The prevalence of human herpesvirus 8 in normal, premalignant, and malignant cervical samples of Iranian women

Abstract Background Regard to this fact that the main transmission route of HPV and HHV-8 is via sexual activity, it is reasonable to speculate that coinfection of HPV and HHV-8 may have been played an important role in the development of cervical cancer. The aim of this study was to estimate the prevalence of HHV-8 and the frequency of HPV and HHV-8 coinfection in cervical samples of patients with cervical cancer and healthy individuals. Methods In total, 364 samples from 61 patients with cervical cancer, 124 women with premalignant lesions, and 179 healthy individuals were investigated by nested-PCR. Results The frequency of HHV-8 was found to be 22.9%, 17.7%, and 14.5% in cervical cancer, premalignant lesions, and normal specimens, respectively (P = 0.308). The overall prevalence of coinfection between HHV-8 and HPV was shown to be 16.2%. The HPV prevalence was higher in HHV-8 positive samples than HHV-8 negative specimens in all three studied groups and this difference was reached a statistically significant level (P = 0.002). However, no significant differences were found between HHV-8 positivity and HPV genotypes (P = 0.08). Conclusions Our results showed the higher rate of HHV-8 genome detection in cervical cancer group than control group. However, future studies with larger sample sizes and evaluation of expression of HHV-8 proteins are warranted.

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Neutrophil-to-Lymphocyte Ratio and Platelet-to-Lymphocyte Ratio in Blood to Distinguish Lung Cancer Patients from Healthy Subjects

Disease Markers ◽

10.1155/2020/8844698 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Xuming Zhu ◽

Huizhu Song ◽

Yan Chen ◽

Feifei Han ◽

Qiong Wang ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Patients ◽

Healthy Subjects ◽

Control Group ◽

Healthy Individuals ◽

Platelet To Lymphocyte Ratio ◽

Lung Cancer Patients ◽

Lymphocyte Ratio ◽

Cancer Group ◽

Level Group

Objective. Inflammation-driven markers play a crucial role in tumorigenesis and tumor progression. The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in blood are systemic inflammatory response markers. Some reports have showed that NLR and PLR are related to a poor prognosis in patients with lung cancer. However, little studies have reported whether NLR and PLR can be diagnostic markers for lung cancer. The aim of the current study is to investigate the roles of NLR and PLR in diagnosing lung cancer. Methods. This study analyzed data from lung cancer patients and healthy individuals in Wuxi People’s Hospital Affiliated with Nanjing Medical University. The Mann–Whitney U test was performed to compare differences between the lung cancer group and the control group. Based on white blood cell (WBC) counts, both lung cancer patients and healthy individuals were divided into the low-level group, moderate-level group, and high-level group. The Kruskal-Wallis test was applied to compare differences of NLR and PLR among those groups with different WBC counts. Spearman correlation analysis was used to assess correlations. Receiver operating characteristic (ROC) curves were performed to determine diagnostic accuracy. Results. 210 patients diagnosed with lung cancer and 261 healthy subjects were enrolled in this study. Levels of NLR and PLR increased in the lung cancer group compared with the control group ( P < 0.001 ). For the lung cancer group, NLR levels could rise with the increasing of WBC levels ( P < 0.001 ) while PLR levels had no significant variation with the increasing of WBC levels ( P = 0.206 ). For the control group, NLR levels could rise with the increasing of WBC levels ( P < 0.001 ) while PLR levels would decline with the increasing of WBC levels ( P < 0.001 ). In the lung cancer group, both NLR and PLR had no significant correlations with aspartate transaminase, urea, and glucose. The area under the curve (AUC) with 95% confidence interval (95% CI) of NLR and PLR to distinguish lung cancer patients from healthy subjects was, respectively, 0.684 (0.634-0.735) and 0.623 (0.571-0.674). When NLR and PLR were combined, AUC (95% CI) increased to 0.691 (0.642-0.740). Conclusions. NLR and PLR alone have moderate ability to distinguish lung cancer patients from healthy subjects. Furthermore, combination forms of NLR and PLR can improve diagnostic ability.

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Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy

Biomolecules ◽

10.3390/biom11111691 ◽

2021 ◽

Vol 11 (11) ◽

pp. 1691

Author(s):

Oyeon Cho ◽

Do-Wan Kim ◽

Jae-Youn Cheong

Keyword(s):

Cervical Cancer ◽

Pathway Analysis ◽

Cancer Diagnosis ◽

Healthy Individuals ◽

Cancer Group ◽

Before And After ◽

Primary Chemoradiotherapy ◽

Preliminary Study ◽

Non Coding Rnas ◽

Exosomal Rnas

This preliminary study aimed to screen non-coding RNAs (ncRNAs) from plasma exosomes as a new method for cervical cancer diagnosis. Differentially expressed RNAs were initially selected from among a group of 12 healthy individuals (normal group) and a pretreatment group of 30 patients with cervical cancer (cancer group). Then, we analyzed the association between an ncRNA-mRNA network and cancer using ingenuity pathway analysis after secondary selection according to the number and correlation of mRNAs (or ncRNAs) relative to changes in the expression of primarily selected ncRNAs (or mRNAs) before and after chemoradiotherapy. The number of RNAs selected from the initial RNAs was one from 13 miRNAs, four from 42 piRNAs, four from 28 lncRNAs, nine from 18 snoRNAs, 10 from 76 snRNAs, nine from 474 tRNAs, nine from 64 yRNAs, and five from 67 mRNAs. The combination of miRNA (miR-142-3p), mRNAs (CXCL5, KIF2A, RGS18, APL6IP5, and DAPP1), and snoRNAs (SNORD17, SCARNA12, SNORA6, SNORA12, SCRNA1, SNORD97, SNORD62, and SNORD38A) clearly distinguished the normal samples from the cancer group samples. We present a method for efficiently screening eight classes of RNAs isolated from exosomes for cervical cancer diagnosis using mRNAs (or ncRNAs) altered by chemoradiotherapy.

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POLYMORPHISM ARG72PRO OF THE P53 GENE AND THE RISK OF HUMAN PAPILLOMAVIRUS ASSOCIATED CERVICAL CANCER IN WOMEN FROM KYRGYZ REPUBLIC

Problems in oncology ◽

10.37469/0507-3758-2017-63-5-748-752 ◽

2017 ◽

Vol 63 (5) ◽

pp. 748-752

Author(s):

Zhaynagul Isakova ◽

Elnura Talaybekova ◽

Kyyal Makieva ◽

N. Bukuev ◽

D. Asambaeva ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

P53 Gene ◽

Hiv Positive ◽

Control Group ◽

Genotype Distribution ◽

Kyrgyz Republic ◽

Cancer Group ◽

Significant Difference ◽

Dna Types

Aim. Allele frequency and genotype distribution in the cervical cancer group were compared with those of the control group to determine whether polymorphism Arg72Pro of Р53 gene elevates the susceptibility of Kyrgyz women to cervical cancer (CC). Materials and Methods. A total of 102 women (mean age 53,5±10 years) of Kyrgyz nationality with cervical cancer were recruited into the study and 102 healthy women were used as the control group (mean age 46,5±8,5 years). The diagnosis of cervical cancer was confirmed histologically. 88 % (90/102) of women with cervical cancer were human papillomavirus (HPV) positive. 17 % (15/90) specimens were positive for HPV type 16, 48 % (43/90) were positive for HPV type 18 and 35 % (32/90) were positive both HPV - 16/18. Presence of human papillomavirus DNA types 16 and 18 was analyzed by polymerase chain reaction with hybridization-fluorescence detection. The Arg72Pro polymorphisms of the P53 gene were determined by the PCR-RFLP method. Results. No significant difference was found between genotype distributions in the cervical cancer patients and the control group (χ2=1,24; р=0,54). However, the Arg72Arg genotype and Arg72 allele was significantly more frequent in women with cervical cancers infected with HPV than in the control group (χ2=7,25; р=0,027 for для genotype, χ2=6,83; р=0,009 for allele). Women who are HIV positive and having the Arg72 allele had 1,94 fold (OR=1,94 [1,20-3,15]; р=0,009) higher risk of developing CC compared with subjects carrying neither of these alleles. HPV positive women carrying the genotype Arg72Arg had 1,85 fold (OR=1,85 [1,03-3,32]; р=0,027) higher risk of CC. Conclusion. The Arg72Arg genotype of р53 gene in HIV positive women from Kyrgyz Republic may represent a potential risk factor for the development of cervical cancer.

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The association of TNF-α promoter polymorphisms with genetic susceptibility to cervical cancer in a Chinese Han population

10.21203/rs.3.rs-378408/v1 ◽

2021 ◽

Author(s):

Jia Yang ◽

Yingying Wang ◽

Zhiling Yan ◽

Chuanyin Li ◽

Shuyuan Liu ◽

...

Keyword(s):

Cervical Cancer ◽

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Chinese Han Population ◽

Control Group ◽

Chinese Han ◽

Han Population ◽

Cancer Group ◽

Tnf Α

Abstract Background The Tumour necrosis factor-α (TNF-α) gene plays an important role in the host immune response. Recent studies have found that TNF-α also plays an important role in cancer. Polymorphism of the TNF-α promoter region is considered to influence its transcription and be a risk factor for tumorigenesis. In the current study, we evaluated the role of TNF-α promoter region polymorphisms in susceptibility to cervical intraepithelial neoplasia (CIN) and cervical cancer (CC). Methods A total of 2,732 subjects, including 1,173 healthy controls, 579 patients with CIN and 980 patients with cervical cancer in a Chinese Han population, were selected for the current study. Five SNPs in the TNF-α promoter, rs1799964 (C > T), rs800630 (A > C), rs1799724 (C > T), rs1800629 (A > G) and rs361525 (A > G), were selected and genotyped using TaqMan Assays. The association of these SNPs with cervical cancer was evaluated among healthy controls and CIN and cervical cancer patients. Results The frequency distribution of rs1800629 and rs361525 alleles was significantly different between the cervical cancer group and the control group (P = 0.009 and P = 0.002). The A alleles of rs1800629 and rs361525 were found to be a protective factor (OR = 0.722; 95%CI = 0.564–0.923) and a risk factor (OR = 1.693; 95%CI = 1.205–2.378) for cervical cancer, respectively. In comparison of different pathological types of cervical cancer group and the control group, the distribution of allele frequencies of rs1800629 and rs361525 was significantly different between the squamous cell carcinoma and control groups (P = 0.002 and P < 0.001). The A alleles of rs1800629 and rs361525 were protective (OR = 0.659; 95%CI = 0.502–0.864) and risk (OR = 1.868; 95%CI = 1.317–2.648) factors for cervical squamous cell carcinoma, respectively. Conclusion rs1800629 and rs361525 in the TNF-α promoter are associated with susceptibility to cervical cancer and squamous cell carcinoma in the Chinese Han population.

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HPV prevalence and type-distribution in cervical cancer and premalignant lesions of the cervix: A population-based study from Northern Ireland

Journal of Medical Virology ◽

10.1002/jmv.24447 ◽

2016 ◽

Vol 88 (7) ◽

pp. 1262-1270 ◽

Cited By ~ 13

Author(s):

Lesley A. Anderson ◽

Michael A. O'Rorke ◽

Robbie Wilson ◽

Jackie Jamison ◽

Anna T. Gavin ◽

...

Keyword(s):

Cervical Cancer ◽

Northern Ireland ◽

Population Based ◽

Premalignant Lesions ◽

Population Based Study ◽

Type Distribution ◽

Hpv Prevalence

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The effects of shoulder and scapular region rigid taping and kinesio taping on scapular dyskinesis and pectoralis minor shortness in overhead athletes

Orthopaedic Journal of Sports Medicine ◽

10.1177/2325967117s00086 ◽

2017 ◽

Vol 5 (2_suppl2) ◽

pp. 2325967117S0008

Author(s):

Sibel Tekeli Özer ◽

Damla Gülpınar ◽

Sevgi Sevi Yeşilyaprak

Keyword(s):

Demographic Data ◽

Control Group ◽

Scapular Dyskinesis ◽

Future Studies ◽

Overhead Athletes ◽

Positive Effects ◽

Kinesio Taping ◽

Pectoralis Minor ◽

Larger Sample ◽

Scapular Region

Objective: The aim of the study was to investigate the effects of shoulder and scapular region rigid taping and kinesio taping on scapular dyskinesis and pectoralis minor shortness in overhead athletes. Method: This study included 72 overhead athletes. The athletes were randomly divided into four groups: kinesio taping (shoulder and scapular region) was applied to Kinesio Taping Group (KB), rigid taping (shoulder and scapular region) was applied to Rigid Taping Group (RB), placebo kinesio taping was applied to Placebo Taping Group (PB). Control group received no tape. Demographic data of overhead athletes, anthropometric characteristics, pain severity and injury background were evaluated before taping. Pectoralis minor shortness (Pectoralis Minor Index) and scapular dyskinesis (Scapular Dyskinesis Test) were evaluated before taping, immediately after (30-minutes) taping and after 48 hours of use within 12-24 hours. Control group was evaluated at the same periods. Results: Before taping; demographic, anthropometric and sports-related characteristics were similar in groups and there was no statistically difference (p>0.05). It was determined that Pectoralis Minor Index (PMI) and scapular dyskinesis improved immediately after taping and after 48 hours of use in KB and RB (p<0.05). There were no significant differences after taping for PMI among groups (p>0.05). Conclusion: Kinesio taping and rigid taping seems to have positive effects on scapular dyskinesis and pectoralis minor shortness in overhead athletes. Future studies on larger sample size are needed to verify the differences of the effects of these taping techniques between placebo or controls.

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Assessment of the Haematological Alterations in Cervical Cancer Patients Attending Sabratha

Clinical Oncology Research and Reports ◽

10.31579/2693-4787/036 ◽

2022 ◽

Vol 3 (1) ◽

pp. 01-09

Author(s):

Azab Elsayed Azab ◽

Fikry Ali Abushofa ◽

Hajar Musa Al Ghawi

Keyword(s):

Cervical Cancer ◽

Cancer Patients ◽

National Cancer Institute ◽

Complete Blood Count ◽

Statistical Significance ◽

Venous Blood ◽

Cervix Cancer ◽

Haematological Parameters ◽

Control Group ◽

Healthy Individuals

Background: Cervix cancer is one of the most common cancers in women worldwide, and is the third most common malignant disease in women. It is one of the main health problems in Libyan women. Blood act as a pathological reflector of the status of exposed patient to infections and other conditions. Laboratory tests on the blood are vital tools that help detect any deviation from normal in the human body As the disease progresses, changes appear in haematological parameters which have been of relevant consideration in context of cancer patients. Objectives: The present study aimed to evaluate the alterations in haematological parameters among cervical cancer patients in Sabratha National Cancer Institute. Subjects and Methods: The present study was conducted on 150 cervical cancer patients, attending the National Cancer Institute of Sabratha from the 11th February, 2006 to the 3rd February, 2020. This study was approved by the Research and Ethical Committee of Sabratha University and Sabratha National Cancer Institute. Age was extracted from patient files. Also, 60 healthy individuals without any chronic disease were recruited for the control group. Blood samples were collected by vein puncture, 3 ml of venous blood withdrawn from each participant in the study by using disposable syringes under aseptic technique; they then transferred to a sterile EDTA tube, for complete blood count. The statistical significance of differences between groups was evaluated with the Mann Whitney U test. Results: The results showed that the mean age of the cervix cancer patients was 53.37±11.6 years.RBCs count, hemoglobin concentration, Hct value, MCH, MCHC, and lymphocytes % were significantly (P<0.01) decreased compared with the healthy individuals. On the other hand, leukocytes and platelets count, mixed %, neutrophils %, PLTs/Lymph, and Neutrophils/Lymphocytes ratios were significantly increased as compared with the healthy individuals. Conclusion: It can be concluded that a significant increase in leukocytes and platelets count, mixed %, neutrophils %, and the studied inflammation related haematological parameters and a significant decreased in lymphocytes %, RBCs count and most its indices. Further haematological studies are needed to confirm these results. Also, there is need to routinely monitor the haematological parameters and among cervical cancer patients.

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Adiponectin Level in Women with Breast Cancer

Asian Pacific Journal of Cancer Biology ◽

10.31557/apjcb.2017.2.4.91-94 ◽

2017 ◽

Vol 2 (4) ◽

pp. 91-94

Author(s):

Fatemeh Homaee ◽

Kamran Ghaffarzadehgan ◽

Azar Aziminia ◽

Hamideh Ghodrati ◽

Azra Izanloo ◽

...

Keyword(s):

Breast Cancer ◽

Adiponectin Level ◽

Breast Cancer Incidence ◽

Control Group ◽

Future Studies ◽

Cancer Group ◽

Breast Cancer Group ◽

Lower Adiponectin Level ◽

Positive Effect ◽

Healthy Females

Introduction: Adiponectin is one of the plasma protein derived from adipose tissue which has a positive effect on the metabolism of fat and lipid, resulting from its own receptors. Increase in body mass index (BMI) has a direct relationship with life length. Obesity has effects on secretion of some hormones related to adipokines. Adiponectin is an adipokine having an opposite relationship with insulin resistance and breast cancer. There is also an association between breast cancer incidence and obesity. Adiponectin level decreases in women affected by breast cancer and knowing this fact can help treat and prevent the cancer by giving adiponectin supplements. The present study compared the adiponectin levels in women affected by breast cancer and healthy females as a control group. Material and Method: In this study, 80 women with breast cancer and 80 healthy females as controls were selected and adiponectin level was compared between them. Result: Breast cancer group had higher BMI mean (28.77 ± 6.05) and lower adiponectin level in comparison to control group (p= 0.0001). Conclusion: Considering our findings, breast cancer risk is higher in women with higher BMI. Therefore, informing women in this regard could have a great effect on preventing this wide-spread disease. Taking adiponectin supplements as pills or injection could be a preventing step for breast cancer, which needs to be further cultivated in future studies.

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