scholarly journals Protocol optimization for a fast, simple and economical chemical reduction synthesis of antimicrobial silver nanoparticles in non-specialized facilities

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Roberto Vazquez-Muñoz ◽  
M. Josefina Arellano-Jimenez ◽  
Fernando D. Lopez ◽  
Jose L. Lopez-Ribot
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Reduction Process ◽  
Cost Effective ◽  
Silver Nitrate Solution ◽  
Synthesis Time ◽  
Average Size

Abstract Objective Silver nanoparticles (AgNPs) can be difficult or expensive to obtain or synthesize for laboratories in resource-limited facilities. The purpose of this work was to optimize a synthesis method for a fast, facile, and cost-effective synthesis of AgNPs with antimicrobial activity, which can be readily implemented in non-specialized facilities and laboratories. Results The optimized method uses a rather simple and rapid chemical reduction process that involves the addition of a polyvinylpyrrolidone solution to a warmed silver nitrate solution under constant vigorous stirring, immediately followed by the addition of sodium borohydride. The total synthesis time is less than 15 min. The obtained AgNPs exhibit an aspect ratio close to 1, with an average size of 6.18 ± 5 nm. AgNPs displayed potent antimicrobial activity, with Minimal Inhibitory Concentration values of ≤ 4 µg mL−1 for Staphylococcus aureus and ≤ 2 µg mL−1 for Candida albicans. The resulting method is robust and highly reproducible, as demonstrated by the characterization of AgNPs from different rounds of syntheses and their antimicrobial activity.

scholarly journals A fast and simple protocol for synthesizing effective antimicrobial silver nanoparticles in non-specialized facilities

2019 ◽  
Author(s):  
Roberto Vazquez-Munoz ◽  
M. Josefina Arellano-Jimenez ◽  
Jose L. Lopez-Ribot
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Chemical Reduction ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Antimicrobial Properties ◽  
Reduction Process ◽  
Cost Effective ◽  
Silver Nitrate Solution ◽  
Average Size

Abstract Objective Silver nanoparticles (AgNPs) can be difficult or expensive to obtain or synthesize for laboratories in resource-limited facilities. The purpose of this work was to create a fast, facile, and cost-effective method for synthesizing AgNPs with potent antimicrobial properties, that can be readily implemented in non-specialized laboratories.Results Our developed method uses a rather simple and rapid chemical reduction process that involves the addition of a polyvinylpyrrolidone solution to a warmed silver nitrate solution under constant vigorous stirring, immediately followed by the addition of sodium borohydride with constant stirring for an additional 15 minutes. AgNPs had an aspect ratio close to 1, with an average size of 6.18 ± 5 nm. AgNPs displayed potent antimicrobial activity, with Minimal Inhibitory Concentration values of 3 µg mL-1 and 1.5 µg mL-1 for Staphylococcus aureus and Candida albicans respectively.Keywords : Silver nanoparticles, nanoantibiotics, synthesis method, AgNPs, metallic nanoparticles

Phytochemical Synthesis of Silver Nanoparticles Using Anthemis Nobilis Extract and Its Antibacterial Activity

2020 ◽  
Vol 234 (3) ◽  
pp. 531-540
Author(s):  
Saba Ghamipoor ◽  
Faeze Fayyazi ◽  
Saeed Bahadorikhalili
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Silver Ions ◽  
Good Alternative ◽  
Ft Ir ◽  
Average Size ◽  
Physical And Chemical

AbstractIn this work, green synthesis of silver nanoparticles is described by phytochemical reducing silver nitrate aqueous solution using Anthemis nobilis. For this purpose, Anthemis nobilis extract was used for the synthesis of silver nanoparticles as both surfactant and reducing agent. Green synthesis method is a good alternative to physical and chemical methods, since it is fast, simple, environmentally-friendly and economic. The produced nanoparticles are identified using FE-SEM, EDX, and FT-IR and Uv/Vis techniques. Formation of silver nanoparticles is verified in 430–420 nm range. Reduction of silver ions by hydroxyl functional group is also confirmed by FT-IR device. EDX device confirms the presence of a peak for Ag element without any impurity peak. Silver nanoparticles are identified by FE-SEM device and found to have average size between 17 and 42 nm. Also, the antibacterial activity of the synthesized nanoparticles is compared with that of staphyloccusaureus and pseudomonasa aeruginosa and the maximum inhibitory activity against the bacteria is obtained using 1 mM nitrate solution.

scholarly journals Biosynthesis of Silver Nanoparticles Using Ligustrum Ovalifolium Fruits and Their Cytotoxic Effects

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 627 ◽  
Author(s):  
Bianca Moldovan ◽  
Vladislav Sincari ◽  
Maria Perde-Schrepler ◽  
Luminita David
Keyword(s):  
Silver Nanoparticles ◽  
Synthesis Method ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Energetic Cost ◽  
Fruit Extract ◽  
Vis Spectroscopy ◽  
Biosynthesized Agnps ◽  
Average Size

The present study reports for the first time the efficacy of bioactive compounds from Ligustrum ovalifolium L. fruit extract as reducing and capping agents of silver nanoparticles (AgNPs), developing a green, zero energetic, cost effective and simple synthesis method of AgNPs. The obtained nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR), confirming that nanoparticles were crystalline in nature, spherical in shape, with an average size of 7 nm. The FTIR spectroscopy analysis demonstrated that the AgNPs were capped and stabilized by bioactive molecules from the fruit extract. The cytotoxicity of the biosynthesized AgNPs was in vitro evaluated against ovarian carcinoma cells and there were found to be effective at low concentration levels.

scholarly journals Photocatalytic Reduction of Methyl Orange, Antibacterial and Antibreast Cancer Activities of Biogenic Silver Nanoparticle Synthesized from Beta vulgaris Extract

2021 ◽  
Vol 33 (12) ◽  
pp. 2993-2998
Author(s):  
F. Femina ◽  
H. Asia Thabassoom ◽  
J. Felicita Florence
Keyword(s):  
Silver Nanoparticles ◽  
Methyl Orange ◽  
Beta Vulgaris ◽  
Anticancer Agents ◽  
Reduction Process ◽  
Cost Effective ◽  
Xrd Analysis ◽  
Photocatalytic Reduction ◽  
Hazardous Chemical ◽  
Average Size

Studies based on a biogenic synthesis of noble metal nanomaterials has become a promising one in today’s biomedical approach in therapy and diagnosis due to multidimensional applications. Hence, the present study is to explore the antibacterial, antibreast cancer and photocatalytic efficacy of silver nanoparticles (AgNPs) synthesized from an extract of Beta vulgaris (BV). The band at 398 nm in the UV-visible spectra confirmed the formation of AgNPs. The characteristic shift in OH and C=O peak after the formation of silver nanoparticles shows the participation of Beta vulgaris extracts in the reduction process which is further supported by from SEM morphology. The average size of the particle (17 nm) was determined from XRD analysis using Scherrer’s equation. Antibacterial results of Beta vulgaris mediated BV-AgNPs show the maximum zone of inhibition against Candida albicans. On anticancer activity, BV-AgNPs reveals the toxicity effect on the MCF-7 cell line with an IC50 value of 40.65 μg/mL. Similarly, it reduces 81.3% of methyl orange at 180 min on the photocatalytic reduction process. This study has suggested an effective replacement for the hazardous chemical methods and leads to a cost-effective, environmentally-friendly method that can also be used as antibacterial and anticancer agents.

Ag Nanoparticles Adsorption on Diatom-Montmorillonite Clays

2013 ◽  
Vol 755 ◽  
pp. 91-96
Author(s):  
A. Ruíz-Baltazar ◽  
R. Esparza ◽  
R. Pérez ◽  
G. Rosas
Keyword(s):  
Silver Nanoparticles ◽  
Porous Material ◽  
Ag Nanoparticles ◽  
Diatomaceous Earth ◽  
Chemical Reduction ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
X Ray Diffraction ◽  
Montmorillonite Clays ◽  
Complementary Techniques

In this work, a spectroscopic and structural characterization of diatomaceous earth-montmorillonite clays after impregnated with silver nanoparticles were carried out. The silver nanoparticles were synthesized by chemical reduction with sodium borohydride starting from silver nitrate solution. The obtained nanoparticles were stabilized with polyvinyl-pyrrolidone as a surface agent. In order to perform the homogeneous nucleation process, Ag nanoparticles solutions at concentrations of 1, 2 and 4 parts per million were magnetically mixed in the porous material. Additionally, we assessed the porous material adsorption ability of silver by atomic absorption spectroscopy. The quantity of Ag nanoparticles adsorbed by the diatomaceous earth and the adsorption rate as function of the concentration of Ag nanoparticles were established. Other complementary techniques such as X-ray diffraction, infrared spectroscopy and transmission and scanning electron microscopy were used.

scholarly journals RADIATION SYNTHESIS OF SILVER NANOPARTICLES/CHITOSAN AGAINST Corynespora cassiicola CAUSING LEAF FALL DISEASE ON RUBBER TREES

2018 ◽  
Vol 55 (1A) ◽  
pp. 45
Author(s):  
Le Thi An Nhien
Keyword(s):  
Silver Nanoparticles ◽  
Nitrate Solution ◽  
Average Particle Size ◽  
Silver Nitrate Solution ◽  
Average Particle ◽  
In Vitro Test ◽  
Corynespora Cassiicola ◽  
Size Of Particles ◽  
Average Size

In this study, silver nanoparticles (AgNPs) were prepared by gamma rays irradiation of 1.0, 2.5, 5.0 and 10 mM silver nitrate solution using chitosan as a stabilizer. UV spectra, morphology and size of AgNPs irradiated at different doses were characterized by using UV-vis spectrophotometer and TEM images. The obtained results indicated that the average size of AgNPs increased by the increase of silver concentration in irradiated solution or the degree of acetylation of chitosan, while the increase of chitosan concentration was found to be a functional key for reducing the average size of particles in AgNPs product. In vitro test, AgNPs inhibited the growth of Corynespora cassiicola. In particularly, the inhibitory efficiency of AgNPs on the growth of C. cassiicola on rubber leaf extract media increased from 52.1 to 100 % when the average particle size of particles in AgNPs product decreased from 15 to 5 nm at the concentration of 50 ppm. In addition, the increase of AgNPs concentration from 10 to 90 ppm also enhanced the antifungal activity to be from 6.3 to 100 %, respectively. It suggests that the silver nanoparticles/chitosan (AgNPs/chitosan) synthesized by γ-rays irradiation method is a very promising fungicidal product applying for treating C. cassiicola, a serious pathogen fungus on rubber trees.

scholarly journals Sintesis dan karakterisai kitosan dari limbah cangkang udang sebagai stabilizer terhadap Ag nanopartikel

2019 ◽  
Vol 4 (2) ◽  
pp. 86
Author(s):  
Astuti Amin ◽  
Nur Khairi ◽  
Eko Allo
Keyword(s):  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Nitrate Solution ◽  
Average Particle Size ◽  
Silver Nitrate Solution ◽  
Raw Material ◽  
Average Particle ◽  
Synthesize Silver Nanoparticles ◽  
Shrimp Shell ◽  
Shell Powder

The research of manufacturing chitosan from shrimp shell waste , and their use as a stabilizer in the manufacture of silver nanoparticles has been done. The aim of the research was to synthesize silver nanoparticles using chitosan as a stabilizer by chemical reduction method and determine the effect of chitosan concentration on the stability of Ag nanoparticles. In this study, the raw material used is shrimp shell powder and then processed in several stages, eliminating proteins, demineralization, and deacetylation. Chitosan obtained is 16.4 % of shrimp shell powder, with a degree of deacetylation of 85 %. Chitosan is used to synthesize silver nanoparticles as a reducing agent of silver ions in silver nitrate solution and is expected to be stabilizer. Sample containing 45 mg of chitosan and 1000 ppm AgNO3 has 421,60 nm of maximum wavelength, and the average particle size is 154.07 nm.

Silver Nanoparticles by Amino-Terminated Hyperbranched Polymer : Characterization and Antimicrobial Property on Cotton

2011 ◽  
Vol 332-334 ◽  
pp. 930-934
Author(s):  
Hong Lin ◽  
Ling Chen ◽  
De Suo Zhang ◽  
Yu Yue Chen
Keyword(s):  
Silver Nanoparticles ◽  
Hyperbranched Polymer ◽  
Antimicrobial Agents ◽  
Antimicrobial Property ◽  
Nitrate Solution ◽  
Analytical Techniques ◽  
Silver Nitrate Solution ◽  
The Stability ◽  
Average Size ◽  
And Staphylococcus Aureus

A hyperbranched polymer (HBP-NH2) acting as a reducing agent and stablizer agent has been synthesized to investigate the stability of silver nanoparticles antimicrobial agent, which were prepared in a hyperbranched polymer matrix by a reaction with silver nitrate solution. The antimicrobial agents generated were characterized by different spectroscopic and analytical techniques such as DLS, TEM and UV-vis, which confirm the formation of well-dispersed silver nanoparticles with average size around 12.0 nm. Furthermore, antimicrobial and mechanical properties of nanosilver treated cotton fabrics were investigated. The results showed that stable silver nanoparticles collide solution with 12 nm, applied on cotton fabric under certain condition, could produce ideal antibacterial rate over 94% against both Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) even after 50 consecutive washings.

scholarly journals Antimicrobial Activity of Biogenic Hop-Based Silver Nanoparticles for Application in Sugar and Alcohol Industries

2019 ◽  
pp. 277-286 ◽  
Author(s):  
Thais Castro Borsari ◽  
Tais Germano-Costa ◽  
Natalia Bilesky-Jose ◽  
Leonardo F. Fraceto ◽  
Renata Lima
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Bactericidal Activity ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Mitochondrial Activity ◽  
Alcohol Production ◽  
Flower Extract ◽  
Physical And Chemical ◽  
Biogenic Nanoparticles

One of the largest problems in the sugar and alcohol industry is the presence of unwanted bacteria, which leads to a decrease in alcohol production. This work presents a possible way to control these undesirable bacteria using silver biogenic nanoparticles synthesized from hops. The research was aimed at synthesizing nanoparticles and evaluating their physical and chemical characteristics, antimicrobial activity and cytotoxic potential. Silver nanoparticles were synthesized using Humulus lupulus L. extract and silver nitrate solution (10-3 M). To evaluate the size and polydispersity, the dynamic light scattering (DLS) technique was used. The zeta potential and concentration of nanoparticles were assessed by microelectrophoresis and the nanoparticle tracing (NTA) technique, respectively. The analysis of the antimicrobial activity was performed by the disc diffusion test and by determining the minimum inhibitory concentration (MIC) against gram-positive bacteria. Cytotoxicity was assessed by the mitochondrial activity assay (MTT). The biogenic nanoparticles were shown to have bactericidal activity and the appropriate characteristics. Regarding the activity, the results showed that the nanoparticles obtained at pH 7 (with 5% hop flower extract) inhibited all tested bacteria at concentrations of 2.1 to 4.3 × 109 NP/mL, and the nanoparticles obtained at pH 8 (with 20% hop flower extract) inhibited the tested bacteria at concentrations of 4.8 to 6.0 × 109 NPs/mL, which means that the former presented higher potential bactericidal activity. Regarding cytotoxicity, nanoparticles of both types showed a low cell death index, and the cell viability was above 70%. Hops served as an effective stabilizing and reducing agent. The nanoparticles were effective in bacterial control and did not show cytotoxicity

scholarly journals Biosynthesis of Silver nanoparticles using Desmodium Gangeticum leaf extract

2019 ◽  
Vol 10 (4) ◽  
pp. 3711-3716
Author(s):  
Niladry Sekhar Ghosh ◽  
Ritu M Giilhotra ◽  
Ranjit Singh ◽  
Angshu Banerjee
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Short Interval ◽  
Synthesis Method ◽  
Silver Ions ◽  
Cost Effective ◽  
Environment Friendly ◽  
Crystalline Nature ◽  
Average Size ◽  
Short Time

A range of methods is available for the synthesize nanoparticles. However, these methods are associated with the production of undesirable byproducts which are quite hazardous and high costs. Thus a number of efforts are being made to develop novel cost-effective safe & reliable "green" procedures which caned produce desired nanoparticles. In this study, we caned successfully develop a green synthesis method for preparation of silver nanoparticles using Desmodium gangeticum leaf extract as reducing & capping agents. The method was found a quite effective inversion of silver ions to silver nanoparticles in a short interval of time. The developed nanoparticles exhibited Surface plasmon resonance at around 500 nm. The particles are nearly spherical, and the size ranged between 16-64nm. The average size was noted to be around 40 nm. The nanoparticles were characterized for their morphology using UV-vis, TEM, FTIR analysis and FESEM. The developed method carries the advantage of the completion of the reaction in a short time. The crystalline nature of the synthesize nanoparticles was assessed & confirm by XRD & EDX Studies. From FTIR studies, it can be understood that the flavonoids could be adsorbed on the metal surface by interaction with carbonyl groups. The process was carried out in the environment-friendly condition.

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