Antimicrobial Activity of Biogenic Hop-Based Silver Nanoparticles for Application in Sugar and Alcohol Industries

One of the largest problems in the sugar and alcohol industry is the presence of unwanted bacteria, which leads to a decrease in alcohol production. This work presents a possible way to control these undesirable bacteria using silver biogenic nanoparticles synthesized from hops. The research was aimed at synthesizing nanoparticles and evaluating their physical and chemical characteristics, antimicrobial activity and cytotoxic potential. Silver nanoparticles were synthesized using Humulus lupulus L. extract and silver nitrate solution (10-3 M). To evaluate the size and polydispersity, the dynamic light scattering (DLS) technique was used. The zeta potential and concentration of nanoparticles were assessed by microelectrophoresis and the nanoparticle tracing (NTA) technique, respectively. The analysis of the antimicrobial activity was performed by the disc diffusion test and by determining the minimum inhibitory concentration (MIC) against gram-positive bacteria. Cytotoxicity was assessed by the mitochondrial activity assay (MTT). The biogenic nanoparticles were shown to have bactericidal activity and the appropriate characteristics. Regarding the activity, the results showed that the nanoparticles obtained at pH 7 (with 5% hop flower extract) inhibited all tested bacteria at concentrations of 2.1 to 4.3 × 109 NP/mL, and the nanoparticles obtained at pH 8 (with 20% hop flower extract) inhibited the tested bacteria at concentrations of 4.8 to 6.0 × 109 NPs/mL, which means that the former presented higher potential bactericidal activity. Regarding cytotoxicity, nanoparticles of both types showed a low cell death index, and the cell viability was above 70%. Hops served as an effective stabilizing and reducing agent. The nanoparticles were effective in bacterial control and did not show cytotoxicity

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Potential antibacterial activity of crude extracts and silver nanoparticles synthesized from Sargassum wightii

International Current Pharmaceutical Journal ◽

10.3329/icpj.v3i10.20337 ◽

2014 ◽

Vol 3 (10) ◽

pp. 322-325 ◽

Cited By ~ 5

Author(s):

Vinoth Kumar Thirumalairaj ◽

Mahitha Puthanpurayal Vijayan ◽

Geetharamani Durairaj ◽

Lakshmanasenthil Shanmugaasokan ◽

Rincy Yesudas ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Zone Of Inhibition ◽

Ag Nps ◽

Sargassum Wightii ◽

Solvent Extract ◽

Vis Spectroscopy

The present work investigates the antibacterial activity of silver nanoparticles (Ag-NPs) synthesized by biological method using Sargassum wightii. The fresh live seaweed was collected from the Mandapam coast of Tamilnadu, India. Solvent extract was prepared using acetone, petroleum ether and methanol. Aqueous extract of the seaweed was also used for the synthesis of silver Ag-NPs. Seaweed extract is used as a reducing agent of 2mM silver nitrate solution for the synthesis of Ag-NPs. Periodical monitoring of reaction mixture was done using UV-vis spectroscopy at 300-750 nm. The scanning electron microscopy (SEM) of the sample confirms the presence of Ag-NPs. The antibacterial activity of solvent extract was done by Minimal inhibitory concentration (MIC) assay. The methanol extract of the seaweed at a concentration of 250µg/ml exhibited potent antimicrobial activity against the test microorganism. The zone of inhibition ranging from 8-14 mm was observed with different extracts. The antibacterial activity of the synthesized Ag-NPs against the organism was also done by MIC test. The MIC of Ag-NPs was found to be 130µg/ml for all pathogenic microorganisms selected for the study. The zone of inhibition against Bacillus cereus, Bacillus anhtracis, Staphylococcus aureus and Vibrio alginoyticus were found to be 10, 8, 10 and 9 mm, respectively. The synthesized Ag-NPs exhibited significant antimicrobial activity against the selected microorganisms than the solvent extract of seaweed.DOI: http://dx.doi.org/10.3329/icpj.v3i10.20337 International Current Pharmaceutical Journal, September 2014, 3(10): 322-325

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Protocol optimization for a fast, simple and economical chemical reduction synthesis of antimicrobial silver nanoparticles in non-specialized facilities

BMC Research Notes ◽

10.1186/s13104-019-4813-z ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 9

Author(s):

Roberto Vazquez-Muñoz ◽

M. Josefina Arellano-Jimenez ◽

Fernando D. Lopez ◽

Jose L. Lopez-Ribot

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Chemical Reduction ◽

Nitrate Solution ◽

Synthesis Method ◽

Reduction Process ◽

Cost Effective ◽

Silver Nitrate Solution ◽

Synthesis Time ◽

Average Size

Abstract Objective Silver nanoparticles (AgNPs) can be difficult or expensive to obtain or synthesize for laboratories in resource-limited facilities. The purpose of this work was to optimize a synthesis method for a fast, facile, and cost-effective synthesis of AgNPs with antimicrobial activity, which can be readily implemented in non-specialized facilities and laboratories. Results The optimized method uses a rather simple and rapid chemical reduction process that involves the addition of a polyvinylpyrrolidone solution to a warmed silver nitrate solution under constant vigorous stirring, immediately followed by the addition of sodium borohydride. The total synthesis time is less than 15 min. The obtained AgNPs exhibit an aspect ratio close to 1, with an average size of 6.18 ± 5 nm. AgNPs displayed potent antimicrobial activity, with Minimal Inhibitory Concentration values of ≤ 4 µg mL−1 for Staphylococcus aureus and ≤ 2 µg mL−1 for Candida albicans. The resulting method is robust and highly reproducible, as demonstrated by the characterization of AgNPs from different rounds of syntheses and their antimicrobial activity.

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Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies

Green Processing and Synthesis ◽

10.1515/gps-2020-0024 ◽

2020 ◽

Vol 9 (1) ◽

pp. 283-293

Author(s):

Milad Torabfam ◽

Meral Yüce

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Chlorella Vulgaris ◽

Metallic Nanoparticles ◽

Nitrate Solution ◽

Spherical Shape ◽

Silver Nitrate Solution ◽

Spectroscopy Analysis ◽

Bacterial Diseases ◽

Serovar Typhimurium

AbstractGreen synthesis of metallic nanoparticles (NPs) is acquiring considerable attention due to its environmental and economic superiorities over other methods. This study describes the practical synthesis of silver nanoparticles (AgNPs) through the reduction of silver nitrate solution using an algal source, Chlorella vulgaris, as the reducing as well as the stabilizing agent. The energy required for this synthesis was supplied by microwave radiation. The ultraviolet-visible spectroscopy exhibited a single peak related to the surface plasmon absorbance of AgNPs at 431 nm. The AgNPs with high stability (a zeta potential of −17 mV), hydrodynamic size distribution of 1–50 nm, and mostly spherical shape were obtained through a 10 min process. Fourier transform infrared spectroscopy analysis revealed that several functional groups, including carbonyl groups of C. vulgaris, play a significant role in the formation of functional NPs. Antibacterial features of the produced AgNPs were verified against those of Salmonella enterica subsp. enterica serovar typhimurium and Staphylococcus aureus, demonstrating a considerable growth inhibition at increasing concentrations of the NPs. As a result, the formed AgNPs can be used as a promising agent against bacterial diseases.

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Antimicrobial Bacterial Cellulose-Silver Nanoparticles Composite Membranes

Journal of Nanomaterials ◽

10.1155/2011/721631 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 117

Author(s):

Hernane S. Barud ◽

Thaís Regiani ◽

Rodrigo F. C. Marques ◽

Wilton R. Lustri ◽

Younes Messaddeq ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Bacterial Cellulose ◽

Nitrate Solution ◽

Composite Membranes ◽

Silver Nitrate Solution ◽

Complexing Agent ◽

X Ray Diffraction ◽

Gram Positive Bacteria ◽

Transmission Electron

Antimicrobial bacterial cellulose-silver nanoparticles composite membranes have been obtained by“in situ”preparation of Ag nanoparticles from hydrolytic decomposition of silver nitrate solution using triethanolamine as reducing and complexing agent. The formation of silver nanoparticles was evidenced by the X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and absorption in the UV-Visible (350 nm to 600 nm). Thermal and mechanical properties together with swelling behavior for water were considered. TEA concentration was observed to be important in order to obtain only Ag particles and not a mixture of silver oxides. It was also observed to control particle size and amount of silver contents in bacterial cellulose. The composite membranes exhibited strong antimicrobial activity against Gram-negative and Gram-positive bacteria.

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Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Produced by Yarrowia lipolytica Against Vegetative and Starved Shigella

NANO ◽

10.1142/s1793292021500880 ◽

2021 ◽

pp. 2150088

Author(s):

Kalthoum Chourabi ◽

Lobna Elleuch ◽

Salma Kloula ◽

Ahmed Landoulsi ◽

Abdelwaheb Chatti

Keyword(s):

Silver Nanoparticles ◽

Yarrowia Lipolytica ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Water Disinfection ◽

Personal Hygiene ◽

Visible Range ◽

Significant Toxicity ◽

Air Disinfection ◽

The Stability

Silver nanoparticles have attracted much interest from scientists to develop nanosilver-based disinfectant products due to their unique properties of high antimicrobial activity. This study focused on biosynthesis, characterization, antimicrobial and antibiofilm effects of silver nanoparticles against vegetative and starved Shigella strains. The silver nanoparticles were synthesized using the yeast Yarrowia lipolytica and characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial and antibiofilm activities of silver nanoparticles were tested against the growth of vegetative and starved Shigella strains. After the addition of silver nitrate solution to the supernatant of Y. lipolytica, we noticed the appearance of a brown-black coloration that suggested the formation of silver nanoparticles. The presence of silver nanoparticles was manifested by a maximum absorption in the ultraviolet–visible range, precisely at the wavelength 420[Formula: see text]nm. The crystalline nature and the stability of silver nanoparticles were confirmed, respectively, by XRD and FTIR analysis. The antibacterial activity of silver nanoparticles showed significant toxicity on Shigella strains indicating that the starved cells were more sensitive to treatment with silver nanoparticles than vegetative cells. Surprisingly, the biofilm formation had not been inhibited by silver nanoparticles for both vegetative and starved cells. In conclusion, a new class of nanosilver containing disinfectant nanoproducts will be promising for advanced environmental treatments including air disinfection, water disinfection, surface disinfection and personal hygiene that will help to prevent the further outbreak of diseases.

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Controlling the Surface Plasmon Absorption of Silver Nanoparticles via Green Synthesis Using Pennisetum purpureum Leaf Extract

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.772.73 ◽

2018 ◽

Vol 772 ◽

pp. 73-77

Author(s):

Ruelson S. Solidum ◽

Arnold C. Alguno ◽

Rey Capangpangan

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Silver Nitrate ◽

Surface Plasmon ◽

Leaf Extract ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Absorption Peak ◽

Plasmon Absorption ◽

Surface Plasmon Absorption

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.

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The Synthesis and Stability Study of Silver Nanoparticles Prepared by Using p-Aminobenzoic Acid as Reducing and Stabilizing Agent

Indonesian Journal of Chemistry ◽

10.22146/ijc.29312 ◽

2018 ◽

Vol 18 (3) ◽

pp. 421 ◽

Cited By ~ 2

Author(s):

Dian Susanthy ◽

Sri Juari Santosa ◽

Eko Sri Kunarti

Keyword(s):

Silver Nanoparticles ◽

Reaction Time ◽

Silver Nitrate ◽

Tap Water ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Stability Study ◽

Synthesis Process ◽

Aminobenzoic Acid ◽

Stabilizing Agent

A study to examine the performance of p-aminobenzoic acid as both reducing agent for silver nitrate to silver nanoparticles (AgNPs) and stabilizing agent for the formed AgNPs has been done. The synthesis of AgNPs was performed by mixing silver nitrate solution as precursor with p-aminobenzoic acid solution and heating it in a boiling water bath. After the solution turned to yellow, the reaction stopped by cooling it in tap water. The formed AgNPs were analyzed by using UV-Vis spectrophotometry to evaluate their SPR absorption in wavelength range of 400–500 nm. The synthesis process was highly depend on the pH, reaction time, and mole ratios of the reactants. The synthesis only occur in pH 11 and at reaction time 30 min, the particle size of the formed AgNPs was 12 ± 7 nm. Longer reaction time increased the reducing performance of p-aminobenzoic acid in AgNPs synthesis but decreased its stabilizing performance. The increase of silver nitrate amount relative to p-aminobenzoic acid in the synthesis increased the reducing and stabilizing performance of p-aminobenzoic acid and the optimum mole ratio between AgNO3 and p-aminobenzoic acid was 5:100 (AgNO3 to p-aminobenzoic acid).

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Green Synthesis of Silver Nanoparticles Using Melia Azedarach and its Characterization, Corrosion and Antibacterial Properties

Biointerface Research in Applied Chemistry ◽

10.33263/briac111.85778586 ◽

2020 ◽

Vol 11 (1) ◽

pp. 8577-8586

Keyword(s):

Silver Nanoparticles ◽

Electron Microscope ◽

Color Change ◽

Nitrate Solution ◽

Antibacterial Properties ◽

Nanoparticle Synthesis ◽

Silver Nitrate Solution ◽

Xrd Analysis ◽

Melia Azedarach ◽

Change State

Nanoparticle synthesis by the biological method is economical and environmentally friendly. In the present study, the biosynthesis of silver nanoparticles is performed by using extracts of Melia Azedarach plant leaves. The synthesis is performed by adding to the silver nitrate solution to the leaf extract of Melia azedarach. The color change state the precipitation of nanoparticles of silver. The silver nanoparticles obtained were characterized by various techniques such as X-ray diffraction (XRD), Scanning electron microscope (SEM), transmission electron microscope (TEM), and Energy dispersive spectroscopy (EDX). The XRD analysis shows the particle size of 11 nm calculated by the Debye-Scherrer. The microstructure analysis shows silver particles of spherical and triangular. The corrosion rate was found to be 0.025 mpy. The green silver nanoparticle (AgNPs) synthesized have better antimicrobial potential against both bacteria’s (Bacillus subtilis and Pseudomonas aeruginosa).

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Pretreated Lignocellulosic Waste Mediated Biosynthesis of Silver Nanoparticles Under Room Temperature

International Journal of Nanoscience ◽

10.1142/s0219581x16600012 ◽

2016 ◽

Vol 15 (05n06) ◽

pp. 1660001 ◽

Cited By ~ 1

Author(s):

V. P. Manjamadha ◽

Karuppan Muthukumar

Keyword(s):

Silver Nanoparticles ◽

Silver Nitrate ◽

Room Temperature ◽

Nitrate Solution ◽

Silver Ions ◽

Silver Nitrate Solution ◽

Morphological Characterization ◽

Alternative Source ◽

X Ray ◽

Antibacterial Performance

The current work elucidates the utilization of biowaste as a valuable reducing agent for the synthesis of silver nanoparticles. In this study, the wastewater generated during the alkaline pretreatment of lignocellulosic wastes (APLW) was used as a bioreductant to reduce silver nitrate under room temperature. Synthesis of stable silver nanoparticles (AgNPs) was achieved rapidly on addition of APLW into the silver nitrate solution (1[Formula: see text]mM). The morphological characterization of AgNPs was performed using field emission scanning electron microscopy (FESEM). The micrograph clearly depicted the presence of spherical AgNPs. The presence of elemental silver along with biomoilties was determined using energy dispersive X-ray spectroscopy (EDAX) analysis. The X-ray diffraction (XRD) study proved the crystalline form of stable AgNPs. The AgNPs exhibited excellent antibacterial performance against Gram negative organism. The immediate bioreduction of silver ions using APLW was well illustrated in the present study. Thus, APLW serve as an alternative source for reducing agents instead of utilizing valuable medicinal plants for nanoparticles synthesis.

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Synthesis and Characterization of Silver Nanoparticles from Couroupita guianensis leaf extract–A Simple Green Route

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00493 ◽

2021 ◽

pp. 2796-2800

Author(s):

Inbathamizh L ◽

Kalpana V ◽

Revathi Yadav K

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Nitrate Solution ◽

Ultra Violet ◽

Silver Nitrate Solution ◽

Green Route ◽

Couroupita Guianensis ◽

Potential Applications ◽

Aqueous Leaf Extract

With the increase in the potential applications of nanoparticles in pharma and various fields, nanoparticle research is attracting more attention. Though several chemical and physical methods are being used for the synthesis of metal nanoparticles, they are associated with several disadvantages. Couroupita guianensis is a traditional plant with medicinal values. The focus of the study was to follow a green chemistry route to synthesize silver nanoparticles (AgNPs) using the leaf extract of Couroupita guianensis as a reductant and stabilizing agent. The boiled aqueous leaf extract with silver nitrate solution on exposure to sunlight showed the maximum absorbance at 430nm indicating the synthesis of AgNPs. Ultra Violet (UV)-Visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR) techniques were used for the characterization of AgNPs. The synthesized AgNPs were found to be spherical and 4.44 – 40.20nm in size. They also seemed to be capped with the significant functional groups present in the leaf extract. Thus, the study suggested Couroupita guianensis mediated green synthesis of AgNPs as an efficient and eco-friendly approach with substantial advantages over the conventional methods. The process could be further scaled-up for mass production and wider applications of AgNPs.

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