scholarly journals Screening Plasma Exosomal RNAs as Diagnostic Markers for Cervical Cancer: An Analysis of Patients Who Underwent Primary Chemoradiotherapy

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1691
Author(s):  
Oyeon Cho ◽  
Do-Wan Kim ◽  
Jae-Youn Cheong
Keyword(s):  
Cervical Cancer ◽  
Pathway Analysis ◽  
Cancer Diagnosis ◽  
Healthy Individuals ◽  
Cancer Group ◽  
Before And After ◽  
Primary Chemoradiotherapy ◽  
Preliminary Study ◽  
Non Coding Rnas ◽  
Exosomal Rnas

This preliminary study aimed to screen non-coding RNAs (ncRNAs) from plasma exosomes as a new method for cervical cancer diagnosis. Differentially expressed RNAs were initially selected from among a group of 12 healthy individuals (normal group) and a pretreatment group of 30 patients with cervical cancer (cancer group). Then, we analyzed the association between an ncRNA-mRNA network and cancer using ingenuity pathway analysis after secondary selection according to the number and correlation of mRNAs (or ncRNAs) relative to changes in the expression of primarily selected ncRNAs (or mRNAs) before and after chemoradiotherapy. The number of RNAs selected from the initial RNAs was one from 13 miRNAs, four from 42 piRNAs, four from 28 lncRNAs, nine from 18 snoRNAs, 10 from 76 snRNAs, nine from 474 tRNAs, nine from 64 yRNAs, and five from 67 mRNAs. The combination of miRNA (miR-142-3p), mRNAs (CXCL5, KIF2A, RGS18, APL6IP5, and DAPP1), and snoRNAs (SNORD17, SCARNA12, SNORA6, SNORA12, SCRNA1, SNORD97, SNORD62, and SNORD38A) clearly distinguished the normal samples from the cancer group samples. We present a method for efficiently screening eight classes of RNAs isolated from exosomes for cervical cancer diagnosis using mRNAs (or ncRNAs) altered by chemoradiotherapy.

scholarly journals Survival trend after invasive cervical cancer diagnosis in sweden before and after cytologic screening. 1960–1984

Cancer ◽  
1994 ◽  
Vol 73 (1) ◽  
pp. 140-147 ◽  
Author(s):  
Hans-Olov Adami ◽  
Jan Pontén ◽  
PÄR Sparén ◽  
Reinhold Bergström ◽  
Leif Gustafsson ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Diagnosis ◽  
Invasive Cervical Cancer ◽  
Before And After

scholarly journals The prevalence of human herpesvirus 8 in normal, premalignant, and malignant cervical samples of Iranian women

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Sara Chavoshpour-Mamaghani ◽  
Zabihollah Shoja ◽  
Yaghoub Mollaei-Kandelous ◽  
Kimia Sharifian ◽  
Somayeh Jalilvand
Keyword(s):  
Cervical Cancer ◽  
Human Herpesvirus ◽  
Premalignant Lesions ◽  
Control Group ◽  
Healthy Individuals ◽  
Herpesvirus 8 ◽  
Future Studies ◽  
Cancer Group ◽  
Hpv Prevalence ◽  
Larger Sample

Abstract Background Regard to this fact that the main transmission route of HPV and HHV-8 is via sexual activity, it is reasonable to speculate that coinfection of HPV and HHV-8 may have been played an important role in the development of cervical cancer. The aim of this study was to estimate the prevalence of HHV-8 and the frequency of HPV and HHV-8 coinfection in cervical samples of patients with cervical cancer and healthy individuals. Methods In total, 364 samples from 61 patients with cervical cancer, 124 women with premalignant lesions, and 179 healthy individuals were investigated by nested-PCR. Results The frequency of HHV-8 was found to be 22.9%, 17.7%, and 14.5% in cervical cancer, premalignant lesions, and normal specimens, respectively (P = 0.308). The overall prevalence of coinfection between HHV-8 and HPV was shown to be 16.2%. The HPV prevalence was higher in HHV-8 positive samples than HHV-8 negative specimens in all three studied groups and this difference was reached a statistically significant level (P = 0.002). However, no significant differences were found between HHV-8 positivity and HPV genotypes (P = 0.08). Conclusions Our results showed the higher rate of HHV-8 genome detection in cervical cancer group than control group. However, future studies with larger sample sizes and evaluation of expression of HHV-8 proteins are warranted.

scholarly journals Pengaruh Psikokuratif terhadap Kadar VEGF-c, Kortisol dan Skor HADS Pasien Kanker Serviks Stadium Lanjut

2020 ◽  
Vol 7 (1) ◽  
pp. 58
Author(s):  
Soetrisno Soetrisno ◽  
Sri Sulistyowati ◽  
Rubin Enhui Tjiang ◽  
Hari Wujoso ◽  
Hafi Nurinasari ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Diagnosis ◽  
Intervention Group ◽  
Advanced Stage ◽  
Advanced Cervical Cancer ◽  
Group Design ◽  
Intervention Method ◽  
Before And After ◽  
The Mean ◽  
Cancer Diagnosis And Therapy

Background: Cancer diagnosis and therapy that causes chronic stress, its progression to depression  increase cortisol and VEGF-c levels in advanced cervical cancer patientsObjective: To  know the VEGF-c, cortisol and HADS level  in advanced stage cervical cancer patients who get Psychocurative intervention Method : An experimental study of pretest – posttest controle group design in outpatient oncology clinic and ward of Doctor Moewardi General Hospital. Thirty subjects with advanced cervical cancer were randomly divided into 15 patients in the intervention group, and 15 in control. Psycho-curative intervention 4 times a month, 1 time a week, 60 minutes duration. Before and after intervention, each subject of the two groups was examined levels of VEGF-c, cortisol and HADS scores. Data obtained were analyzed by independent t test, Mann Whitney, Pair t and Wilcoxon with the 19th version of SPSS for Windows.Result and Discussion: Average VEGF-c levels before intervention 9006.53 ± 2181.49, after intervention 5631.20 ± 2071.55, p <0.001. The mean cortisol level before intervention was 12.29 ± 4.36, after intervention 6.71 ± 3.88, p <0.001. The mean HADS Anxiety score before intervention was 14.13 ± 3.02, after intervention 8.47 ± 3.07, p <0.001. Mean Depression HADS scores before intervention 13.80 ± 3.21, after intervention 7.20 ± 2.70, p <0.001.Conclusion : VEGF-c levels, cortisol, HADS anxiety scores and depression in patients with advanced cervical cancer decline after psychocurative intervention. Keywords : Psychocurative; VEGF-c; Cortisol; HADS; Cervical cancer

Visfatin concentration and anthropometric/biochemical parameters in healthy individuals-preliminary study

2018 ◽  
Author(s):  
Justyna Nowak ◽  
Karolina Kulik-Kupka ◽  
Joanna Kowalska ◽  
Iwona Zielen-Zynek ◽  
Bartosz Hudzik ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Healthy Individuals ◽  
Preliminary Study

scholarly journals A preliminary study on diabetes self-management education and glycemic control among patients with diabetes mellitus

2019 ◽  
Vol 9 (9) ◽  
pp. 98 ◽  
Author(s):  
Kisokanth G. ◽  
Indrakumar J. ◽  
Prathapan S. ◽  
Joseph J. ◽  
Ilankoon I.M.P.S.
Keyword(s):  
Diabetes Mellitus ◽  
Glycemic Control ◽  
Management Education ◽  
Self Management ◽  
Rank Test ◽  
Diabetic Patients ◽  
Effective Measure ◽  
Before And After ◽  
Patients With Diabetes ◽  
Preliminary Study

This study was aimed to assess the effectiveness of diabetes self-management education (DSME) in the improvement of glycemic control among patients with type 2 Diabetes Mellitus (T2DM) in Batticaloa District, Sri Lanka. The study was a prospective interventional study and conducted as a preliminary study at medical clinic, Base hospital, Kaluwanchikudy, Batticaloa. Thirty patients with T2DM were included based on inclusion and exclusion criteria. A structured individual diabetes self-management education for 10 hours (one hour per week) was delivered to diabetic patients by the trained Nurse Health Educator. Glycosylate hemoglobin (HbA1c) was assessed as a main outcome measure and Fasting Blood Sugar (FBS), Body Mass Index (BMI) of each patient were also measured and recorded before and after the intervention. The respondent rate was 96.7% (n = 29). Majority of them were females (n = 25, 86.2%). A Wilcoxon signed rank test showed that DSME had a statistically significant reduction in HbA1c [8.60 (IQR 2.60) vs. 7.40 (IQR 2.10), p = .000] and FBS level [159.00 (IQR 77.50) vs. 134.00 (IQR 40.50), p = .002] at 3 months of intervention. The mean BMI at baseline was higher compared to 3 months of intervention [24.88 (SD ± 3.06) vs. 24.19 (SD ± 2.79)] which was statistically significant (p = .000). Majority of participants (n = 22, 75.9%) had improved their HbA1c level by ≥ 0.5% in 3 months. The diabetes self-management education is an effective measure in improving glycemic control and other clinical parameters among patients with T2DM. Thus, DSME needs to be implemented among clinic patients with T2DM for the better outcome and the preventions of complications.

scholarly journals Changes of Walking Activity During the First Cycle Phases of COVID-19 Pandemic: A Case Study of Seoul, Korea

2021 ◽  
pp. 193758672110265
Author(s):  
Youngjun Park ◽  
Haekwon Chung ◽  
Sohyun Park
Keyword(s):  
Public Health ◽  
Physical Inactivity ◽  
Voluntary Participation ◽  
Walking Activity ◽  
Significant Difference ◽  
Before And After ◽  
Acceleration And Deceleration ◽  
Preliminary Study ◽  
The Government

Aim: This study explores the changes in regular walking activities during the phases of the pandemic. Background: With the spread of COVID-19 transmission, people are refraining from going out, reducing their physical activity. In South Korea, COVID-19 broke out in the 4th week of 2020 and experienced the first cycle phases of the pandemic, such as outbreak, widespread, and decline. In response to the pandemic, the government encouraged voluntary participation in social distancing campaigns, and people reduced their outside activities. Methods: This article examines the decrease and increase of the Prevalence of Regular Walking (≥30 min of moderate walking a day, on ≥5 days a week) by the COVID-19 phases. This study is based on weekly walking data for 15 weeks in 2020, via the smartphone healthcare app, which is managed by 25 public health offices of the Seoul government. Results: According to the findings, the level of prevalence of regular walking (PRW) has a significant difference before and after the outbreak, and every interval of the four-stage COVID-19 phases, that is, pre-pandemic, initiation, acceleration, and deceleration. The level of PRW sharply decreased during initiation and acceleration intervals. In the deceleration interval of COVID-19, the PRW kept increasing, but it has not yet reached the same level as the previous year when the COVID-19 did not exist. Conclusions: As a preliminary study, this study explains empirically how COVID-19 changed PRW in Seoul. It would be helpful to enhance our understanding of the changes in physical inactivity in the pandemic period.

scholarly journals Plasma Exosomal miRNA Levels after Radiotherapy Are Associated with Early Progression and Metastasis of Cervical Cancer: A Pilot Study

2021 ◽  
Vol 10 (10) ◽  
pp. 2110
Author(s):  
Oyeon Cho ◽  
Do-Wan Kim ◽  
Jae-Youn Cheong
Keyword(s):  
Cervical Cancer ◽  
Pilot Study ◽  
Biological Functions ◽  
Patients With Cancer ◽  
Initial Stage ◽  
Exosomal Mirnas ◽  
Early Progression ◽  
Before And After ◽  
Microenvironmental Factors ◽  
Exosomal Mirna

Plasma exosomal miRNAs are key regulators of cell-cell interactions associated with several biological functions in patients with cancer. This pilot study aimed to investigate the log2 fold change (log2FC) of the expression of exosomal miRNAs and related mRNAs in the blood of patients with cervical cancer to identify prognostic markers better than those currently available. We sequenced plasma exosomal RNA from 56 blood samples collected from 28 patients with cervical cancer, who had been treated with concurrent chemoradiotherapy (CCRT). Changes in the expression of miRNAs and mRNAs before and after CCRT were represented as log2FC. Their biological functions were studied by miRNA-mRNA network analysis, using ingenuity pathway analysis, after the selection of two groups of miRNAs, each associated with early progression (EP) and metastasis, also described as initial stage. Seven patients experienced EP, three of whom died within four months after progression. Reduced levels of miR-1228-5p, miR-33a-5p, miR-3200-3p, and miR-6815-5p and increased levels of miR-146a-3p in patients with EP revealed unresolved inflammation, with accompanying increased expression of PCK1 and decreased expression of FCGR1A. Increased levels of miR-605-5p, miR-6791-5p, miR-6780a-5p, and miR-6826-5p and decreased levels of miR-16-1-3p (or 15a-3p) were associated with the degree of metastasis and led to the systemic activation of myeloid, endothelial, and epithelial cells, as well as neurons, phagocytes, and platelets. Log2FCs in the expression of miRNAs and mRNAs from plasma exosomes after CCRT are associated with EP and metastasis, reflecting unresolved inflammation and systemic microenvironmental factors, respectively. However, this study, supported by preliminary data insufficient to reach clear conclusions, should be verified in larger prospective cohorts.

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