scholarly journals Aspergillus flavus originated pure compound as a potential antibacterial

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Saeed Ullah Khattak ◽  
Ghosia Lutfullah ◽  
Zafar Iqbal ◽  
Jamshaid Ahmad ◽  
Irshad Ur Rehman ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Growth ◽  
In Silico Analysis ◽  
Binding Pocket ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Exact Mass ◽  
Antibacterial Potential

Abstract Problem Background Penicillin was the first and most famous fungal secondary metabolite used as broad spectrum antibiotic that revolutionarised pharmaceutical research and also saved millions of lives. The over optimistic belief in 1967 that sufficient antibiotics had been discovered to defeat infectious diseases was quickly crashed with the appearance of multidrug resistant (MDR) bacteria in 1990s. This has posed a serious threat to mankind. Although scientists are making efforts to synthesize and discover new antibiotics there are not enough new drugs in pharmaceutical pipeline to beat the pace at which MDR bacteria are emerging. In view of this there is an urgent and serious medical need for new bioactive compounds to be discovered to treat infections caused by MDR pathogens. The present study is aimed to investigate the antibacterial potential of Aspergillus flavus originated compounds that may act as drug leads to treat future infections. Methodology Among the 6 isolated fungal strains from the rhizosphere of Mentha piperetta, one was processed for isolation of secondary metabolites on the basis of preliminary antibacterial testing. Observation of morphological and microscopic features helped in identification of the fungal strain as Aspergillus flavus. Potato Dextrose Agar (PDA) medium was used for fungal growth while Czapec Yeast Broth (CYB) medium was used for production of fungal metabolites. Column chromatography technique was utilized for purification of compound from crude fungal extract and the mass of the compound was determined using Liquid Chromatography Mass Spectrometry (LCMS) method. Structure elucidation of the pure compound was performed using 500 Varian Nuclear Magnetic Resonance (NMR) machine. Docking was performed using Glide SP algorithm. Agar well diffusion method was used to determine the invitro antibacterial potential of the compound against two MDR bacterial strains i.e. Staphylococcus aureus and Proteus vulgaris. For this a total of 4 dose concentrations i.e. (100, 250, 500, 1000 μg mL− 1) of the compound were prepared and applied to bacterial strains on Mueller Hinton agar using tetracycline as control. Results The chemical name of the purified compound from A. flavus was determined as (2E)-3-[(3S, 4R)-8-hydroxy-3, 4-dimethyl-1-oxo-3, 4-dihydro-1H-2- benzopyran-7-yl] prop-2-enoic acid with the formula C14H14O5 and exact mass of 262.08. The in-Silico analysis showed that this compound has the potential to inhibit the binding pocket of S. aureus TyrRS (1JII) with docking score of − 8.67 Kcal mole− 1. The results obtained from invitro experiments were encouraging as at 1000 μg mL− 1 the compound showed 58.8% inhibition against S. aureus and 28% inhibition against P. vulgaris. Conclusions The pure compound with formula C14H14O5 and exact mass of 262 exhibited antibacterial potential both insilico and invitro against both Gram negative and Gram positive bacteria. The compound was more active against S. aureus in comparison to P. vulgaris. From the obtained results it is concluded that this compound can be used as potent antibacterial candidate but further studies will be needed prior to its use as antibiotic.

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

scholarly journals IN VITRO ANTIOXIDANT AND ANTIBACTERIAL POTENTIAL OF MEDICINAL PLANT CERASTIUM FONTANUM

2021 ◽  
Vol 51 (3) ◽  
pp. 145-151
Author(s):  
Himayat Ullah ◽  
Muhammad Mustafa ◽  
Muhammad Israr ◽  
Zahid Ali ◽  
M. Nouman ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Liquid Extraction ◽  
New Drugs ◽  
Diffusion Method ◽  
Aqueous Fraction ◽  
Gram Negative ◽  
Liquid Liquid Extraction ◽  
Antibacterial Potential ◽  
Agar Well Diffusion Method

Naturally God gifted medicinal plants which contain more potentially active compounds their characterization and isolation are very important and can provide us a great help in making new drugs to cure many diseases. Our current attempt was made to obtain the ethanolic extracts of medicinal plant Cerastium fontanum using a hot continuous soxhlet process and also via maceration method. The concentrated dried fraction extracts of Cerastium fontanum such as aqueous, n-hexane, ethyl acetate and dichloromethane were achieved using liquid-liquid extraction which were then evaluated for in vitro antioxidants potential using DPPH (1,1-diphenyl-2-picryl-hydrazyl) assay and also agar-well diffusion method were used for antimicrobial potential. In vitro antioxidants inhibition potential were measured through spectrophotometer at various concentration (500 to 62.5µg/mL) prepared in standard solvent. Among all extracts aqueous fraction showed IC50 = 2.9 ± 0.05mg/mL maximum potency towards stable DPPH much closer to standard control acarbose IC50 = 2.61 ± 0.01mg/mL. The antibacterial activity results indicated that all fractions found active against both strains (gram positive and gram negative) of bacteria. In various extracts only the aqueous fraction extracts showed excellent inhibition potential against Staphylococcus aureus and Escherichia Coli. While dichloromethane fraction extracts were found active only with gram negative strain. Thus the Cerastium fontanum extracts possess much higher inhibition potential then standard available antibiotics in the market.

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

scholarly journals Iriflophenone-3-C-β-d Glucopyranoside from Dryopteris ramosa (Hope) C. Chr. with Promising Future as Natural Antibiotic for Gastrointestinal Tract Infections

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1128
Author(s):  
Muhammad Ishaque ◽  
Yamin Bibi ◽  
Samha Al Ayoubi ◽  
Saadia Masood ◽  
Sobia Nisa ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Bioactive Compound ◽  
2D Nmr ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Aqueous Fraction ◽  
Tract Infections ◽  
Antibacterial Potential

Ethnopharmacological approaches provide clues for the search of bioactive compounds. Dryopteris ramosa (Hope) C. Chr. (plant family: Dryopteridaceae) is an ethnomedicinal plant of the Galliyat region of Pakistan. The aqueous fraction (AqF) of D. ramosa is being used by inhabitants of the Galliyat region of Pakistan to treat their gastrointestinal tract ailments, especially those caused by bacteria. The aims of the present study were as follows: (i) to justify the ethnomedicinal uses of the AqF of D. ramosa; (ii) to isolate a bioactive compound from the AqF of D. ramosa; and (iii) to evaluate the antibacterial and cytotoxic potential of the isolated compound. Column chromatography (CC) techniques were used for the isolation studies. Spectroscopic techniques (UV–Vis, MS, 1&2D NMR) were used for structural elucidation. The agar-well diffusion method was used to evaluate the antibacterial potential of “i3CβDGP” against five bacterial strains, and compare it with the known antibiotic “Cefixime”. The brine shrimp lethality test (BSLT) was used for cytotoxic studies. The AqF of D. ramosa afforded “iriflophenone-3-C-β-D glucopyranoside (i3CβDGP)” when subjected to LH20 Sephadex, followed by MPLC silica gel60, and purified by preparative TLC. The “i3CβDGP” showed a strong potential (MIC = 31.1 ± 7.2, 62.5 ± 7.2, and 62.5 ± 7.2 µg/mL) against Klebsiella pneumoniae, Staphylococcus aureus, and Escherichia coli, respectively. On the other hand, the least antibacterial potential was shown by “i3CβDGP” (MIC = 125 ± 7.2 µg/mL), against Bacillus subtilis, in comparison to Cefixime (MIC = 62.5 ± 7.2µg/mL). The cytotoxicity of “i3CβDGP” was significantly low (LD50 = 10.037 ± 2.8 µg/mL) against Artemia salina nauplii. This study not only justified the ethnomedicinal use of D. ramosa, but also highlighted the importance of ethnomedicinal knowledge. Further studies on AqF and other fractions of D. ramosa are in progress.

Evaluation of antibacterial potential of various plant extracts against clinically important skin infectious bacterial strains

2020 ◽  
Vol 18 ◽  
Author(s):  
Syeda Kahkashan Kazmi ◽  
Naheed Kauser ◽  
Ayisha Aman ◽  
Aqsa Idrees ◽  
Rashida Rahmat Zohra ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Plant Extracts ◽  
Skin Diseases ◽  
Curcuma Longa ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Santalum Album ◽  
Bacterial Strains ◽  
Citrus Peel ◽  
Antibacterial Potential

Background: At the present time skin problems need to address seriously, antibiotic resistance development is very fast in skin infectious bacteria. For the prevention of all types of skin infections, natural plant extracts can provide suitable defense line because they contain active compounds. Wrinkles, sunburn, itching, black heads, white heads and uneven tone of the skin are the collective issues related to skin. Extensive use of antibiotics for skin diseases is restricted due to the development of worse antibiotic resistance. Objective: In this study the antibacterial potential of plant extracts was assessed against skin infectious bacteria to get alternative cure of skin diseases. Method: Staphylococcus aureus, Staphylococcus epidermidis strains were isolated from clinical samples and E.coli was used as non-pathogenic control strain from Jinnah University for Women strain repository. Their antibacterial activity was performed against eight plant extracts through well diffusion method. Result: Among eight plant extracts, Neem (Azadirachta indica), Turmeric (Curcuma longa), Sandalwood (Santalum album), Shahtara (Fumaria parviflora) Chirayata (Swertia chirayita) and commercially available mixed Citrus peel showed good inhibition of bacterial growth. Discussion: Natural sources such as fruits, herbs, minerals, leaves, flowers, roots, honey and rose water are now becoming part of skin care and treatment products because they possess antimicrobial activities. Skin products with herbal ingredients are now evolving as suitable solutions to the existing skin problems.

scholarly journals Antibacterial potential and ethnomedical relevance of Kumaun Himalayan Gymnosperms

2016 ◽  
Vol 5 (5) ◽  
pp. 190-200
Author(s):  
Savita Joshi ◽  
◽  
S.C. Sati ◽  
Parikshit Kumar ◽  
◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
New Drugs ◽  
Diffusion Method ◽  
Taxus Baccata ◽  
Cedrus Deodara ◽  
Kumaun Himalaya ◽  
Antibacterial Potential

Ethnomedicinal information is one of the powerful criteria for new drugs discoveries therefore; present investigation was carried out to evaluate the ethnomedicinal and antibacterial potential of traditional Kumaun Himalayan gymnosperms. Besides, an antibacterial value index and relative antibacterial activity of studied plants have been established. Forty four plant leaves extracts in different organic solvents (methanol, ethanol, chloroform and hexane) of 11 gymnosperms (Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana, Pinus wallichiana and Taxus baccata) occurring in Kumaun Himalaya were screened for their antimicrobial activity against five pathogenic bacteria using disc diffusion method. The antibacterial activity of studied gymnospermic plant extracts against a panel of bacteria was found effective at 1000 µg/ml. The MIC and MBC values of each extract (where ZOI ≥ 15 mm) were also determined. The methanol extract of screened gymnosperms were found the most effective against all the bacteria (54% to 81%), followed by ethanol extract (45-72%), hexane extract (18-27%), while in chloroform extract it ranged 9-27% only. The extracts of G. biloba exhibited superior Relative Antibacterial Activity (RAA, 20%), followed by A. cunninghamii and P. wallichiana (12% RAA, each). The lowest RAA value was observed for C. torulosa (1%). All data were also analyzed for determination of an Antibacterial Value Index (ABVI) for each studied species of gymnosperm. G. biloba had maximum ABVI i.e. 90 % followed by A. cunninghamii and P. wallichiana (ABVI, 55% each). C. torulosa showed the least ABVI and RAA i.e. 5% and 11%, respectively. The present work fully highlighted the utility of traditionally known 11 gymnosperms of Kumaun Himalaya for their antibacterial activities against pathogenic bacteria.

Evaluation of Antibacterial Potential of New Acid Dyes Based on Substituted Aryl Amines and Amino Hydroxy Sulfonic Acid

2020 ◽  
Vol 42 (5) ◽  
pp. 783-783
Author(s):  
Zeeshan Akhtar Zeeshan Akhtar ◽  
Syed Imran Ali Syed Imran Ali ◽  
Naseem Abbas Naseem Abbas ◽  
Muhammad Ali Muhammad Ali ◽  
Muhammad Yasir Khan Muhammad Yasir Khan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sulfonic Acid ◽  
Diffusion Method ◽  
Dye Molecules ◽  
Bacterial Strains ◽  
Acid Dyes ◽  
Aryl Amines ◽  
Coupling Components ◽  
Antibacterial Potential

Six newly synthesized acid dye derivatives were evaluated for their in vitro antimicrobial activity against four (04) Gram positive (Staphylococcus aureus, Staphylococcus lugdunensis, Streptococcus pneumoniae and Bacillus subtilis) and six (06) Gram negative (Pseudomonas aeruginosa, Acinetobacter baumannii, Citrobacter freundii, Klebsiella pneumoniae,Pseudomonas fluorescens and Shigella sonnei) bacterial strains. The dyes were synthesized using substituted aryl amines and substituted amino hydroxy sulfonic acid as diazo and coupling components, respectively. The antimicrobial activity was explored by Agar-Well Diffusion method using 100 mg/ml dye concentration. The results demonstrated that the dyes possess varying degrees of activities against the bacterial strains tested and the structure of the dye molecules was found to have considerable influence on their antimicrobial activity.

scholarly journals ANTIMICROBIAL PROPERTIES OF LEPTADENIA HASTATA (PERS) DECNE LEAVES EXTRACT

2018 ◽  
Vol 10 (2) ◽  
pp. 149 ◽  
Author(s):  
Isaac John Umaru ◽  
Fasihuddin A. Badruddin ◽  
Zaini B. Assim ◽  
Hauwa A. Umaru
Keyword(s):  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
New Drugs ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Medical Practitioners ◽  
Antibacterial Potential ◽  
Agar Well Diffusion Method

Objective: This study was carried out with an objective to investigate the antibacterial potential of five leaf extract of Leptadenia hastata plant on some bacteria.Methods: Leptadenia hastata extracts were evaluated for potential antimicrobial properties. The leaves of the plant were extracted with n-hexane, dichloromethane, ethyl acetate, chloroform and methanol and then vaporized to give respective extracts. Antimicrobial activity against Escherichia coli, salmonella typhi, staphylococcus aureus and Klebsielia pneumonia, was determined by an agar well diffusion method. The optical density of the broth using UV mini spectrophotometer and zone of inhibition by the crude extract were determined.Results: The results showed that of n-hexane, ethyl acetate, dichloromethane and methanol extracts of Leptadenia hastata, methanolic and chloroform extracts displayed more activity with 1.10±0.10 mmab and 0.97±0.06 mmab where aSignificantly (p<0.05) higher compared to different extract at the same concentration bSignificantly (p<0.05) lower compared to the control, than others at 25-1000 ppm per well of the extracts tested.Conclusion: The present results showed the potential of the medicinal plant used by traditional herbal medical practitioners as natural antimicrobial agents, thus can be further used to determine the bioactive products that may provide as leads in the development of new drugs.

scholarly journals Antifungal and antitoxin effects of propolis and its nanoemulsion formulation against Aspergillus flavus isolated from human sputum and milk powder samples

2021 ◽  
pp. 2306-2312
Author(s):  
Alshimaa A. Hassanien ◽  
Eman M. Shaker ◽  
Eman E. El-Sharkawy ◽  
Walaa M. Elsherif
Keyword(s):  
Aspergillus Flavus ◽  
High Performance ◽  
Animal Feed ◽  
Fungal Spores ◽  
Milk Powder ◽  
Fungal Growth ◽  
Diffusion Method ◽  
Quantitative Detection ◽  
Safety Level ◽  
Powder Samples

Background and Aim: Aspergillus flavus causes human and animal diseases through either inhalation of fungal spores or ingestion of mycotoxins as aflatoxins produced in human and animal feed as secondary metabolites. This study was aimed to detect the incidence of A. flavus and its aflatoxins in human sputum and milk powder samples and explore the efficacy of pure propolis (PP) and propolis nanoemulsion (PNE) as natural decontaminants against fungal growth and its released aflatoxins. Materials and Methods: A. flavus was isolated by mycological culture and identified macroscopically and microscopically. Coconut agar medium and thin-layer chromatography (TLC) were used to qualitatively detect aflatoxins in the isolated strains. Toxins were extracted from toxigenic strains by the fast extraction technique. The quantitative detection of toxin types was explored by high-performance liquid chromatography (HPLC). PNE was prepared by a novel method using natural components and characterized by Fourier-transform infrared spectroscopy, Zetasizer, and transmission electron microscopy. The effects of PP and PNE on A. flavus growth and its toxin were determined by the well-diffusion method and HPLC. Results: The mycological culture showed that 30.9% and 29.2% of sputum and milk powder samples were positive for A. flavus, respectively. TLC confirmed the production of 61.8% and 63.2% aflatoxin by the isolated strains in sputum and milk powder, respectively. PP and PNE showed antifungal activity on A. flavus growth with mean±standard error (SE) inhibition zones of 27.55±3.98 and 39.133±5.32 mm, respectively. HPLC revealed positive contamination of toxin extracts with AFB1, AFB2, and AFG2 at 0.57±0.026, 0.28±0.043, and 0.1±0.05 mg/L, respectively. After treatment with PP and PNE, a significant decrease in AFB1, AFB2, and AFG2 concentrations was observed. Conclusion: This study suggested using propolis and its nanoformulation as antifungal and antitoxins in human medicine and the food industry to increase the food safety level and stop food spoilage.

scholarly journals Microbial Study on NiO – CuO Nano Particles by Grinding Technique

2021 ◽  
Author(s):  
S Ramakrishnan ◽  
R. Vaishnavi ◽  
S. Ramakrishnan ◽  
M. Revathi ◽  
S. Rajakarthihan
Keyword(s):  
Metal Oxide Nanoparticles ◽  
Fungal Growth ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
Nano Particles ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Visible Absorption ◽  
Grinding Technique ◽  
Bacteria And Fungi

Abstract One of the biggest concerns around the world seems to be the fight against bacteria and fungi. This major challenge can be overcome with the addition of a combination of metal and metal oxides. Metal oxide nanoparticles have emerged as effective antimicrobial and anti-fungal agents. In this work, A Grinding technique has been used to synthesize NiO – CuO nanoparticles. Nanoparticles were characterized using X-ray powder diffraction (XRD) analysis which confirmed the formation of all the phases, Fourier Transform-Infrared Spectroscopy (FTIR), UV–Visible Absorption Spectroscopy and Scanning Electron Microscope (SEM) analysis were showed the vibrational structure, optical and uniformity of particle size of the nanoparticles. Bacterial and fungal growth study was confirmed by the disc diffusion method. The prepared nanoparticles showed detrimental effects against Streptococcus, Staphylococcus aureus (bacterial strains), and Aspergillus Niger (fungal strains). A concentration-dependent growth inhibition effect of nanoparticles on both bacteria and fungi was observed. The prepared composites were better active in both bacteria and fungies.

Sign in / Sign up

Export Citation Format

Share Document