scholarly journals Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jing Chen ◽  
Hui Zeng ◽  
Xiumei Zhang
Keyword(s):  
Leaf Nitrogen ◽  
Ananas Comosus ◽  
Leaf Color ◽  
Phosphate Transporters ◽  
Tropical Fruit ◽  
Inorganic P ◽  
Ammonium Transporters ◽  
N Assimilation ◽  
Bhlh Transcription Factors ◽  
Insight Into

Abstract Background Pineapple (Ananas comosus L. Merr.) is the third most important tropical fruit in China. In other crops, farmers can easily judge the nutritional requirements from leaf color. However, concerning pineapple, it is difficult due to the variation in leaf color of the cultivated pineapple varieties. A detailed understanding of the mechanisms of nutrient transport, accumulation, and assimilation was targeted in this study. We explored the D-leaf nitrogen (N), phosphorus (P), and potassium (K) contents, transcriptome, and metabolome of seven pineapple varieties. Results Significantly higher N, P, and K% contents were observed in Bali, Caine, and Golden pineapple. The transcriptome sequencing of 21 libraries resulted in the identification of 14,310 differentially expressed genes in the D-leaves of seven pineapple varieties. Genes associated with N transport and assimilation in D-leaves of pineapple was possibly regulated by nitrate and ammonium transporters, and glutamate dehydrogenases play roles in N assimilation in arginine biosynthesis pathways. Photosynthesis and photosynthesis-antenna proteins pathways were also significantly regulated between the studied genotypes. Phosphate transporters and mitochondrial phosphate transporters were differentially regulated regarding inorganic P transport. WRKY, MYB, and bHLH transcription factors were possibly regulating the phosphate transporters. The observed varying contents of K% in the D-leaves was associated to the regulation of K+ transporters and channels under the influence of Ca2+ signaling. The UPLC-MS/MS analysis detected 873 metabolites which were mainly classified as flavonoids, lipids, and phenolic acids. Conclusions These findings provide a detailed insight into the N, P, K% contents in pineapple D-leaf and their transcriptomic and metabolomic signatures.

Nitrogen Assimilation Enzyme Activities in Witchweed (Striga) in Hosts Presence and Absence

Weed Science ◽  
1996 ◽  
Vol 44 (2) ◽  
pp. 224-232 ◽  
Author(s):  
Imuetinyan Igbinnosa ◽  
Patrick A. Thalouarn
Keyword(s):  
Host Plant ◽  
Enzyme Activities ◽  
Host Plants ◽  
Nitrogen Assimilation ◽  
Reductase Activity ◽  
Growth Conditions ◽  
Plant Growth And Development ◽  
Continuous Darkness ◽  
N Assimilation ◽  
Insight Into

N fertilizers suppress witchweed plant growth and development, thus reducing the severity of parasite attack and increasing host yield simultaneously. However, the underlying physiological mode of N action occurring within the parasite cells remains largely unknown. This study aims at screening for the effects of N forms and different growth conditions on some N assimilation enzymes in witchweed seedlings grown aseptically without host plant, and in pots with host plants. Results show that supply of N in NH4+or urea forms resulted in 83 to 92% reduction in nitrate reductase activity (NRc), compared with control. Increasing NO3−concentrations from 0 mM to 100 mM, led to a corresponding increase in NRc in giant witchweed. NRc of giant witchweed seedlings grown under light and dark cycles were about 270 times higher than seedlings grown in continuous darkness. A combination of NH4+and NO3−, resulted in increased giant witchweed NRc, compared with NH4+or NO3−supplied singly. Highest shoot development and NRc was at NH4+and NO3−ratio 1:1, followed by ratios 1:3, 3:1, 0:1, and 1:0, respectively. Addition of N in soils resulted in increased NRc, followed by rapid deterioration and death of giant witchweed plants. NRc, GSc, and GDHc in witchweed, maize, cowpea, and tobacco were affected by diurnal fluctuations with higher enzyme activities occurring during the day than at night. Higher GSc than GDHc suggests that NH4+assimilation occurs mainly through the GS pathway in witchweed plants. NRc and GDHc were two and four times higher in giant witchweed grown in aseptic media without host plant, than that grown in potted soils with host plants. These findings provide insight into the physiological mode of N action and their implications on witchweed control.

scholarly journals Response of ‘Desirable’ and ‘Kiowa’ Pecan to a Late-spring Freeze

2008 ◽  
Vol 18 (3) ◽  
pp. 455-459 ◽  
Author(s):  
M. Lenny Wells
Keyword(s):  
Nitrogen Concentration ◽  
Nutrient Status ◽  
Leaf Nitrogen ◽  
Late Spring ◽  
Leaf Chlorophyll ◽  
Mouse Ear ◽  
Freeze Damage ◽  
Chlorophyll Index ◽  
Insight Into ◽  
Leaf Symptoms

This study was established to assess the effects of a severe late spring freeze on flowering, shoot growth, leaf nutrient status, and the retention of fruit developing from secondary buds of pecan [Carya illinoinensis (Wangenh.) K.Koch]. Freeze damage appears to have a significant influence on pecan physiology and fruit retention. ‘Desirable’ produced a crop of pistillate flowers from secondary buds after the freeze; however, many of these flowers were abnormal in appearance. Freeze-damaged ‘Desirable’ trees exhibited shorter shoots, reduced flower and fruit retention, a lower chlorophyll index, and decreased leaf nitrogen concentration compared with nondamaged trees. Leaf zinc concentration was higher in freeze-injured ‘Desirable’ trees than in nondamaged trees. Freeze-damaged ‘Kiowa’ trees had longer shoots and failed to produce a crop of pistillate flowers from secondary buds on most shoots. Freeze damage led to the appearance of mouse-ear leaf symptoms and reduced leaf chlorophyll index, leaf nitrogen, and leaf magnesium concentrations in ‘Kiowa’. Leaf phosphorous and leaf potassium concentrations were higher in freeze-injured ‘Kiowa’ trees than in nondamaged trees. These observations provide insight into the potential response of bearing orchard trees injured by a late spring freeze.

scholarly journals Nitrogen assimilation in the bromeliad Ananas comosus var. ananassoides (Baker) Coppens & F.Leal grown in vitro with different sources of inorganic nitrogen

Hoehnea ◽  
2020 ◽  
Vol 47 ◽  
Author(s):  
Priscila Primo Andrade Silva ◽  
Ivomar Aparecido Medina ◽  
Jorge Luiz Marx Young ◽  
Vívian Tamaki
Keyword(s):  
Nitrogen Assimilation ◽  
Inorganic Nitrogen ◽  
Ananas Comosus ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Ex Vitro ◽  
N Assimilation ◽  
Modified Ms Medium ◽  
Different Sources

ABSTRACT Ananas comosus var. ananassoides (Baker) Coppens & F.Leal is a native ornamental bromeliad of the endangered biome Cerrado. Therefore, approaches aimed at the preservation of this species, such as in vitro cultivation and micropropagation are needed. Nitrogen (N) is absorbed by plants, mainly as NO3- and/or NH4+, and assimilated into amino acids. The aim of this work was to evaluate the N assimilation in this bromeliad. Plants were grown in vitro for seven months in modified MS medium with 15, 30, 60, and 90 mM of N as NO3-, NH4+ or NH4NO3, and then transferred to ex vitro conditions for acclimatization. Plants grown with NH4+ had high mortality. During acclimatization plants cultivated with 30, 60, and 90 mM of N as NH4NO3 showed higher biomass. With regard to N assimilation, GS and NR showed the highest activity in plants cultivated with NH4NO3, whereas plants cultivated with NH4+ had the highest GDH activity. Consequently, in vitro and ex vitro cultivation of this species with 60 mM N as NH4NO3 is recommended.

scholarly journals Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Ananas comosus)

Plants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 275 ◽  
Author(s):  
Cao ◽  
Cheng ◽  
Zhang ◽  
Aslam ◽  
Yan ◽  
...  
Keyword(s):  
Plant Cell Wall ◽  
Developmental Stages ◽  
Functional Characterization ◽  
Gene Families ◽  
Cellulose Synthase ◽  
Ananas Comosus ◽  
Tropical Fruit ◽  
Genome Wide ◽  
Gene Structures

The cellulose synthase (Ces) and cellulose synthase-like (Csl) gene families belonging to the cellulose synthase gene superfamily, are responsible for the biosynthesis of cellulose and hemicellulose of the plant cell wall, and play critical roles in plant development, growth and evolution. However, the Ces/Csl gene family remains to be characterized in pineapple, a highly valued and delicious tropical fruit. Here, we carried out genome-wide study and identified a total of seven Ces genes and 25 Csl genes in pineapple. Genomic features and phylogeny analysis of Ces/Csl genes were carried out, including phylogenetic tree, chromosomal locations, gene structures, and conserved motifs identification. In addition, we identified 32 pineapple AcoCes/Csl genes with 31 Arabidopsis AtCes/Csl genes as orthologs by the syntenic and phylogenetic approaches. Furthermore, a RNA-seq investigation exhibited the expression profile of several AcoCes/Csl genes in various tissues and multiple developmental stages. Collectively, we provided comprehensive information of the evolution and function of pineapple Ces/Csl gene superfamily, which would be useful for screening out and characterization of the putative genes responsible for tissue development in pineapple. The present study laid the foundation for future functional characterization of Ces/Csl genes in pineapple.

Ginkgo biloba microRNA profiling reveals new insight into leaf color mutation

2020 ◽  
Vol 265 ◽  
pp. 109189 ◽  
Author(s):  
Yaqiong Wu ◽  
Xia Li ◽  
Tongli Wang ◽  
Jing Guo ◽  
Fuliang Cao ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Leaf Color ◽  
Microrna Profiling ◽  
Color Mutation ◽  
Insight Into

scholarly journals Genome-wide identification and expression analysis of the ERF transcription factor family in pineapple (Ananas comosus (L.) Merr.)

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10014 ◽  
Author(s):  
Youmei Huang ◽  
Yanhui Liu ◽  
Man Zhang ◽  
Mengnan Chai ◽  
Qing He ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Developmental Process ◽  
Economic Value ◽  
Ananas Comosus ◽  
Functional Verification ◽  
Tropical Fruit ◽  
Genome Wide ◽  
Erf Family ◽  
Gene Structures ◽  
And Function

Pineapple (Ananas comosus (L.) Merr.) is an important tropical fruit with high economic value. The quality and yield of pineapple will be affected by various environmental conditions. Under adverse conditions, plants can produce a complex reaction mechanism to enhance their resistance. It has been reported that the member of ethylene responsive transcription factors (ERFs) plays a crucial role in plant developmental process and stress response. However, the function of these proteins in pineapple remains limited. In this study, a total of 74 ERF genes (AcoERFs) were identified in pineapple genome, named from AcoERF1 to AcoERF74, and divided into 13 groups based on phylogenetic analysis. We also analyzed gene structure, conserved motif and chromosomal location of AcoERFs, and the AcoERFs within the same group possess similar gene structures and motif compositions. Three genes (AcoERF71, AcoERF73 and AcoERF74) were present on unanchored scaffolds, so they could not be conclusively mapped on chromosome. Synteny and cis-elements analysis of ERF genes provided deep insight into the evolution and function of pineapple ERF genes. Furthermore, we analyzed the expression profiling of AcoERF in different tissues and developmental stages, and 22 AcoERF genes were expressed in all examined tissues, in which five genes (AcoERF13, AcoERF16, AcoERF31, AcoERF42, and AcoERF65) had high expression levels. Additionally, nine AcoERF genes were selected for functional verification by qRT-PCR. These results provide useful information for further investigating the evolution and functions of ERF family in pineapple.

scholarly journals Identifikasi Molekuler dan Analisis Kekerabatan Aksesi Nenas Menggunakan Marka RAPD Menunjang Perakitan Varietas Unggul Baru

2019 ◽  
Vol 28 (1) ◽  
pp. 1
Author(s):  
Sri Hadiati ◽  
Riry Prihatini ◽  
Ellina Mansyah
Keyword(s):  
Molecular Identification ◽  
Genetic Similarity ◽  
Rapd Marker ◽  
Computer Software ◽  
Similarity Coefficient ◽  
Ananas Comosus ◽  
Similarity Coefficients ◽  
Tropical Fruit ◽  
New Varieties ◽  
Polymorphism Level

<p><strong>(</strong><em><strong>Molecular Identification and Relationships Among Several Pineapple Accessions Using RAPD Marker to Support the Assembling New Varieties</strong></em><strong>)</strong></p><p>Produksi dan produktivitas nenas dapat ditingkatkan antara lain melalui penggunaan varietas unggul. Dalam perakitan varietas, dibutuhkan informasi hubungan kekerabatan antartetuanya agar diperoleh efek heterosis yang tinggi melalui kegiatan identifikasi secara molekuler. Penelitian bertujuan (1) mengetahui tingkat polimorfisme primer yang digunakan,(2) mengidentifikasi fragmen DNA spesifik yang membedakan individu atau kelompok individu nenas, dan (3) mengetahui hubungan kekerabatan antarspesies dan aksesi nenas. Penelitian dilaksanakan mulai bulan Mei–Desember 2014 di Laboratorium Uji Mutu Benih dan Molekuler Balai Penelitian Tanaman Buah Tropika. Sampel yang digunakan sebanyak 19 aksesi dari empat spesies nenas (Ananas comosus, A. bracteatus, A. lucidus, dan A. nanus). Sebanyak 20 marka rapid amplified polymorphism DNA (RAPD) digunakan dalam analisis. Data diskor secara biner kemudian dianalisis menggunakan program NTSYSpc 2,1x. Hasil analisis menunjukkan bahwa polimorfisme 20 primer yang diuji berkisar 33–100% dengan rata-rata 87%. Primer dengan tingkat polimorfisme 100%, yaitu RAPD3, OPA13, OPAV3, OPC12, OPC16, dan OPY15. Kelompok Cayenne dicirikan oleh marka RAPD1 ukuran1.000 base-pair (bp) dan OPAV3 700 bp. Kelompok Queen dapat diidentifikasikan oleh marka RAPD3 ukuran 700 bp, kelompok Spanish dengan marka RAPD2 dan RAPD3 ukuran 1.500 bp. Analisis kluster menunjukkan bahwa 19 aksesi yang diuji terpisah menjadi enam kelompok pada koefisien kesamaan genetik 0,75, yaitu kelompok Queen, Cayenne, Spanish, A. bracteatus, A. lucidus, dan A. nanus. Aksesi yang diuji mempunyai keragaman genetik yang luas dengan koefisien kesamaan genetik berkisar 0,41–0,85. Aksesi yang mempunyai kesamaan genetik tertinggi, yaitu antara N-73 dengan BB (0,85) dan terkecil, yaitu antara N-94 (A. nanus) dengan N-18 (Green Spanish) sebesar 0,41. Implikasi hasil penelitian adalah aksesi yang mempunyai kesamaan genetik tinggi salah satunya dapat dieliminasi untuk efisiensi dalam pengelolaan plasma nutfah, sedangkan aksesi-aksesi yang memiliki kesamaan genetik kecil, baik digunakan sebagai tetua persilangan agar diperoleh variabilitas genetik yang luas dan efek heterosis yang tinggi.</p><p><strong>Keywords</strong></p><p><em>Ananas</em> spp.; Identifikasi; Karakterisasi; Kekerabatan genetik; Molekuler.</p><p><strong>Abstract</strong></p><p>Pineapple production and productivity can increased by the use of superior variety. Pertaining to variety assembling, the relationship information among parents are needed to gain heterosis effect through molecular identification activity. This research was aimed to (1) determine the level of polymorphism primers used, (2) identify specific DNA fragments which discrete individual or group of pineapple, and (3) reveal genetic relationship among pineapple species and accessions. The experiment was conducted on May to December 2014 in Seeds Quality Testing and Molecular Laboratory of Indonesian Tropical Fruit Research Institute. Nineteen accessions from four species (Ananas comosus, A. brachteatus, A. lucidus, and A. nanus) of pineapple were used as samples. Twenty rapid amplified polymorphism DNA (RAPD) markers were used on molecular analysis. The data were scored binary and then they were analyzed using NTSYSpc 2.1x computer software. The analysis showed that the 20 primers had 33–100%  polymorphic with 87% in average. Primers with 100% polymorphism level were RAPD3, OPA13, OPAV3, OPC12, OPC16, and OPY15. Cayenne group could be denoted with RAPD1 and OPAV3 markers by 1,000 base-pairs (bp) and 700 bp band, respectively. Meanwhile the Queen group can be identified by 700 bp band  RAPD3 marker. The Spanish group can be specified by1,500 bp band RAPD2 and RAPD3 markers. Based on cluster analysis the 19  accessions were separated  into six groups with 0.75 genetic similarity coefficient i.e., Queen, Cayenne, Spanish, A. bracteatus, A. lucidus, and A. nanus. These accessions had a wide genetic diversity with 0.41 to (0.85) genetic similarity coefficients. The highest genetic similarity coefficient (0.85) was determined between N-73 and BB, whereas the lowest value down to 0.41 was indicated on N-94 (A. nanus) and N-18 (Green Spanish). The implications of this research are that one of two accessions that have high genetic similarities can be eliminated for efficiency in the management of germplasm. While accessions which  have little genetic similarity are both used as crosses parent in order to obtain wide genetic variability and high heterosis effects.</p>

Improved High-Quality Genome Assembly and Annotation of Pineapple (Ananas comosus) Cultivar MD2 Revealed Extensive Haplotype Diversity and Diversified FRS/FRF Gene Family

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 52
Author(s):  
Ashley G. Yow ◽  
Hamed Bostan ◽  
Raúl Castanera ◽  
Valentino Ruggieri ◽  
Molla F. Mengist ◽  
...  
Keyword(s):  
Gene Prediction ◽  
Haplotype Diversity ◽  
Fold Increase ◽  
Ananas Comosus ◽  
High Quality ◽  
Tropical Fruit ◽  
Related Sequence ◽  
New Genes ◽  
High Level ◽  
High Quality Genome

Pineapple (Ananas comosus (L.) Merr.) is the second most important tropical fruit crop globally, and ‘MD2’ is the most important cultivated variety. A high-quality genome is important for molecular-based breeding, but available pineapple genomes still have some quality limitations. Here, PacBio and Hi-C data were used to develop a new high-quality MD2 assembly and gene prediction. Compared to the previous MD2 assembly, major improvements included a 26.6-fold increase in contig N50 length, phased chromosomes, and >6000 new genes. The new MD2 assembly also included 161.6 Mb additional sequences and >3000 extra genes compared to the F153 genome. Over 48% of the predicted genes harbored potential deleterious mutations, indicating that the high level of heterozygosity in this species contributes to maintaining functional alleles. The genome was used to characterize the FAR1-RELATED SEQUENCE (FRS) genes that were expanded in pineapple and rice. Transposed and dispersed duplications contributed to expanding the numbers of these genes in the pineapple lineage. Several AcFRS genes were differentially expressed among tissue-types and stages of flower development, suggesting that their expansion contributed to evolving specialized functions in reproductive tissues. The new MD2 assembly will serve as a new reference for genetic and genomic studies in pineapple.

Sign in / Sign up

Export Citation Format

Share Document