scholarly journals UV irradiation-promoting effect on the antibacterial activity of cyanobacterial extracts against plant pathogens: a first record

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Munirah Aldayel ◽  
Nermin El Semary
Keyword(s):  
Antibacterial Activity ◽  
Uv Irradiation ◽  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Antimicrobial Effect ◽  
Uv Exposure ◽  
Eastern Region ◽  
Main Body ◽  
Bacterial Strains ◽  
Promoting Effect

Abstract Background Cyanobacteria possess a wide array of bioactive compounds including those with antimicrobial activities. The target was to investigate the UV effect on antimicrobial activity of cyanobacterial extracts. Several cyanobacterial strains were isolated from Eastern region of KSA as well as three plant pathogenic bacterial strains. Four cyanobacterial strains were used. Two strains were isolated from Al Uquair region, Arabian Gulf, and identified as Synechococcus sp. and Oscillatoria sp. The two other strains were collected from brackish stream of underground water and were characterized as Synechocystis sp. and Phormidium sp. The antimicrobial bioassay was then performed using cyanobacterial aqueous extracts. The antimicrobial effect was estimated by measuring the inhibition zone compared to that of control. The extract was divided into 2 parts: part was not exposed to UV and the other was exposed to UV-B irradiation for 10 min. The antimicrobial bioassay was performed for both parts of the extract, using plant pathogenic bacteria, namely, Erwinia sp., Pseudomonas sp., and Bacillus sp. Main body The antimicrobial profile was examined and results showed that the extracts showed non-antimicrobial effect before UV irradiation, and antimicrobial effect after UV exposure. Also, those that were active against pathogenic bacteria were more active after UV exposure. This is mostly attributed to a high optical energy of UV irradiation that subsequently had a significant impact on the electron transitions in the molecules of the extract rendering some of them more effective in their antibacterial action. Conclusion This short communication was the first report where the UV can alter the antimicrobial profile of cyanobacterial extracts. This is a novel approach in enhancing antibacterial activity. Future molecular investigations will be conducted to further characterize the isolate whose extract showed the highest response to UV treatment.

scholarly journals Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 98 ◽  
Author(s):  
Eunice Mgbeahuruike ◽  
Pia Fyhrquist ◽  
Heikki Vuorela ◽  
Riitta Julkunen-Tiitto ◽  
Yvonne Holm
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Hot Water ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Piper Guineense ◽  
E Coli ◽  
Growth Inhibitory ◽  
Derivatives Of

Piper guineense is a food and medicinal plant commonly used to treat infectious diseases in West-African traditional medicine. In a bid to identify new antibacterial compounds due to bacterial resistance to antibiotics, twelve extracts of P. guineense fruits and leaves, obtained by sequential extraction, as well as the piperine and piperlongumine commercial compounds were evaluated for antibacterial activity against human pathogenic bacteria. HPLC-DAD and UHPLC/Q-TOF MS analysis were conducted to characterize and identify the compounds present in the extracts with promising antibacterial activity. The extracts, with the exception of the hot water decoctions and macerations, contained piperamide alkaloids as their main constituents. Piperine, dihydropiperine, piperylin, dihydropiperylin or piperlonguminine, dihydropiperlonguminine, wisanine, dihydrowisanine and derivatives of piperine and piperidine were identified in a hexane extract of the leaf. In addition, some new piperamide alkaloids were identified, such as a piperine and a piperidine alkaloid derivative and two unknown piperamide alkaloids. To the best of our knowledge, there are no piperamides reported in the literature with similar UVλ absorption maxima and masses. A piperamide alkaloid-rich hexane leaf extract recorded the lowest MIC of 19 µg/mL against Sarcina sp. and gave promising growth inhibitory effects against S. aureus and E. aerogenes as well, inhibiting the growth of both bacteria with a MIC of 78 µg/mL. Moreover, this is the first report of the antibacterial activity of P. guineense extracts against Sarcina sp. and E. aerogenes. Marked growth inhibition was also obtained for chloroform extracts of the leaves and fruits against P. aeruginosa with a MIC value of 78 µg/mL. Piperine and piperlongumine were active against E. aerogenes, S. aureus, E. coli, S. enterica, P. mirabilis and B. cereus with MIC values ranging from 39–1250 µg/mL. Notably, the water extracts, which were almost devoid of piperamide alkaloids, were not active against the bacterial strains. Our results demonstrate that P. guineense contains antibacterial alkaloids that could be relevant for the discovery of new natural antibiotics.

scholarly journals INVESTIGATION OF THE SYNERGISTIC ANTIBACTERIAL ACTION OF COPPER NANOPARTICLES ON CERTAIN ANTIBIOTICS AGAINST HUMAN PATHOGENS

2018 ◽  
Vol 10 (10) ◽  
pp. 83
Author(s):  
Selvarani Murugan
Keyword(s):  
Antibacterial Activity ◽  
Copper Nanoparticles ◽  
Pathogenic Bacteria ◽  
Color Change ◽  
Quantitative Information ◽  
Inorganic Nanoparticles ◽  
Diffusion Method ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Agar Disc

Objective: Resistance to antibacterial agents by pathogenic bacteria has emerged in recent years and is a major challenge for the healthcare industry. Copper nanoparticles (CuNPs) are known to be one of the multifunctional inorganic nanoparticles with effective antibacterial activity. Hence the present investigation has been focused on synthesizing and evaluating the bactericidal effect of copper nanoparticles.Methods: CuNPs were synthesized by reducing the aqueous solution of copper sulfate with sodium borohydride. The synthesized particles were characterized by x-ray diffractogram (XRD), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) techniques to analyze size, morphology and quantitative information respectively. The antibacterial activity of CuNPs was examined by agar well diffusion method. Synergistic effect of CuNPs with broad-spectrum antibiotics was determined by the agar disc diffusion method.Results: Color change of reaction mixture from blue to dark brown indicated the formation of CuNPs. SEM image clearly demonstrated that the synthesized particles were spherical in shape and its size was found to be 17.85 nm. EDS report confirmed the presence of elemental copper in the resultant nanoparticles and its accounts for major proportion (96%) of the mass of nanoparticles. Bacterial effect of CuNPs revealed that Pseudomonas aeruginosa showed the highest antibacterial sensitivity (16.00±1.63 mm), whereas least susceptibility (9.67±0.47 mm) was noticed against Staphylococcus aureus. An enhanced antibacterial activity of commercial antibiotics was also noticed when it combined with CuNPS. A minimum zone of inhibition was increased from 0.67±0.47 mm to 10.66±0.24 mm when the nanoparticles and antibiotics were given together.Conclusion: It was observed that copper nanoparticles exhibited profound activity against all the tested bacterial strains which shows that CuNPs may serve as a better option for use in medicine in the future.

scholarly journals Purification and Characterization of Antibacterial Activity against Phytopathogenic Bacteria in Culture Fluids from Ganoderma lucidum

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5553
Author(s):  
Loreto Robles-Hernández ◽  
Nora A. Salas-Salazar ◽  
Ana C. Gonzalez-Franco
Keyword(s):  
Antibacterial Activity ◽  
Ganoderma Lucidum ◽  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Heat Stability ◽  
Limited Information ◽  
Dialysis Tubing ◽  
Molecular Weights ◽  
Exclusion Chromatography

Previous studies of Ganoderma lucidum have focused on its medicinal applications. Limited information is available about its antibacterial activity against plant pathogens. Thus, the goal of this study was to purify and characterize the antibacterial activity against plant pathogenic bacteria from culture fluids of G. lucidum. The nature of the bioactive components was determined using heat boiling, organic solvents, dialysis tubing, gel exclusion chromatography (GEC), proteinase sensitivity, HPLC, HPLC-APCI-MS, and GC-MS. The bioactive compounds were neither lipid, based on their solubility, nor proteic in nature, based on proteinase digestion and heat stability. The putative-bioactive polysaccharides have molecular weights that range from 3500 to 4500 Daltons as determined by dialysis tubing, GEC and APCI-MS analysis. The composition of the antibacterial compounds was determined by GC-MS. This is the first report of small polysaccharides produced by G. lucidum with activity against bacterial plant pathogens.

Synthesis and Antibacterial Activities of Amidine Substituted Monocyclic β-Lactams

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijuan Zhai ◽  
Lili He ◽  
Yuanbai Liu ◽  
Ko Ko Myo ◽  
Zafar Iqbal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Lead Target ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Clinical Interest

Background: Mononcyclic β-lactams are regarded as the most resistant class of β-lactams against a series of β-lactamases though possess limited antibacterial activity. Aztreonam being the first clinically approved monobactam needs broad-spectrum efficacy through structural modification. Objective: We strive to synthesize a number of monocyclic β-lactams by varying the substituents at N1, C3 and C4 positions of azetidinone ring and study the antimicrobial effect on variable bacterial strains. Methods: Seven new monobactam derivatives 23a-g, containing substituted-amidine moieties linked to the azetidinone ring via thiazole linker, were synthesized through multistep synthesis. The final compounds were investigated for their in vitro antibacterial activities using broth microdilution method, against ten bacterial strains of clinical interest. The minimum inhibitory concentrations (MICs) of newly synthesized derivatives were compared with aztreonam, ceftazidime and meropenem, existing clinical antibiotics. Results: All compounds 23a-g showed higher antibacterial activities (MIC 0.25 µg/mL to 64 µg/mL) against tested strains as compared to aztreonam (MIC 16 µg/mL to >64 µg/mL) and ceftazidime (MIC >64 µg/mL). However all compounds, except 23d, exhibited lower antibacterial activity against all tested bacterial strains as compared to meropenem. Conclusion: Compound 23d showed comparable or improved antibacterial activity (MIC 0.25 µg/mL to 2 µg/mL) to meropenem (MIC 1 µg/mL to 2 µg/mL) in case of seven bacterial species. Therefore, compound 23d may be valuable lead target for further investigations against multi-drug resistant Gram-negative bacteria.

Phytochemical Screening, Antibacterial and Synergistic activity of Dittrichia Viscosa Extracts against Multi-Resistant Pathogenic Bacteria

2019 ◽  
Vol 2 (1) ◽  
pp. 01-06
Author(s):  
Rhazi Fouzia
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Synergistic Activity ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Dittrichia Viscosa ◽  
Study Results ◽  
Ethanolic Extracts

Study contextual: Faced with the global problem of antimicrobial resistance, and the use of traditional medicine for the research of antibacterial biomolecules. Aim: our work focused on the valorization of a medicinal plant Dittrichia viscosa which has many therapeutic and culinary virtues worldwide. Methods: To do this, a phytochemical screening of the leafy stems of the plant is carried out according to a set of physicochemical reactions, as well as an in vitro evaluation of the antibacterial activity, of the aqueous and ethanolic extracts against multi-resistant bacterial strains, by microdilution technique on microplates. An evaluation of the synergistic interaction between extracts and weakened antibiotics against pathogenic bacteria was also highlighted in this study. Results: The tests revealed the richness of Dittrichia viscosa species by tannins, flavonoids, saponosides, sterols and triterpenes. As for the antibacterial effect, the MICs range from 0.858±0.29 to 66.66 ± 0.00 mg / ml and the MBCs from 4.300 ± 1.01 to 11.610 ± 2.31 mg / ml is an interesting antibacterial activity. Regarding the combination of extracts with antibiotics tested, it revealed a synergistic action inducing an amplification of the antibacterial power of Penicillin, Imipenem and Erythromycin with a rate that reaches 471%. Conclusion: The results of this study show that the aqueous and ethanolic extracts of Dittrichia viscosa have interesting and promising antibacterial activity in the pharmaceutical, food and cosmetic industries.

Antimicrobial Effect of Brazillian Propolis/Polycaprolactone Polymer on some Human Pathogenic Bacteria

2012 ◽  
Vol 506 ◽  
pp. 537-540 ◽  
Author(s):  
S. Sanpa ◽  
Krit Sutjarittangtham ◽  
Tawee Tunkasiri ◽  
Sukum Eitssayeam ◽  
P. Chantawannakul
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Electrospinning Technique ◽  
Propolis Extract ◽  
Human Pathogenic Bacteria ◽  
Polymer Nanofiber ◽  
History Of Use

The antimicrobial activities of propolis against some bacterial strains have been studied. However, there are few reports on biopolymers from propolis. Polycaprolactone (PCL) is a biodegradable polymer that has a long history of use in the biomedical field. In this work, the polymer nanofiber was grown from a Brazillian propolis extract solution on PCL using an electrospinning technique. Antimicrobial activities were investigated by the disc diffusion method. The propolis extract and polymer nanofiber could inhibit some human pathogenic bacteria. In addition, the inhibitory effects on tested microorganism depended on the concentration of propolis extract mixed with the polymer nanofiber. Such properties of the propolis/PCL nanofiners can be further developed for biomedical applications.

scholarly journals Some endemic medicinal plants of Andamans with antimicrobial potential

2016 ◽  
Vol 1 (1) ◽  
pp. 10
Author(s):  
Asma Bibi ◽  
A. Thangamani ◽  
V. Venkatesalu
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Pathogenic Bacteria ◽  
Phytopathogenic Fungi ◽  
Gram Negative Bacteria ◽  
Andaman Islands ◽  
Bacterial Strains ◽  
Endemic Plant ◽  
Ethanol Extracts ◽  
Endemic Plant Species

The present study was aimed to determine the antimicrobial activity of some endemic plant species used in folkloric medicine by the inhabitants of Andaman Islands, India. The ethanol extracts prepared from the leaves of four plants viz; Alstonia kurzii, Tabernaemontana crispa, Mangifera andamanica and Vitex diversifolia were assessed for antibacterial activity against clinically isolated human pathogenic bacteria and antifungal activity against some phytopathogenic fungi. The ethanol extracts showed more inhibition towards Gram positive than Gram negative bacteria and the bacterial strains showed more susceptibility than the fungal strains tested. Among the plants, Vitex diversifolia exhibited the highest antibacterial activity and Mangifera andamanica showed the highest antifungal activity.

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

scholarly journals STUDY CYTOTOXICITY, ANTIBACTERIAL ACTIVITY, AND COMPOSITIONS BY FT-IR AND GC-MS FOR ORGANIC EXTRACT OF WHITE IRAQI DESERT TRUFFLE TIRMANIA NIVEA

2020 ◽  
Vol 17 (36) ◽  
pp. 18-31
Author(s):  
Ahmad khadem HACHIM ◽  
Rashid Rahim HATEET ◽  
Tawfik Muhammad MUHSIN
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Cell Line ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Organic Extract

The purpose of the present work aimed at exploring the potential biochemical components and biological activities of an organic extract of the white truffle Tirmania nivea collected from the Iraqi desert, then test the organic extract against the Cytotoxicity on Human Larynx carcinoma cells and selected strains of pathogenic bacteria. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry GC/MSS were used to analyze mycochemical compositions. The antibacterial activity and Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was investigated using a disk diffusion agar method. The truffle extract's cytotoxicity effect against the larynx cell line (Hep-2) was assessed by the MTT assay (in vitro). FTIR results provided the presence of phenol, carboxylic acid, and alkane's functional group, The GC-MS analysis of T. nivea disclose the existence of nineteen compounds that can contribute to the pharmaceutical properties of the truffle. As for antibacterial activity result, A growth inhibition activity of truffle extract at (18-40 mm inhibition zones) against the tested pathogenic bacterial strains was detected, which minimum inhibitory concentration values ranged from 3.12 to 6.25 mg/mL for Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) Respectively. The results of cytotoxicity shown that the organic truffle extract exhibited a high inhibitory rate (52.685%) against cell line (Hep-2) at a concentration of 1.56 ?g/mL. In this work, the results showed that the organic extracts of T. nivea are very promising as cancer cytotoxicity and antibacterial agent for future medical applications.

scholarly journals In Vitro Assessment for Antimicrobial Activity of Lactobacillus Helveticus and its Natural Glycopeptides Against Mastitis Causing Pathogens in Dairy Cat

2015 ◽  
Vol 9 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Gundega Gulbe ◽  
Anda Valdovska ◽  
Vaira Saulite ◽  
Jevgenijs Jermolajevs
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Lactobacillus Helveticus ◽  
Haemolytic Activity ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Coagulase Negative Staphylococci

Probiotic lactic acid bacteria have a great potential to control bovine mastitis as well as they are favourable choice to treat many infectious diseases of human. These bacteria are well known as having many properties which make them beneficial to control pathogenic microorganisms. These include, the ability to adhere to cell, the reduction of pathogenic bacteria adherents, the co–aggregation, the production of organic acids, hydrogen peroxide, bacteriocin and etc., to be safe and non–pathogenic, which antagonize pathogenic microorganisms. However, each strain must be well identified and characterized in vitro before using for disease treatment. The aim of the present study was to screen three kind of test suspensions: TS1, TS2 and TS3, which contains probiotic lactic acid bacterium Lactobacillus helveticus or its natural glycopeptides, and other natural immunomodulators, in order to investigate which content were the most effective in inhibiting several mastitis causing bacteria in dairy cattle: coagulase–positive Staphylococcus aureus, coagulase–negative staphylococci S. haemolyticus, S. saprophyticus, S. simulans, S. vitulinus, and Gram–negative bacteria Citrobacter freundii and Serratia liquefaciens. Test suspensions TS1, TS2 and TS3 were adjusted by pH 6.3, then tested in vitro by well diffusion assay to determine their antimicrobial effect against bacteria. Furthermore haemolytic activity of applied test suspensions were determined. In results TS1 (9-13 mm) and TS2 (10-15 mm) showed the inhibition effect on four of eight tested bacterial strains, whereas TS3 did not displayed any antimicrobial effect. TS2 have a greatest antimicrobial activity as they resulted in the largest inhibition zones.

Sign in / Sign up

Export Citation Format

Share Document