scholarly journals Antibiotic susceptibility and genetic relatedness of Shigella species isolated from food and human stool samples in Qazvin, Iran

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Babak Pakbin ◽  
Abdollah Didban ◽  
Yousef Khazaye Monfared ◽  
Razzagh Mahmoudi ◽  
Amir Peymani ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Cross Sectional Study ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Sectional Study ◽  
Cross Sectional ◽  
Shigella Species ◽  
Rapd Pcr ◽  
Stool Samples ◽  
Human Stool

Abstract Objective The aim of this study was to investigate the genetic relatedness and antimicrobial resistance among Shigella species isolated from food and stool samples. Using cross sectional study method, Shigella spp. were isolated from food and clinical samples using culture-based, biochemical and serological methods. Antimicrobial susceptibility and genetic relatedness among the isolates were evaluated using disk diffusion and RAPD-PCR methods respectively. Results The prevalence of Shigella spp. were 4.84 and 7.7% in food and stool samples respectively. All food isolates were Sh. sonnei. 91.42% of the Shigella stool isolates were Sh. sonnei. 62.5% of food isolates were resistant to tetracycline. 46.8, 50 and 65.8% of clinical isolates were resistant to imipenem, amikacin and azithromycin respectively. 50 and 85.7% of the food and clinical isolates respectively were MDR. Dendrogram generated by RAPD-PCR showed that the isolates from food and stool samples were categorized in a same group. Close genetic relatedness between MDR Shigella isolates from food and clinical samples indicate that foods can be considered as one of the main vehicles for transmission of MDR Shigella to human causing acute diseases. Survey of MDR Shigella among food and clinical samples is strongly suggested to be implemented.

scholarly journals Prevalence of endoparasitors in rural communities in Central Brazil: a cross-sectional study

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
B Souza Rocha ◽  
R Alves Guimarães ◽  
P Sérgio Scalize ◽  
V Pagotto ◽  
F Souza Fiaccadori ◽  
...  
Keyword(s):  
Rural Communities ◽  
Rural Areas ◽  
Descriptive Analysis ◽  
Cross Sectional Study ◽  
Hymenolepis Diminuta ◽  
Clinical Samples ◽  
Sectional Study ◽  
Cross Sectional ◽  
Central Brazil ◽  
Stool Samples

Abstract Background Studies show that the magnitude is high in regions lacking basic sanitation, such as rural areas. Despite this, there is little evidence on the prevalence of endoparasitosis in rural communities in developing countries. The aim of this study was to estimate the prevalence of endoparasitosis in residents of rural communities in Central Brazil. Methods Cross-sectional study conducted within the scope of the Sanrural Project that aims to assess the health and sanitation conditions of rural communities in 2019. The target population of this study consisted of residents of 17 settlements, 24 quilombola and 7 riverside communities in Central Brazil. By listing the total number of residents of each household in the communities, residents were selected by simple random probability sampling. Then, stool samples were collected in sterile plastic containers and identified. After collection, the samples were transported for analysis by direct examination of the feces. The descriptive analysis was made. The study was approved by the Research Ethics Committee of the Federal University of Goiás. Results Data from 680 individuals (children and adults) who had stool samples collected were analyzed. The prevalence's of the endoparasites found were: Entamoeba coli (13.5%; 90%CI: 11.5-15.8%); Giardia lamblia (2.8%; 90%CI: 1.9-4.4); Ascaris lumbricoides (1.5%; 90%CI: 0.9-2.4); Ancyslotoma (0.9%; 90%CI: 0.5-1.7); Enterobius vermicularis (0.7%; 90%CI: 0.4-1.5%); Taenia (0.6%; 90%CI: 0.3-1.3); Hymenolepis nana (0.6%; 90%CI: 0.3-1,3); Itamoeba (0.4; 90%CI: 0.2-1.1); Hymenolepis diminuta (0.3%; 90%CI: 0.1-0.9), Strongiloides (0.0%); Trichuris (0.0%) and, Schistosoma mansoni (0.0%). Conclusions An intermediate prevalence for principal endoparasites was observed. This result may suggest the persistence of precarious sanitation conditions. Thus, the implications of the study include the need for systematic action by managers and health professionals in improving sanitation conditions. Key messages A current diagnosis will be made on the health and sanitation situation of rural communities in Goiás, based on the analysis of clinical samples and water quality, among other conditions. Based on this diagnosis, together with the community, we will seek solutions for sanitation and health through a Rural Sanitation Security Plan (PSSR).

Prevalence of Enterobacteriaceae spp. and its multidrug-resistant rates in clinical isolates: A two-center cross-sectional study

2021 ◽  
Author(s):  
Bahman Mirzaei ◽  
Ryhane Babaei ◽  
Zahra Norouzi Bazgir ◽  
Hamid Reza Goli ◽  
Shima Keshavarzi ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Clinical Isolates ◽  
Sectional Study ◽  
Cross Sectional

scholarly journals Gut Microbiome Composition Remains Stable in Individuals with Diabetes-Related Early to Late Stage Chronic Kidney Disease

Biomedicines ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 19
Author(s):  
Ashani Lecamwasam ◽  
Tiffanie M. Nelson ◽  
Leni Rivera ◽  
Elif I. Ekinci ◽  
Richard Saffery ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Relative Abundance ◽  
Cross Sectional Study ◽  
Early Event ◽  
Sectional Study ◽  
Cross Sectional ◽  
Microbiome Composition ◽  
Stool Samples ◽  
Late Stages

(1) Background: Individuals with diabetes and chronic kidney disease display gut dysbiosis when compared to healthy controls. However, it is unknown whether there is a change in dysbiosis across the stages of diabetic chronic kidney disease. We investigated a cross-sectional study of patients with early and late diabetes associated chronic kidney disease to identify possible microbial differences between these two groups and across each of the stages of diabetic chronic kidney disease. (2) Methods: This cross-sectional study recruited 95 adults. DNA extracted from collected stool samples were used for 16S rRNA sequencing to identify the bacterial community in the gut. (3) Results: The phylum Firmicutes was the most abundant and its mean relative abundance was similar in the early and late chronic kidney disease group, 45.99 ± 0.58% and 49.39 ± 0.55%, respectively. The mean relative abundance for family Bacteroidaceae, was also similar in the early and late group, 29.15 ± 2.02% and 29.16 ± 1.70%, respectively. The lower abundance of Prevotellaceae remained similar across both the early 3.87 ± 1.66% and late 3.36 ± 0.98% diabetic chronic kidney disease groups. (4) Conclusions: The data arising from our cohort of individuals with diabetes associated chronic kidney disease show a predominance of phyla Firmicutes and Bacteroidetes. The families Ruminococcaceae and Bacteroidaceae represent the highest abundance, while the beneficial Prevotellaceae family were reduced in abundance. The most interesting observation is that the relative abundance of these gut microbes does not change across the early and late stages of diabetic chronic kidney disease, suggesting that this is an early event in the development of diabetes associated chronic kidney disease. We hypothesise that the dysbiotic microbiome acquired during the early stages of diabetic chronic kidney disease remains relatively stable and is only one of many risk factors that influence progressive kidney dysfunction.

scholarly journals Identification of Hookworm Species in Stool By Harada Mori Culture

2012 ◽  
Vol 4 (2) ◽  
pp. 3-4 ◽  
Author(s):  
Sazzad Bin Shahid ◽  
A Chowdhury ◽  
S M Shamsuzzaman ◽  
K Z Mamun
Keyword(s):  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Laboratory Methods ◽  
Necator Americanus ◽  
Medical College ◽  
Stool Samples ◽  
Hookworm Infections

This cross sectional study was done from January 2009 to June 2010 in Microbiology department of Dhaka Medical College, Dhaka to identify hookworm species and to compare different laboratory methods for diagnosis of hookworm infections. Among the 375 stool samples evaluated, 26 (6.93%) samples were positive for hookworms. Ankylostoma duodenale were 3 (11.50%) and Necator americanus were 23 (88.50%). Harada Mori culture was found as the most effective method for detection of hookworms.DOI: http://dx.doi.org/10.3329/bjmm.v4i2.10821 

scholarly journals CHOLERA OUTBREAK IN WASIT GOVERNORATE, IRAQ 2017

2020 ◽  
Vol 2 (2) ◽  
pp. 211-219
Keyword(s):  
Acute Diarrhoea ◽  
Cross Sectional Study ◽  
Epidemiological Surveillance ◽  
Sectional Study ◽  
Cholera Outbreak ◽  
Cross Sectional ◽  
Endemic Diseases ◽  
Health Directorate ◽  
Stool Samples ◽  
Program Version

Introduction: Cholera is a major public health ongoing problem, causing significant morbidity and mortality particularly in developing countries. The main objective of the current study was to assess the distribution of cholera in Wasit governorate during the 2017 epidemic. Methods: This study was designed as a descriptive cross-sectional study. All patients were registered in health directorate records. The data collection lasted from 2nd of January 2018 till 28th February 2018 by formal registration for records of the directorate of Wasit health\ section of communicable diseases\ epidemiological surveillance unit. All registered cholera confirmed cases were included and frequency tables were done using SPSS program version 23. Results: The results showed that only (25) positive cases of cholera from (6175) total of stool samples taken from (20954) of acute diarrhoea recorded during 2017, and the highest percentage of cases (66.7%) were registered in September. The highest percentage of cases (32%) appear within the age category (>45) years while the lowest percentage was in under five years children(8%). Women constituted about (68%) of all cases. All cases were in Al-Suwaira health care district and all cases were of the type Inaba. All patients were recovered with no fatality. Even, the highest attack rate of cholera nationally and in Wasit was in 2015 outbreak. Conclusion: Cholera still one of the endemic diseases in Iraq that caused an outbreak at different times. Although, it still under control and need further work for special situations where the infection most likely distributed.

Immunological and molecular detection of rotavirus genotype in calves with gastroenteritis in Diyala-Iraq

2021 ◽  
Vol 1 (1) ◽  
pp. 001-013
Author(s):  
Ammar Talib Nasser ◽  
Abdulrazak Shafiq Hasan ◽  
Amer Khazaal Saleh ◽  
Mohammad Kassem Saleh
Keyword(s):  
Molecular Detection ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Rt Pcr ◽  
Cross Sectional ◽  
Reverse Transcription Pcr ◽  
Group A ◽  
Stool Antigen ◽  
Stool Samples ◽  
Age Range

Aim: To explore the prevalence of rotavirus infection along with the molecular detection and genotyping of group A rotavirus (RVA) among bovine calves up to 5 months old in Diyala province-Iraq. Methods: This is a cross sectional study conducted in Diyala province-Iraq during the period of 2019-2020. One hundred bovine calves with age range of 1-5 months were included in the study. All were suffering acute gastroenteritis. Serum anti-rotavirus IgM and IgG plus fecal rotavirus Ag were tested for using ELISA techniques. Stool samples positive for rotavirus Ag were submitted for reverse transcription PCR (RT-PCR) for G and P genes, followed by sequencing and genotyping thereafter. Statistical analysis was done using SPSS version 25 and P values ≤ 0.05 were considered significant. Results: The positivity rate of anti-rotavirus IgM was 80% (P = 0.0001), and that of anti-rotavirus IgG was 79% (P = 0.0001). The rotavirus stool antigen was detected in 68% of calves (P = 0.01). A total of 45 stool samples which were positive for rotavirus Ag were submitted for RT-PCR; 13 (28.9%) were positive and 32 (71.1%) were negative (P = 0.084). 10 PCR positive samples were used for sequencing and genotyping and indicated that all investigated strains belonged to G1P[8] genotype. Conclusion: The current strains analyzed belonged to the G1P[8] RVA genotypes, affirming that employment of VP7 gene polymorphism accurately yielded uniform phylogenetic distances amongst investigated rotavirus strains and that there were no noticeable assortment events between human and animal rotavirus strains in Diyala province.

scholarly journals Gambaran Infeksi Soil Transmitted Helminth Pada Petani Di Desa Gelgel Kabupaten Klungkung

2020 ◽  
Vol 4 (2) ◽  
pp. 21-30
Author(s):  
Putu indah Budi apsari ◽  
Ni Wayan Winianti ◽  
Heny Arwati ◽  
Yoes Prijatna Dachlan
Keyword(s):  
Informed Consent ◽  
Mixed Infection ◽  
Helminth Infection ◽  
Cross Sectional Study ◽  
Single Infection ◽  
Moist Soil ◽  
Sectional Study ◽  
Intensity Of Infection ◽  
Cross Sectional ◽  
Stool Samples

Soil transmitted helminth (STH) infections is kind of helminth infection which transmitted by contact with warm and moist soil especially affect the farmer. The purpose of this study was to investigate the profile of STH infection and the intensity of STH infection among farmers in Gelgel Village, Klungkung, Bali. The cross sectional study was conducted in Gelgel Village, Klungkung District, Bali and obtained stool and quitionaires from farmers by informed consent. Kato-Katz technique was used to diagnose the STH infection and to determine the intensity of infection based on the number of eggs per gram feces (EPG). Total 162 stool samples were collected from farmers aged 25-80 years, 22 farmers was positive of STH infection and 140 farmers was negative of STH infection. The result showed prevalence of STH infection was 13,5%, single infection of A. lumbricoides was 1.85%, 9.26% of T. trichiura single infection, and 0.61% of Hookworm single infection. The mixed infection was detected that were 1.23% of  A. lumbricoides with T. trichiura, and 0.61% of A. lumbricoides  with Hookworm. STH infection is the health problem among farmer. The farmer must be admitted antihelminthic drugs for preventing and eleminating STH infection.

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