scholarly journals Anti-diabetic effect of Stachytarpheta jamaicensis on low dose streptozotocin-induced diabetic rats fed on a high-fat diet

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mac Donald Idu ◽  
Owen – Obaseki Edehia ◽  
Benjamin Ogunma Gabriel
Keyword(s):  
High Fat Diet ◽  
Blood Glucose Concentration ◽  
Ethanol Extract ◽  
Scientific Basis ◽  
Negative Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
High Fat ◽  
Ameliorative Effect ◽  
Different Doses

Abstract Introduction This study evaluates the anti-diabetic effect of ethanol extract of Stachytarpheta jamaicensis leaf on streptozotocin (STZ) - induced diabetic rats fed on high-fat diet (HFD). Methods Sets of male albino rats of the Wistar strain weighing between 180 and 250 g were exposed to high fat diet (margarine and oil from vegetable sources in a ratio of 2:1 w/v) for 3 weeks. Then the animals were fasted overnight; hyperglycemic state was induced using reduced dose of streptozotocin (35 mg/kg) and animals were randomly divided into five groups (n = 7); group A received the HFD + STZ (35 mg/kg i.p.); group B received HFD + STZ + gliberclamide (10 mg/kg; i.p); groups C, D and E were administered the HFD + streptozotocin with different doses of the ethanol extract (30, 35 and 100 mg/kg p.o., respectively). Results Results showed significant (p < 0.05) decrease in blood glucose concentration of the rats treated with different doses of S. jamaicensis extract and those treated with gliberclamide compared to the untreated diabetic rats (negative control). Significant (p < 0.05) reductions in activities of serum AST, ALP, total protein and bilirubin were noticed in the groups in contrast to the control. Levels of urea, creatinine, potassium and chloride were considerably (p < 0.05) low while sodium and bicarbonate levels were high in the groups except the control. Lipid profile revealed significant (p < 0.05) reduction in total cholesterol, triacylglycerol, LDL, VLDL while HDL levels were high in the groups compared to the control. The extract significantly (p < 0.05) ameliorated weight loss. Histopathology of the liver, kidney and pancreas showed ameliorative effect of the extract against the deleterious changes occasioned by the HFD and STZ induced diabetic state. Conclusion These findings have provided scientific basis for the use of S. jamaicensis in the treatment of diabetes mellitus in ethnomedicinal practices in Nigeria.

scholarly journals Biochemical and Oxidative Changes in High Fat Diet/Streptozotocin-induced Diabetic Rats Treated with Metformin and the Polyherbal Diawell

2019 ◽  
pp. 1-11
Author(s):  
O. N. Briggs ◽  
E. O. Nwachuku ◽  
D. Tamuno-Emine ◽  
N. Nsirim ◽  
K. N. Elechi-Amadi
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
High Fat Diet ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
High Fat ◽  
Treatment Groups ◽  
Significant Difference ◽  
Oxidant Status

Diabetes mellitus is an epidemic, with a huge disease burden on the patients. This has led to an increase in the use of herbal remedies and combination therapies to reduce this burden. Aim: This study evaluates the biochemical and oxidative changes in type 2 diabetic rats, treated with metformin and the polyherbal drug diawell. Methodology: A total of 35 male Wistar albino rats weighing between 120-220 g were used for this study. The rats were placed on high fat diet, and diabetes was induced by a single intraperitoneal injection of freshly prepared streptozotocin (STZ) (45 mg/kg body wt). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Fasting plasma insulin (FPI), total oxidant status (TOS), total antioxidant status (TAS) and superoxide dismutase (SOD) levels were quantitatively determined by a rat-specific sandwich-enzyme linked immunosorbent assay (ELISA) method. Insulin resistance (IR) was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) method. Oxidative stress index (OSI) was determined by the ratio of TOS to TAS. Phytochemical analysis was also done on the herbal tablet. Results: Mean FPG levels were significantly lower (p˂0.05) in all groups, except the group administered diawell, which was not significantly different (p>0.05), compared to the diabetic control. Mean FPG levels were significantly higher (p˂0.05) in the metformin group, diawell group, but showed no significant difference (p>0.05) in the combination group, compared to the negative control. HOMA-IR was significantly higher (p<0.05) in the diabetic control compared to the negative control and treatment groups. The metformin and diawell groups had significantly higher (p˂0.05) HOMA-IR values, whereas the combination (metformin + diawell) showed no significant difference (p>0.05) when compared to the negative control. TOS was significantly higher (p<0.05) in the diabetic control compared to the negative control and treatment groups. The metformin and diawell groups had significantly higher (p˂0.05) TOS values, whereas the combination (metformin + diawell) showed no significant difference (p>0.05) when compared to the negative control. There was significantly lower (p˂0.05) TAS levels in the diabetic and treatment groups, compared to the negative control. OSI values were significantly lower (p˂0.05) in all groups when compared to the diabetic control. Also, OSI values were significantly higher (p˂0.05) in the treatment groups compared to the negative control. Conclusion: There was depletion of antioxidant parameters and an increase in oxidative stress in the diabetic rats. Administration of metformin and the polyherbal tablet diawell individually, were not effective in correcting the pathological and biochemical changes associated with diabetes. However, the combination treatment produced a better glycaemic response and attenuated the oxidant status in the rats. Antioxidant therapy should be incorporated in diabetes management, and anti-diabetic herbals properly evaluated.

Ameliorative effect of berberine on endothelial dysfunction in diabetic rats induced by high-fat diet and streptozotocin

2009 ◽  
Vol 620 (1-3) ◽  
pp. 131-137 ◽  
Author(s):  
Chunmei Wang ◽  
Jing Li ◽  
Xiaoyan Lv ◽  
Ming Zhang ◽  
Yanfang Song ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
High Fat Diet ◽  
Diabetic Rats ◽  
High Fat ◽  
Ameliorative Effect

scholarly journals Antihyperlidemic Efficiency of Abelmoschus esculentus (Okra) Fruits Varieties on Rats Fed High-Fat Diet

2020 ◽  
pp. 40-52
Author(s):  
A. A. Nuhu ◽  
D. H. Mhya ◽  
M. K. Atiku ◽  
A. M. Wudil
Keyword(s):  
Lipid Profile ◽  
High Fat Diet ◽  
Active Agent ◽  
Chemical Compounds ◽  
Basal Diet ◽  
Negative Control ◽  
High Fat ◽  
Fruit Extract ◽  
Control Serum ◽  
Different Doses

Background: Okra fruits have been found to be efficient in managing hyperlipidemia. But, there are different varieties of Okra, and whether antihyperlipidemic efficiency varies with the varieties has not been reported. Aim of the Study: The aim of the study was to validate antihyperlipidemic efficiencies of some Okra fruits varieties on rats fed high-fat diet. Methods: The five varieties of okra fruit was each sliced, air dried and pulverized into powder then extracted with methanol (80%) using Soxhlet extractor and concentrated at 30∘C in a rotary evaporator then finally air dried. Qualitative and quantitative phytochemicals, and proximate analysis were conducted on the extracts. Hyperlipidemia was induce by feeding rats with high-fat diet for 35 days, followed by treating with two selected Okra fruit varieties (NHB-AI-B and Yar kolon)  which has the highest extract yield and phytochemicals for 21 days. Nine groups of five rats was used: groups 1-3 (hyperlipidemic rats received NHB-AI-B Okra fruit extract at different doses), group 4-6 (hyperlipidemic rats received Yar kolon Okra fruit extract at different doses), group 7 (positive control rats treated with 10mg/kg Atorvastatin), group 8 (normal control rats fed basal diet) and group 9 (negative control). Serum lipid profile were determined from each rat. Results: The study confirmed the presence of some important phytochemicals like saponins, tannins, phenolics, flavonoids, fibre etc but differs in concentrations among the varieties where Yar kolon variety emerge highest in almost all the chemical compounds. Rat fed high-fat diet for 35 days developed hyperlipidemia as evident by the elevated triglyceride, cholesterol, LDL-C, body weight and supressed HDL-C. When treated with extracts of NHB-AI-B and Yar kolon Okra fruits varieties, the altered lipid profile were significantly reversed toward normalcy with Yar kolon variety exerting the most efficient activity. Conclusion: The study showed the five Okra fruit varieties possess same chemical compounds but differs in concentrations among varieties. The extracts of Okra fruits varieties exert significant antihyperlipidemic effect but in varied degrees suggesting variations in their efficiency. This, therefore calls for further study to compare more Okra varieties to determine which one is the most potent and its active agent.

scholarly journals Lipidaemic and Hepatic Status of Type 2 Diabetic Rats Treated with the Polyherbal Capsule Glucoblock

2020 ◽  
pp. 6-15
Author(s):  
O. N. Briggs ◽  
K. N. Elechi-Amadi ◽  
F. C. Ezeiruaku ◽  
R. E. Teme
Keyword(s):  
Liver Enzyme ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
High Fat ◽  
Treatment Groups ◽  
Type 2 Diabetic

The scourge of diabetes has led to an increase in the use of complementary and alternative medicine. The lack of regulation and control leads to the indiscriminate use of these herbals, with potential risk to patients. Aim: This study evaluates the lipidaemic and hepatic status of type 2 diabetic rats treated with the polyherbal capsule glucoblock. Methodology: A total of 35 male Wistar albino rats weighing between 120-220 g were used for this study. The rats were placed on high fat diet and diabetes was induced by a single intraperitoneal injection of freshly prepared streptozotocin (STZ) (45 mg/kg body wt). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Total Cholesterol (TC), Triglyceride (TG) and High Density Lipoprotein Cholesterol (HDL-C) were determined using enzymatic methods. Low Density Lipoprotein Cholesterol (LDL-C) was calculated using the Friedewald’s equation. Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) were determined using Reitman-Frankel method, while alkaline phosphatase (ALP) was determined using the colorimetric phenolphthalein method. Liver sections were stained using haematoxylin and eosin (H&E) staining technique, and phytochemical analysis was also done on the herbal capsule. Results: The results show no significant differences in TC levels in all groups compared to the negative control. TG level was significantly higher in the diabetic control group when compared to the negative control. TG level in the singular treatment groups were significantly lower, but the combination group (glibenclamide + glucoblock) showed no significant difference compared to the diabetic control. The negative control had significantly higher HDL-C compared to the diabetic control and treatment groups. There were no significant differences in HDL-C levels in all the treatment groups, when compared to the diabetic control. The negative control had significantly lower LDL-C compared to the diabetic control and treatment groups. There were no significant differences in LDL-C levels in all the treatment groups, when compared to the diabetic control. ALT, AST and ALP levels were significantly higher in the diabetic control, but was significantly reduced to normal levels by the treatments. Liver sections of the negative control showed normal histoarchitecture. The diabetic control showed inflammation and fatty deposition. The treatment groups showed a nearly normal histoarchitecture, with fatty deposits. Conclusion: High fat diet in combination with a sub-diabetic dose of streptozotocin produced significant diabetes in the Wistar rats with dyslipidaemia and elevated liver enzyme levels. The anti-diabetic treatments, glibenclamide and glucoblock did not correct the dyslipidaema caused by diabetes. However, the treatments had equipotent hepatoprotective effect and restored liver enzyme levels to normal as well as improving liver histology.

scholarly journals Evaluation of Anti-Hyperglycaemic activity of Ethanolic Extract of Stems of Bryophyllum pinnatum against Type 2 Diabetic rats

2021 ◽  
Vol 12 (2) ◽  
pp. 309-313
Author(s):  
Pandian P ◽  
Vijay A ◽  
Kathiresan K
Keyword(s):  
Low Dose ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Vehicle Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Bryophyllum Pinnatum

To evaluate antidiabetic activity of stems of Bryophyllum pinnatum in Alloxan male Wistar rats cause diabetes. Thirty albino rats were divided into distinct five groups (n=6). By Intraperitoneal injection caused the onset of diabetes of Alloxan (120mg/Kg). vehicle control, negative control, 200, and 400mg/kg ethanolic extracts were given orally in 28 days as single administrations to the vehicle control, diabetic control, normal group, and test groups.  Glucose, (fasting and post prandial for 1st and 21st day) serum cholesterol, Triglyceride, HDL (High density lipoprotein), LDL (Low density lipoprotein) Serum creatinine and urea were estimated with blood tests. The student's t test was performed to evaluate the results. In diabetic rats, a low dose of ethanol extract 200mg/kg significantly decreased all biochemical markers of diabetes. A 400mg/kg of ethanol extract was given to diabetic rats and showed substantial decreases in blood glucose and biochemical parameters. Both ethanol high and low dose extracts show antidiabetic activity in Alloxan induced diabetes.

scholarly journals Effect of Ethanol Extract of the Fruiting Bodies of Pleurotus Ostreatus on Serum Lipid Profile and Atherogenic Indices of HS-HFD-STZ Induced Diabetic Rats

2021 ◽  
pp. 11-21
Author(s):  
P. N. Okoroh ◽  
Sam Onuoha ◽  
A. A. Uwakwe ◽  
C. Y. Ukegbu
Keyword(s):  
Pleurotus Ostreatus ◽  
High Fat Diet ◽  
Serum Lipid ◽  
Ethanol Extract ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Fruiting Bodies ◽  
High Fat ◽  
Atherogenic Indices ◽  
High Sucrose

The effect of ethanol extract of the fruiting bodies of Pleurotus ostreatus on serum lipid profile  and atherogenic indices of high sucrose high fat diet streptozotocin (HS-HFD-STZ) induced diabetic rats was determined by standard methods. All the groups were fed high sucrose-high fat diet except the normal group. The Metformin HCl and ethanol extract was given once daily by gavage to the reference and experimental groups respectively at doses of 150mg/Kg b.w., 50mg/Kg   b.w, 150mg/Kg b.w. and 300mg/Kg b.w. respectively while the normal control received saline solution.  The HDL-cholesterol level was higher than the value of the disease group after 6 weeks of administration of extract at 300mg/kg and after 9 weeks administration of extract at 150mg/kg. HDL cholesterol concentration increased by 22.2% after 6 weeks of extract administration at 300mg/kg and by 16.7% and 28.3% respectively after 9 weeks of extract administration at 150mg/kg and 50mg/kg respectively, indicating that the POE extract has the capacity to reduce cardiovascular diseases. At all the doses of extract administered for treatment at 3 weeks, 6 weeks and 9 weeks intervals, there was dose and time dependent lowering of LDL: HDL ratio even below the recommended risk limit of ≤ 2.5 compared to the test control with value above 2.5. After 3 weeks of treatment with extract at administration concentration level of 150mg/kg, atherogenic indices were lowered and extract levels of 150mg/kg and 300mg/kg reduced atherogenic index value after 6 weeks of administration while after 9 weeks of treatment, extract at 150mg/kg concentration reduced atherogenic indices. The atherogenic indices of the diabetic animals under treatment were dose-and time dependently reduced by POE treatment as observed in this study.These results suggest a possible use of the extracts in the management of hyperlipidemic conditions, hypertension and associated complications.

Effects of Maternal Flaxseed Supplementation on Female Offspring of Diabetic Rats in Serum Concentration of Glucose, Insulin, and Thyroid Hormones

2019 ◽  
Vol 89 (1-2) ◽  
pp. 45-54
Author(s):  
Akemi Suzuki ◽  
André Manoel Correia-Santos ◽  
Gabriela Câmara Vicente ◽  
Luiz Guillermo Coca Velarde ◽  
Gilson Teles Boaventura
Keyword(s):  
Thyroid Hormones ◽  
High Fat Diet ◽  
Female Offspring ◽  
Flaxseed Oil ◽  
Diabetic Rats ◽  
High Fat ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Flaxseed Flour ◽  
Maternal Consumption

Abstract. Objective: This study aimed to evaluate the effect of maternal consumption of flaxseed flour and oil on serum concentrations of glucose, insulin, and thyroid hormones of the adult female offspring of diabetic rats. Methods: Wistar rats were induced to diabetes by a high-fat diet (60%) and streptozotocin (35 mg/kg). Rats were mated and once pregnancy was confirmed, were divided into the following groups: Control Group (CG): casein-based diet; High-fat Group (HG): high-fat diet (49%); High-fat Flaxseed Group (HFG): high-fat diet supplemented with 25% flaxseed flour; High-fat Flaxseed Oil group (HOG): high-fat diet, where soya oil was replaced with flaxseed oil. After weaning, female pups (n = 6) from each group were separated, received a commercial rat diet and were sacrificed after 180 days. Serum insulin concentrations were determined by ELISA, the levels of triiodothyronine (T3), thyroxine (T4) and thyroid-stimulating hormone (TSH) were determined by chemiluminescence. Results: There was a significant reduction in body weight at weaning in HG (−31%), HFG (−33%) and HOG (44%) compared to CG (p = 0.002), which became similar by the end of 180 days. Blood glucose levels were reduced in HFG (−10%, p = 0.044) when compared to CG, and there was no significant difference between groups in relation to insulin, T3, T4, and TSH after 180 days. Conclusions: Maternal severe hyperglycemia during pregnancy and lactation resulted in a microsomal offspring. Maternal consumption of flaxseed reduces blood glucose levels in adult offspring without significant effects on insulin levels and thyroid hormones.

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