Influence of oviposition-inducing hormone on spawning and mortality in the endangered Panamanian golden frog (Atelopus zeteki)

Abstract Background With Panamanian golden frogs (Atelopus zeteki; PGFs) likely extirpated from the wild, ensuring long-term sustainability of captive populations is crucial in order to conserve this critically endangered species. Unfortunately, PGFs display a unique reproductive behavior involving a prolonged period of amplexus leading to challenges in their successful captive propagation. The Maryland Zoo in Baltimore has observed high levels of mortality during the breeding season and suboptimal reproductive success leading to the use of hormone stimulation to aid in reproduction and health management. Methods This project aimed to develop induced ovulation and health management protocols by (1) evaluating different doses of gonadotropin releasing hormone analogue (GnRHa), (2) comparing the efficacy of GnRHa and GnRHa + metoclopramide, (3) determining latency periods and the effects of pulsed hormone sequences; and (4) establish if mortality is impacted by hormone therapy. Female PGFs (n = 174) were given GnRHa either in various concentrations (Experiment 1) or combined with metoclopramide (Experiment 2), and oviposition success, latency, and mortality were measured as binary response variables. Results Overall, the use of exogenous hormones significantly decreased mortality when compared to the control data of natural egg-laying females. GnRHa doses of 0.05 μg/g body weight produced similar ovulation rates compared to higher doses, and the addition of metoclopramide did not increase oviposition success compared to GnRHa alone. Lastly, results indicate the majority of female PGFs will release eggs within 48 h following the initial pulse of hormones with a small percentage ovipositing after a second pulse. Conclusion Findings from this study will benefit captive management of PGFs by documenting the increased survival of females when given hormone stimulation and defining appropriate GnRHa doses and expected latency to spawning.

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THE IMPACT OF VETOM 20.76 BASED ON PREDATORY FUNGUS ARTHROBOTRYS OLIGOSPORA ON THE LEUCOCYTES IN THE GEESE BLOOD

Bulletin of NSAU (Novosibirsk State Agrarian University) ◽

10.31677/2072-6724-2019-53-4-103-108 ◽

2019 ◽

pp. 103-108

Author(s):

N. S. Iakovleva ◽

G. A. Nozdrin ◽

M. S. Iakovleva ◽

S. N. Tishkov ◽

A. I. Shevchenko

Keyword(s):

Live Weight ◽

Control Group ◽

Group 2 ◽

The Impact ◽

Higher Doses ◽

Leukocyte Concentration ◽

Experimental Group ◽

Different Doses ◽

Group 1

The paper demonstrates the results on the effect of new specimen Vetom 20.76 on concentration of leukocytes in the blood of geese on the basis of the predatory fungus Artusbotus oligospora. In order to achieve the goal of the experiment, one control group and six experimental groups were arranged on the principle of paired analogues. Each group contained 10 geese aged 1 month. The geese from the experimental groups received Vetom 20.76 in different doses in the morning with water once a day: the geese of the 1st experimental group - dose of 0.5 ppm/kg of live weight during 15 days; 2nd experimental group - 1 ppm/kg of live weight during 15 days; 3rd experimental group - 2 ppm/kg of live weight during 15 days, 4th experimental group - 0.5 ppm/kg live weight during 30 days, 5th experimental group - 1 ppm/kg live weight during 30 days and 6th - 2 ppm/kg live weight during 30 days. The geese of control group didn’t receive the specimen. The concentration of leukocytes in the blood of experimental geese increases in the period of specimen application as well as in the period of its aftereffect. If Vetom 20.76 is prescribed for 15 days, the effect of leukopoiesis stimulation finishes on the 30th day. If the specimen is applied during 30 days, the leukocytes in the blood continue to increase up to the 60th day. This long-term application of Vetom 20.76 dosed 0.5ppm/kg increases leucocytes within the physiological norm. Application of higher doses (1 and 2 ppm/kg) the leukocyte concentration conforms to the physiological norm

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Удобрение огурца в Западной Сибири

Kartofel` i ovoshi ◽

10.25630/pav.2018.55.12.003 ◽

2018 ◽

Author(s):

Т.А. Кuznetsova ◽

N.А. Коlpakov

Keyword(s):

Organic Fertilizers ◽

Control Variant ◽

Positive Effect ◽

Different Doses

Показано, что длительное применение как минеральных, так и органических удобрений в различных дозах и сочетаниях положительно влияет на урожайность огурца. Установлено, что в условиях 14-й ротации урожайность на всех вариантах опыта была выше, чем на контрольном варианте и составила 16,4–22,3 т/га, против 16,0 т/га в контроле.The authors show that the long-term use of both mineral and organic fertilizers in different doses and combinations has a positive effect on the yield of cucumber. It was found that under 14 rotation conditions the yield on all variants of the experiment was higher than on the control variant and amounted to 16.4–22.3 t/ha, against 16.0 t/ha on the control.

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The Effects of N-Acetylcysteine on the Rat Mesocorticolimbic Pathway: Role of mGluR5 Receptors and Interaction with Ethanol

Pharmaceuticals ◽

10.3390/ph14060593 ◽

2021 ◽

Vol 14 (6) ◽

pp. 593

Author(s):

Sandra Fernández-Rodríguez ◽

Claudia Esposito-Zapero ◽

Teodoro Zornoza ◽

Ana Polache ◽

Luis Granero ◽

...

Keyword(s):

Behavioral Activation ◽

Ethanol Administration ◽

Metabotropic Receptors ◽

Negative Allosteric Modulator ◽

High Doses ◽

Cell Expression ◽

Mucolytic Agent ◽

Different Doses

N-acetylcysteine (NAC) is a prodrug that is marketed as a mucolytic agent and used for the treatment of acetaminophen overdose. Over the last few decades, evidence has been gathered that suggests the potential use of NAC as a new pharmacotherapy for alcohol use disorder (AUD), although its mechanism of action is already being debated. In this paper, we set out to assess both the potential involvement of the glutamate metabotropic receptors (mGluR) in the possible dual effect of NAC administered at two different doses and NAC’s effect on ethanol-induced activation. To this aim, 30 or 120 mg/kg of NAC was intraperitoneally administered to rats with the presence or absence of the negative allosteric modulator of mGluR5 (MTEP 0.1 mg/kg). Thereafter, the cFOS IR-cell expression was analyzed. Secondly, we explored the effect of 120 mg/kg of NAC on the neurochemical and behavioral activation induced by intra-VTA ethanol administration (150 nmol). Our results showed that the high NAC dose stimulated cFOS expression in the NAcc, and that this effect was suppressed in the presence of MTEP, thus suggesting the implication of mGluR5. Additionally, high doses could attenuate the ethanol-induced increase in cFOS-expression in the NAcc, probably due to a phenomenon based on the long-term depression of the MSNs. Additional experiments are required to corroborate our hypothesis.

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Glycemic Control Potential of Chemically Characterized Extract from Withania frutescens L. Roots in Severe Diabetes-Induced Mice

Applied Sciences ◽

10.3390/app11093998 ◽

2021 ◽

Vol 11 (9) ◽

pp. 3998

Author(s):

Abdelfattah El Moussaoui ◽

Hamza Mechchate ◽

Mohammed Bourhia ◽

Imane Es-safi ◽

Ahmad Mohammad Salamatullah ◽

...

Keyword(s):

Chemical Analysis ◽

Plant Extract ◽

Biochemical Parameters ◽

Pharmaceutical Drugs ◽

Glucose Lowering ◽

Natural Agents ◽

Severe Diabetes ◽

Treatment Of Diabetes ◽

Different Doses

Diabetes mellitus is a metabolic syndrome that causes impairment, mortality, and many other complications. Insulin and several synthetic medications are currently used in the treatment of diabetes. However, these pharmaceutical drugs are costly, and therefore medicines place priority on alternatives to fight this lethal disease. This modest study aims to investigate the chemical composition, antidiabetic and antihyperglycemic potentials along with subacute toxicity (bodyweight change and biochemical parameters) of hydroethanol extract from Withania frutescens L. roots (WFRE). The chemical analysis was carried out using GC–MS after extract silylation. The chemical analysis identified many potentially active compounds that may determine the antidiabetic results of WFRE. The antidiabetic effect of WFRE was evaluated in mice with severe diabetes using oral administration of doses up to 400 mg/kg for 28 days. The results of the antidiabetic and antihyperglycemic tests indicate that WFRE possesses promising glucose-lowering effects and, as a result, it may serve as an antidiabetic alternative for long-term use. The 4-week treatments with different doses of plant extract did not alter the bodyweight appearance of the diabetic mice nor their biochemical parameters (AST and ALT). The findings obtained indicate that the studied plant extract controlled severe diabetes in mice. Therefore, Withania frutescens L. can serve society as it provides natural agents to control diabetes.

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Long-term effects of fine particulate matter exposure on the progression of arterial stiffness

Environmental Health ◽

10.1186/s12940-020-00688-6 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Dianqin Sun ◽

Yue Liu ◽

Jie Zhang ◽

Jia Liu ◽

Zhiyuan Wu ◽

...

Keyword(s):

Arterial Stiffness ◽

Health Management ◽

Ankle Brachial Index ◽

Population Data ◽

Average Concentration ◽

Air Pollutant ◽

Stiffness Index ◽

Long Term Effects ◽

Annual Average

Abstract Background Prior studies have investigated the association of PM2.5 exposure with arterial stiffness measured by ankle-brachial index (ABI) and brachial-ankle pulse wave velocity (baPWV), of which conclusions are inconsistent. Moreover, limited evidence is available on the contributory role of PM2.5 exposure on the arterial stiffness index. Methods We used the population data from the Beijing Health Management Cohort and conducted a longitudinal analysis. The annual average concentration of PM2.5 for 35 air pollutant monitoring sites in Beijing from 2014 to 2018 was used to estimate individual exposure by different interpolation methods. Multivariate logistic regression and linear regression were conducted to assess the association of annual average PM2.5 concentration with the incidence of higher baPWV, the progression of ABI, and baPWV, respectively. Results The association between PM2.5 exposure and incidence of higher baPWV was not significant (OR = 1.11, 95% CI: 0.82–1.50, P = 0.497). There was − 0.16% (95% CI: − 0.43-0.11%) decrease in ABI annually and 1.04% (95% CI: 0.72–1.37%) increase in baPWV annually with each increment of 10 μg/m3 average PM2.5 concentration. Conclusions Long-term exposure to PM2.5 was associated with the progression of arterial stiffness in Beijing. This study suggests that improvement of air quality may help to prevent arterial stiffness.

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Wearable Wireless Physiological Monitoring System Based on Multi-Sensor

Electronics ◽

10.3390/electronics10090986 ◽

2021 ◽

Vol 10 (9) ◽

pp. 986

Author(s):

Hongru Li ◽

Guiling Sun ◽

Yue Li ◽

Runzhuo Yang

Keyword(s):

Monitoring System ◽

Health Management ◽

Wearable Technology ◽

Physiological Monitoring ◽

Motion Data ◽

Low Load ◽

Measure Heart Rate ◽

Body Surface Temperature ◽

Continuous Work

The purpose of wearable technology is to use multimedia, sensors, and wireless communication to integrate specific technology into user clothes or accessories. With the help of various sensors, the physiological monitoring system can collect, process, and transmit physiological signals without causing damage. Wearable technology has been widely used in patient monitoring and people’s health management because of its low-load, mobile, and easy-to-use characteristics, and it supports long-term continuous work and can carry out wireless transmissions. In this paper, we established a Wi-Fi-based physiological monitoring system that can accurately measure heart rate, body surface temperature, and motion data and can quickly detect and alert the user about abnormal heart rates.

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Editorial

Journal of Health Management ◽

10.1177/097206340901100101 ◽

2009 ◽

Vol 11 (1) ◽

pp. 1-14 ◽

Cited By ~ 1

Author(s):

Manasi Kumar ◽

Erica Burman

Keyword(s):

Millennium Development Goals ◽

Health Management ◽

The Political ◽

Special Issue ◽

Wide Range ◽

Critical Approaches ◽

Development Goals ◽

Short And Long Term ◽

Primary Focus

We welcome readers to the first special issue (11.1) of the Journal of Health Management. We hope the readers find the articles and various reviews enriching and provocative, both in terms of the range of ideas and critical approaches addressed. The key theme of this double issue concerns the political limits of mega-development projects such as the Millennium Development Goals (MDGs). The primary focus of the articles collected here is to provide an insightful, constructive and in-depth critique of the United Nations (UN) MDGs along with critical deliberations on their short- and long-term implications not only for health management but also for a wide range of issues around development and social change.

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Effect of chronic intraperitoneal aminoguanidine on memory and expression of Bcl-2 family genes in diabetic rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2015-0357 ◽

2016 ◽

Vol 94 (6) ◽

pp. 669-675 ◽

Cited By ~ 4

Author(s):

Mohsen Alipour ◽

Fatemeh Adineh ◽

Hossein Mosatafavi ◽

Azam Aminabadi ◽

Hananeh Monirinasab ◽

...

Keyword(s):

Diabetic Rats ◽

Male Rats ◽

Cognitive Tasks ◽

Negative Effects ◽

Gene Expressions ◽

Family Gene ◽

Higher Doses ◽

With Memory ◽

Hippocampal Apoptosis

Long-term hyperglycemia associates with memory defects via hippocampal cells damaging. The aim of the present study was to examine the effect of 1 month of i.p. injections of AG on passive avoidance learning (PAL) and hippocampal apoptosis in rat. Eighty male rats were divided into 10 groups: control, nondiabetics and STZ-induced diabetics treated with AG (50, 100, 200, and 400 mg/kg, i.p.). PAL and the Bcl-2 family gene expressions were determined. Diabetes resulted in memory and Bcl-2 family gene expression deficits. AG (50 and 100 mg/kg) significantly improved the learning and Bcl-2, Bcl-xl, Bax, and Bak impairment in diabetic rats. However, negative effects were indicated by higher doses of the drug (200 and 400 mg/kg). Present study suggests that 1 month of i.p. injections of lower doses of AG, may improve the impaired cognitive tasks in STZ-induced diabetic rats possibly by modulating Bcl-2 family gene expressions.

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Intrapleural analgesia after endoscopic thoracic sympathectomy

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000600017 ◽

2011 ◽

Vol 26 (6) ◽

pp. 508-513 ◽

Cited By ~ 1

Author(s):

Patrícia Gomes da Silva ◽

Daniele Cristina Cataneo ◽

Fernanda Leite ◽

Erica Nishida Hasimoto ◽

Guilherme Antonio Moreira de Barros

Keyword(s):

Postoperative Period ◽

Inspiratory Capacity ◽

Intense Pain ◽

Thoracic Sympathectomy ◽

Group A ◽

Group B ◽

Intrapleural Analgesia ◽

Higher Doses ◽

Late Postoperative Period ◽

Different Doses

PURPOSE: To compare analgesia traditionally used for thoracic sympathectomy to intrapleural ropivacaine injection in two different doses. METHODS: Twenty-four patients were divided into three similar groups, and all of them received intravenous dipyrone. Group A received intravenous tramadol and intrapleural injection of saline solution. Group B received intrapleural injection of 0.33% ropivacaine, and Group C 0.5% ropivacaine. The following aspects were analyzed: inspiratory capacity, respiratory rate and pain. Pain was evaluated in the immediate postoperative period by means of the visual analog scale and over a one-week period. RESULTS: In Groups A and B, reduced inspiratory capacity was observed in the postoperative period. In the first postoperative 12 hours, only 12.5% of the patients in Groups B and C showed intense pain as compared to 25% in Group A. In the subsequent week, only one patient in Group A showed mild pain while the remainder reported intense pain. In Group B, half of the patients showed intense pain, and in Group C, only one presented intense pain. CONCLUSION: Intrapleural analgesia with ropivacaine resulted in less pain in the late postoperative period with better analgesic outcomes in higher doses, providing a better ventilatory pattern.

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Effect of Pericampylus glaucus on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i1.24593 ◽

2016 ◽

Vol 11 (1) ◽

pp. 200 ◽

Cited By ~ 1

Author(s):

Muhammad Kifayatullah ◽

Pinaki Sengupta

Keyword(s):

Lipid Profile ◽

Plasma Glucose ◽

Glucose Concentration ◽

Ethanolic Extract ◽

Diabetic Rats ◽

Plasma Glucose Concentration ◽

Short And Long Term ◽

Different Doses ◽

Long Term Studies

The purpose of this study was to evaluate the effects of Pericampylus glaucus extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats. The ethanolic extract were administered orally at three different doses (400, 600 and 800 mg/kg) and glibenclamide (20 mg/kg p.o.) for 21 days after 72 hours of streptozotocin injection. During the short- and long-term studies, the extract was found to possess significant (p<0.01, p<0.001) anti-diabetic activity in normal and diabetic rats compared with untreated normal and untreated diabetic group. It also caused reduction in the level of total cholesterol, triglyceride, and LDL etc. and improvement in the HDL level compared with untreated diabetic rats. Reduction in the fasting blood sugar, cholesterol, triglyceride, urea, LDL, creatinine levels and improvement in the HDL by P. glaucus indicates that plant has anti-diabetic activity along with anti hyperlipidemic efficacy and provides a scientific rationale for the use.

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