scholarly journals Ectopic expression of TaBG1 increases seed size and alters nutritional characteristics of the grain in wheat but does not lead to increased yields

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Matthew J. Milner ◽  
Sarah Bowden ◽  
Melanie Craze ◽  
Emma J. Wallington
Keyword(s):  
Protein Content ◽  
Seed Size ◽  
Ectopic Expression ◽  
N Fertilization ◽  
Essential Elements ◽  
Nutritional Profile ◽  
Control Seed ◽  
Wheat Gene ◽  
Lower Protein ◽  
The Cost

Abstract Background Grain size is thought to be a major component of yield in many plant species. Here we set out to understand if knowledge from other cereals such as rice could translate to increased yield gains in wheat and lead to increased nitrogen use efficiency. Previous findings that the overexpression of OsBG1 in rice increased yields while increasing seed size suggest translating gains from rice to other cereals may help to increase yields. Results The orthologous genes of OsBG1 were identified in wheat. One homoeologous wheat gene was cloned and overexpressed in wheat to understand its role in controlling seed size. Potential alteration in the nutritional profile of the grains were also analyzed in wheat overexpressing TaBG1. It was found that increased TaBG1-A expression could indeed lead to larger seed size but was linked to a reduction in seed number per plant leading to no significant overall increase in yield. Other important components of yield such as biomass or tillering did not change significantly with increased TaBG1-A expression. The nutritional profile of the grain was altered, with a significant decrease in the Zn levels in the grain associated with increased seed size, but Fe and Mn concentrations were unchanged. Protein content of the wheat grain also fell under moderate N fertilization levels but not under deficient or adequate levels of N. Conclusions TaBG1 does control seed size in wheat but increasing the seed size per se does not increase yield and may come at the cost of lower concentrations of essential elements as well as potentially lower protein content. Nevertheless, TaBG1 could be a useful target for further breeding efforts in combination with other genes for increased biomass.

scholarly journals TaBG1 Increases Seed Size and Alters Nutritional Characteristics of the Grain in Wheat But Does Lead to Increased Yields.

2021 ◽  
Author(s):  
Matthew J. Milner ◽  
Sarah Bowden ◽  
Melanie Craze ◽  
Emma J. Wallington
Keyword(s):  
Protein Content ◽  
Seed Size ◽  
N Fertilization ◽  
Essential Elements ◽  
Nutritional Profile ◽  
Control Seed ◽  
Nutritional Characteristics ◽  
Wheat Gene ◽  
Lower Protein ◽  
The Cost

Abstract Background: Grain size is thought to be a major component of yield in many plant species. Here we set out to understand if knowledge from other cereals such as rice could translate to increased yield gains in wheat and lead to increased nitrogen use efficiency. Previous findings that the overexpression of OsBG1 in rice increased yields while increasing seed size suggest translating gains from rice to other cereals may help to increase yields. Results: The orthologous genes of OsBG1 were identified in wheat. One homoeologous wheat gene was cloned and overexpressed in wheat to understand its role in controlling seed size. Potential alteration in the nutritional profile of the grains were also analyzed in wheat overexpressing TaBG1. It was found that increased TaBG1-A expression could indeed lead to larger seed size but was linked to a reduction in seed number per plant leading to no significant overall increase in yield. Other important components of yield such as biomass or tillering did not change significantly with increased TaBG1-A expression. The nutritional profile of the grain was altered, with a significant decrease in the Zn levels in the grain associated with increased seed size, but Fe and Mn concentrations were unchanged. Protein content of the wheat grain also fell under moderate N fertilization levels but not under deficient or adequate levels of N.Conclusions: TaBG1 does control seed size in wheat but increasing the seed size per se does not increase yield and may come at the cost of lower concentrations of essential elements as well as potentially lower protein content. Nevertheless, TaBG1 could be a useful target for further breeding efforts in combination with other genes for increased biomass.

Evaluation of the chemical, antinutritional and antioxidant properties of composite flour comprising native and modified acha (digitaria exilis stapf) flour supplemented with mango kernel seed and soy cake flours

2021 ◽  
pp. 108201322199125
Author(s):  
Margaret A Olorunfemi ◽  
Olugbenga O Awolu ◽  
Victor N Enujiugha
Keyword(s):  
Protein Content ◽  
Dietary Fiber ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Composite Flour ◽  
Mango Kernel ◽  
Composite Flours ◽  
Lower Protein ◽  
Mineral Compositions ◽  
Dpph Radical Scavenging

Gluten-free flours that are nutritionally balanced with appropriate functional characteristics were developed by supplementation of native and modified acha flours with protein, dietary fiber and antioxidants-rich mango kernel and soy cakes flours. Acha flour was subjected to chemical and enzymatic modifications. The proximate, mineral compositions, bioactive and antinutrients properties of the composite flours were evaluated. The water content of the composite flours with native and chemically modified acha flour was between 7.62 and 9.30%, while that of enzymatic acha flour was between 10.12 and 10.79%. However, samples made with 20 and 30% incorporated mango kernel flour had around 13 and 19% increase in the protein content respectively, others including sample with enzymatically modified acha flour had lower protein content. On the other hand, all samples with enzymatically modified acha flour had between 83 and 100% increase in fibre content. The Na/K ratio of all the samples were less than one, as nutritionally required. Samples with enzymatically modified acha flour had best total flavonoid (0.03–0.77 mgGAE/g), total phenol (2.35–11.99 mgTAE/g) and DPPH radical scavenging activities (58.29–94.02%) contents. In addition, samples with enzymatically modified acha flour had the least antinutritional values. Although all the samples had values that were significantly (p ≥ 0.05) different, the samples had significant protein, dietary fiber, minerals and antioxidants contents, while the antinutritional contents were well lower than the standard.

scholarly journals Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhijuan Chen ◽  
Vanessa Lancon-Verdier ◽  
Christine Le Signor ◽  
Yi-Min She ◽  
Yun Kang ◽  
...  
Keyword(s):  
Association Study ◽  
Protein Content ◽  
Seed Size ◽  
Seed Protein ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Strong Positive Correlation ◽  
Seed Composition ◽  
Seed Traits ◽  
Genome Wide

AbstractGrain legumes are highly valuable plant species, as they produce seeds with high protein content. Increasing seed protein production and improving seed nutritional quality represent an agronomical challenge in order to promote plant protein consumption of a growing population. In this study, we used the genetic diversity, naturally present in Medicago truncatula, a model plant for legumes, to identify genes/loci regulating seed traits. Indeed, using sequencing data of 162 accessions from the Medicago HAPMAP collection, we performed genome-wide association study for 32 seed traits related to seed size and seed composition such as seed protein content/concentration, sulfur content/concentration. Using different GWAS and postGWAS methods, we identified 79 quantitative trait nucleotides (QTNs) as regulating seed size, 41 QTNs for seed composition related to nitrogen (i.e. storage protein) and sulfur (i.e. sulfur-containing amino acid) concentrations/contents. Furthermore, a strong positive correlation between seed size and protein content was revealed within the selected Medicago HAPMAP collection. In addition, several QTNs showed highly significant associations in different seed phenotypes for further functional validation studies, including one near an RNA-Binding Domain protein, which represents a valuable candidate as central regulator determining both seed size and composition. Finally, our findings in M. truncatula represent valuable resources to be exploitable in many legume crop species such as pea, common bean, and soybean due to its high synteny, which enable rapid transfer of these results into breeding programs and eventually help the improvement of legume grain production.

Effect of harvesting stage on cereal and legume forage production in low rainfall regions

1976 ◽  
Vol 86 (1) ◽  
pp. 155-161 ◽  
Author(s):  
A. Hadjichristodoulou
Keyword(s):  
Chemical Composition ◽  
Protein Content ◽  
Dry Matter ◽  
Moisture Stress ◽  
N Fertilization ◽  
Forage Production ◽  
Common Vetch ◽  
Three Stages ◽  
Harvesting Stage ◽  
Percentage Protein

SUMMARYThe effect of stage of harvesting on dry-matter (D.M.) yield and chemical composition of barley, wheat and the legumes common vetch (F. sativa), woollypod vetch (F. dasycarpa) and fodder peas (P. sativum) were studied in Cyprus under low rainfall conditions in a series of trials sown in four successive years. Cereals were harvested at the beginning of heading, 50% heading and the milk stage of grain, and legumes at three stages from preflowering to full pod formation, D.M., protein and digestible D.M. yields and percentage D.M. content increased with age, whereas percentage protein content and D.M. digestibility declined. Under moisture stress conditions before and during the harvesting period D.M. yields did not increase significantly with age. Protein content of cereals under low rainfall conditions was higher than that of cereals grown in the U.K. under higher N fertilization levels. Rainfall conditions affected drastically the performance of both cereals and legumes. However, average yields were satisfactory; the barley variety 628 gave 8·98 t/ha, the highest D.M. yield among all cereal and legume varieties.

scholarly journals Nova Bosanka: New variety of winter wheat

Genetika ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Dragan Mandic ◽  
Goran Djurasinovic ◽  
Bojana Savic ◽  
Senad Kikic
Keyword(s):  
Winter Wheat ◽  
Protein Content ◽  
Seed Size ◽  
Plant Diseases ◽  
Standard Variety ◽  
Average Yield ◽  
Good Resistance ◽  
New Variety ◽  
Early Maturing ◽  
Technological Quality

Highly yielding, early maturing, excellent technological quality, resistent to lodging, optimal seed size, good resistance to plant diseases. New variety of winter wheat under the name Nova Bosanka was created by crossing the genetically divergent parents. According to overall three-year average, the variety Nova Bosanka achieved an average yield of 6.918 kg ha-1 which is 479,3 kg or 7,45% more than the values of standard variety Tina. Three-year average of protein content was 12,8%, water apsorption 59%, energy of dough 100 cm2, belongs to B1 - quality group. By the trait of resistance to lodging variety is at the level of standard.

scholarly journals Effect of NPK fertilization on the yield and yield stability of different maize genotypes

2012 ◽  
pp. 101-104
Author(s):  
Ágnes Krivián ◽  
Mihály Sárvári
Keyword(s):  
Protein Content ◽  
Starch Content ◽  
N Fertilization ◽  
Quality Parameters ◽  
Dry Period ◽  
Sowing Time ◽  
Maize Genotypes ◽  
Grain Moisture ◽  
Long Term Experiment

The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season. The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations.

Peculiarities of extraction of β-lactoglobuline in protein mineral concentrates at electroactivation of whey

2020 ◽  
Vol 2 (1) ◽  
pp. 52-68
Author(s):  
Mircea BOLOGA ◽  
Elvira VRABIE ◽  
Irina PALADII ◽  
Olga ILIASENCO ◽  
Tatiana STEPURINA ◽  
...  
Keyword(s):  
Protein Content ◽  
Whey Proteins ◽  
Sodium Dodecyl ◽  
Total Protein Content ◽  
Treatment Regimens ◽  
Sds Page ◽  
Different Types ◽  
Lower Protein ◽  
Β Lactoglobulin ◽  
Excellent Source

Introduction. Whey is a by-product and an excellent source of proteins that is rather aggressive due to a large amount of organic substances it contains. The electro-activation of whey applied in the experiments is a wasteless method that allows the va-lorification of all whey components. β-lactoglobulin (β-Lg) makes up 50% of the whey proteins and 12% of the total protein content in milk. Material and methods. The recovery of β-Lg in protein-mineral concentrates (PMC) by electro-activation processing of different types of whey with different initial protein content was investigated in seven configurations. The recovery of protein fractions in the PMCs were analyzed via electrophoresis with sodium dodecyl sulfate (SDS-PAGE) and 15% non-denaturing polyacrylamide gel (PAAG).      Results. Whey electro-fractionation and the obtaining of PMCs with predetermined protein content, namely of β-Lg, were studied on three whey types, processed at different treatment regimens and in seven configurations. The proper management of electroactivation by varying the treatment regimens will allow the electro-fractionation of different types of dairy by-products. Conclusions. The maximum amount of β-Lg recovered in PMCs on electroactivation is  66-71% depending on the processed whey and on the treatment regimens. Obviously, the extraction of β-Lg from initially lower protein content shows a higher recovery degree of β-Lg. The registered temperatures allows formation of PMCs without thermal denaturation.

scholarly journals Procedures for Breadmaking Quality Assessment of Rye Wholemeal Flour

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 331
Author(s):  
Sylwia Stępniewska ◽  
Waleed H. Hassoon ◽  
Anna Szafrańska ◽  
Grażyna Cacak-Pietrzak ◽  
Dariusz Dziki
Keyword(s):  
Protein Content ◽  
Dry Mass ◽  
Quality Parameters ◽  
Water Soluble ◽  
Rye Bread ◽  
Low Protein ◽  
Rye Flour ◽  
Flour Properties ◽  
Lower Protein

The aim of this study was to evaluate the baking value of rye flours from industrial mills and to indicate which rye flour quality parameters are the most important predictors of wholemeal rye bread quality for commercially milled rye grains. Ten wholemeal rye flours, which were characterized by ash content ranging from 1.43% to 2.42% d.m. (dry mass), were used for the study. The parameters that characterize the flour properties and the baking test were assessed. The study revealed that for the analyzed commercial rye flours, the falling number test and the amylograph properties are insufficient parameters for predicting the quality of wholemeal rye bread. The manufacture of good quality wholemeal bread requires the use of rye flour with superior quality, such as fine granulation, low protein content, low total and insoluble pentosans content, and, in particular, a high percentage of water-soluble pentosans content. Breads with a higher volume were obtained from rye flours that were generally characterized by lower protein content, lower total and insoluble pentosans content, and higher water-soluble pentosans content. Flour granulation and the percentage of water-soluble pentosans content especially, had a significant impact on bread’s hardness of crumb and the hardness of crumb’s increase during bread storage.

An analysis of seed development in Pisum sativum. XV. The influence of seed size on protein content

1991 ◽  
Vol 1 (4) ◽  
pp. 203-208 ◽  
Author(s):  
A. E. Arthur ◽  
H. Adams ◽  
K. Strouts ◽  
D. A. Jones ◽  
T. L. Wang ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Protein Content ◽  
Total Variation ◽  
Seed Development ◽  
Seed Size ◽  
Seed Weight ◽  
Genetic Component ◽  
Residual Variation ◽  
Percentage Protein

AbstractThe variation for protein content has been assessed in a series of pea (Pisum sativum L.) lines differing in mean seed size. The range of seed size within each genotype was manipulated by growing the plants in different environments and by altering the structure of the plant. The response of the lines in terms of seed weight to the environments and treatments was inconsistent, but 90% of the total variation was accounted for by differences between genotypes. In contrast, for percentage protein, the genetic component was much weaker with nearly half the variation being accounted for by within (residual) variation. The correlations between seed weight and percentage protein were highly genotype specific. For the largerseeded genotype, percentage protein increased with increasing seed size (r= +0.9, P < 0.01), while for the small-seeded genotype there was mid-range negative value (r = −0.5, P <0.01). It is suggested that any assessment of percentage protein in pea genotypes must take account of seed size.

Prospects for the utilisation of cocoyam corm meal in poultry feeding

2020 ◽  
Vol 60 (5) ◽  
pp. 603
Author(s):  
S. S. Diarra
Keyword(s):  
Starch Content ◽  
Antinutritional Factors ◽  
Feed Processing ◽  
Nutritional Profile ◽  
Diet Formulation ◽  
High Starch Content ◽  
High Starch ◽  
Poultry Feeding ◽  
Increasing Demand ◽  
The Cost

Context The increasing demand for maize for human food and industrial uses has stimulated research interest into alternative low value crops as energy sources for poultry. Several high yielding species of aroids, commonly known as taro or cocoyam, are currently in less demand for food or industrial uses and readily available for poultry feeding. Aims To review the production, nutritional profile and antinutritional factor and current uses of cocoyam corm and the effect of processing on its utilisation in poultry feeding. Key findings The nutritional profile of cocoyam with protein content comparable or higher than maize, high starch content and starch digestibility make it a suitable energy ingredient for poultry. The major issues affecting the utilisation of cocoyam include the high moisture and low dry matter contents and the presence of antinutritional factors, mainly calcium oxalate. These constraints may preclude the utilisation of cocoyam corm in commercial diets but it could be used to reduce the cost of production on small-holder farms. Conclusion With adequate processing and proper diet formulation, cocoyam corm meal may be fed up to 280 g/kg diet but unprocessed meal may exert adverse effects on poultry performance even as low as 44 g/kg. Several animal factors including age, species and type of bird (meat or egg) affect the utilisation of cocoyam corm meal by poultry. Implications Reduction of cost of animal protein production and value addition to underutilised aroids. More research is needed into animal factors and feed processing that would allow optimum utilisation of the corm by poultry.

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