATA16 was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression of OsGATA16 improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genes OsWRKY45–1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter of OsWRKY45–1 and repressed its expression. In addition, haplotype analysis showed that OsGATA16 polarized between the two major rice subspecies japonica and indica, and had a non-synonymous SNP8 (336G) associated with cold tolerance. Conclusion OsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45–1, OsSRFP1, OsCYL4 and OsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336G) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding.

scholarly journals OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice

2021 ◽  
Author(s):  
Hongjia Zhang ◽  
Tao Wu ◽  
Zhao Li ◽  
Kai Huang ◽  
Na-Eun Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Low Temperature ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Seedling Stage ◽  
Transcriptional Analysis ◽  
Luciferase Reporter ◽  
Positive Role ◽  
Breeding Programs ◽  
Gata Transcription Factor

Abstract BackgroundCold stress in rice is a major abiotic stress that adversely affects growth and substantially reduces rice yield. Identification of cold-related functional rice genes is important for breeding programs aimed at increasing resilience and yield in rice crops. GATA-family transcription factors involve diverse function in rice, however, their roles in the response to low-temperature stress remain unclear.ResultsA GATA-type zinc finger transcription factor, OsGATA16, that increases cold tolerance in rice. OsGATA16 is an OsGATA subfamily-II protein and contains eleven putative phosphorylation sites, NLS, and several conserved domains. Overexpression of OsGATA16 increased tolerance to cold stress at seedling stage. Transcriptional analysis showed that OsGATA16 was induced by cold and ABA treatments, but was repressed by drought, cytokinin, and JA. OsGATA16 was expressed in all plant tissues, with highest expression in panicles. Subcellular localization and transcriptional analysis indicated that OsGATA16 acted as a nuclear-targeted transcriptional suppressor. Four cold-related genes (OsWRKY45-1, OsSRFP1, OsCYL4, and OsMYB30) were repressed in OsGATA16-overexpression lines compared with wild type after low-temperature exposure. Yeast one-hybrid and Dual-luciferase reporter assays showed that OsGATA16 bound to the promoter of OsWRKY45-1 and repressed its expression. Eleven SNPs within OsGATA16 were identified and haplotype analysis showed a polarization between Japonica and Indicia subspecies. A non-synonymous SNP was identified that explained differences in cold tolerance among the 137 rice accessions.ConclusionA novel GATA transcription factor, OsGATA16, plays a positive role in cold tolerance at the seedling stage in rice by direct repression of OsWRKY45-1 expression. One SNP was identified that explained cold tolerance differences among rice accessions. These results support future breeding programs to improve cold tolerance in commercial rice crops.

scholarly journals Combining QTL-seq and linkage mapping to fine map a candidate gene in qCTS6 for cold tolerance at the seedling stage in rice

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Luomiao Yang ◽  
Jingguo Wang ◽  
Zhenghong Han ◽  
Lei Lei ◽  
Hua Long Liu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Regulatory Gene ◽  
Snp Markers ◽  
Seedling Stage ◽  
Pcr Analysis ◽  
Sequencing Data ◽  
Coding Region ◽  
Rice Varieties ◽  
Major Qtl

Abstract Background Cold stress caused by low temperatures is an important factor restricting rice production. Identification of cold-tolerance genes that can stably express in cold environments is crucial for molecular rice breeding. Results In this study, we employed high-throughput quantitative trait locus sequencing (QTL-seq) analyses in a 460-individual F2:3 mapping population to identify major QTL genomic regions governing cold tolerance at the seedling stage in rice. A novel major QTL (qCTS6) controlling the survival rate (SR) under low-temperature conditions of 9°C/10 days was mapped on the 2.60-Mb interval on chromosome 6. Twenty-seven single-nucleotide polymorphism (SNP) markers were designed for the qCST6 region based on re-sequencing data, and local QTL mapping was conducted using traditional linkage analysis. Eventually, we mapped qCTS6 to a 96.6-kb region containing 13 annotated genes, of which seven predicted genes contained 13 non-synonymous SNP loci. Quantitative reverse transcription PCR analysis revealed that only Os06g0719500, an OsbZIP54 transcription factor, was strongly induced by cold stress. Haplotype analysis confirmed that +376 bp (T>A) in the OsbZIP54 coding region played a key role in regulating cold tolerance in rice. Conclusion We identified OsbZIP54 as a novel regulatory gene associated with rice cold-responsive traits, with its Dongfu-104 allele showing specific cold-induction expression serving as an important molecular variation for rice improvement. This result is expected to further exploration of the genetic mechanism of rice cold tolerance at the seedling stage and improve cold tolerance in rice varieties by marker-assisted selection.

scholarly journals Molecular and Phenotypic Characterization for Cold Tolerance in Rice (Oryza sativa L.)

2020 ◽  
Vol 23 (2) ◽  
pp. 1-15
Author(s):  
PS Biswas ◽  
H Khatun ◽  
M Anisuzzaman
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Reproductive Stage ◽  
Seedling Stage ◽  
Phenotypic Characterization ◽  
Breeding Lines ◽  
Upgma Dendrogram ◽  
Ssr Loci

Cold stress affects growth and development of rice plants from germination to reproductive stages. Thirty breeding lines/varieties including tolerant and susceptible check varieties were evaluated for cold tolerance at seedling and reproductive stage using artificially induced low temperature situations to understand the genetic relationship among them and to identify promising donors for cold tolerance. Three breeding lines, BR8253-9-3-4-3, IR77497-31-2-3-1and IR2266-42-6-2 demonstrated higher cold tolerance for the seedling stage (LD score <3.0). Molecular analysis revealed 68% genetic similarity among the genotypes. On average 64% of the breeding lines shared common alleles at any given locus ranging from 27% (RM22870) to 97% (RM186). UPGMA dendrogram showed that majority of the breeding lines showing 4-5 degrees of LD were grouped together. Two breeding lines, BR8258-7-1-5-2B2 and BR8258-7-1-5-2B3 having 86% common alleles showed low leaf discoloration (LD: 4) at seedling stage coupled with relatively low reduction in spikelet fertility (45.8% and 48.1%) and grain yield (43.3% and 39.1%). The gene diversity and PIC value revealed that seven SSR loci (RM1282, RM600, RM5349, RM6972, RM5847, RM5911, and RM511) might be the best markers to differentiate between tolerant and susceptible genotypes at reproductive stage. The breeding lines with tolerance to cold stress at the seedling and the reproductive stages identified from this study, and the SSR markers mentioned above could be useful for developing cold tolerance rice. Bangladesh Rice j. 2019, 23(2): 1-15

scholarly journals Overexpression of a New Zinc Finger Protein Transcription FactorOsCTZFP8Improves Cold Tolerance in Rice

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Yong-Mei Jin ◽  
Rihua Piao ◽  
Yong-Feng Yan ◽  
Mojun Chen ◽  
Ling Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Zinc Finger ◽  
Transgenic Rice ◽  
Abiotic Stresses ◽  
Zinc Finger Protein ◽  
Single Copy ◽  
Finger Protein ◽  
Protein Transcription

Cold stress is one of the most important abiotic stresses in rice. C2H2zinc finger proteins play important roles in response to abiotic stresses in plants. In the present study, we isolated and functionally characterized a new C2H2zinc finger protein transcription factorOsCTZFP8in rice.OsCTZFP8encodes a C2H2zinc finger protein, which contains a typical zinc finger motif, as well as a potential nuclear localization signal (NLS) and a leucine-rich region (L-box). Expression ofOsCTZFP8was differentially induced by several abiotic stresses and was strongly induced by cold stress. Subcellular localization assay and yeast one-hybrid analysis revealed that OsCTZFP8 was a nuclear protein and has transactivation activity. To characterize the function ofOsCTZFP8in rice, the full-length cDNA ofOsCTZFP8was isolated and transgenic rice with overexpression ofOsCTZFP8driven by the maize ubiquitin promoter was generated usingAgrobacterium-mediated transformation. Among 46 independent transgenic lines, 6 single-copy homozygous overexpressing lines were selected by Southern blot analysis and Basta resistance segregation assay in both T1and T2generations. Transgenic rice overexpressingOsCTZFP8exhibited cold tolerant phenotypes with significantly higher pollen fertilities and seed setting rates than nontransgenic control plants. In addition, yield per plant ofOsCTZFP8-expressing lines was significantly (p<0.01) higher than that of nontransgenic control plants under cold treatments. These results demonstrate thatOsCTZFP8was a C2H2zinc finger transcription factor that plays an important role in cold tolerance in rice.

Citrus sinensis CBF1 Functions in Cold Tolerance by Modulating Putrescine Biosynthesis Through Regulation of ARGININE DECARBOXYLASE

2021 ◽  
Author(s):  
Jie Song ◽  
Hao Wu ◽  
Feng He ◽  
Jing Qu ◽  
Yue Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Stress Response ◽  
Transgenic Plants ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Arginine Decarboxylase ◽  
Wild Type ◽  
Shed Light

Abstract C-repeat (CRT) binding factors (CBFs) are well known to act as crucial transcription factors that function in cold stress response. Arginine decarboxylase (ADC)-mediated putrescine biosynthesis has been reported to be activated in plants exposed to cold conditions, but it remains elusive whether CBFs can regulate ADC expression and putrescine accumulation. In this study, we show that cold up-regulated ADC gene (CsADC) and elevation of endogenous putrescine content in sweet orange (Citrus sinensis). Promoter of CsADC contains two CRT sequences that are canonical elements recognized by CBFs. Sweet orange genome contains four CBFs (CsCBF1-4), in which CsCBF1 was significantly induced by cold. CsCBF1, located in the nucleus, was demonstrated to bind directly and specifically to the promoter of CsADC and acted as a transcriptional activator. Overexpression of CsCBF1 led to notable elevation of CsADC and putrescine level in sweet orange transgenic plants, along with remarkably enhanced cold tolerance, relative to the wild type (WT). However, pretreatment with D-arginine, an ADC inhibitor, caused prominent reduction of endogenous putrescine level in the overexpressing lines, accompanied by greatly compromised cold tolerance. Taken together, these results demonstrate that CBF1 of sweet orange directly regulates ADC expression and modulates putrescine synthesis for orchestrating the cold tolerance. Our findings shed light into the transcriptional regulation of putrescine accumulation through targeting the ADC gene in the presence of cold stress. Meanwhile, this study illustrates a new mechanism underlying the CBF-mediated cold stress response.

Identification of rice mutants tolerant to cold stress at the germination stage by TILLING.

2021 ◽  
pp. 111-119
Author(s):  
Viviane Kopp da Luz ◽  
Vívian Ebeling Viana ◽  
Gabriela Magalhães da Fonseca ◽  
Camila Pegoraro ◽  
Luciano Carlos da Maia ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Common Factor ◽  
Chemical Mutagenesis ◽  
Mutation Induction ◽  
Wild Type ◽  
Mutant Genotype ◽  
Rice Mutant ◽  
Germination Ability ◽  
Germination Stage

Abstract Cold stress is a common factor affecting rice culture in temperate regions, which impairs seed germination, crop establishment and grain yield. This work aimed to identify, through a TILLING assay, rice mutant families displaying cold tolerance during the germination stage. The mutant analyses were performed in 4000 M3 plants obtained through chemical mutagenesis with ethyl methanesulfonate. We screened for mutations in the Os03g0103300 (qLTG3-1) gene, which is responsible for cold tolerance during germination. The TILLING assay identified a mutant (516 A3) which was tested for germination efficiency in cold stress (13°C). The mutant genotype showed a higher relative performance in germination and germination velocity index, which was more than 50% higher compared with wild-type. The mutation induction was efficient in creating genetic variability for cold stress tolerance during germination. Gene expression analyses demonstrate that Os03g0103300 was downregulated in stage S3 in the mutant and wild-type plants germinated under cold stress. However, downregulation in the Os03g0103300 gene was less severe in the mutant, which suggests that the expression related to germination ability under cold stress may be detected in the previous stages, embryo activation and weakening of the tissues that cover the embryo. Overall, the mutant 516 A3 presents a new genetic variant for cold tolerance during germination.

scholarly journals ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

2022 ◽  
Vol 23 (2) ◽  
pp. 606
Author(s):  
Yongbo Hong ◽  
Hui Wang ◽  
Yizhou Gao ◽  
Yan Bi ◽  
Xiaohui Xiong ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Abiotic Stress ◽  
Carboxylic Acid ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Cold Tolerance ◽  
Transcriptional Activation ◽  
Altered Expression ◽  
Erf Transcription Factor

We previously showed that overexpression of the rice ERF transcription factor gene OsBIERF3 in tobacco increased resistance against different pathogens. Here, we report the function of OsBIERF3 in rice immunity and abiotic stress tolerance. Expression of OsBIERF3 was induced by Xanthomonas oryzae pv. oryzae, hormones (e.g., salicylic acid, methyl jasmonate, 1-aminocyclopropane-1-carboxylic acid, and abscisic acid), and abiotic stress (e.g., drought, salt and cold stress). OsBIERF3 has transcriptional activation activity that depends on its C-terminal region. The OsBIERF3-overexpressing (OsBIERF3-OE) plants exhibited increased resistance while OsBIERF3-suppressed (OsBIERF3-Ri) plants displayed decreased resistance to Magnaporthe oryzae and X. oryzae pv. oryzae. A set of genes including those for PRs and MAPK kinases were up-regulated in OsBIERF3-OE plants. Cell wall biosynthetic enzyme genes were up-regulated in OsBIERF3-OE plants but down-regulated in OsBIERF3-Ri plants; accordingly, cell walls became thicker in OsBIERF3-OE plants but thinner in OsBIERF3-Ri plants than WT plants. The OsBIERF3-OE plants attenuated while OsBIERF3-Ri plants enhanced cold tolerance, accompanied by altered expression of cold-responsive genes and proline accumulation. Exogenous abscisic acid and 1-aminocyclopropane-1-carboxylic acid, a precursor of ethylene biosynthesis, restored the attenuated cold tolerance in OsBIERF3-OE plants while exogenous AgNO3, an inhibitor of ethylene action, significantly suppressed the enhanced cold tolerance in OsBIERF3-Ri plants. These data demonstrate that OsBIERF3 positively contributes to immunity against M. oryzae and X. oryzae pv. oryzae but negatively regulates cold stress tolerance in rice.

scholarly journals Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii

2006 ◽  
Vol 5 (8) ◽  
pp. 1388-1398 ◽  
Author(s):  
Raúl García-Salcedo ◽  
Antonio Casamayor ◽  
Amparo Ruiz ◽  
Asier González ◽  
Catarina Prista ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Profiling ◽  
Single Gene ◽  
Ion Homeostasis ◽  
Carbon Sources ◽  
Debaryomyces Hansenii ◽  
Wild Type ◽  
Gata Factor ◽  
Gata Transcription Factor ◽  
Atpase Gene

ABSTRACT The yeast Debaryomyces hansenii has a remarkable capacity to proliferate in salty and alkaline environments such as seawater. A screen for D. hansenii genes able to confer increased tolerance to high pH when overexpressed in Saccharomyces cerevisiae yielded a single gene, named here DhGZF3, encoding a putative negative GATA transcription factor related to S. cerevisiae Dal80 and Gzf3. Overexpression of this gene in wild-type S. cerevisiae increased caffeine and rapamycin tolerance, blocked growth in low glucose concentrations and nonfermentable carbon sources, and resulted in lithium- and sodium-sensitive cells. Sensitivity to salt could be attributed to a reduced cation efflux, most likely because of a decrease in expression of the ENA1 Na+-ATPase gene. Overexpression of DhGZF3 did not affect cell growth in a gat1 mutant but was lethal in the absence of Gln3. These are positive factors that oppose both Gzf3 and Dal80. Genome-wide transcriptional profiling of wild-type cells overexpressing DhGZF3 shows decreased expression of a number of genes that are usually induced in poor nitrogen sources. In addition, the entire pathway leading to Lys biosynthesis was repressed, probably as a result of a decrease in the expression of the specific Lys14 transcription factor. In conclusion, our results demonstrate that DhGzf3 can play a role as a negative GATA transcription factor when expressed in S. cerevisiae and that it most probably represents the only member of this family in D. hansenii. These findings also point to the GATA transcription factors as relevant elements for alkaline-pH tolerance.

scholarly journals OsCRP1, a Ribonucleoprotein Gene, Regulates Chloroplast mRNA Stability That Confers Drought and Cold Tolerance

2021 ◽  
Vol 22 (4) ◽  
pp. 1673
Author(s):  
Seung Woon Bang ◽  
Ho Suk Lee ◽  
Su-Hyun Park ◽  
Dong-Keun Lee ◽  
Jun Sung Seo ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Cold Tolerance ◽  
Molecular Mechanisms ◽  
Rna Binding ◽  
Mrna Levels ◽  
Wild Type ◽  
Rna Immunoprecipitation ◽  
Chloroplast Rna ◽  
Chloroplast Mrna

Chloroplast ribonucleoproteins (cpRNPs) are nuclear-encoded and highly abundant proteins that are proposed to function in chloroplast RNA metabolism. However, the molecular mechanisms underlying the regulation of chloroplast RNAs involved in stress tolerance are poorly understood. Here, we demonstrate that CHLOROPLAST RNA-BINDING PROTEIN 1 (OsCRP1), a rice (Oryza sativa) cpRNP gene, is essential for stabilization of RNAs from the NAD(P)H dehydrogenase (NDH) complex, which in turn enhances drought and cold stress tolerance. An RNA-immunoprecipitation assay revealed that OsCRP1 is associated with a set of chloroplast RNAs. Transcript profiling indicated that the mRNA levels of genes from the NDH complex significantly increased in the OsCRP1 overexpressing compared to non-transgenic plants, whereas the pattern in OsCRP1 RNAi plants were opposite. Importantly, the OsCRP1 overexpressing plants showed a higher cyclic electron transport (CET) activity, which is essential for elevated levels of ATP for photosynthesis. Additionally, overexpression of OsCRP1 resulted in significantly enhanced drought and cold stress tolerance with higher ATP levels compared to wild type. Thus, our findings suggest that overexpression of OsCRP1 stabilizes a set of mRNAs from genes of the NDH complex involved in increasing CET activity and production of ATP, which consequently confers enhanced drought and cold tolerance.

Chemotaxis and Transcription Factor Activation of Wild-Type and CCR6-Transfected RLE-6TN

Pneumologie ◽  
2012 ◽  
Vol 66 (11) ◽  
Author(s):  
K Hoehne ◽  
H Eibel ◽  
M Grimm ◽  
M Idzko ◽  
J Müller-Quernheim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Wild Type ◽  
Transcription Factor Activation
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