Efficacy of Er:YAG laser irradiation for decontamination and its effect on biocompatibility of different titanium surfaces

Abstract Background Erbium yttrium–aluminum–garnet (Er:YAG) laser have been shown to be suitable for decontamination of titanium surfaces at a wide range of energy settings, however, high intensity of laser irradiation destroy titanium surface and low intensity cannot remove enough microbial biofilm. The aim of this study was to investigate the optimal energy setting of Er:YAG laser for decontamination of sandblasted/acid-etched (SLA) and hydroxyapatite (HA) titanium surfaces. Material and methods After supragingival biofilm construction in vivo, SLA and HA titanium discs were divided into three groups: blank control (BC, clean discs), experimental control (EC, contaminated discs) and experimental groups (EP, contaminated discs irradiated by Er:YAG laser at 40, 70, and 100 mJ/pulse). Scanning electron microscopy (SEM), live/dead bacterial fluorescent detection, and colony counting assay were used to detect the efficacy of laser decontamination. To investigate the effect of laser decontamination on titanium surface biocompatibility, MC3T3-E1 cell adhesion and proliferation activity were examined by SEM and CCK-8 assay. Results Er:YAG laser irradiation at 100 mJ/pulse removed 84.1% of bacteria from SLA titanium surface; laser irradiation at 70 and 100 mJ/pulse removed 76.4% and 77.85% of bacteria from HA titanium surface respectively. Laser irradiation improved MC3T3-E1 cell adhesion on both titanium surfaces. For SLA titanium discs, 100 mJ/pulse group displayed excellent cellular proliferation activity higher than that in BC group (P < 0.01). For HA titanium discs, 70 mJ/pulse group showed the highest activity comparable to BC group (P > 0.05). Conclusions With regards to efficient microbial biofilm decontamination and biocompatibility maintenance, Er:YAG laser at 100 mJ/pulse and 70 mJ/pulse are considered as the optimal energy settings for SLA titanium and HA titanium surface respectively. This study provides theoretical basis for the clinical application of Er:YAG laser in the treatment of peri-implantitis.

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Physicochemical Changes of Contaminated Titanium Discs Treated With Erbium-Doped Yttrium Aluminum Garnet (Er:YAG) Laser Irradiation or Air-Flow Abrasion: An In Vitro Study

Journal of lasers in medical sciences ◽

10.34172/jlms.2021.67 ◽

2021 ◽

Vol 12 (1) ◽

pp. e67-e67

Author(s):

Reza Amid ◽

Mahdi Kadkhodazadeh ◽

Seyed Massoud Mojahedi ◽

Maedeh Gilvari Sarshari ◽

Zeinab Zamani

Keyword(s):

Laser Irradiation ◽

Yttrium Aluminum Garnet ◽

Er:Yag Laser ◽

Air Flow ◽

Positive Control ◽

Control Group ◽

Weight Percentage ◽

Erbium Doped ◽

Titanium Surfaces ◽

The Mean

Introduction: Peri-implantitis is a common complication of dental implant treatment. A cause-and-effect relationship has been previously documented between microbial plaque and peri-implantitis and implant failure. A difference has been reported in the disinfection efficacy of erbium laser irradiation and air-flow abrasion for contaminated titanium surfaces. Also, the surface changes caused by lasers and air-flow abrasion have not been well studied. Thus, the purpose of this study was to compare the surface changes of contaminated titanium discs following decontamination by erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation and air-flow abrasion. Methods: Twenty-eight intact, sandblasted, and acid-etched (SLA) titanium discs were used. Twenty-four titanium discs were contaminated with Escherichia coli. Then, they were decontaminated by using Er:YAG laser irradiation and air-flow abrasion. Four discs remained intact. The mean and standard deviation of the contact angle and the weight percentage of aluminum, titanium, oxygen, carbon, phosphorus, and calcium were measured. Qualitative changes in surface topography of titanium discs were assessed by scanning electron microscopy (SEM). Results: The mean weight percentage of carbon in the air-flow abrasion group (4.98%) experienced a significant reduction compared with the contaminated (positive control) group (P=0.035). The contact angles were 46.54° and 38.67° in the laser and air-flow abrasion groups respectively, which were significantly lower than the value in the positive control group (75.15°) (P ≤0.001). SEM micrographs showed no significant change in the surface area in either technique. Conclusion: Air-flow abrasion was more successful in improving the surface characteristics of titanium discs with no alteration in surface topography or elements, compared with Er:YAG laser irradiation. Further studies regarding the safety of the Er:YAG laser for the decontamination of titanium surfaces are recommended.

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Fibroblasts Adhesion to Laser-Modified Titanium Surfaces—A Systematic Review

Materials ◽

10.3390/ma14237305 ◽

2021 ◽

Vol 14 (23) ◽

pp. 7305

Author(s):

Julia Kensy ◽

Maciej Dobrzyński ◽

Rafał Wiench ◽

Kinga Grzech-Leśniak ◽

Jacek Matys

Keyword(s):

Systematic Review ◽

Cell Adhesion ◽

Energy Density ◽

Titanium Surface ◽

Laser Energy ◽

Included Study ◽

Laser Application ◽

Laser Parameters ◽

Titanium Surfaces ◽

Fibroblast Adhesion

Objective: Laser treatment has been recently introduced in many fields of implant dentistry. The systematic review tried to address the question: “How does laser modification of titanium surface influence fibroblast adhesion?”. Methods: An electronic search of the PubMed and Scopus databases was performed. The following keywords were used: (laser) AND (fibroblast) AND (titanium) AND (implant OR disc) AND (proliferation OR adhesion). Initially, 136 studies were found. Ten studies met the inclusion criteria and were included in the review. All studies chosen to be included in the review were considered to have a low risk of bias. Results: Studies included in the review varied with laser parameters or ways of observing fibroblast behavior. Studies showed that fibroblasts tend to take different shapes and create extensions on modified surfaces and that their metabolic activity is more intense. One study concentrated on laser application and showed that three-directional laser application is the most successful in terms of fibroblast adhesion. Studies which concentrated more on laser parameters showed that too low energy density (lower or equal to 0.75 J/cm2) does not influence fibroblast adhesion. Increasing the energy density over 0.75 J/cm2 causes better cell adhesion of fibroblasts to the laser-modified sample. One included study focused on increasing titanium surface wettability, which also positively influenced cell adhesion. Conclusion: The studies included in the review proved a positive effect of laser-modified titanium surfaces on fibroblast adhesion. However, the application of an appropriate laser energy dose is crucial.

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Studies on enhancement of biofilm formation and adherence due to mechanical treatment of titanium surfaces in cooling-water systems

Biofilms ◽

10.1017/s1479050508002226 ◽

2008 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

R. P. George ◽

J. Gopal ◽

P. Muraleedharan ◽

B. Anandkumar ◽

R. Baskaran ◽

...

Keyword(s):

Biofilm Formation ◽

Power Plants ◽

Mechanical Treatment ◽

Titanium Surface ◽

Sea Water ◽

Long Term Effects ◽

Wide Range ◽

Titanium Surfaces ◽

Mechanical Cleaning

ABSTRACTTitanium has proven to be the heat exchanger material of choice for sea-water-cooled power plants owing to its outstanding resistance to pitting and crevice corrosion in a wide range of aggressive media. However, the inertness of the titanium surface makes it highly susceptible to biofilm formation and subsequent biofouling. This can hinder the heat transfer properties and flow of water. Fouling control strategies in condensers include a combination of mechanical, chemical and thermal treatments. However, reports from various industrial situations suggest that mechanical treatment may not have long-term effects. This study aimed to find out whether mechanical cleaning eventually enhances biofilm formation and increases the adherence of biofilm. In our studies epifluorescence micrographs of biofilms on control and mechanically treated titanium surfaces clearly showed accelerated biofilm formation as well as increased adherence on the mechanically cleaned surface. Total counts of viable bacteria acquired by culturing technique, and biofilm thickness measurements made using microscopic techniques, confirmed this observation. Surface profilometry showed increased roughness of the titanium surface, facilitating adherence of biofilm. The number of microbial species was higher on mechanically cleaned and re-exposed surfaces than on fresh titanium. Thus we concluded that mechanical cleaning can increase biofilm formation and adherence of biofilm, thereby increasing the potential of biofouling in the long term.

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Effects of Er:YAG laser irradiation of different titanium surfaces on osteoblast response

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-021-06493-y ◽

2021 ◽

Vol 32 (3) ◽

Author(s):

Christian Wehner ◽

Markus Laky ◽

Hassan Ali Shokoohi-Tabrizi ◽

Christian Behm ◽

Andreas Moritz ◽

...

Keyword(s):

Gene Expression ◽

Laser Irradiation ◽

Titanium Surface ◽

Collagen Type ◽

Er:Yag Laser ◽

Rough Surfaces ◽

Clinical Treatment ◽

Enzyme Linked Immunosorbent Assay ◽

Collagen Type 1

AbstractThe aim of this in vitro study was to evaluate the effects of erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation on titanium surface topography and the proliferation and differentiation of osteoblasts using standard clinical treatment settings. Er:YAG laser irradiation at two levels ((1): 160 mJ, pulse at 20 Hz; (2): 80 mJ, pulse at 20 Hz) was applied to moderately rough and smooth titanium disks before MG-63 osteoblast-like cells were cultured on these surfaces. Titanium surface and cell morphology were observed by scanning electron microscopy. Cell proliferation/viability was measured by CCK-8 test. Gene expression of alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL), and collagen type 1 was measured by qPCR, and OPG and OC protein production was determined by enzyme-linked immunosorbent assay. Treatment with Er:YAG laser at 160 mJ/20 Hz markedly caused heat-induced fusion of titanium and cell condensation on moderately rough surfaces, but not in smooth surfaces. MG-63 proliferation/viability decreased after 5 days in moderately rough surfaces. The expression of ALP, OC, OPG, and collagen type 1 was unaffected by laser treatment at 160 mJ/20. Laser irradiation at 80 mJ/20 Hz enhanced RANKL gene expression after 5 days in moderately rough surfaces. Study results suggest that Er:YAG laser irradiation at clinically relevant setting has no essential effect on osteogenic gene and protein expression of osteoblasts. However, surface structure, cell attachment, and proliferation are influenced by both treatment protocols, which implies that caution should be taken in the clinical treatment of peri-implant diseases when Er:YAG laser is used.

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The influence of Er:YAG laser irradiation on re-bonding shear bond strength of enamel

Journal of Adhesion Science and Technology ◽

10.1080/01694243.2021.1888581 ◽

2021 ◽

pp. 1-19

Author(s):

Huimin Fang ◽

Caitian Teng ◽

Pengfei Lu ◽

Yang Cao ◽

Qing Yu

Keyword(s):

Bond Strength ◽

Laser Irradiation ◽

Shear Bond Strength ◽

Er:Yag Laser

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Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection

Nature Communications ◽

10.1038/s41467-021-23069-0 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Shuyi Wu ◽

Jianmeng Xu ◽

Leiyan Zou ◽

Shulu Luo ◽

Run Yao ◽

...

Keyword(s):

Dental Implant ◽

Pathogenic Bacteria ◽

Titanium Surface ◽

Polymeric Coating ◽

Antibacterial Efficacy ◽

Polymeric Coatings ◽

Implant Infection ◽

Titanium Surfaces

AbstractPeri-implant infection is one of the biggest threats to the success of dental implant. Existing coatings on titanium surfaces exhibit rapid decrease in antibacterial efficacy, which is difficult to promisingly prevent peri-implant infection. Herein, we report an N-halamine polymeric coating on titanium surface that simultaneously has long-lasting renewable antibacterial efficacy with good stability and biocompatibility. Our coating is powerfully biocidal against both main pathogenic bacteria of peri-implant infection and complex bacteria from peri-implantitis patients. More importantly, its antibacterial efficacy can persist for a long term (e.g., 12~16 weeks) in vitro, in animal model, and even in human oral cavity, which generally covers the whole formation process of osseointegrated interface. Furthermore, after consumption, it can regain its antibacterial ability by facile rechlorination, highlighting a valuable concept of renewable antibacterial coating in dental implant. These findings indicate an appealing application prospect for prevention and treatment of peri-implant infection.

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