scholarly journals Exercise training and de-training effects on serum leptin and TNF-α in high fat induced diabetic rats

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Hamideh Dinari Ghozhdi ◽  
Ali Heidarianpour ◽  
Maryam Keshvari ◽  
Hassan Tavassoli
Keyword(s):  
Exercise Training ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Normal Diet ◽  
Aerobic Exercise Training ◽  
High Fat ◽  
Serum Leptin ◽  
Tnf Α

Abstract Background Adipocytokines, which are secreted by the adipose tissue, contribute to the pathogenesis of obesity-related complications. To evaluate this assumption, we investigated the effects of aerobic exercise training (AET), resistance exercise training (RET), and 4 weeks of de-training on serum leptin and TNF-α levels in diabetic rats. Method 36 Wistar rats were divided into normal diet (ND) (control, RET, AET) and high-fat diet (HFD) + STZ (control, RET, AET) groups. Serum insulin, leptin, and TNF-α levels were assessed by commercial ELISA kits. Also fasting blood glucose (FBG), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG) levels were measured by the colorimetric kits. Results Diabetes induction increased body weight (BW) and FBG, and decreased insulin compared to the ND rats’ groups (p < 0.001). 12-weeks of AET and RET programs in the trained diabetic rats led to a decrease in TG, LDL-C, leptin, TNF-α, and FBG, and an increase in insulin compared to the HFD + STZ-C group (p < 0.001). Besides, there was no difference between AET and RET in improving the variables studied (p > 0.05). Also, de-training led to increased BW, TG, leptin, and TNF-α compared to the end of the exercise training (p < 0.05). The correlation between the variables studied was established at different stages of the study (p < 0.05), and only BW was not correlated with insulin during exercise training and de-training (p > 0.05). Conclusion These findings indicate that both AET and RET are useful in reducing levels of serum adipocytokines (TNF-α, leptin) in diabetic and non-diabetic rats. At the same time, 4 weeks of de-training was sufficient to lose the metabolic adaptations.

scholarly journals Pleurotus tuber-regiumPolysaccharides Attenuate Hyperglycemia and Oxidative Stress in Experimental Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Hui-Yu Huang ◽  
Mallikarjuna Korivi ◽  
Ying-Ying Chaing ◽  
Ting-Yi Chien ◽  
Ying-Chieh Tsai
Keyword(s):  
Oxidative Stress ◽  
Serum Insulin ◽  
Glycosylated Hemoglobin ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Antioxidant Properties ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
High Fat ◽  
And Oxidative Stress

Pleurotus tuber-regiumcontains polysaccharides that are responsible for pharmacological actions, and medicinal effects of these polysaccharides have not yet been studied in diabetic rats. We examined the antidiabetic, antihyperlipidemic, and antioxidant properties ofP. tuber-regiumpolysaccharides in experimental diabetic rats. Forty rats were equally assigned as diabetic high-fat (DHF) diet and polysaccharides treated DHF groups (DHF+1P, DHF+2P, and DHF+3P, 20 mg/kg bodyweight/8-week). Diabetes was induced by chronic low-dose streptozotocin injections and a high-fat diet to mimic type 2 diabetes. Polysaccharides (1P, 2P, and 3P) were extracted from three different strains ofP. tuber-regium. Fasting blood glucose and glycosylated hemoglobin (HbA1c) levels substantially decreased, while serum insulin levels were restored by polysaccharides treatment compared to DHF. Furthermore, plasma total cholesterol, triglycerides, and low-density lipoprotein levels were significantly(P<0.01)lower in polysaccharide groups. High-density lipoprotein levels were attenuated with polysaccharides against diabetes condition. Polysaccharides inhibited(P<0.01)the lipid peroxidation index (malondialdehyde), and restored superoxide dismutase and glutathione peroxidase activities in the liver of diabetic rats. The antihyperglycemic property of polysaccharides perhaps boosts the antioxidant system that attenuates oxidative stress. We emphasize thatP. tuber-regiumpolysaccharides can be considered as an alternative medicine to treat hyperglycemia and oxidative stress in diabetic rats.

Sequoyitol ameliorates diabetic nephropathy in diabetic rats induced with a high-fat diet and a low dose of streptozotocin

2014 ◽  
Vol 92 (5) ◽  
pp. 405-417 ◽  
Author(s):  
Xian-Wei Li ◽  
Yan Liu ◽  
Wei Hao ◽  
Jie-Ren Yang
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Fat

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

Effect of the Joint Administration of Selenium and Vitamin E in Combination with Regular Aerobic Exercise on Markers of Lipid Peroxidation and Glutathione Peroxidase in Diabetic Rats

2005 ◽  
Vol 15 (3) ◽  
pp. 266-278 ◽  
Author(s):  
Hyun-Tae Kim
Keyword(s):  
Vitamin E ◽  
Exercise Training ◽  
Aerobic Exercise ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Acute Effect ◽  
Exercise Group ◽  
Diabetic Rats ◽  
Aerobic Exercise Training ◽  
Long Term Treatment

We investigated the effect of long-term treatment (6 wk) with selenium and vitamin E, in combination with aerobic exercise training, on malondialdehyde (MDA), oxidized low-density lipoprotein (ox-LDL), and glutathione peroxi-dase (GPx) in STZ-induced diabetic rats. The rats were assigned randomly to one of three treatment groups (n = 12 per group): 1) exercise group (EX), 2) selenium/vitamin E/exercise group (SVE), and 3) selenium/vitamin E group (SV). To estimate the acute effect of exercise, a 30-min endurance exercise was used. The MDA concentration was significantly lower in the SVE. The ox-LDL was significantly lower in the SVE and SV. The hepatic concentrations of selenium and vitamin E were significantly higher in the SVE. These results indicate that the increase in MDA is mildly attenuated in rats that were aerobically trained. Moreover, the joint administration of selenium and vitamin E with or without exercise training reduces the levels of ox-LDL.

scholarly journals Therapeutic Effects of 5,7-Dihydroxy-6-Oxoheptadecanoic Acid on Dysglycemia, Dyslipidemia, and Other Complications in Diabetic Rats

2020 ◽  
Vol 15 (7) ◽  
pp. 1934578X2093720
Author(s):  
Cuilan An ◽  
Lingling Wang ◽  
Yongli Liu ◽  
Emmanuel Ayobami Makinde ◽  
Huilian Li ◽  
...  
Keyword(s):  
Liver Function ◽  
Serum Insulin ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Insulin Level ◽  
Diabetic Rats ◽  
The Body ◽  
Antioxidant Enzyme Activities ◽  
Therapeutic Effects

The current study aimed to investigate the therapeutic effects of 5,7-dihydroxy-6-oxoheptadecanoic acid (DHA) from Tiliacora triandra on rat models of type 2 diabetes mellitus (T2DM). T2DM was induced with a combination of high-fat diet/streptozotocin (HFD/STZ), and diabetic rats were treated with DHA (25 mg/kg) for 30 days. The body weight, fasting blood glucose (FBG), serum, and liver biochemical parameters, as well as histological evaluations of the liver and pancreas, were evaluated. Diabetic rats displayed a significant increase in FBG, serum lipid profiles (triglycerides, total cholesterol, and low-density lipoprotein cholesterol), liver function enzymes (aspartate transaminase, alkaline phosphatase, and alanine transaminase), creatinine, liver malondialdehyde (MDA), and myeloperoxidase (MPO) contents. Furthermore, insulin level and liver antioxidant enzyme activities (catalase [CAT], superoxide dismutase [SOD], and glutathione peroxidase [GSH-Px]) were significantly reduced in the diabetic rats. Whereas, treatment with DHA significantly reduced FBG, serum lipids, liver function enzymes, serum creatinine, liver MDA, and MPO contents. In addition, treatment with DHA significantly increased serum insulin level and liver SOD, CAT, and GSH-Px activities. In addition, DHA alleviated histopathological changes in the pancreas and liver caused by T2DM. These results portray the antidiabetic and antioxidative properties of DHA and can be considered as a potential treatment for T2DM.

scholarly journals Bilobalide Enhances AMPK Activity to Improve Liver Injury and Metabolic Disorders in STZ-Induced Diabetes in Immature Rats via Regulating HMGB1/TLR4/NF-κB Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Meng Zhao ◽  
Jianpin Qin ◽  
Wenting Shen ◽  
Aiping Wu
Keyword(s):  
Liver Injury ◽  
Serum Insulin ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Diabetic Rats ◽  
Compound C ◽  
Group I ◽  
Immature Rats

This study was aimed at examining the effect and underlying mechanisms of bilobalide (BB) on hepatic injury in streptozotocin- (STZ-) induced diabetes mellitus (DM) in immature rats. Immature rats (one day old) were randomly divided into five groups: group I, control nondiabetic rats; group II, STZ-induced, untreated diabetic rats; groups III/IV/V, STZ-induced and BB-treated diabetic rats, which were intraperitoneally injected with BB (2.5 mg/kg, 5 mg/kg, or 10 mg/kg) after 3 days followed by STZ treatment. We observed that BB improved the histopathological changes and maintained normal glucose metabolism, blood lipid, and liver function indicators, such as fasting blood glucose, obesity index, HbA1c, HOMA-IR, fast serum insulin, adiponectin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate transaminase (AST), and alanine transaminase (ALT) in STZ-induced DM in immature rats by a biochemical analyzer or ELISA. Meanwhile, Western blot analysis showed that in STZ-induced DM immature rats, BB decreased the expression of apoptosis-related proteins Bax, cleaved caspase-3, and cleaved caspase-9 while enhancing the Bcl-2 expression; BB downregulated the expression of ACC related to fat anabolism, while upregulating the expression of CPT-1 related to fat catabolism. Strikingly, treatment with BB significantly increased the expression of AMPKα1 as well as inhibited HMGB1, TLR4, and p-P65 expression in hepatic tissues of immature DM rats. AMPK inhibitor (compound C, CC) cotreated with BB undermined the protective effect of BB on the liver injury. The results of the present study suggested BB may have a significant role in alleviating liver damage in the STZ-induced immature DM rats.

Abstract 17235: Attenuation of NPR-C Binding With Muscle-Derived Musclin is Related to Reduced Cardiovascular Disease Risks by Aerobic Exercise Training in Type 2 Diabetic Rats

Circulation ◽  
2018 ◽  
Vol 138 (Suppl_1) ◽  
Author(s):  
Yuta Toyama ◽  
Natsuki Hasegawa ◽  
Naoki Horii ◽  
Kenichiro Inoue ◽  
Keiko Iemitsu ◽  
...  
Keyword(s):  
Exercise Training ◽  
Aerobic Exercise ◽  
Serum Insulin ◽  
Diabetic Rats ◽  
Aerobic Exercise Training ◽  
Glut4 Translocation ◽  
Sedentary Control ◽  
C Protein ◽  
Type 2 Diabetic

Introduction: Aerobic exercise training (AT) in type 2 diabetic patients reduces cardiovascular disease (CVD) risks, such as an impairment of NO-derived vasodilation mediated by obese and hyperinsulinemia. Musclin is a muscle-derived myokine and binds to natriuretic peptide receptor-C (NPR-C). Elevation of circulating musclin level deteriorates of insulin resistance. Furthermore, musclin-induced vasocontraction mediated by NPR-C leads to increase in blood pressures. However, the effect of AT on muscle-derived musclin secretion in type 2 diabetes remains unclear. Hypothesis: This study aimed to clarify whether AT-induced attenuations of muscle-derived musclin secretion and arterial NPR-C expression levels are related to reduction of CVD risks in type 2 diabetes rats. Methods: Twenty 20-week-old male type 2 diabetic (OLETF) rats were randomly divided into two groups; 8-week sedentary control and aerobic exercise training (treadmill running for 60min at 25m/min, 5days/week) (n=7 each group) and seven male LETO rats used as healthy sedentary control. After 8 weeks, we measured carotid-femoral pulse wave velocity (cfPWV, an index of arterial stiffness), QUICKI (an index of insulin sensitivity), plasma and muscle musclin levels and GLUT4 translocation levels, arterial NPR-C protein expression. Results: In sedentary diabetic rats, cfPWV, serum insulin and muscle musclin levels, and arterial NPR-C protein expression increased, and QUICKI decreased as compared to healthy sedentary rats (each p<0.05). However, no significant circulating musclin level between sedentary and exercised diabetic rats was observed. In contrast, aerobic exercise training in the diabetic rats induced elevation of QUICKI and reduction of cfPWV, serum insulin, muscle musclin, and arterial NPR-C levels (each p<0.05). NPR-C protein level was positively correlated with cfPWV (r=0.43, p=0.09). Additionally, muscle musclin levels was significantly negatively correlated with muscle GLUT4 translocation (r=-0.78, p<0.01) and QUICKI (r=-0.82, p<0.01). Conclusions: These results suggest that attenuation of arterial NPR-C expression and muscle-derived musclin secretion is related to reductions of arterial stiffness and hyperinsulinemia by AT in type 2 diabetic rats.

scholarly journals Hypoglycemic Activity and Antioxidative Stress of Extracts and Corymbiferin fromSwertia bimaculata In VitroandIn Vivo

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Zhaoxia Liu ◽  
Luosheng Wan ◽  
Yuedong Yue ◽  
Zuoqi Xiao ◽  
Yutang Zhang ◽  
...  
Keyword(s):  
Serum Insulin ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Glucose Consumption ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Alternative Agent

The present study was to investigate the anti-diabetic activities ofSwertia bimaculata. Based on the glucose consumption ofS. bimaculataextractsand different fractions (petroleum, dichloromethane, ethyl acetate,n-butanol and water extracts) in 3T3-L1 adipocyte assay, ethanol (ETH) and dichloromethane (DTH) extracts had the most effective potency. Furthermore, ETH, DTH and corymbiferin (the most abundant component of DTH) were evaluated for anti-diabetic effects in high fat and sucrose fed combined with low dose streptozocin induced diabetic rats. DTH and corymbiferin displayed remarkable anti-diabetic activities. The fasting blood glucose levels were significantly decreased, while the serum insulin levels were obviously increased. The oral glucose tolerance was also improved. The lowed serum total cholesterol, low density lipoprotein (LDL) and triglyceride levels and increased ratio of HDL (high density lipoprotein)/LDL were observed. The insulin sensitivity was improved on the basis of increased expressions of insulin-receptor substrate-2, phosphatidylinositol 3-kinase and Ser/Thr kinase AKT2. And also DTH and corymbiferin improved antioxidant capacity and carbohydrate metabolism in diabetic rats, along with the improvement of histopathology of livers and pancreaticβcells. Corymbiferin was one of active constituents, responsible for anti-diabetic properties. Therefore,S. bimaculatacould be considered as an alternative agent against diabetes mellitus.

Hyperbaric oxygen therapy attenuates D-galactose-induced-age-related cardiac dysfunction through mitigating cardiac mitochondrial dysfunction in pre-diabetic rats

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
C Bo-Htay ◽  
T Shwe ◽  
S Palee ◽  
T Pattarasakulchai ◽  
K Shinlapawittayatorn ◽  
...  
Keyword(s):  
Mitochondrial Dysfunction ◽  
High Fat Diet ◽  
Diabetic Rats ◽  
Normal Diet ◽  
Lv Function ◽  
High Fat ◽  
Insulin Resistant ◽  
Lv Dysfunction ◽  
Age Related ◽  
Mitochondrial Functions

Abstract Background D-galactose (D-gal) induced ageing has been shown to exacerbate left ventricular (LV) dysfunction via worsening of apoptosis and mitochondrial dysfunction in the heart of obese rats. Hyperbaric oxygen therapy (HBOT) has been demonstrated to exert anti-inflammatory and anti-apoptotic effects in multiple neurological disorders. However, the cardioprotective effect of HBOT on inflammation, apoptosis, LV and mitochondrial functions in D-gal induced ageing rats in the presence of obese-insulin resistant condition has never been investigated. Purpose We sought to determine the effect of HBOT on inflammation, apoptosis, mitochondrial functions and LV function in pre-diabetic rats with D-gal induced ageing. We hypothesized that HBOT attenuates D-gal induced cardiac mitochondrial dysfunctions and reduces inflammation and apoptosis, leading to improved LV function in pre-diabetic rats. Methods Forty-eight male Wistar rats were fed with either normal diet or high-fat diet for 12 weeks. Then, rats were treated with either vehicle groups (0.9% NSS, subcutaneous injection (SC)) or D-gal groups (150 mg/kg/day, SC) for 8 weeks. At week 21, rats in each group were equally divided into 6 sub-groups: normal diet fed rats treated with vehicle (NDV) sham, normal diet fed rats treated with D-gal (NDDg) sham, high fat diet fed rats treated with D-gal (HFDg) sham, high fat diet fed rats treated with vehicle (HFV) + HBOT, NDDg + HBOT and HFDg + HBOT. Sham treated rats were given normal concentration of O2 (flow rate of 80 L/min, 1 ATA for 60 minutes), whereas HBOT treated rats were subjected to 100% O2 (flow rate of 250 L/min, 2 ATA for 60 minutes), given once daily for 2 weeks. Results Under obese-insulin resistant condition, D-gal-induced ageing aggravated LV dysfunction (Fig 1A) and impaired cardiac mitochondrial function, increased cardiac inflammatory and apoptotic markers (Fig 1B). HBOT markedly reduced cardiac TNF-α level and TUNEL positive apoptotic cells, and improved cardiac mitochondrial function as indicated by decreased mitochondrial ROS production, mitochondrial depolarization and mitochondrial swelling, resulting in the restoration of the normal LV function in HFV and NDDg rats, compared to sham NDDg rats. In addition, in HFDg treated rats, HBOT attenuated cardiac TNF-α level, TUNEL positive apoptotic cells and cardiac mitochondrial dysfunction, compared to sham HFDg rats, leading to improved cardiac function as indicated by increased %LV ejection fraction (LVEF) (Figure 1). Conclusion HBOT efficiently alleviates D-gal-induced-age-related LV dysfunction through mitigating inflammation, apoptosis and mitochondrial dysfunction in pre-diabetic rats. Figure 1 Funding Acknowledgement Type of funding source: Public grant(s) – National budget only. Main funding source(s): 1. The National Science and Technology Development Agency Thailand, 2. Thailand Research Fund Grants

scholarly journals APOE genotype affects black-white responses of high-density lipoprotein cholesterol subspecies to aerobic exercise training

Metabolism ◽  
2008 ◽  
Vol 57 (12) ◽  
pp. 1669-1676 ◽  
Author(s):  
Thomas O. Obisesan ◽  
Robert E. Ferrell ◽  
Andrew P. Goldberg ◽  
Dana A. Phares ◽  
Tina J. Ellis ◽  
...  
Keyword(s):  
Exercise Training ◽  
High Density Lipoprotein ◽  
Aerobic Exercise ◽  
High Density Lipoprotein Cholesterol ◽  
Apoe Genotype ◽  
Density Lipoprotein ◽  
High Density ◽  
Aerobic Exercise Training ◽  
Lipoprotein Cholesterol

scholarly journals Serum Apelin and Obesity-Related Complications in Egyptian Children

2018 ◽  
Vol 6 (8) ◽  
pp. 1354-1358 ◽  
Author(s):  
Maged A. El Wakeel ◽  
Ghada M. El-Kassas ◽  
Alyaa H. Kamhawy ◽  
Essam M. Galal ◽  
Maysa S. Nassar ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Childhood Obesity ◽  
Serum Insulin ◽  
Low Density Lipoprotein ◽  
Linear Regression Analysis ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Z Score ◽  
Obese Children ◽  
Egyptian Children

BACKGROUND: The rapidly increasing prevalence of childhood obesity became a major burden on health worldwide, giving an alarm to clinicians and researchers. Adipocytes act as an active endocrine organ by releasing plenty of bioactive mediators (adipokines) that play a major role in regulating metabolic processes. Apelin is a recently identified adipokine that is expressed in adipocytes.AIM: The current work aimed to uncover the relation between serum apelin and childhood obesity and its related complications as hypertension and hyperglycemiaMETHOD: A group of 50 obese and 31 non-obese; sex- and age-matched children were enrolled in our study with a mean age of (9.5 ± 2.1) and (8.7 ± 1.3) respectively. Anthropometric measurements, blood pressure, were assessed in all studied participants, we also determined the lipid profile, serum insulin, fasting blood glucose (FBG) level, HOMA-IR and serum apelin.RESULTS: Obese children had higher levels of HbA1c, FBG, serum insulin, HOMA-IR, total cholesterol, triglycerides, low-density lipoprotein (LDL) and diastolic blood pressure (DBP Z-score); compared to controls (all P < 0.05). Apelin was significantly higher in obese children versus controls and correlated positively with BMI Z-Score (P = 0.008), DBP Z-Score (P = 0.02), cholesterol, TG (both P = 0.02), serum insulin (P = 0.003), FBG and HOMA-IR (both P = 0.001). Linear regression analysis showed that FBG was the most effective factor in predicting the level of serum apelin (P = 0.04).CONCLUSION: This work supports the hypothesis that apelin may have a crucial role in the pathogenesis of health hazards related to obesity in children including insulin resistance, hypertension and a higher risk of occurrence of metabolic syndrome.

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