scholarly journals Photocatalytic Reduction of Methyl Orange, Antibacterial and Antibreast Cancer Activities of Biogenic Silver Nanoparticle Synthesized from Beta vulgaris Extract

2021 ◽  
Vol 33 (12) ◽  
pp. 2993-2998
Author(s):  
F. Femina ◽  
H. Asia Thabassoom ◽  
J. Felicita Florence
Keyword(s):  
Silver Nanoparticles ◽  
Methyl Orange ◽  
Beta Vulgaris ◽  
Anticancer Agents ◽  
Reduction Process ◽  
Cost Effective ◽  
Xrd Analysis ◽  
Photocatalytic Reduction ◽  
Hazardous Chemical ◽  
Average Size

Studies based on a biogenic synthesis of noble metal nanomaterials has become a promising one in today’s biomedical approach in therapy and diagnosis due to multidimensional applications. Hence, the present study is to explore the antibacterial, antibreast cancer and photocatalytic efficacy of silver nanoparticles (AgNPs) synthesized from an extract of Beta vulgaris (BV). The band at 398 nm in the UV-visible spectra confirmed the formation of AgNPs. The characteristic shift in OH and C=O peak after the formation of silver nanoparticles shows the participation of Beta vulgaris extracts in the reduction process which is further supported by from SEM morphology. The average size of the particle (17 nm) was determined from XRD analysis using Scherrer’s equation. Antibacterial results of Beta vulgaris mediated BV-AgNPs show the maximum zone of inhibition against Candida albicans. On anticancer activity, BV-AgNPs reveals the toxicity effect on the MCF-7 cell line with an IC50 value of 40.65 μg/mL. Similarly, it reduces 81.3% of methyl orange at 180 min on the photocatalytic reduction process. This study has suggested an effective replacement for the hazardous chemical methods and leads to a cost-effective, environmentally-friendly method that can also be used as antibacterial and anticancer agents.

scholarly journals Protocol optimization for a fast, simple and economical chemical reduction synthesis of antimicrobial silver nanoparticles in non-specialized facilities

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Roberto Vazquez-Muñoz ◽  
M. Josefina Arellano-Jimenez ◽  
Fernando D. Lopez ◽  
Jose L. Lopez-Ribot
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Reduction Process ◽  
Cost Effective ◽  
Silver Nitrate Solution ◽  
Synthesis Time ◽  
Average Size

Abstract Objective Silver nanoparticles (AgNPs) can be difficult or expensive to obtain or synthesize for laboratories in resource-limited facilities. The purpose of this work was to optimize a synthesis method for a fast, facile, and cost-effective synthesis of AgNPs with antimicrobial activity, which can be readily implemented in non-specialized facilities and laboratories. Results The optimized method uses a rather simple and rapid chemical reduction process that involves the addition of a polyvinylpyrrolidone solution to a warmed silver nitrate solution under constant vigorous stirring, immediately followed by the addition of sodium borohydride. The total synthesis time is less than 15 min. The obtained AgNPs exhibit an aspect ratio close to 1, with an average size of 6.18 ± 5 nm. AgNPs displayed potent antimicrobial activity, with Minimal Inhibitory Concentration values of ≤ 4 µg mL−1 for Staphylococcus aureus and ≤ 2 µg mL−1 for Candida albicans. The resulting method is robust and highly reproducible, as demonstrated by the characterization of AgNPs from different rounds of syntheses and their antimicrobial activity.

scholarly journals A fast and simple protocol for synthesizing effective antimicrobial silver nanoparticles in non-specialized facilities

2019 ◽  
Author(s):  
Roberto Vazquez-Munoz ◽  
M. Josefina Arellano-Jimenez ◽  
Jose L. Lopez-Ribot
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Chemical Reduction ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Antimicrobial Properties ◽  
Reduction Process ◽  
Cost Effective ◽  
Silver Nitrate Solution ◽  
Average Size

Abstract Objective Silver nanoparticles (AgNPs) can be difficult or expensive to obtain or synthesize for laboratories in resource-limited facilities. The purpose of this work was to create a fast, facile, and cost-effective method for synthesizing AgNPs with potent antimicrobial properties, that can be readily implemented in non-specialized laboratories.Results Our developed method uses a rather simple and rapid chemical reduction process that involves the addition of a polyvinylpyrrolidone solution to a warmed silver nitrate solution under constant vigorous stirring, immediately followed by the addition of sodium borohydride with constant stirring for an additional 15 minutes. AgNPs had an aspect ratio close to 1, with an average size of 6.18 ± 5 nm. AgNPs displayed potent antimicrobial activity, with Minimal Inhibitory Concentration values of 3 µg mL-1 and 1.5 µg mL-1 for Staphylococcus aureus and Candida albicans respectively.Keywords : Silver nanoparticles, nanoantibiotics, synthesis method, AgNPs, metallic nanoparticles

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals Green Synthesis of Silver Nanoparticles and Their Bactericidal and Antimycotic Activities against Oral Microbes

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Osvelia E. Rodríguez-Luis ◽  
Rene Hernandez-Delgadillo ◽  
Rosa Isela Sánchez-Nájera ◽  
Gabriel Alejandro Martínez-Castañón ◽  
Nereyda Niño-Martínez ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Fungal Growth ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Glycyrrhiza Glabra ◽  
X Ray Diffraction ◽  
Synthesize Silver Nanoparticles ◽  
Oral Biofilms ◽  
Oral Microbes ◽  
Average Size

Nanotechnology is a new discipline with huge applications including medicine and pharmacology industries. Although several methods and reducing agents have been employed to synthesize silver nanoparticles, reactive chemicals promote toxicity and nondesired effects on the human and biological systems. The objective of this work was to synthesize silver nanoparticles fromGlycyrrhiza glabraandAmphipterygium adstringensextracts and determine their bactericidal and antimycotic activities againstEnterococcus faecalisandCandida albicansgrowth, respectively. 1 and 10 mM silver nitrate were mixed with an extract ofGlycyrrhiza glabraandAmphipterygium adstringens.Green silver nanoparticles (AgNPs) were characterized by TEM, Vis-NIR, FTIR, fluorescence, DLS, TGA, and X-ray diffraction (XRD) analysis. Bactericidal and antimycotic activities of AgNPs were determined by Kirby and Bauer method and cell viability MTT assays. AgNPs showed a spherical shape and average size of 9 nm if prepared withGlycyrrhiza glabraextract and 3 nm if prepared withAmphipterygium adstringensextract. AgNPs inhibited the bacterial and fungal growth as was expected, without a significant cytotoxic effect on human epithelial cells. Altogether, these results strongly suggest that AgNPs could be an interesting option to control oral biofilms.

scholarly journals Bio Synthesis of Beta Vulgaris Mediated ZnO Nanoparticles and Their Anti-Bacterial, Anti-Fungal, Anti-Diabetic and Anti-Cancer Activities

2021 ◽  
Vol 37 (4) ◽  
pp. 911-921
Author(s):  
Femina. F ◽  
H. Asia Thabassoom ◽  
S. Auxilia Ruby ◽  
J. Felicita Florence
Keyword(s):  
Beta Vulgaris ◽  
Metal Oxide Nanoparticles ◽  
Cost Effective ◽  
Inhibitory Effect ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Hazardous Chemical ◽  
Vis Spectroscopy ◽  
Anti Cancer ◽  
Anti Fungal

Plant extract mediated metal or metal oxide nanoparticles is a promising alternate to the chemical and physical methods. Application of nanoparticles in various fields get increased widely due to their properties. Current study involves the eco-friendly method of synthesis and pharmacological applications of zinc oxide nanoparticles (ZnO-NPs) using the aqueous beetroot (Beta vulgaris) extract. Aqueous Beta vulgaris extract has multiple bio-active phytochemicals especially antioxidants like anthocyanins and betacyanins, also reported a rich source of minerals. Synthesized ZnONPs were characterized with the aid of UV-Vis spectroscopy, FTIR, SEM, XRDand EDX. Antibacterial results suggests that, Beta vulgaris mediated ZnONPspossessthe maximum zone of inhibition against Escherichia coli then Staphylococcus aureus. On anti-fungal activity synthesized ZnONPS revealed greater inhibitory effect against Candida albicans fungi than the Aspergillusflavus. Synthesized ZnONPs exhibits the noticeable pharmacological activities against the anti-diabetic and anti-cancer activities (MCF-7 cell line, IC50=35.4µg/ml). This study has suggested an effective replacement for the hazardous chemical methods and lead to a cost-effective, environmentally -friendly method which can also be used as an antimicrobial, anti-diabetic and anti-cancer agents.

scholarly journals Multifaceted Phytogenic Silver Nanoparticles by a Rare Insectivorous Plant Drosera Spatulata Labill Var.bakoensis and its Enhanced Antioxidant, Antibacterial, Antifungal and in Vitro Cytotoxic Studies

2021 ◽  
Author(s):  
Susmila Aparna Gaddam ◽  
Venkata Subbaiah Kotakadi ◽  
Gunasekhar. Kalavakunta ◽  
Josthna Penchalaneni ◽  
Varadarajulu Naidu Challagundla ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Radical Scavenging ◽  
Human Colon ◽  
Xrd Analysis ◽  
Antimicrobial Activities ◽  
Human Colon Cancer ◽  
Tem Analysis ◽  
Insectivorous Plant ◽  
Average Size

Abstract The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var.bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of their high negative zeta potential (-34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.

scholarly journals Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 855 ◽  
Author(s):  
Zubair Ahmed Ratan ◽  
Mohammad Faisal Haidere ◽  
Md. Nurunnabi ◽  
Sadi Md. Shahriar ◽  
A.J. Saleh Ahammad ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Agents ◽  
Recent Progress ◽  
Silver Ions ◽  
Cost Effective ◽  
Disease Diagnosis ◽  
Organic Nanoparticles ◽  
Anticancer Effects ◽  
Living Organisms ◽  
Bacteria And Fungi

Nanobiotechnology has grown rapidly and become an integral part of modern disease diagnosis and treatment. Biosynthesized silver nanoparticles (AgNPs) are a class of eco-friendly, cost-effective and biocompatible agents that have attracted attention for their possible biomedical and bioengineering applications. Like many other inorganic and organic nanoparticles, such as AuNPs, iron oxide and quantum dots, AgNPs have also been widely studied as components of advanced anticancer agents in order to better manage cancer in the clinic. AgNPs are typically produced by the action of reducing reagents on silver ions. In addition to numerous laboratory-based methods for reduction of silver ions, living organisms and natural products can be effective and superior source for synthesis of AgNPs precursors. Currently, plants, bacteria and fungi can afford biogenic AgNPs precursors with diverse geometries and surface properties. In this review, we summarized the recent progress and achievements in biogenic AgNPs synthesis and their potential uses as anticancer agents.

scholarly journals Green Synthesis of Silver Nanoparticles Using Astragalus tribuloides Delile. Root Extract: Characterization, Antioxidant, Antibacterial, and Anti-Inflammatory Activities

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2383
Author(s):  
Majid Sharifi-Rad ◽  
Pawel Pohl ◽  
Francesco Epifano ◽  
José M. Álvarez-Suarez
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Total Phenolics ◽  
Cost Effective ◽  
Root Extract ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
High Efficient ◽  
Anti Inflammatory ◽  
Average Size

Today, the green synthesis of metal nanoparticles is a promising strategy in material science and nanotechnology. In this research, silver nanoparticles (AgNPs) were synthesized through the high-efficient, cost-effective green and facile process, using the Astragalus tribuloides Delile. root extract as a bioreduction and capping agent at room temperature. UV–Vis spectroscopy was applied for the investigation of the reaction proceedings. To characterize the greenly synthesized AgNPs, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and transmission electron microscopy (TEM) analyses were utilized. In addition, the total phenolics and flavonoids contents, antioxidant, antibacterial, and anti-inflammatory activities of the greenly synthesized AgNPs and the A. tribuloides root extract were evaluated. The results indicated that the AgNPs had spherical morphology and crystalline structure with the average size of 34.2 ± 8.0 nm. The total phenolics and flavonoids contents of the greenly synthesized AgNPs were lower than those for the A. tribuloides root extract. The resultant AgNPs exhibited the appropriate antioxidant activity (64%) as compared to that for the A. tribuloides root extract (47%). The antibacterial test approved the higher bactericidal activity of the resulting AgNPs on the Gram-positive and Gram-negative bacteria in comparison to the A. tribuloides root extract. Considering the anti-inflammatory activity, the greenly synthesized AgNPs showed a stranger effect than the A. tribuloides root extract (82% versus 69% at 500 μg/mL). Generally, the AgNPs that were fabricated by using the A. tribuloides root extract had appropriate antioxidant, antibacterial, and anti-inflammatory activities and, therefore, can be considered as a promising candidate for various biomedical applications.

Synthesis and Characterization of Sol–Gel Prepared Silver Nanoparticles

2014 ◽  
Vol 13 (01) ◽  
pp. 1450004 ◽  
Author(s):  
Dharamvir Singh Ahlawat ◽  
Rekha Kumari ◽  
Rachna ◽  
Indu Yadav
Keyword(s):  
Silver Nanoparticles ◽  
Absorption Spectra ◽  
Annealing Temperature ◽  
Sol Gel ◽  
Xrd Analysis ◽  
Silica Matrix ◽  
Silver Clusters ◽  
Different Temperatures ◽  
Uv Visible ◽  
Average Size

Silver nanoparticles (SNPs) have been successfully prepared using sol–gel method by annealing the sample at 550°C for 30 min. The SNPs were not confirmed by X-ray diffraction (XRD) analysis when the annealing temperature was considered at 450°C. They were also not confirmed without calcination of the sample. The physical mechanism of silver clusters formation in the densified silica matrix with respect to thermal treatment has been understood. The presence of silver metal in the silica matrix was confirmed by XRD analysis and TEM image of the samples. The average size of nanoparticles dispersed in silica matrix was determined as 10.2 nm by the XRD technique. The synthesized nanocomposites were also characterized by UV-Visible spectroscopy with a peak in the absorption spectra at around 375 nm. The distribution of particle size has been reported here in the range from 8 nm to 25 nm by TEM observations of the sample prepared at 550°C. The spherically smaller size (≈10 nm) SNPs have reported the surface plasmons resonance (SPR) peak less than or near to 400 nm due to blue-shifting and effect of local refractive index. Without annealing the silica samples the absorption spectra does not show any peak around 375 nm. The FTIR spectroscopy of the three types of samples prepared at different temperatures (room temperature, 450°C and 550°C) has also been reported. This spectra have provided the identification of different chemical groups in the prepared samples. It has been predicted that the size of SNPs by XRD, UV-Visible and TEM results have agreed well with each other. It may be concluded that formation of SNPs is a function of annealing temperature.

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