scholarly journals Development of Chrysomya albiceps (Wiedemann, 1819) (Diptera: Calliphoridae) from the Jazan region of Southwest Saudi Arabia under different laboratory temperatures: applications in forensic entomology

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hanan Abo El-Kassem Bosly
Keyword(s):  
Saudi Arabia ◽  
Forensic Entomology ◽  
Developmental Stages ◽  
Life Stage ◽  
Pupal Weight ◽  
Adult Longevity ◽  
Larval Body ◽  
Ambient Temperatures ◽  
Chrysomya Albiceps

Abstract Background Chrysomya albiceps (Wiedemann, 1819) (blowflies), family Calliphoridae, is important in forensic entomology, where the minimum and maximum postmortem intervals (PMI) are estimated on the basis of the developmental stages of Diptera larvae that consume dead tissue. The present study was designed to estimate the effects of different ambient temperatures (20, 25, and 30 °C) under controlled laboratory conditions on the developmental stages of C. albiceps from the Jazan region, Saudi Arabia. Results The present study showed that the larval body weight and length were significantly increased when larvae were reared at 30 °C compared with corresponding values at 24 h, 48 h, and 72 h at rearing temperatures of 20 °C and 25 °C; however, the weight and length were significantly decreased compared with corresponding values at 96 h at 20 °C and 25 °C. The pupation time was inversely related to the rearing temperature, occurring at 144, 124, and 120 h at rearing temperatures of 20 °C, 25 °C, and 30 °C, respectively. The pupal weight and length were significantly increased in larvae reared at 30 °C compared with those reared at 20 °C and 25 °C. At 20 °C, 25 °C, and 30 °C, larval durations of 5.00, 5.00, and 4.00 days were recorded, respectively. Pupae and adults showed gradual decreases in life stage durations, at 6.00, 5.30, and 4.80 days in pupae and 20.00, 18.70, and 16.90 days in adults, with increasing rearing temperatures. Average adult longevity at 30 °C (194.40 h) was significantly less than adult longevity at 20 °C (216.00 h) and 25 °C (204.60 h). The results showed an inverse relation between durations of developmental stages and rearing temperatures. Conclusions Insect laboratory colonization for the determination of biological characteristics of insects is economically viable for forensic entomology and as a technique for evaluating insect evidence.

scholarly journals Thermal summation model and instar determination of all developmental stages of necrophagous beetle,Sciodrepoides watsoni(Spence) (Coleoptera: Leiodidae: Cholevinae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1944 ◽  
Author(s):  
Pavel Jakubec
Keyword(s):  
Forensic Entomology ◽  
Developmental Stages ◽  
Larval Instar ◽  
Head Capsule ◽  
Common Species ◽  
Standard Errors ◽  
Future Studies ◽  
Post Mortem Interval ◽  
Holarctic Region

Necrophagous beetles are underrepresented in forensic entomology studies despite their undeniable utility for the field. In the present article, information is presented regarding the developmental biology and instar determination ofSciodrepoides watsoni(Spence, 1813), a very common species occurring across the Holarctic region. Wild collected beetles were kept in climate chambers at constant temperature (12, 15, 18, 21 and 28 °C) and their development was regularly documented. Parameters of thermal summation models and standard errors were calculated for each developmental stage. These models may be used for an estimation of post-mortem interval in legal investigations after further validation on local populations ofS. watsoni. An additional methodology is introduced for future studies of size-based characteristics, addressing instar identification bias. The methodology provided estimations (mean, standard error and standard deviation) ofS. watsonilarval head capsule width for preliminary larval instar determination. The methodology may be used with other morphological features to improve instar determination accuracy.

Recherches sur le rôle de la température dans la réalisation du phénotype chez des embryons de l'Amphibien Pleurodeles waltlii homozygotes pour la mutation thermosensible ac (ascite caudale)

Development ◽  
1975 ◽  
Vol 34 (1) ◽  
pp. 221-252
Author(s):  
Par Maria Fernandez ◽  
Jean-Claude Beetschen
Keyword(s):  
Embryonic Development ◽  
Developmental Stages ◽  
Fluid Collection ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Tail Bud ◽  
Dorsal Fin ◽  
Second Shift ◽  
Pleurodeles Waltlii

1. At the feeding stage (st. 38), a high percentage (79 %) of Pleurodeles homozygous ac/ac larvae show bent tails after a persistent ascitic blister in the dorsal part of the fin, when embryonic development occurred at 12°C; about only 25 % of them are affected by abdominal and pericardic ascites; about 40 % can feed and survive. The larval phenotype is very different when embryonic development occurred at 23 °C, in which case tail growth appears to be normal, but 95 % larvae die, due to ascitic fluid collection in the abdominal and heart regions, marked anaemia and microcephaly. 2. The exchange of posterior neural plates and dorso-lateral epidermis between normal and mutant neurulae has shown that the localization of the blister in the dorsal fin is not dependent on autonomous properties of the mutant dorsal tissues, but should be considered as resulting from general disturbances in the mutant organism. 3. Experiments were performed, involving a temperature shift from 12 to 23°C or 23 to 12°C, occurring at various developmental stages from the end of gastrulation (stage 13) to the stage of spontaneous embryonic muscle contractions (stage 26). When the temperature shift was applied after the end of neurulation (stage 21), the caudal phenotype was statistically similar to that of larvae which had been bred continuously at the first temperature. Thus temperature-sensitive phases can be characterized between neurula stages 15 and 18 (for a 12–23° shift) or 15 and 21 (for a 23–12° shift). Similarly, abdominal ascites can be induced when embryos are kept at 23 °C till stage 23 (early tail-bud) only, and occurs much less frequently when embryos are kept at 12°C till stage 23 and then transferred to 23°C. 4. It could be concluded from these experiments that the caudal mutant phenotype is already temperature-determined during neurulation, before stage 21. Nevertheless, double temperature-shift experiments showed that the second shift could modify the results which would be obtained if the first shift only occurred. Paradoxical results were obtained, more than 90 % of the tail phenotypes being of the ‘warm type’ when the embryos were first kept at 12°C, then shifted up to 23 °C between stages 22 and 26, and shifted down again to 12°C. Such a treatment markedly lowers the percentage of bent tails (‘cold type’) from the percentage which would occur if ac/ac embryos were constantly kept at 23 °C after stage 21, but this longer warm treatment is of no effect of itself as compared to the case when the whole development occurs at 12°C (bent tails are predominant in this latter case). Thus, whereas the early determination of the position of the caudal blister can be considered as a stable phenomenon under given temperature conditions, it is not irreversible. 5. As compared to cold-bred larvae, thrice as many completely anaemic larvae (66 %) were obtained from ac/ac embryos kept at 23 °C between stages 21 and 26; this offers an opportunity for the experimental study of this anaemia. 6. Implications of these results for further analysis of temperature-sensitive mutations in cold-blooded vertebrates are suggested.

Faunistic inventory and zoogeographical analysis of the ground beetles (Coleoptera, Carabidae) of Garf Raydah Nature Reserve, Southwestern of Saudi Arabia, and description of a new species of Paussinae

Zootaxa ◽  
2018 ◽  
Vol 4514 (3) ◽  
pp. 341 ◽  
Author(s):  
MAHMOUD S. ABDEL-DAYEM ◽  
IFTEKHAR RASOOL ◽  
ALI A. ELGHARBAWY ◽  
PETER NAGEL ◽  
HATHAL M. ALDHAFER
Keyword(s):  
Saudi Arabia ◽  
Life Form ◽  
Nature Reserve ◽  
Arabian Peninsula ◽  
New Records ◽  
Ground Beetles ◽  
Kingdom Of Saudi Arabia ◽  
Form Analysis ◽  
A New Species

Study of ground beetles of the Garf Raydah Nature Reserve, located in the Asir Mountains of southwestern Kingdom of Saudi Arabia (KSA) resulted in one species, Paussus abditus Nagel, sp. n. described as new to science. Thirteen species (21.3%) are reported as new country records and fifteen species (24.6%) are new records for Asir Province. Adult beetles were collected from 2013 to 2017. The determination of this material yielded a total of 61 species in 40 genera and 17 tribes belonging to nine subfamilies of Carabidae. The species richness represented approximately 36.1% of carabid species previously reported from KSA. The most species rich tribes were the Lebiini (20 species), the Harpalini (10 species), and the Bembidiini (6 species). The life form analysis of adults indicated 18 life form types that are grouped into three categories, Zoophagous (77.1%), Mixophytophagous (18.0%), and Myrmecophilous (4.9%). Zoogeographical analyses indicated that the Afrotropical (19.3%) and the Saharo-Arabian (19.3%) species dominate the carabid fauna of this region of KSA. Coryza cf. maculata (Nietner, 1856) is considered the only Oriental representative. Only one cosmopolitan species, Perigona nigriceps (Dejean, 1831), was collected. Eleven endemics were identified; six species are considered KSA endemics and five are Arabian Peninsula endemics. 

scholarly journals Heat unit accumulation and inflorescence and fruit development in ‘Ubá’ mango trees grown in Visconde do Rio Branco-MG

2018 ◽  
Vol 40 (2) ◽  
Author(s):  
Lorena Moreira Carvalho Lemos ◽  
Luiz Carlos Chamhum Salomão ◽  
Dalmo Lopes de Siqueira ◽  
Olinto Liparini Pereira ◽  
Paulo Roberto Cecon
Keyword(s):  
Fruit Development ◽  
Fruit Set ◽  
Developmental Stages ◽  
Mangifera Indica ◽  
Developmental Cycle ◽  
Flower Bud ◽  
Ambient Temperatures ◽  
Heat Units ◽  
Mango Tree ◽  
Apical Bud

Abstract There are little information in the scientific literature on flowering and fruiting of ‘Ubá’ mango trees. These information enables to know the proportion of hermaphrodite flowers in inflorescence, fruit set percentage and developmental stages of the fruit. In this study evaluations on inflorescence and fruit development of the ‘Ubá’ mango tree (Mangifera indica L.) were carried out, as well as the determination of the required number of heat units for full fruit development. Thirty branches whose terminal buds were swollen were selected from five mango trees. With the aid of a camera and a caliper, the panicle and fruit development were evaluated weekly until full fruit development. A digital thermometer was used to record ambient temperatures during fruit development in order to estimate the number of heat units required for complete development of the fruits. Male and hermaphrodite flowers of the panicles were also identified and counted. The developmental cycle of ‘Ubá’ mango from the beginning of apical bud swelling to commercial harvest of the fruit lasted 168 days in 2011 and 154 days in 2012. The number of hermaphrodite flowers and the percentage of fruit set in the inflorescence in 2011 were 32.3 and 0.066%, respectively; and 122.1 and 0.099% in 2012, respectively. There was accumulation of 3,173 heat units from flower bud swelling to full development of the ‘Ubá’ mangoes.

A Model for Droplet Vaporization for Use in Gasoline and HCCI Engine Applications

2004 ◽  
Vol 126 (2) ◽  
pp. 422-428 ◽  
Author(s):  
Youngchul Ra ◽  
Rolf D. Feitz
Keyword(s):  
Heat Flux ◽  
Internal Heat ◽  
Droplet Surface ◽  
Fuel Vapor ◽  
Gas Mixture ◽  
Ambient Temperatures ◽  
Droplet Vaporization ◽  
Flux Model ◽  
Heat Flux Model

A model for unsteady droplet vaporization is presented that considers the droplet temperature range from flash-boiling conditions to normal evaporation. The theory of continuous thermodynamics was used to model the properties and compositions of multicomponent fuels such as gasoline. In order to model the change of evaporation rate from normal to boiling conditions more realistically, an unsteady internal heat flux model and a new model for the determination of the droplet surface temperature is proposed. An explicit form of the equation to determine the heat flux from the surrounding gas mixture to the droplet-gas interface was obtained from an approximate solution of the quasi-steady energy equation for the surrounding gas mixture, with the inter-diffusion of fuel vapor and the surrounding gas taken into account. The model was applied to calculate evaporation processes of droplets for various ambient temperatures and droplet temperatures.

scholarly journals Early differentation of lamellar structure of the intervertebral disc in staged human embryos

2015 ◽  
Vol 84 (3) ◽  
pp. 157-166
Author(s):  
Witold Woźniak ◽  
Małgorzata Grzymisławska ◽  
Joanna Łupicka ◽  
Małgorzata Bruska ◽  
Adam Piotrowski ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Annulus Fibrosus ◽  
Developmental Stages ◽  
Intervertebral Discs ◽  
Human Embryos ◽  
Lateral Zone ◽  
Vast Literature ◽  
Dense Zone

Introduction. In the vast literature concerning the development of the intervertebral discs controversies exist as to the period of differentiation and structure of the nucleus pulposus and annulus fibrosus. These controversies result from different determination of age of the investigated embryos. Aim. Using embryos from departmental collection age of which was established according to international Carnegie staging and expressed in postfertilizational days, the differentiation of the intervertebral discs was traced. Material and methods. Study was performed on 34 embryos at developmental stages 13–23 (32–56 days). Embryos were serially sectioned in sagittal, frontal and horizontal planes. Sections were stained with various histological methods and impregnated with silver.Results. Division of sclerotomes into loose cranial and dense caudal zones (sclerotomites) was observed in embryos aged 32 days (stage 13). The intervertebral disc developed from the dense zone of sclerotome and was well recognized in embryos aged 33 days (stage 14). At the end of fifth week (embryos at stage 15, 36 days) the annulus fibrosus and the nucleus pulposus were seen. The annulus fibrosus differentiated into lateral and medial zones. Within the lateral zone cells were arranged into circular rows. These rows were considered as the first stage of laminar structure. In further developmental stages the laminae occupied both zones of the annulus fibrosus.Conclusions. The intervertebral discs develop from the dense zone of the sclerotome which is evident in embryos at stage 13 (32 days). Discs differentiate in embryos aged 33 days, when the nucleus pulposus and annulus fibrosus are recognized. In embryos aged 36 days in the annulus fibrosus circular rows forming laminar arrangement are seen.

scholarly journals DEVELOPMENT OF Chrysomya albiceps WIEDEMANN, 1819 (DIPTERA: CALLIPHORIDAE) AND Musca domestica LINNAEUS, 1758 (DIPTERA: MUSCIDAE) REARED TOGETHER IN PIG TISSUES.

2019 ◽  
Vol 48 (1) ◽  
pp. 46-60
Author(s):  
Cíntia De Campos Chaves ◽  
Manuela Campos ,Ferreira ◽  
Júlio Mendes
Keyword(s):  
Musca Domestica ◽  
Trophic Interactions ◽  
Forensic Entomology ◽  
Animal Health ◽  
Growth Time ◽  
Chrysomya Albiceps ◽  
Pure Cultures ◽  
Successful Colonization ◽  
Shed Light ◽  
Better Than

Chysomya albiceps and Musca domestica are important for forensic entomology, and human and animal health. This study analyzed the effects of the coexistence of C. albiceps and M. domestica reared in four different assays in two pig tissues, brain and intestine: assay 1, interaction between the larvae of the same age; assay 2, interaction between larvae of C. albiceps 24 hours older than larvae of M. domestica; assay 3, interaction between larvae of M. domestica 24 hours older than the larvae of C. albiceps; assay 4, larvae of both species were reared together in flasks with a small supply of food. Weight of larvae, growth time and imago emergence frequency were studied. C. albiceps responded better than M. domestica under most conditionstested. Larvae of C. albiceps responded better in mixed cultures (together with M. domestica) than in pure cultures (larvae of the same species). In contrast, M. domestica responded better when reared in pure cultures. Both species presented shorter growth times when their larvae were reared in intestine tissue with larvae 24 hours younger than the larvae of the concurrent species, compared to their respective growth times in pure cultures. The results confirmed that trophic interactions are relevant to the successful colonization of carrion by C. albiceps. Coexistence of the two species may result in changes in values of their biological components. The results also help to shed light on the biology of the two species in carrion.

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