scholarly journals Gut microbiome composition in the Hispanic Community Health Study/Study of Latinos is shaped by geographic relocation, environmental factors, and obesity

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Robert C. Kaplan ◽  
Zheng Wang ◽  
Mykhaylo Usyk ◽  
Daniela Sotres-Alvarez ◽  
Martha L. Daviglus ◽  
...  
Keyword(s):  
Community Health ◽  
Gut Microbiome ◽  
Alpha Diversity ◽  
Amplicon Sequencing ◽  
Shannon Index ◽  
Health Study ◽  
Rrna Gene ◽  
Cross Sectional ◽  
Hispanic Community ◽  
The Usa

Abstract Background Hispanics living in the USA may have unrecognized potential birthplace and lifestyle influences on the gut microbiome. We report a cross-sectional analysis of 1674 participants from four centers of the Hispanic Community Health Study/Study of Latinos (HCHS/SOL), aged 18 to 74 years old at recruitment. Results Amplicon sequencing of 16S rRNA gene V4 and fungal ITS1 fragments from self-collected stool samples indicate that the host microbiome is determined by sociodemographic and migration-related variables. Those who relocate from Latin America to the USA at an early age have reductions in Prevotella to Bacteroides ratios that persist across the life course. Shannon index of alpha diversity in fungi and bacteria is low in those who relocate to the USA in early life. In contrast, those who relocate to the USA during adulthood, over 45 years old, have high bacterial and fungal diversity and high Prevotella to Bacteroides ratios, compared to USA-born and childhood arrivals. Low bacterial diversity is associated in turn with obesity. Contrasting with prior studies, our study of the Latino population shows increasing Prevotella to Bacteroides ratio with greater obesity. Taxa within Acidaminococcus, Megasphaera, Ruminococcaceae, Coriobacteriaceae, Clostridiales, Christensenellaceae, YS2 (Cyanobacteria), and Victivallaceae are significantly associated with both obesity and earlier exposure to the USA, while Oscillospira and Anaerotruncus show paradoxical associations with both obesity and late-life introduction to the USA. Conclusions Our analysis of the gut microbiome of Latinos demonstrates unique features that might be responsible for health disparities affecting Hispanics living in the USA.

Abstract P277: Lower Gut Microbial Diversity in Non-alcoholic Fatty Liver Disease: Results From the Hispanic Community Health Study/Study of Latinos (HCHS/SOL)

Circulation ◽  
2020 ◽  
Vol 141 (Suppl_1) ◽  
Author(s):  
Jessica S Williams-Nguyen ◽  
Mark H Kuniholm ◽  
Zheng Wang ◽  
Jin Choul Chai ◽  
Jee-Young Moon ◽  
...  
Keyword(s):  
Liver Disease ◽  
Gut Microbiome ◽  
Beta Diversity ◽  
Fatty Liver Disease ◽  
Alpha Diversity ◽  
Abundant Species ◽  
Shannon Index ◽  
Health Study ◽  
Hispanic Community ◽  
Latino Adults

Introduction: Nonalcoholic fatty liver disease (NAFLD) is associated with obesity and a leading cause of chronic liver disease in the United States (US). Prevalence of both conditions is higher in US Hispanics/Latinos compared to non-Hispanic whites. Composition of the gut microbiome, comprising the microorganisms in the gastrointestinal tract, has been associated with both NAFLD and obesity in animal and human studies, but there is currently no consensus on which microbial changes are associated with these two conditions. Data on the relation of the gut microbiome with NAFLD in Hispanic/Latino populations are limited. Hypothesis: We hypothesized that gut bacterial diversity is associated with NAFLD in Hispanic/Latino adults. Methods: This analysis included 2587 Hispanic/Latino adults in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) with fecal samples. The gut microbiome was characterized using shotgun metagenomic sequencing and taxonomic classification by the SHOGUN pipeline with the RefSeq 82 prokaryotic genome database. NAFLD was defined based on gender-specific liver function test thresholds developed in the National Health and Nutrition Examination Survey (NHANES) after excluding participants with other known causes of liver dysfunction. Alpha and beta diversity were compared between groups defined by NAFLD and obesity using multinomial logistic regression and PERMANOVA, respectively. The difference in relative abundance for the 15 most abundant species were compared using the Wilcoxon rank sum test. Results: There were 395 (15%) NAFLD cases among 2587 participants, and 1718 (66%) were women. Prevalence of obesity (BMI ≥ 30) was 58% and 42% among those with and without NAFLD, respectively. Higher bacterial alpha diversity (Shannon index) was associated with lower odds of NAFLD with (OR = 0.58, p = 0.0005) or without obesity (OR = 0.70, p = 0.04) compared to participants with neither condition after adjusting for age, gender, Hispanic background, diabetes, dyslipidemia and hypertension. In the same model, higher Shannon index was also associated with lower odds of obesity in the absence of NALFD (OR = 0.78, p = 0.01). Beta diversity (Bray-Curtis) did not differ significantly by NAFLD/obesity groups (p = 0.78) or by NAFLD alone (p = 0.30). Among the 15 most abundant species across samples, 4 ( Bacteroides uniformis , Odoribacter splanchnicus , Oscillibacter sp. ER4, and Alistipes shahii ) had significantly lower abundance in those with NAFLD compared to those without NAFLD irrespective of obesity. Conclusion: This study reveals that bacterial alpha diversity but not beta diversity is independently associated with the related conditions, NAFLD and obesity, in US Hispanic/Latino adults. Future work will explore associations between NAFLD and functional capacity of gut microbial components as well as considering relevant host genetic variants in this population.

scholarly journals Cross-sectional and prospective associations between sleep regularity and metabolic health in the Hispanic Community Health Study/Study of Latinos

SLEEP ◽  
2020 ◽  
Author(s):  
Josef Fritz ◽  
Andrew J K Phillips ◽  
Larissa C Hunt ◽  
Akram Imam ◽  
Kathryn J Reid ◽  
...  
Keyword(s):  
Community Health ◽  
Cell Function ◽  
Diabetes Risk ◽  
Health Study ◽  
Diabetes Incidence ◽  
Antidiabetic Medication ◽  
Cross Sectional ◽  
Hispanic Community ◽  
Changes Over Time ◽  
Over Time

Abstract Study Objectives Sleep is an emergent, multi-dimensional risk factor for diabetes. Sleep duration, timing, quality, and insomnia have been associated with diabetes risk and glycemic biomarkers, but the role of sleep regularity in the development of metabolic disorders is less clear. Methods We analyzed data from 2107 adults, aged 19–64 years, from the Sueño ancillary study of the Hispanic Community Health Study/Study of Latinos, followed over a mean of 5.7 years. Multivariable-adjusted complex survey regression methods were used to model cross-sectional and prospective associations between the sleep regularity index (SRI) in quartiles (Q1-least regular, Q4-most regular) and diabetes (either laboratory-confirmed or self-reported antidiabetic medication use), baseline levels of insulin resistance (HOMA-IR), beta-cell function (HOMA-β), hemoglobin A1c (HbA1c), and their changes over time. Results Cross-sectionally, lower SRI was associated with higher odds of diabetes (odds ratio [OR]Q1 vs. Q4 = 1.64, 95% CI: 0.98–2.74, ORQ2 vs. Q4 = 1.12, 95% CI: 0.70–1.81, ORQ3 vs. Q4 = 1.00, 95% CI: 0.62–1.62, ptrend = 0.023). The SRI effect was more pronounced in older (aged ≥ 45 years) adults (ORQ1 vs. Q4 = 1.88, 95% CI: 1.14–3.12, pinteraction = 0.060) compared to younger ones. No statistically significant associations were found between SRI and diabetes incidence, as well as baseline HOMA-IR, HOMA-β, and HbA1c values, or their changes over time among adults not taking antidiabetic medication. Conclusions Our results suggest that sleep regularity represents another sleep dimension relevant for diabetes risk. Further research is needed to elucidate the relative contribution of sleep regularity to metabolic dysregulation and pathophysiology.

Alterations of gut microbiome in autoimmune hepatitis

Gut ◽  
2019 ◽  
Vol 69 (3) ◽  
pp. 569-577 ◽  
Author(s):  
Yiran Wei ◽  
Yanmei Li ◽  
Li Yan ◽  
Chunyan Sun ◽  
Qi Miao ◽  
...  
Keyword(s):  
Autoimmune Hepatitis ◽  
Gut Microbiome ◽  
Alpha Diversity ◽  
Disease Status ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Healthy Controls ◽  
Microbial Composition ◽  
Cross Sectional ◽  
Treatment Naïve

ObjectiveThe significance of the liver-microbiome axis has been increasingly recognised as a major modulator of autoimmunity. The aim of this study was to take advantage of a large well-defined corticosteroids treatment-naïve group of patients with autoimmune hepatitis (AIH) to rigorously characterise gut dysbiosis compared with healthy controls.DesignWe performed a cross-sectional study of individuals with AIH (n=91) and matched healthy controls (n=98) by 16S rRNA gene sequencing. An independent cohort of 28 patients and 34 controls was analysed to validate the results. All the patients were collected before corticosteroids therapy.ResultsThe gut microbiome of steroid treatment-naïve AIH was characterised with lower alpha-diversity (Shannon and observed operational taxonomic units, both p<0.01) and distinct overall microbial composition compared with healthy controls (p=0.002). Depletion of obligate anaerobes and expansion of potential pathobionts including Veillonella were associated with disease status. Of note, Veillonella dispar, the most strongly disease-associated taxa (p=8.85E–8), positively correlated with serum level of aspartate aminotransferase and liver inflammation. Furthermore, the combination of four patients with AIH-associated genera distinguished AIH from controls with an area under curves of approximately 0.8 in both exploration and validation cohorts. In addition, multiple predicted functional modules were altered in the AIH gut microbiome, including lipopolysaccharide biosynthesis as well as metabolism of amino acids that can be processed by bacteria to produce immunomodulatory metabolites.ConclusionOur study establishes compositional and functional alterations of gut microbiome in AIH and suggests the potential for using gut microbiota as non-invasive biomarkers to assess disease activity.

scholarly journals 83 Lifelong dynamics of the swine gut microbiome: from birth to market

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 48-48
Author(s):  
Xiaofan Wang ◽  
Tsung Cheng Tsai ◽  
Charles V Maxwell ◽  
Jiangchao Zhao
Keyword(s):  
Growth Performance ◽  
Gut Microbiome ◽  
Community Dynamics ◽  
Alpha Diversity ◽  
Illumina Miseq ◽  
Shannon Index ◽  
Growth Stages ◽  
Rrna Gene ◽  
16 S Rrna

Abstract Despite the recent advances in the swine gut microbiomes during different growth stages, a comprehensive longitudinal study of the lifelong dynamics of the swine gut microbiome is lacking. To fill this gap of knowledge, we selected seventeen piglets (PIC29*380) that were born on the same date from three sows. We collected a total of 274 rectal swabs during lactation (d 0, 11, 20), nursery (d 27, 33, 41, 50, 61), growing (d 76, 90, 104, 116), and finishing (d 130, 146, 159, and 174) stages. Samples were extracted using the Powersoil DNA isolation kit (Qiagen, Hilden, Germany) and sequenced with an Illumina Miseq sequencer targeting the V4 region of the 16 S rRNA gene. Sequences were analyzed with the Deblur algorithm in the QIIME2 package. In general, alpha diversity including community richness (e.g., number of observed features, Chao1) and diversity (e.g., Shannon Index) showed an overall trend of increasing from lactation to the finishing stage (P < 0.01). Gradual and significant changes in community structures were also observed along the four growth stages (ANOSIM, R = 0.66; P < 0.01). Non-parametric permutational multivariate analysis of variance shows that main factors driving the lifelong community dynamics included age and diet. Seventeen phylum members were discovered in the lifelong pig gut microbiome with Firmicutes and Bacteroidetes being the most abundant phyla. LEfSe analysis revealed 63 bacterial features that are stage specific. By using a regressing tree based Random Forest model we identified five bacterial features that are associated with swine growth performance including features 26 (Turicibacteraceae Turicibacter), 27 (Clostridium butyricum), 18 (Clostridiaceae), 19 (Clostridium perfringens) and 4 (Clostridiaceae). Characterization of the lifelong dynamics of 17 healthy pigs from birth to market provides a foundation for gut microbiome studies focusing on swine development, health and growth performance.

scholarly journals Variations of dietary intake by glycemic status and Hispanic/Latino heritage in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL)

2018 ◽  
Vol 6 (1) ◽  
pp. e000486 ◽  
Author(s):  
Sarah S Casagrande ◽  
Daniela Sotres-Alvarez ◽  
Larissa Avilés-Santa ◽  
Matthew J O’Brien ◽  
Cristina Palacios ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Community Health ◽  
Energy Intake ◽  
Sodium Intake ◽  
Health Study ◽  
Fiber Intake ◽  
Glycemic Status ◽  
Cross Sectional ◽  
Hispanic Community ◽  
Diagnosed Diabetes

ObjectiveA healthy diet is important for diabetes prevention and control; however, few studies have assessed dietary intake among US Hispanics/Latinos, a diverse population with a significant burden of diabetes. To address this gap in the literature, we determined intake of energy, macro/micronutrients, and vitamin supplements among Hispanics/Latinos by glycemic status and heritage.Research design and methodsCross-sectional study of adults aged 18–74 years from the Hispanic Community Health Study/Study of Latinos (2008–2011) with complete baseline data on glycemic status and two 24-hour dietary recalls (n=13 089). Age-adjusted and sex-adjusted and multivariable-adjusted measures of intake were determined by glycemic status and heritage.ResultsMean age-adjusted and sex-adjusted energy intake was significantly lower among Hispanics/Latinos with diagnosed diabetes compared with those with normal glycemic status (1665 vs 1873 kcal, P<0.001). Fiber intake was higher among those with diagnosed diabetes versus normal glycemic status (P<0.01). Among those with diagnosed diabetes, energy intake was highest among those with Cuban heritage compared with most other heritage groups (P<0.01 for all, except Mexicans), but there was no difference after additional adjustment. Fiber intake was significantly lower for those of Cuban heritage (vs Dominican, Central American, and Mexican), and sodium intake was significantly higher (vs all other heritage groups) (P<0.01 for all); findings were null after additional adjustment. There was no difference in supplemental intake of vitamin D, calcium, magnesium, or potassium by glycemic status.ConclusionsAs part of the care of Hispanics/Latinos with diabetes, attention should be made to fiber and sodium consumption.

Exploring the Association of Self-Reported Physical Activity with Peripheral Arterial Disease Severity: A Cross-Sectional Study from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) Database

2021 ◽  
Author(s):  
Monira Ibrahim Aldhahi ◽  
Mohammed M. Alshehri ◽  
Abdulfattah Saeed Alqahtani
Keyword(s):  
Physical Activity ◽  
Community Health ◽  
Sex Education ◽  
Ankle Brachial Index ◽  
Health Study ◽  
Cross Sectional ◽  
Physically Active ◽  
Metabolic Equivalents ◽  
Hispanic Community ◽  
Physically Inactive

Abstract Background Engaging in physical activity (PA) has been proved to reduce the risk of developing cardiovascular diseases. In patients with peripheral artery disease (PAD), diminished PA predicts high overall mortality. However, it is unknown to what extent participation in PA is associated with PAD severity. Therefore, the overarching aim of this study was to investigate the association between PAD severity and PA levels using the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) database. Methods This was a cross-sectional cohort study that included 495 participants with PAD and a total of 14,536 participants without PAD from the HCHS/SOL database. The Global Physical Activity Questionnaire was administered to assess the time spent weekly in performing moderate to vigorous PA (MVPA) during work, leisure time, and transportation. The Ankle–Brachial Index (ABI) was used to measure PAD. PA status was categorized on the basis of MVPA as follows: physically active (MVPA ≥ 150 min) and physically inactive (combined MVPA < 150 min). Eventually, the total sample was then classified as follows: normal ABI and physically active, normal ABI but physically inactive, PAD and physically active, and PAD but physically inactive. Regression models were used to investigate the association between different types of PA and the severity of PAD. Results Of the participants, 235 (47%) were involved in no to insufficient PA, and 260 (53%) engaged in at least 150 min/week of MVPA. The majority of participants (54%) with mild PAD were physically active, and 58% of those with severe PAD were physically inactive. After adjustment for covariates (age, sex, education, smoking status, body mass index, and statins), inactive people with a diagnosis of PAD were more likely to have severe PAD (β = .79, p = .02). In addition, decreases in work-related PA, total metabolic equivalents, and total PA were associated with increased PAD severity. Conclusions It was found that MVPA patterns were associated with PAD severity, and they explained high severity among patients with PAD who were physically inactive. These findings highlight the necessity of interventions in increasing PA in these participants. Future studies are required to identify appropriate exercise regimens or home-based programs to help patients with severe PAD meet the current PA recommendations.

scholarly journals Plasma Metabolites Related to Peripheral and Hepatic Insulin Sensitivity Are Not Directly Linked to Gut Microbiota Composition

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2308
Author(s):  
Annefleur M. Koopen ◽  
Nicolien C. de Clercq ◽  
Moritz V. Warmbrunn ◽  
Hilde Herrema ◽  
Mark Davids ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Gut Microbiota ◽  
Prediction Models ◽  
Alpha Diversity ◽  
Amplicon Sequencing ◽  
Plasma Metabolites ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Cross Sectional ◽  
Gut Microbiota Composition

Plasma metabolites affect a range of metabolic functions in humans, including insulin sensitivity (IS). A subset of these plasma metabolites is modified by the gut microbiota. To identify potential microbial–metabolite pathways involved in IS, we investigated the link between plasma metabolites, gut microbiota composition, and IS, using the gold-standard for peripheral and hepatic IS measurement in a group of participants with metabolic syndrome (MetSyn). In a cross-sectional study with 115 MetSyn participants, fasting plasma samples were collected for untargeted metabolomics analysis and fecal samples for 16S rRNA gene amplicon sequencing. A two-step hyperinsulinemic euglycemic clamp was performed to assess peripheral and hepatic IS. Collected data were integrated and potential interdependence between metabolites, gut microbiota, and IS was analyzed using machine learning prediction models. Plasma metabolites explained 13.2% and 16.7% of variance in peripheral and hepatic IS, respectively. Fecal microbiota composition explained 4.2% of variance in peripheral IS and was not related to hepatic IS. Although metabolites could partially explain the variances in IS, the top metabolites related to peripheral and hepatic IS did not significantly correlate with gut microbiota composition (both on taxonomical level and alpha-diversity). However, all plasma metabolites could explain 18.5% of the variance in microbial alpha-diversity (Shannon); the top 20 metabolites could even explain 44.5% of gut microbial alpha-diversity. In conclusion, plasma metabolites could partially explain the variance in peripheral and hepatic IS; however, these metabolites were not directly linked to the gut microbiota composition, underscoring the intricate relation between plasma metabolites, the gut microbiota, and IS in MetSyn

Sex-Specific Prevalence, Demographic Characteristics, and Risk Factors of Tinnitus in the Hispanic Community Health Study/Study of Latinos

2022 ◽  
pp. 1-13
Author(s):  
Michelle L. Arnold ◽  
Sumitrajit Dhar ◽  
David J. Lee ◽  
Krista M. Perreira ◽  
Daniel Pupo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Community Health ◽  
Noise Exposure ◽  
Survey Methodology ◽  
Smoking History ◽  
Chronic Tinnitus ◽  
Health Study ◽  
Lifestyle Modifications ◽  
Cross Sectional ◽  
Hispanic Community

Purpose: The aim of this study was to determine the prevalence of any and chronic tinnitus among female and male individuals from varied Hispanic/Latino backgrounds and to estimate associations between risk factors for chronic tinnitus. Method: Our analysis used cross-sectional baseline data collected from 2008 to 2011 from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL). Prevalence estimates and multivariable logistic regression were conducted using survey methodology. Participants included 15,768 adults (8,229 women and 7,539 men) aged 18–76 years. The primary outcome of interest was chronic tinnitus, defined as self-reported tinnitus lasting ≥ 5 min at a time and at least once per week. We hypothesized that after adjusting for covariates, the risk factors of depressed and anxious symptoms, smoking history, hypertension, and noise exposure history would be associated with higher odds of chronic tinnitus. Results: Unstratified prevalence for any tinnitus was 32.9%, and for chronic tinnitus, it was 12.1%. Sex-stratified results demonstrated that 2,995 female individuals (36.4%) and 2,187 male individuals (29.0%) reported any tinnitus, and of these, 1,043 female individuals (12.7%) and 870 male individuals (11.5%) reported chronic tinnitus. In the fully adjusted model, depressed and anxious symptoms as well as recreational noise exposure were associated with higher odds of chronic tinnitus in female individuals (odds ratios [ ORs ] = 1.06, confidence interval [CI; 1.04, 1.07]; 1.02, CI [1.01, 1.04]; and 1.40, CI [1.20, 1.62]) and in male individuals ( OR s = 1.06, CI [1.03, 1.08]; 1.05, CI [1.02, 1.08]; and 1.30, CI [1.05, 1.65]). Current smoking was a risk factor for chronic tinnitus in male individuals ( OR = 1.53, CI [1.16, 2.02]). Conclusions: Prevalence of any and chronic tinnitus in the HCHS/SOL baseline cohort is higher than that reported in previous studies, particularly among female individuals. Understanding risk factors associated with tinnitus is important for the development of culturally and linguistically appropriate public health programs that consider sex differences and promote lifestyle modifications known to lower the odds of experiencing tinnitus.

Abstract P230: Association of Accelerometer-Measured Physical Activity and Cardiovascular Health in the Hispanic Community Health Study/Study of Latinos

Circulation ◽  
2020 ◽  
Vol 141 (Suppl_1) ◽  
Author(s):  
Priscilla Vasquez ◽  
Ramon A Durazo-Arvizu ◽  
David Marquez ◽  
Maria Argos ◽  
Melissa Lamar ◽  
...  
Keyword(s):  
Physical Activity ◽  
Blood Pressure ◽  
Blood Glucose ◽  
Community Health ◽  
Vigorous Physical Activity ◽  
Health Indicators ◽  
Health Study ◽  
Cross Sectional ◽  
Hispanic Community ◽  
Latino Adults

Background: Detailed information on associations between accelerometer-measured moderate-vigorous physical activity (MVPA) and cardiovascular (CV) health among Hispanic/Latino adults are unavailable. Methods: Cross-sectional data from 12,008 Hispanic/Latino adults aged 18-74 years in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) 2008-2011 were analyzed using complex survey design methods. Poisson regression models were used to examine the associations between accelerometer-measured MVPA and CV health indicators (blood glucose, total cholesterol, blood pressure, body mass index [BMI], and smoking) and a composite of low CV risk. Results: Compared to high MVPA, low MVPA was associated with a higher prevalence of diabetes, hypercholesterolemia, obesity, smoking, but not hypertension. Low MVPA was associated with lower prevalence of ideal blood glucose, blood pressure, BMI and low CV risk. Similar results were observed with non-adherence of PA guideline recommendations. Conclusions: Overall in Hispanic/Latino adults, high accelerometer-measured MVPA and meeting PA guideline recommendations were associated with favorable CV health.

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