scholarly journals Prospective serological and molecular cross-sectional study focusing on Bartonella and other blood-borne organisms in cats from Catalonia (Spain)

2022 ◽  
Vol 15 (1) ◽  
Author(s):  
Alejandra Álvarez-Fernández ◽  
Ricardo Maggi ◽  
Gerard Eduard Martín-Valls ◽  
Marta Baxarias ◽  
Edward Bealmear Breitschwerdt ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bartonella Henselae ◽  
Cross Sectional Study ◽  
Molecular Techniques ◽  
Cross Sectional ◽  
Bartonella Quintana ◽  
Antibody Assays ◽  
Serological Studies ◽  
Blood Specimens ◽  
Associated Risk Factors

Abstract Background There is limited clinical or epidemiological knowledge regarding Bartonella infection in cats, and no serological studies have compared the presence of antibodies against different Bartonella species. Moreover, there are limited feline Bartonella studies investigating co-infections with other vector-borne pathogens and the associated risk factors. Therefore, the objective of this study was to investigate Bartonella spp. infections and co-infections with other pathogens in cats from Barcelona (Spain) based on serological and/or molecular techniques and to determine associated risk factors. Methods We studied colony and owned cats (n = 135). Sera were tested for Bartonella henselae-, Bartonella quintana-, and Bartonella koehlerae-specific antibodies using endpoint in-house immunofluorescence antibody assays. Bartonella real-time PCR (qPCR) and conventional PCR (cPCR) were performed. In addition, cPCR followed by DNA sequencing was performed for other pathogenic organisms (Anaplasma, Babesia, Cytauxzoon, Ehrlichia, Hepatozoon, hemotropic Mycoplasma, and Theileria spp.). Results From 135 cats studied, 80.7% were seroreactive against at least one Bartonella species. Bartonella quintana, B. koehlerae, and B. henselae seroreactivity was 67.4, 77.0, and 80.7%, respectively. Substantial to almost perfect serological agreement was found between the three Bartonella species. Colony cats were more likely to be Bartonella spp.-seroreactive than owned cats. Moreover, cats aged ≤ 2 years were more likely to be Bartonella spp.-seroreactive. Bartonella spp. DNA was detected in the blood of 11.9% (n = 16) of cats. Cats were infected with B. henselae (n = 12), B. clarridgeiae (n = 3), and B. koehlerae (n = 1). Mycoplasma spp. DNA was amplified from 14% (n = 19) of cat blood specimens. Cats were infected with Mycoplasma haemofelis (n = 8), Candidatus M. haemominutum (n = 6), Candidatus Mycoplasma turicensis (n = 4), and Mycoplasma wenyonii (n = 1). Anaplasma, Babesia, Cytauxzoon, Ehrlichia spp., Hepatozoon, and Theileria spp. DNA was not amplified from any blood sample. Of the 16 Bartonella spp.-infected cats based on PCR results, six (37%) were co-infected with Mycoplasma spp. Conclusions Bartonella spp. and hemoplasma infections are prevalent in cats from the Barcelona area, whereas infection with Anaplasma spp., Babesia, Cytauxzoon, Ehrlichia spp., Hepatozoon, and Theileria infections were not detected. Co-infection with hemotropic Mycoplasma appears to be common in Bartonella-infected cats. To our knowledge, this study is the first to document M. wenyonii is infection in cats. Graphical Abstract

scholarly journals Magnitude of spontaneous preterm birth and its associated factors among preterm birth in NICU wards in Asella Teaching and Referral Hospital, Asella, Oromia, Ethiopia

2021 ◽  
Vol 49 (8) ◽  
pp. 030006052110346
Author(s):  
Techane Sisay Tuji ◽  
Addisu Dabi Wake ◽  
Gezahegn Badeg Adere ◽  
Aselefu Beka Wedajo ◽  
Batu Dekeba Obole ◽  
...  
Keyword(s):  
Risk Factors ◽  
Preterm Birth ◽  
Healthcare Providers ◽  
Preterm Births ◽  
Cross Sectional Study ◽  
Spontaneous Preterm Birth ◽  
Related Factors ◽  
Cross Sectional ◽  
Bivariate Logistic Regression ◽  
Associated Risk Factors

Objective To assess the prevalence of spontaneous preterm births and to identify the associated risk factors. Methods This single-centre cross-sectional study enrolled women that experienced a preterm birth as registered on the neonatal log-book between 30 December 2019 and 30 December 2020. A pre-tested structured checklist was used to collect data (sociodemographic characteristics; obstetric-related factors; medical history; and pregnancy-related factors). Bivariate logistic regression analyses were applied to identify factors associated with spontaneous preterm birth. A multivariate model identified significant independent risk factors. Results A total of 310 patients participated in the study. The prevalence of spontaneous preterm birth in this population was 67.1% (208 of 310; 95% confidence interval [CI] 61.5, 71.9). Patients without a partner (adjusted odds ratio [AOR] = 1.470, 95% CI 1.23, 4.42), patients residing in a rural area (AOR = 2.51, 95% CI 1.123, 5.513) and those with a history of PIH during their current pregnancy (AOR = 0.104, 95% CI 0.053, 0.014) were significantly more likely to have a spontaneous preterm birth. Conclusion The prevalence of spontaneous preterm birth in in this study was high. Healthcare providers and all stakeholders should focus on screening pregnant women at the risk of spontaneous preterm birth.

scholarly journals Prevalence of dysmenorrhoea, associated risk factors and its relationship with academic performance among graduating female university students in Ethiopia: a cross-sectional study

BMJ Open ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. e043814
Author(s):  
Mesfin Tadese ◽  
Andargachew Kassa ◽  
Abebaw Abeje Muluneh ◽  
Girma Altaye
Keyword(s):  
Risk Factors ◽  
Academic Performance ◽  
University Students ◽  
Cross Sectional Study ◽  
Secondary Outcome ◽  
Sectional Study ◽  
Cross Sectional ◽  
Grade Point ◽  
Significant Difference ◽  
Associated Risk Factors

ObjectivesThe study aimed to provide an association between dysmenorrhoea and academic performance among university students in Ethiopia. Further, the study attempts to determine the prevalence and associated risk factors of dysmenorrhoea.Design and methodInstitution-based cross-sectional study was conducted from 1 April to 28 April 2019. A semistructured and pretested self-administered questionnaire was used to collect data. Binary logistic regression analysis and one-way analysis of variance were performed to model dysmenorrhoea and academic performance, respectively.Setting and participantsEthiopia (2019: n=647 female university students).OutcomesThe primary outcome is dysmenorrhoea, which has been defined as painful menses that prevents normal activity and requires medication. The self-reported cumulative grade point average of students was used as a proxy measure of academic performance, which is the secondary outcome.ResultsThe prevalence of dysmenorrhoea was 317 (51.5%). The educational status of father (adjusted OR (AOR) (95% CI) 2.64 (1.04 to 6.66)), chocolate consumption (AOR (95% CI) 3.39 (95% 1.28 to 8.93)), daily breakfast intake (<5 days/week) (AOR (95% CI) 0.63 (0.42 to 0.95)), irregular menstrual cycle AOR (95% CI) 2.34 (1.55 to 3.54)) and positive family history of dysmenorrhoea AOR (95% CI) 3.29 (2.25 to 4.81)) had statistically significant association with dysmenorrhoea. There was no statistically significant difference in academic performance among students with and without dysmenorrhoea (F (3611)=1.276, p=0.28)).ConclusionsDysmenorrhoea was a common health problem among graduating University students. However, it has no statistically significant impact on academic performance. Reproductive health officers should educate and undermine the negative academic consequences of dysmenorrhoea to reduce the physical and psychological stress that happens to females and their families.

scholarly journals Occurrence, associated risk factors, and treatment of surgical site infections in Pakistan

2020 ◽  
Vol 18 ◽  
pp. 205873922096054
Author(s):  
Faiz Ullah Khan ◽  
Yu Fang ◽  
Zakir Khan ◽  
Farman Ullah Khan ◽  
Zafar Iqbal Malik ◽  
...  
Keyword(s):  
Risk Factors ◽  
Transurethral Resection ◽  
Surgical Site Infections ◽  
Exploratory Laparotomy ◽  
Cross Sectional Study ◽  
Surgical Patients ◽  
Related Factors ◽  
Cross Sectional ◽  
Mortality And Morbidity ◽  
Associated Risk Factors

Globally, surgical site infections are one of the common infections which lead to a large amount of mortality and morbidity in postsurgical care. The risk for surgical site infection is multidimensional which includes mainly; patient, surgery, and hospital-related factors. This study is aimed to determine the burden of SSIs along with contributed risk factors. A prospective observational cross-sectional study was conducted in one of the largest public-sector hospitals in Pakistan. A total of 412 patients were recruited in the study with full consent and monitored for 30 days after surgery with direct and indirect surveillance. Overall, in seven different surgical procedures the incidence (29.8%) rate of SSI was observed; in appendectomy ( n = 17, 4.1%), exploratory laparotomy ( n = 51, 12.6%), laparoscopic cholecystectomy ( n = 12, 2.90%), mesh repair ( n = 17, 4.01%), thyroidectomy (5, 1.2%), transurethral resection of the prostate ( n = 11, 2.6%), and transurethral resection of the bladder (10, 2.4%). The average SSI rate in every single procedure was about 18 (4.27%) per surgical procedure out of 123 (29.85%) SSI cases. Types of SSI identified were superficial, deep incisional and organ/space ( n = 76, 18.4%, n = 23, 5.5%, and n = 24, 5.7%). Incidence of SSIs during admission, at readmission, and post-surveillance cases were ( n = 50, 12.1%, n = 25, 6.0% and n = 48, 11.6%). Associated risk factors found contributed to the incidence of SSI ( p < 0.05). Pre-operative ( n = 348, 84.5%) and 6 (1.5%) surgical patients did not received the post-operative antibiotics. The P. aeruginosa ( n = 15, 12.1%) and S. aureus (13, 10.5%). Cefoperazone and sulbactam were the most prescribed antibiotics. Associated risk factors and treatment outcomes of surgical patients have a direct association with the incidence of SSI. Hospital-based antimicrobial stewardship, implementation of surgical guidelines, patient care, and education are needed to develop at wards level in hospitals.

scholarly journals Seroprevalence of AsymptomaticLeishmania donovaniamong Laborers and Associated Risk Factors in Agricultural Camps of West Armachiho District, Northwest Ethiopia: A Cross-Sectional Study

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Animen Ayehu ◽  
Yibeltal Aschale ◽  
Wossenseged Lemma ◽  
Animut Alebel ◽  
Ligabaw Worku ◽  
...  
Keyword(s):  
Risk Factors ◽  
Leishmania Donovani ◽  
Public Health Problem ◽  
Cross Sectional Study ◽  
Northwest Ethiopia ◽  
Control Measures ◽  
Sectional Study ◽  
Cross Sectional ◽  
Associated Risk Factors ◽  
Study Participants

Background. Visceral leishmaniasis (VL, also called kala-azar) is a public health problem in Ethiopia, especially in sesame and sorghum growing areas. Compared to other populations, labor migrants are the most exposed. Knowing the seroprevalence ofLeishmania donovaniand associated risk factors is essential to design appropriate control measures. The main aim of this study was to assess the seroprevalence of asymptomaticL. donovaniamong laborers and associated risk factors in agricultural camps of West Armachiho district, Northwest Ethiopia. Therefore, this study was conducted to know the seroprevalence and associated risk factors ofL. donovaniinfection.Method. A cross-sectional study was conducted among 185 laborers from October to December 2017. A simple random sampling technique was used to select study participants from selected agricultural camps. After obtaining written informed consent, data were collected using a structured pretested Amharic version questionnaire using the interview technique. A single finger prick blood sample was collected from the study participants and the blood samples were subjected to the serological diagnostic method using the rk39 kit. The multivariable logistic regression model was used to identify risk factors associated withL. donovaniinfection.Result. Among 185 participants examined using rk39, 14 (7.6%) were seroreactive forL. donovani.Leishmania donovaniinfection had a statistically significant association with sleeping underBalanitestrees (AOR: 4.36, 95%CI: 1.186-16.06), presence of domestic animals near sleeping place (AOR: 4.68, 95% CI: 1.25-17.56), and lack of knowledge about VL transmission (AOR: 3.79, 95% CI: 1.07-13.47).Conclusion. Seroprevalence of asymptomaticL. donovaniamong laborers in agricultural camps of West Armachiho was low. Prevention measures and health education about risk factors that expose toL. donovaniinfection for the laborers are essential to prevent the spread of the disease.

scholarly journals Prevalence of stroke and associated risk factors: a population based cross sectional study from northeast China

BMJ Open ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. e015758 ◽  
Author(s):  
Fu-Liang Zhang ◽  
Zhen-Ni Guo ◽  
Yan-Hua Wu ◽  
Hao-Yuan Liu ◽  
Yun Luo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Ischaemic Stroke ◽  
Northeast China ◽  
Haemorrhagic Stroke ◽  
Population Based ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Cerebrovascular Risk ◽  
Lack Of Exercise ◽  
Associated Risk Factors

ObjectivesEpidemiological studies aimed at stroke and its risk factors can help identify persons at higher risk and therefore promote stroke prevention strategies. We aimed to explore the current prevalence of stroke and its associated risk factors in northeast China.DesignPopulation based cross sectional study.SettingData were collected using a structured precoded questionnaire designed by the Stroke Screening and Prevention Programme of the National Health and Family Planning Commission of China, between January and March 2016.Participants4100 permanent residents, aged 40 years or older, who had lived in Dehui City of Jilin Province for more than 6 months volunteered to participate in the survey, with a response rate of 92.2%. For the purpose of the present analysis, 48 subjects were excluded due to missing values, giving a total of 4052 people included in this analysis.Main outcome measureThe questionnaire included demographic characteristics, stroke related behavioural factors, personal and family medical history of stroke, physical examination and laboratory testing.ResultsThe overall prevalence of stroke in Jilin Province was 7.2% (95% CI 6.3% to 8.2%). Of all stroke cases, 91.7% (95% CI 87.4% to 94.6%) were ischaemic stroke and 8.3% (95% CI 5.4% to 12.6%) were haemorrhagic stroke. The prevalence rates of dyslipidaemia, smoking and hypertension were ranked as the top three cerebrovascular risk factors and were 62.1%, 61.8% and 57.3%, respectively. We found that hypertension, dyslipidaemia and lack of exercise were associated with ischaemic stroke. However, only hypertension (OR=4.064, 95% CI 1.358 to 12.160) was significantly associated with haemorrhagic stroke.ConclusionsThe prevalence of stroke, especially ischaemic stroke, and associated cerebrovascular risk factors among adults aged 40 years or older in northeast China were high. A higher regional prevalence of hypertension, dyslipidaemia and lack of exercise may be responsible.

scholarly journals Diabetes Mellitus and Its Associated Risk Factors among adult Population in Bisha Province, Southwest of Saudi Arabia

2016 ◽  
Vol 9 (6) ◽  
pp. 22 ◽  
Author(s):  
Abdullah M. Al-Shahrani ◽  
Mohammed A. Al-Saleem ◽  
Mohamed O’haj ◽  
Faleh Th. Mohammed ◽  
Mutasim E. Ibrahim
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Healthy Lifestyle ◽  
Adult Population ◽  
Cross Sectional Study ◽  
World Health ◽  
Cross Sectional ◽  
Married Individuals ◽  
Health Organization ◽  
Associated Risk Factors

OBJECTIVES: To determine the prevalence and associated risk factors of diabetes mellitus (DM) among the adult population in Bisha province, Saudi Arabia.METHODS: A cross sectional study was conducted during December, 2015 using the World Health Organization STEPS wise approach for data collection. Blood glucose level and anthropometric measurements of blood pressure, height, weight, and waist circumference were performed per standard methods.  Logistic regression analysis was used to assess the prevalence and risk of diabetes.RESULTS: Of 542 adult individuals included in the study, 13.3% (72) had diabetes, of which 8.1% were previously diagnosed and 5.2% represented new cases. The proportions of DM were 14.7% for men and 10.8% for women. Diabetes was significantly higher among married compared to unmarried individuals (19.3% vs 5.5 %; p<0.001) and among individuals aged ≥40 years old compared to those <40 years (31.3% vs 9.3%; p<0.001). The risk of diabetes was significantly increased with older age (Odds Ratio=4.470; 95% CI 2.264-7.614), married individuals (OR=4.097; 95% CI 2.188-7.672), weight/obesity (OR=2.827; 95% CI 1.567-5.072), hypertension (OR=4.383; 95% CI 2.085-9.214) and having a job (OR=2.327; 95% CI 1.347-4.02). The independent risk factors predicted diabetes were hypertension (Adjusted OR=2.897; 95% CI 1.269-66.13) and job patterns (Adjusted OR=2.793; 95% CI 1.064-7.329).CONCLUSION: Different risk factors alarming diabetes among adult population in Bisha province were detected.  Strategies aimed to improving a healthy lifestyle are necessary to reduce the burden of the disease. 

scholarly journals Seroprevalence of Brucellosis and Associated Risk Factors among Indigenous Breeds of Goats in Wukari, Taraba State, Nigeria

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Olaolu T. Olufemi ◽  
Danjuma B. Dantala ◽  
Paul A. Shinggu ◽  
Umesi A. Dike ◽  
Gbeminiyi R. Otolorin ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Exact Test ◽  
The Public ◽  
Factors Associated ◽  
Males And Females ◽  
Associated Risk Factors ◽  
La Jolla

A cross-sectional study was conducted in Wukari, Taraba state, Nigeria, to determine the prevalence of Brucella antibodies and the risk factors associated with brucellosis in indigenous breeds of goats. A total of 386 goats were sampled from three political wards: Puje, Avyi, and Hospital: harvested sera samples were subjected to Rose Bengal Plate Test (RBPT). GraphPad Prism version 7.03 for Windows (GraphPad Software, La Jolla California, USA) was used to analyse the association between seroprevalence of brucellosis and age, sex, breed, location, and management system by using Chi square and Fisher’s exact test as appropriate. Brucellosis was detected in all three wards: Puje; 15%, Avyi; 6.6%, and Hospital; 7.6%. A prevalence rate of 2.8%, 8%, 18.7%, and 1% was recorded for <20-month, 22-35-month, 36-45-month, and ≥46-55-month age categories, respectively (P < 0.05). Only 9.5% was observed for male animals while 9.8% was observed for female animals with no statistical difference between the males and females. Breed-specific seroprevalence yielded 7.4%, 5.4% 12%, 12.8%, and 11.6%, for Cross, West Africa Dwarf, Red Sokoto, Kano Brown, and Sahel breeds of goat, respectively. There is an evidence of brucellosis (9.6%) in Wukari L.G.A, Taraba State, and age is a risk factor for the disease in the study area. There is a need to enlighten the public on the zoonotic potentials and economic impacts of brucellosis.

scholarly journals Sero-Prevalence of Contagious Caprine Pleuropneumonia in Goats in Afgoye District Lower Shabelle Region, Somalia

2020 ◽  
Vol 5 (2) ◽  
pp. 1-4
Author(s):  
Omar Mohamed Abdi
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Latex Agglutination ◽  
Economic Losses ◽  
High Morbidity ◽  
Cross Sectional ◽  
Mycoplasma Capricolum ◽  
Contagious Caprine Pleuropneumonia ◽  
Epidemiological Risk ◽  
Associated Risk Factors

Background: Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. Capripneumoniae (Mccp), is one of the most important goat diseases causing high morbidity and mortality, these results in heavy economic losses to countries like Somalia, which is a country affected the civil war of the 1990s. Objective: Generally, the disease presence has been reported in the country. However, there is no quantitative data on the prevalence and distribution of the disease has been scientifically reported in the country. Methods: A cross-sectional study was conducted from September 2019 to June 2020 in three villages of Afgoye district of Somalia to assess the seroprevalence and identify the associated risk factors for the occurrence of the CCPP. The risk factors considered included sex, age, and locations. Results: A total of 100 blood samples were collected and evaluated by Capri-Latex Agglutination Test (Capri-LAT). A total of 49/100 (49%, CI 95%: (38.86%-59.2%) goats were seropositive for anti-CCPP antibody. The epidemiological risk factors considered, sex, age and locations, were not significantly associated with the CCPP sero prevalence (p>0.05). However, the sero positivity was slightly higher in female (51%, 95% CI: 39.8-62.6), >3 years old goats (86.3%, 95% CI: 48.9-87.4) and Markazka village (56%, 95% CI: 41.3-70.0) compared to male (40%, 95% CI: 19.1-63.9), 1-3 years old (57.5%, 95% CI: 40.9- 73.0), and Shareeco (43.8%, 95% CI: 19.8-70.1) and Siinka (41.2%, 95% CI: 24.7-59.3) villages, respectively. Conclusion: The present study indicates the prevalence of CCPP in the studies areas and this requires practicable measures to be put in place towards the control of the disease.

scholarly journals Brucellosis in camel and human: Seroprevalence and associated risk factors in Amibara district of Afar region, Ethiopia

2021 ◽  
Author(s):  
Fekadu Gutema ◽  
Prof Kebede Amenu ◽  
Adugna Chalchisa ◽  
Prof Gezahegn Mamo
Keyword(s):  
Risk Factors ◽  
Close Contact ◽  
Mammalian Species ◽  
Raw Milk ◽  
Cross Sectional Study ◽  
Public Health Importance ◽  
Cross Sectional ◽  
North East ◽  
Afar Region ◽  
Associated Risk Factors

Abstract Background: Brucellosis is an important neglected zoonotic disease caused by infection with bacteria of the genus Brucella affecting different mammalian species including man. A cross-sectional study was conducted to estimate the seroprevalence of brucellosis in camels and human and its associated risk factors in Amibara district of Afar region, North east Ethiopia from October 2019 to May 2020Result: A total of 250 camel and 120 human sera were serially tested using the Rose Bengal Plate Test (RBPT), and Complement Fixation Test (CFT). The overall seroprevalence of camel brucellosis in the current study was 7.6% (95% CI: 4.9-11.56) using RBPT and 3.2% (95% CI: 1.63-6.2) by combined RBPT and CFT. In Human twelve (10%) of the collected sera were positive by RBPT among which only four of them (3.33%) were positive by CFT. The risk factors analysis indicated that, age, body condition, number of parity and abortion history were significantly associated with brucella seropositivity in camel(P≤0.05). In human, occupation and non-protective handling of dystocia cases showed apparent association with brucella seropositivity.Conclusion: The results of the present study indicated that, brucellosis is a common health problem in camel and human in Amibara district of Afar region. The public health importance of this disease is associated with raw milk consumption and close contact with the animals having history of recent abortion. Therefore, controlling the risk factors, establishing brucella diagnostic service in human clinics and hospitals, continuous social training with feedback assessments and overall implementing of One Health approach framework to attain optimal health for people and domestic animals in area are recommended to safeguard the health of society.

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